We narrowed to 770 results for: Mos;
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TypeCollection...Parasites such as Plasmodium (malaria), Toxoplasma (toxoplasmosis), Trypanosoma (African sleeping sickness), Cryptosporidium...homology-directed repair (HDR) instead of NHEJ. In most experimental systems, HDR occurs at a much lower...
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CRISPR Plasmids - CRISPR Transposases (CAST)
TypeCollection...exhibit almost unidirectional insertions, but are more prone to off-target insertions. CASTs are most often... -
DNA Service - Cloning Grade DNA
TypeCollection...by ordering cloning grade DNA for over 200 of our most popular plasmids!...but helps to inform us of which plasmids would be most helpful to requesting scientists. Return to top ... -
Chemogenetics Plasmids
TypeCollection...Selective Actuator Modules (PSAMs). DREADDs are the most widely used chemogenetic receptors and are G-coupled...recently developed class of tools are Luminopsins (LMOs), or luminescent opsins: fusion proteins of a light-emitting... -
Hot Plasmids May 2018 - Optogenetics, Decaffeination, Biosensors, and Fluorescent Protein Tools
TypeBlog Post...work in your lab. Find the FLINC biosensors here. Mo et al. Nat Methods. 2017. PubMed PMID: 28288122. ... -
Synthetic Biology - Assembly Standards Guide
TypeCollection... provides information for the assembly standards most commonly used by part registries, including the ... Cloning Techniques Guide for an overview of the most common methods. BioBrick BioBrick-2 BglBrick Silver... -
Adenovirus Plasmids
TypeCollection...range of dividing and non-dividing cell types with almost 100% efficiency. Unlike lentiviruses or other retroviruses...Adenoviruses can be produced by several methods, the most common of which involves homologous recombination... -
p53 Pathway
TypeCollection...Cancer Impact p53 is the most frequently mutated gene (>50%) in human cancer; most of these mutations are... -
TALEN Guide
TypeCollection...region of DNA in the genome of your favorite species almost as easily as correcting a typo in the latest draft...make arrays from 12-31 repeats. Perhaps one of the most appealing features of the TAL effector arrays is... -
Ras Pathway
TypeCollection...small GTPase and constitutively active Ras is the most common oncogene in human cancer....leading to cancer. Constitutively active Ras is the most common oncogene in human cancer, found in around... -
Empty Backbones - Choosing Your Perfect Plasmid Backbone
TypeCollection...selection of Addgene's empty vector backbones. For the most part, we will assume that you want to express a ... - An improved agroinfection-compatible Tobacco mosaic virus (TMV)-based transient expression vector GFP... -
CRISPR History and Development for Genome Engineering
TypeCollection... The beauty of CRISPR is that it’s adaptable to almost any model system or biological topic, and you don...Pederson T. 2015. Multicolor CRISPR labeling of chromosomal loci in human cells. Proc Natl Acad Sci U S A... -
Bacterial Expression Systems
TypeCollection...interesting biological questions. Escherichia coli is the most widely used bacterial organism due to its ease of...contamination and facilitates extraction. Browse our most popular plasmids for protein purification. Commonly... -
Tetracycline Inducible Expression
TypeCollection...targets. The tetracycline (tet) system is one of the most popular choices for rapid, efficient, and reversible...sequences with elements like the H1 RNA promoter. And, most importantly, the TetR protein has been modified ... -
Fluorescent Protein Guide: Subcellular Localization
TypeCollection...determining subcellular localization. One of the most common is to use fluorescence microscopy to compare...junctions, gap junctions, tight junctions, and desmosomes. Browse or search the table below to find markers... -
Viral Vectors 101: Pseudotyping
TypeBlog Post...Bieniasz PD, Jäger U, Porter CD, Friedman T, McClure MO, Weiss RA (1997) Sensitization of rhabdo-, lenti-...so commonly used is that it is good at infecting most cells, but not all. For example, LDLR expression...envelope proteins for pseudotyping While VSV-G is the most commonly used envelope protein for pseudotyping,... -
Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone
TypeBlog Post...Susanna Harris, Heather Hendrickson, Jonathan Jacobs, Mo Kaze, Christina Kellogg, Heather Martinez, Pat Schloss...don’t have the networks or experience to get the most of meeting. I experienced this at the ASM General...and helping societies help their members get the most out of their meetings. Amanda Gunn ran randomized... -
Chemogenetics Guide
TypeGuide...Silvagnoli AD, Crespo EL, Schalau R, Gott M, Tree MO, Dunbar GL, Rossignol J, Hochgeschwender U (2025)...Gorantla N, Simkins J, Crespo EL, Ikefuama EC, Tree MO, Prakash M, Björefeldt A, Barnett LM, Lambert GG,...Addgene's collection of PSAM plasmids ! LMOs Luminopsins (LMOs), or luminescent opsins, are opto-chemogenetic...Early Chemogenetic Receptors: RASSLs DREADDs PSAMs LMOs Planning Your Experiment References Chemogenetic... channels targeted to specific cell populations (most often neurons) that can be turned on or off by the...have few, if any off-target effects. They are the most widely employed type of chemogenetic receptors. ...: hM1Dq, hM3Dq, and hM5Dq, however, hM3Dq is the most widely employed. Gi-DREADDs signal through the Gαi... -
iPSC Neurodegenerative Disease Initiative Plasmid Collection
TypeCollection...Link opens in a new window) at the NIH. Reference Ramos et al. 2021. Tackling neurodegenerative diseases... -
Cancer Research Plasmids and Resources
TypeCollection... regulating the growth of the cell. p53 p53, the most frequently mutated gene in human cancer, is a transcription...