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We narrowed to 618 results for: abo.2

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Showing: 21 - 40 of 618 results

  1. Antibody Plasmid Collection

    Type
    Collection
    ...phage display. J Immunol Methods. 2000 Aug 28;242(1-2):159-81. Carlos Barbas Vector system for expression...+J phagemid libraries. Immunol Lett. 2015 Oct;167(2):95-102. Joanna Bereta Sybody Generation Toolbox for... system, fused to sfGFP for imaging. Learn more about antibodies and their applications in our Antibody...phage display vector system for isolation of scFv, diabody and Fab fragment libraries from chickens Methods...

  2. CRISPR Guide

    Type
    Collection
    ...Browse Plasmids: Double-Strand Break (Cut) Figure 2: Overview of the NHEJ repair mechanism. Multiplex ...systems enable researchers to target anywhere from 2–7 genetic loci by cloning multiple gRNAs into a single...Cas9 is included in the gRNA-containing plasmid, or 2-vector systems, in which Cas9 must be delivered separately...your experimental cell population (Figure 8E). In a 2-vector system, you’ll need to either co-infect with...the presence of infectious organisms (like SARS-CoV-2 ) and genetic mutations. Similar to Cas9 and Cas12...Cas enzymes? CasPEDIA is an encyclopedia of Class 2 CRISPR systems with wiki entries describing enzyme...off-target effects by using a single Cas9 nickase and 2 different gRNAs, which bind in close proximity on ...

  3. Plan Your Experiment

    Type
    Collection
    ...components (Figure 2), and the optimal system will likely depend on your cell type. Figure 2: Different delivery...either (1) a lack of gRNA and/or Cas9 expression or (2) a lack of efficient target cleavage in cells that...CRISPR-Based genome editing. Trends in Biotechnology , 36 (2), 173–185. https://doi.org/10.1016/j.tibtech.2017.11.006...exon coding for an essential protein domain could abolish protein activity and essentially function as a ...reference sequence you used for gRNA design. Read more about how to design your gRNA . Synthesize and Clone Desired... other downstream methods. For more information about viral vectors and their production, see our viral...and Proteins In addition to the methods described above, you can also directly deliver mature mRNA or purified...

  4. Antibody Guide

    Type
    Collection
    ...peptide linkers. Diabodies can be used for assembling protein nanostructures. Figure 2: An antibody (IgG...cells; function is unknown Monomer with a valency of 2 IgE Protects against parasites and is responsible ...such as anaphylactic shock Monomer with a valency of 2 IgG The most abundant isotype in blood, also found...secondary immune response Monomer with a valency of 2 IgG1; IgG2; IgG3; IgG4 IgM Produced during the primary... ranging from antibody fragments (shown in Figure 2) to completely unrelated molecules. Examples include...are often used in clinical applications. Diabodies - Diabodies contain two Fab fragments, each recognizing...cells isolated from animals immunized as described above are used to create hybridomas, which produce large...

  5. AAV Viral Preps

    Type
    Collection
    ...AAV vectors for trial purposes in serotypes AAV1, 2, 5, 6, 8, 9, 11, and retrograde Retrograde AAV To ...it will be listed on the Function section pages above. You can also do a search of viral preps and filter...

  6. Bikard Lab - CRISPR Repression Collection

    Type
    Collection
    ...chromosomal integration of DNA. ACS Synthetic Biology, 2(9), 537–541. DOI: 10.1021/sb400021j Figure 1: Multiplexing...attachment site of the phage 186. Detailed information about the repression system can be found in this article...29519933 . bioRxiv preprint . Detailed information about chromosomal integration of pLC97 can be found in...

  7. Optogenetics Plasmid Collection

    Type
    Collection
    ...optogenetic tool is the widely used Channelrhodopsin-2 (ChR2) from Chlamydomonas reinhardtii . ChR2 is a ...'s Optogenetics Guide . Interested in more news about optogenetics research? Find related posts on Addgene's...

  8. CRISPR Plasmids - Cascade-Cas3

    Type
    Collection
    ...are largely underutilized compared to their Class 2 counterparts like Cas9. This is attributed in part...tables below for CRISPR Cas3 plasmids. To learn more about Cas3 and other CRISPR topics, read our CRISPR Guide...

  9. CRISPR Plasmids - Tagging

    Type
    Collection
    ...gene-specific CRISPR and donor vectors as well as pX330S-2-PITCh, expressing a generic gRNA targeting the PITCh...Protocols, 2016 . Yamamoto Tagging Plasmids: pX330S-2-PITCh - expresses Cas9 & PITCh-gRNA pX330A-FBL/PITCh.... Read our CRISPR 101 post for more information about HDR/repair template design. How to use CRISPR to...

  10. Resolute Plasmid Collection

    Type
    Collection
    ... funding from the Innovative Medicines Initiative 2 Joint Undertaking under grant agreement No 777372....Representation of RESOLUTE mission. The combination of metabolomics and genome engineering forms the core of the...

  11. Addgene Packaged on Request: Scope of Service

    Type
    Collection
    ...AAV6, AAV8, AAV11, or AAV Retrograde serotypes and 2 x 10 12 GC/mL for the AAV2 serotype, as titered by... by email will supersede the general guarantees above. We will have our expert viral vector production...supersede the general estimated turnaround time above. After starting viral production, if we determine...

  12. CRISPR References and Information

    Type
    Collection
    ...Link opens in a new window) An encyclopedia of Class 2 CRISPR systems with wiki entries describing enzyme...library amplification 1 vector system: lentiCRISPR v2 2 vector system: lentiCas9-Blast and lentiGuide-Puro...screening. CRISPR eBook Addgene's downloadable eBook about all things CRISPR. Addgene CRISPR blogs CRISPR tips...Developed by the Fredrik Wermeling Lab . Read more about it on the Addgene blog . CRISPOR (Link opens in ... Lab and maintained by the Frederick National Laboratory at the National Cancer Institute. Cas-OFFinder...maintained by the Jennifer Doudna Lab . Read more about it on the Addgene blog . CRISPRflydesign (Link opens...

  13. E11 Bio PRISM Collection

    Type
    Collection
    ...optical imaging down to individual synapses. Figure 2: PRISM workflow. A) Stochastic infection of pooled...Learn about PRISM, a set of molecular tools for high-throughput reconstruction of individual neurons ... Research (Link opens in a new window) , in collaboration with The Francis Crick Institute (Link opens...Link opens in a new window) . For more information about PRISM, see the preprint: Park, S.Y., Sheridan, A...

  14. Institute for Protein Innovation

    Type
    Collection
    ... proteins are currently under development. Figure 2: Key adhesion proteins and receptor–ligand pairs bridge...of cell surface and secreted proteins from a collaboration between Addgene and IPI. Antibodies...antibodies in an open science model. Learn more about IPI (Link opens in a new window) Axon Guidance IPI...

  15. Antibody Production

    Type
    Collection
    ...for shipping stability by incubating at 37 °C for 2 weeks, freezing at -80 °C, thawing on ice, and then...quality control partner before distribution. Details about our production protocols, titering methods, and ...Depositing Lab on the antibody webpage. To learn more about these antibodies, please visit the Institute for...

  16. Distribution to Industry

    Type
    Collection
    ...and HaloTag Fusions from Promega COVID-19 SARS-COV-2, ACE2, and CRISPR tools Fluorescent Proteins from ...plasmids that are available to industry. Learn more about the available plasmids and how to order them. Distribution...Information page for more information. To learn more about Addgene's MTA process please visit our Help Center...

  17. New England Biolabs Cell-Imaging Plasmid Collection

    Type
    Collection
    ...transient mammalian expression 101126 pACP-tag (m)- 2 Vector ACP/MCP-tag Empty backbone for stable and transient...available Non-toxic to living cells To learn more about this system, visit the New England Biolabs website...

  18. Luciferase Plasmid Collection

    Type
    Collection
    ...transfection Feng Zhang 21471 pLenti PGK V5-LUC Neo (w623-2) Firefly PGK Lentiviral expression of firefly luciferase...fusing NanoLuc® and Venus fluorophore to Troponin C Ca 2+ binding domain. Luminopsins : Luciferase-opsin fusion... vivo imaging or creating biosensors. Read more about BRET on our blog . Nano-Lanterns: Fluorescent proteins...

  19. Tetracycline Inducible Expression

    Type
    Collection
    ...hSyn promoter rtTA3 Wei Xu 26803 pEnt L1L3 EF1a-tTA-2 Gateway entry vector to express tTA from EF1α promoter...expression and improved dynamic range . Hum Gene Ther, 22 (2), 166–176. https://doi.org/10.1089/hum.2010.099 (Link...Learn about tetracycline-regulated gene expression and find plasmids for your experiments using tet- ...systems: Tet-Off and Tet-On. Read on to learn more about the components of tet systems and how to use them...

  20. Brain Initiative Collection

    Type
    Collection
    ...Gordon Fishell 83899-AAV1 pAAV-mDlx-GCaMP6f-Fishell-2 GCaMP6f expression in forebrain GABA-ergic interneurons...Gordon Fishell 83899-AAV9 pAAV-mDlx-GCaMP6f-Fishell-2 GCaMP6f expression in forebrain GABA-ergic interneurons...Gordon Fishell 83899-AAVrg pAAV-mDlx-GCaMP6f-Fishell-2 GCaMP6f expression in forebrain GABA-ergic interneurons...particles that are suitable for in vivo use. Learn more about our viral service ID Viral Prep Description PI 65417...well as by our trusted partner labs. Learn more about our antibodies ID Recombinant Antibody Reactivity...
Showing: 21 - 40 of 618 results