...2015. 17(2): p. 178-94. PubMed PMID: 26189426. 15. Si-Tayeb, K., et al., Highly efficient generation of...successfully [10-12]. The next stage of reprogramming consists of redifferentiation of iPSCs into the cell type...

...Brown W, Bardhan A, Delaney M, Ilk AS, Rauen RR, Kahn SI, Tsang M, Deiters A (2020) Spatiotemporal Control...protein combination. The optimized LACE system consisted of: 1) CIBN-dCas9-CIBN, where CIBN is the N-terminal...(Kawano et al., 2015). Nicknamed paCas9-1 and consisting of the fusion proteins N713-pMag and nMagHigh1...Michael Lin, who developed a chimeric protein consisting of Cas9 and a photo-dissociable dimeric green...

...simian sarcoma viral (v-sis) oncogene homolog) FLJ12858, PDGF2, SIS, SSV, c-sis PDGFC platelet derived ...PTP, REG RETN resistin ADSF, FIZZ3, MGC126603, MGC126609, RETN1, RSTN, XCP1 RETNLB resistin like beta FIZZ1...tumor necrosis factor receptor superfamily, member 12A CD266, FN14, TWEAKR TNFRSF13B tumor necrosis factor... tumor necrosis factor receptor superfamily, member 17 BCM, BCMA, CD269 TNFRSF18 tumor necrosis factor...tumor necrosis factor receptor superfamily, member 21 BM-018, DR6, MGC31965 TNFRSF25 tumor necrosis factor...tumor necrosis factor receptor superfamily, member 8 CD30, D1S166E, Ki-1 TNFRSF9 tumor necrosis factor...cell differentiation, and apoptosis. Symbol Name Synonyms TNFRSF10B tumor necrosis factor receptor superfamily...

...amino acid linker ACA Heterotrimeric construct consisting of Amber-5aa linker-Cerulean-6aa linker-Amber...linker-Amber ACV Heterotrimeric construct consisting of Amber-5aa linker-Cerulean-6aa linker-Venus VCA Heterotrimeric...Heterotrimeric construct consisting of Venus-5aa linker-Cerulean-6aa linker-Amber VCV Heterotrimeric construct...construct consisting of Venus-5aa linker-Cerulean-6aa linker-Venus ACAV Heterotetrameric construct consisting... linker-Venus ACVA Heterotetrameric construct consisting of Amber-5aa linker-Cerulean-5aa linker-Venus... linker-Amber VCAA Heterotetrameric construct consisting of Venus-5aa linker-Cerulean-5aa linker-Amber... linker-Amber VCVV Heterotetrameric construct consisting of Venus-5aa linker-Cerulean-5aa linker-Venus...

...those regions. Locus-specific ChIP technologies consist of insertional chromatin immunoprecipitation (iChIP...molecules such as TAL proteins and the CRISPR system consisting of a catalytically inactive form of Cas9 (dCas9...expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest...Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest...

...seed and other stocks of Arabidopsis thaliana and related species. The Arabidopsis Information Resource (...gene expression profiles in Arabidopsis thaliana based on RNA-seq analysis. Return to top Do you have ...tools for research using plant systems such as Arabidopsis, wheat, maize, rice, tomato, and a variety of...in a variety of plant species (e.g. maize and Arabidopsis) and vectors for easy assembly of one or more...Cas9 assembly for fast generation of multiple Arabidopsis mutants - Niko Geldner. CRISPR-Cas9 vector with...Cloning (JMC) Toolkit includes a modular cloning chassis and set of functional parts for assembling plant...designed for gene silencing in plants: microRNAs in Arabidopsis - Detlef Weigel. microRNAs in rice - Detlef Weigel...

...California, Berkeley. CRISPR Software Sanger Indel Analysis ICE (Inference of CRISPR Edits) This new open ...preprint describing ICE . Deep Sequencing Data Analysis CRISPResso CRISPResso is a robust and easy-to-... and quantify the efficiency of the targeted mutagenesis The amplicon sequence expected after HDR can ...experiments. PubMed PMID 27404874 . MAGeCK Model-based Analysis of Genome-wide CRISPR/Cas9 Knockout (MAGeCK). ...CrispyCrunch is used for high-throughput design and analysis of CRISPR + HDR experiments. For more information...off-target binding. Currently supports: Drosophila, Arabidopsis, zebrafish, C. elegans, mouse, human, rat, cow...inactivation, NHEJ, and HDR. Reference genomes include Arabidopsis, C. elegans , sea squirt, cavefish, Chinese hamster...

...agonist CASP3 Caspase 3, apoptosis-related cysteine peptidase CASP8 Caspase 8, apoptosis-related cysteine peptidase...effects of p53 are in promoting cell cycle arrest, apoptosis, or senescence in damaged cells. The p53 name ...pro-apoptotic effects is especially important to tumorigenesis. Inheriting only one functional copy of the ... 1; also known as GTSE1 BAI-1 Brain-specific angiogenesis inhibitor 1 Bax BCL2-associated X protein Bid...peptidase CASP9 Caspase 9, apoptosis-related cysteine peptidase Cyclin B CCNB1 CCNB2 CCNB3 Cyclin B1, ...ubiquitin protein ligase cytoC Cytochrome c DR5 Tumor necrosis factor receptor superfamily, member 10b E2F-1 ...protein p53 p53AIP1 Tumor protein p53 regulated apoptosis inducing protein 1 p53R2 p53 inducible, ribonucleotide...

...Endo/Exocytosis Superecliptic pHluorin fused to glutamate receptor Glutamate receptor exocytosis and spine...Robert Malinow Endo/Exocytosis Synaptophysin-pHluorin Clathrin-mediated endocytosis is the dominant mechanism...Synaptic vesicle pH sensors for imaging exocytosis and endocytosis (pHuji). Semisynthetic fluorescent pH...pH sensors for imaging exocytosis and endocytosis. Nat Commun. 2017 Nov 10;8(1):1412. Justin Taraska ,...Rise in Free Mg(2+) Ions Released from ATP-Mg Hydrolysis Contributes to Mitotic Chromosome Condensation...FRET sensors to monitor intracellular Zn(2+) homeostasis. Nat Methods. 2009 Oct;6(10):737-40. Maarten ... ATP sensor for Yeast (AT1.03 derived) Dynamic analysis of cytosolic glucose and ATP levels in yeast using...

...( nicks ). ID Name Insert Function Antibiotic Resistance Activity PI 52962 lentiCas9-Blast Sp Cas9 Cut...codon-optimized S. pyogenes Cas9 protein and blasticidin resistance from EFS promoter. Lentiviral backbone. Zhang...transcriptional activator . ID Name Insert Antibiotic Resistance Activity PI 61422 dCAS9-VP64_GFP dCAS9 (D10A,...lenti vector encoding dCAS9-VP64 with 2A Blast resistance marker (EF1a-NLS-dCas9(N863)-VP64-2A-Blast-WPRE...HSF1 activator helper complex with a 2A Hygro resistance marker (EF1a-MS2-p65-HSF1-2A-Hygro-WPRE) Zhang...to combinatorially index cells for single-cell analysis of clonal dynamics. This library expresses EGFP...

...promotes protein and lipid synthesis, and increases energy production through glycolysis to fuel these energy-intensive...key metabolic regulator, correlates with poor prognosis in many cancers. Cancer...Ras Pathway p53 Pathway Tackling Cancers’ Drug Resistance with a New Screening Kit Background mTOR Pathway...catabolic processes including autophagy and lysosome biogenesis. mTORC2 does not respond to nutrients, but it... either mTORC1 or mTORC2 correlates with poor prognosis in many cancers, including breast, colon, ovarian...protein S6 kinase B1 TSC1/2 TSC1 TSC2 Tuberous sclerosis VHL Von Hippel-Lindau tumor suppressor Return ... Kit (Sabatini & Wood) Tackling Cancers’ Drug Resistance with a New Screening Kit (from David Sabatini...

...including resistances to chemotherapy drugs and toxins, cell viability, and tumor metastasis. One of the...editing systems include targeted AID-mediated mutagenesis (TAM) and CRISPR-X , in which Cas9 is fused to...nsertion of T ransposable E lements by G uide R NA- A ssisted T argeting), developed by Samuel H. Sternberg’s...large scale edits. PASSIGE ( P rime- E diting- A ssisted S ite- S pecific I ntegrase G ene E diting), developed...desired phenotype. The ease of gRNA design and synthesis, as well as the ability to target almost any genomic...simplest dCas9-based activators and repressors consist of dCas9 fused directly to a single transcriptional...than other activators/repressors due to their persistence and heritability. Not all modifications are stable...

...'s genome, which can lead to oncogenesis (termed insertional mutagenesis). Read our γ-Retrovirus Guide...cells; additional plasmids include Puro resistance and Neo resistance Weinberg 10676 pMKO.1 GFP MoMLV U6-driven...immortalized cells (see Weinberg Lab page for resistance variants) Weinberg 1773 pBABE-hygro-hTERT MoMLV... also expresses GFP. See pMKO.1 puro for Puro resistance. Hahn 63704 pRetroX GFP T2A Cre CMV/MSV Tetracycline...

...shRNA empty plasmid with puro resistance. See plasmid 24150 for hygro resistance. Weinberg 10878 pLKO.1 – ... genome, it has the potential for insertional mutagensis. The 2nd and 3rd generation lentiviral systems...fusion; can be used for cDNA expression; puro resistance Sabatini 14883 FUGW 3rd hUbC-driven EGFP; can...expression, gateway plasmid, constitutive Hygromycin resistance gene coexpression. See article for other versions...neomycin selection. See plasmid 21915 for Puro resistance. Wiederschain 36084 pLV-mCherry 3rd CMV driven...

...transcriptional activation of endogenous genes. It consists of three components: A nucleolytically inactive...lentiviral vector encoding dCAS9-VP64 with 2A Blast resistance marker 61426 : lentiviral vector encoding the...HSF1 activator helper complex with a 2A Hygro resistance marker 61427 : lentiviral sgRNA cloning backbone...loops at tetraloop and stemloop 2 and EF1a-zeo resistance marker. Contains BsmBI sites for insertion of...enables Cre/loxP recombination and fluorescence assisted sorting of cells and nuclei in addition to sgRNA...facilitates Cre/loxP recombination and fluorescence assisted sorting of cells and nuclei in addition to sgRNA...

...182 Mycobacterium tuberculosis CRISPRi Library (RLC12) 163954 Inhibition M. tuberculosis Rock NA Varies ...virtually any genetic locus. CRISPR pooled libraries consist of thousands of plasmids, each containing multiple...Fly Yeast T. gondii E. coli S. pneumoniae M. tuberculosis M. smegmatis Green monkey ( C. sabaeus ) Kaposi's...Inhibition Human Weissman 3rd 10 Varies CRISPR/Cas9-assisted Removal of Mitochondrial DNA (CARM) Library 82480...169082 Knockout Mouse Turner N/A 10 13,500 Mouse Metastasis CRISPR-Cpf1(Cas12a) Double Knockout Library 123361...

...developmental biology. With its rapid development (embryogenesis is complete in ~3 days), short generation time...Keith Joung Lab CRISPR/Cas9-based conditional mutagenesis in zebrafish - Wenbiao Chen Lab A CRISPR/Cas9...zebrafish - Bret Pearson Lab MAZe: a tool for mosaic analysis of gene function in zebrafish - Julian Lewis Lab... loss and ablation of previously resistant cell types. CreLite - George Eisenhoffer Lab. ...Neurodegenerative Disorders - Nicholas Cole Lab. A kit consisting of plasmids with four promoters, six fluorophores...

... ). The phosphorylation sites (“phosphosites”) consist of a central phosphoserine site flanked by 15 amino...ambiguity. Rinehart Lab Improved Phosphoprotein Synthesis Reagents Type ID Item Strain 192872 rEcoli XpS...lactamase S68TAG Plasmid 69118 E17TAG GFP zeo resistance Strain 34929 BL21ΔserB Strain 52055 EcAR7 Plasmid...Recombinant Phosphoproteins Using Cell-Free Protein Synthesis. Oza JP, Aerni HR, Pirman NL, Rogulina S, ter ...phosphoserine insertion during Escherichia coli protein synthesis via partial UAG codon reassignment and release...

...RCIscript-GoldyTALEN is designed for in vitro synthesis of TALEN mRNAs. Both 5’ and 3’ Xenopus β-globin...custom repress gene expression in yeast. TALORs consist of the DNA-binding domain of a TALE, along with... promoter or can be achieved by in vitro mRNA synthesis from the T7 promoter. Truncations were introduced...pCAGGS-TAL-NC2, are mammalian expression/mRNA synthesis vectors containing unique shorter N- and C-terminal... has successfully been employed for deletion mutagenesis and gene targeting in human and mouse pluripotent...

...understanding of COVID-19 and related coronaviruses. To assist with this effort Addgene will maintain this plasmid... more are currently undergoing quality control analysis in our lab and will be made available as soon ... a cysteine endosomal protease that triggers proteolysis of SARS-CoV Spike protein, activating its membrane...Spike Receptor Binding Domain Site-Saturation Mutagenesis Libraries - Libraries of variant Spike protein...and ADAM17 cleave ACE2 differentially and only proteolysis by TMPRSS2 augments entry driven by the severe...