We narrowed to 556 results for: SEC

...organisms, ranging from bacteria to aquatic animals to insects. Luciferase catalyzes the oxidation of a substrate...kDa and 36 kDa, respectively). And they are not secreted, so cells must be lysed or the substrate must ...to measure luminescence. Gaussia luciferase is secreted and more stable, enabling time course experiments... kDa), very bright, and ATP-independent, and a secreted version of the protein is available, making it...ATP Timothy Ryan 24348 pAC-hRluc Renilla Actin5C Insect expression of Renilla luciferase Liqun Luo 51970...181934 pC0037 Gaussia, Cypridina EF1α, CMV Dual secreted luciferase reporter (EF1α-Gaussia luciferase, ...

...in bacteria, mammalian cells, yeast, plants, or insect cells. Benefits include consistency between lots...mammalian tissue culture cells, the R-mAbs are secreted into the culture media and can be collected for...domains to obtain recombinant hybridomas, which secrete antibodies as Fab' fragments, isotype-switched ...antibodies against zebrafish neural receptors and secreted proteins suitable for wholemount immunostaining...mCherry Monoclonals, Nanobodies, Sybodies, or ScFVs Secondary antibodies (search for "anti-mouse" or "anti-rabbit...

...express a gene; however, we have included a function section for if you want to stably express an insert using...vector pFA6a vectors - Epitope tagging in S. pombe Insect/Baculovirus UAS, MT, Polyhedrin Targeted Gene ...LIC - Vectors from the Gradia lab pFB-LIC-Bse - Insect expression vector from Gileadi lab Worm unc-54,...bacterial expression (Gateway) pYD1 - Expression, secretion, and display of proteins on the extracellular ...pFastBac LIC vectors and pFastBac Dual LIC vectors for insect expression Fluorescent proteins (GFP, mCherry, ...

... and a species-specific fluorescently-labeled secondary antibody. The fluorescence pattern is compared...untransfected control. Immunohistochemistry (IHC) Tissue sections are fixed, permeabilized, and labeled with the...species-specific horseradish peroxidase (HRP)-conjugated secondary antibody. The tissue labeling pattern is compared...antibody and a species-specific HRP-conjugated secondary antibody. Protein expression is compared to an...

...Voeltz 49155 mCh-Sec61 beta Endoplasmic reticulum Sec61 mCherry Gia Voeltz 49154 BFP-Sec61 beta Endoplasmic...Collection . Return to top Membrane Trafficking The secretory pathway is responsible for delivering proteins...Endoplasmic reticulum Sec61 TagBFP Gia Voeltz 231776 pCMV-mGold2t-Calnexin-N-14 Endoplasmic reticulum Calnexin...

... packaging and regulatory genes; one plasmid in second-generation, two plasmids in third-generation Envelope...packaging gene env This collection highlights popular second- and third-generation lentiviral packaging, envelope...4070A Env GP. Good for work in human MSCs. Use with second-generation packaging construct encoding tat . Jakob...of VSV-G glycoprotein (Indiana strain). Use with second-generation packaging construct encoding tat . Jakob...

...Arfgef3 (BIG3) 57221 2177 GEF Iqsec1 (BRAG2, GEP100) 9922 1114 Gateway GEF Iqsec2 (BRAG1) 23096 1488 Bacterial...Bacterial (pET) GEF Iqsec3 440073 1182 GEF Cyth1 9267 398 Gateway GEF Cyth2 (ARNO) 9266 400 Mammalian (pEGFP-C1...

... been designed for use in Drosophila and other insects.... been designed for use in Drosophila and other insects. To find emtpy gRNA plasmids, visit Addgene's Empty...

...AmpR 26095 pFHMSP-LIC-N N/A Expression vector for secreted proteins with N-terminal hexahistidine tag and...AmpR 26100 pFHMSP-LIC-C N/A Expression vector for secreted proteins with C-terminal hexahistidine tag and...

...experimental details in the Antibody Applications section of our product pages. We also include negative ...affinity reagents such as scFvs, DARPins, Fabs, and secondary antibodies. To learn more about antibodies and...

...future publications. For your Materials and Methods section: "The plasmid kit used in this publication was ...(Addgene kit # 1000000013)" For your Reference section: Oligomerized pool engineering (OPEN): an 'open-source...

... Beta-actin Actin filaments 87426 SEC61B-mEGFP AICSDP-7 mEGFP Sec61 beta Endoplasmic reticulum 87427 FBL-mEGFP...

...CRISPR in your own research. CRISPR Basics In this section, we will introduce the basic mechanisms of CRISPR...Generating a Knockout Using CRISPR Cas9 undergoes a second conformational change upon target binding. In this...have been developed, including editors based on a second ADAR variant that displays improved specificity...Oost, J. (2012). CRISPR immunity relies on the consecutive binding and degradation of negatively supercoiled...

...and their corresponding Cas enzymes. In the next section, we will cover strategies for designing the gRNA...Friedman, N., & Regev, A. (2016). Perturb-Seq: Dissecting Molecular Circuits with Scalable Single-Cell ...

...channelrhodopsin, which enables single cell, single millisecond resolution optogenetics. Human Synapsin (hSynapsin...channelrhodopsin, which enables single cell, single millisecond resolution optogenetics. Cre-dependent virus ...

...M. barkeri photocrosslinkers DiZPK, DiZSeK, or DiZHSeC Bacterial TAG P. Chen 91706 pCMV-MbPylRS(DiZPK)...M. barkeri photocrosslinkers DiZPK, DiZSeK, or DiZHSeC Mammalian TAG P. Chen 92047 pCOTS-pyl-GFP(35TAG...

..., or others, it will be listed on the Function section pages above. You can also do a search of viral ...

... 0, 10, 11, 14, or 20 matching nucleotides, the second binds to mcherry with either 0, 11, 18, or 20 matching...

... of the dual guide shuttle plasmids to insert a second guide within a U6-guide cassette. This process ...

...with a corresponding primary and dye-conjugated secondary antibody (as listed in Park & Sheridan et al.,...