We narrowed to 560 results for: cat.3

...development (embryogenesis is complete in ~3 days), short generation time (~3 months), ease of care and breeding...Society (IZFS) - An organization that promotes and advocates for zebrafish research in the international research...

...(exo-) DNA Polymerase I, Large (Klenow) Fragment (3'-5' exo-) 165512 pOpen-THEAQpolA Taq DNA Polymerase...MMLV) Reverse Transcriptase (RNAse H deactivated by 3 mutations) 165556 pOpen-MMLV_RT (lack H) Moloney Murine...polymerase chain reaction (PCR), cloning, isothermal amplification, reverse transcription of RNA into DNA, and ...for fixing damaged and degraded DNA prior to amplification, enzymes for cutting protein sequences to remove...then transform into E. coli for expression and purification ( pET28a-LIC or other pET vectors are recommended...

... that titers obtained using ddPCR are generally 2–3 fold higher than those achieved using the standard... appear darker than full vector particles. Figure 3: Electron micrograph of AAV vector preparation shows...minimized by using an endotoxin-free plasmid purification protocol. To minimize the immunogenic properties...posted on the material page for the corresponding catalog item (see maps section for images). Figure 2: AAV...typically prepared using endotoxin-free plasmid purification kits. Resources Browse lentiviral preps available...

...for in vitro synthesis of TALEN mRNAs. Both 5’ and 3’ Xenopus β-globin UTRs are included in the vector ...Gate TALEN Kit and are used in place of pTAL1, 2, 3, or 4. For both plasmids sequence positions 1214–2210...Golden Gate TALEN kit. The GoldyTALEN scaffold is truncated at both the N and C terminus and induces mutation...pC-GoldyTALEN directs expression of TALENs from a truncated CAGs promoter. RCIscript-GoldyTALEN is designed...in vitro mRNA synthesis from the T7 promoter. Truncations were introduced to the N- and C-terminus of the...Golden Gate TALEN 2.0 48705 pTAL7a Boris Greber Application of TALEN technology in hPSCs pTAL7a and pTAL7b...expression in mammalian cells, (iv) an improved, truncated TALE backbone architecture as established by Miller...

...N1 - Mammalian Expression miRFP670 642 670 12 4.5 3 h Monomer pmiRFP670-N1 - Mammalian Expression pLenti6.2... Expression mKeima (pH sensitive) 440 620 3.5 6.5 3 h Monomer pFA6a-link-yomKeima-CaURA3 - Yeast Expression...- Bacterial Expression PAiRFP1 690 717 660 or 750 3 Dimer pPAiRFP1-N1 - Mammalian Expression pBAD/HisB-PAiRFP1...- Bacterial Expression PAiRFP2 692 719 660 or 750 3 Dimer pPAiRFP2-N1 - Mammalian Expression pBAD/HisB-PAiRFP2...rsEGFP1 493 510 405 (Off to On) 491 (On to Off) 17 6.5 3 h rsGFP1-pBAD - Bacterial Expression Skylan-NS 499...and emission maxima (Stokes shift), useful for applications like multicolor imaging with better spectral...

... and breathing, and often results in death within 3–5 years from symptom onset. Disease mechanisms are...et al. Dev Cell. 2024) Image exocytosis with VAMP2/3 plasmids to study CNS myelination. (Lam et al. Nat...loci seem to be associated with the disease and implicate a wide range of cellular processes. The vast majority... consists mainly of managing symptoms through medication and surgery. PD primarily involves the malfunction...available from Addgene, sortable by type of genetic modification and target species. You can also find our guide...synaptic transmission with improved glutamate indicators . (Aggarwal et al. bioRxiv. 2025) Monitor dopaminergic...window) A news website and information resource dedicated to helping researchers accelerate discovery and...

... consists of 5 individually packaged plasmids and 3 individually packaged strains, which are shipped as...Protocols & Resources Contents of Kit Original Publication Oligomerized pool engineering (OPEN): an 'open-source... in the Materials and Methods of your future publications. For your Materials and Methods section: "The...The plasmid kit used in this publication was a gift from Keith Joung (Addgene kit # 1000000013)" For your...

...Transfer plasmids with sequence from the 5′ to the 3′ ITR exceeding 4.7 kb Transfer plasmids with a cargo...without a guaranteed titer. Any titer expectations communicated by email will supersede the general guarantees...step of the QC process, viral genome sequence verification, may not yet have been completed at the time...longer turnaround time. Any timing expectations communicated by email will supersede the general estimated...

...). DeChenne-Peters et al. PLoS One. 2023 Mar 9;18(3):e0282170. PMID: 36893201 (Link opens in a new window...dehydrogenase (MDH). The MCC is a national group of educational leaders providing research opportunities to their...other members of the MCC community. They support educators interested in integrating MDH-related research...professional development, membership to a local hub of educators teaching MDH CUREs, and opportunities to mentor...of action, regulation and post-translational modifications, and metabolic pathways and protein interactions...His-tags are cloned at the C-terminus of MDH and, if indicated on the plasmid map, a TEV cleavage site is present...access for biochemistry research in undergraduate education: The malate dehydrogenase CURE community. J Biol...

...conditional systems for RNA interference . Nature Methods, 3 (9), 682–688. PubMed (Link opens in a new window) ...search our full site . ID Plasmid Vector Type PI Publication Additional Resources Addgene Resources pLKO.1...

...is able to replicate in the starter cell using the G-protein, which is supplied in trans . (3) Nascent ...which is not required for the transcription or replication of the genome within infected cells, but is required... cells that lack the G protein, the virus can replicate but the newly formed viruses are unable to infect...offers you even more options. Select from a vast catalog of eligible AAV plasmids and we’ll make the viral...

...blocking ligand binding and receptor function Figure 3: IPI Integrin antibodies exhibit distinct binding ...Antibody Description Isotype Reactivity Recommended Applications PI Browse Glypican Antibodies Glypicans are ...Antibody Description Isotype Reactivity Recommended Applications PI Return to top Synaptic Cleft IPI’s Synaptic...Antibody Description Isotype Reactivity Recommended Applications PI Return to top Integrins Integrins are a family...Antibody Description Isotype Reactivity Recommended Applications Return to top Browse Epitope Tag Antibodies ...Antibody Collection page to browse Addgene’s full catalog of antibodies that target epitope tags and other...Antibody Description Isotype Reactivity Recommended Applications Return to top Browse Antibody Plasmids The following...

... work in your mouse line, see Supplementary Table 3 (Link opens in a new window) . Browse Available PHP.eB...Inhibitory - AAV-PHP.eB with GRE or transgenic animal L2/3 & L4 - AAV.CAP-B10 with GRE or transgenic animal Excitatory...PHP.eB AAV ID Name Promoter Description Category PI Controls 28306 pAAV-FLEX-tdTomato CAG tdTomato, Cre-dependent...Available PHP.S AAV ID Name Promoter Description Category PI 28306 pAAV-FLEX-tdTomato CAG tdTomato, Cre-...Available PHP.V1 AAV ID Name Promoter Description Category PI 104052 pAAV-CAG-DIO-EYFP CAG EYFP, Cre-dependent...Available MaCPNS1 AAV ID Name Promoter Description Category PI 37825 pAAV-CAG-GFP CAG GFP Control Boyden MaCPNS2...Available MaCPNS2 AAV ID Name Promoter Description Category PI 37825 pAAV-CAG-GFP CAG GFP Control Boyden CAP-B10...

...genetic model organism due to their rapid life cycle (3 days from egg to adult) and easy lab maintenance. ...Efficient Marker-Free Recovery of Custom Genetic Modifications with CRISPR/Cas9 in Caenorhabditis elegans. ...consortium of biologists and computer scientists dedicated to providing the research community with accurate...

...Fishell 83898-AAV1 pAAV-mDlx-ChR2-mCherry-Fishell-3 ChR2-mCherry fusion expression in forebrain GABA-ergic...Fishell 83898-AAV9 pAAV-mDlx-ChR2-mCherry-Fishell-3 ChR2-mCherry fusion expression in forebrain GABA-ergic...Fishell 83898-AAVrg pAAV-mDlx-ChR2-mCherry-Fishell-3 ChR2-mCherry fusion expression in forebrain GABA-ergic... the human brain through the development and application of innovative tools enabling large-scale real-time...or when BRAIN Initiative grants are noted in publications associated with Addgene materials. Plasmids ...Gradinaru 105448-AAV9 pAAV-hSyn-DIO-ChrimsonR-mRuby2-ST Cation channelrhodopsin ChrimsonR fused to mRuby2 fluorophore...108912-AAV9 pAAV-CAG-DIO-ChroME-ST-P2A-H2B-mRuby3 Cation channelrhodopsin ChroME targeted to the neuronal...

...Doxycycline-inducible Gene Expression . Curr Gene Ther, 16 (3), 156–167. https://doi.org/10.2174/1566523216666160524144041...and E with an inducible system . Mol Cell Biol, 14 (3), 1669–1679. https://doi.org/10.1128/mcb.14.3.1669... Return to Top Other Tet Applications Explore some other popular applications of tetracycline systems ...for comparison of Tet-On systems in different applications. Use of Tetracycline or a Derivative Doxycycline...vectors for transactivators, and vectors for other applications, or search our collection for all dox-regulated... Tet-On 3G transactivator Tet-On 3G rtTA Elena Cattaneo 104543 PB-EF1a-TetOn3G PiggyBac vector expressing...-tag from TRE3G promoter for Tandem-Affinity Purification. Yannick Doyon 20321 TetO-FUW-OSKM Tet-On inducible...

...a template. In general, edits must be downstream (3’) of the nick site, and guides that target closer ...control of gene repression and activation. Cell , 159 (3), 647–661. https://doi.org/10.1016/j.cell.2014.09.029...Achieving your desired edit comes down to location, location, location. Positioning your chosen Cas enzyme ...designing the gRNA component for each category. Genetic Manipulation Application Cas Enzyme Additional Considerations...the table below, we highlight the most common categories of genetic manipulations used in mammalian cells...gene you target will depend on your specific application. Knockout : Target constitutively expressed regions...start sites that could allow for expression of a truncated protein. Targeting an exon coding for an essential...

...observed in the absence of genome editing (Fig. 2 and 3) (5, 3). Figure 2: Gene activation with nuclease-competent... Figure 3: Gene repression with nuclease-competent Cas9. When complexed with a truncated gRNA, Cas9...its nucleolytic activity is eliminated (Fig. 1) (1, 3). The gRNA can be further modified by incorporation...methods 12.9 (2015): 823-826. PubMed PMID: 26167643. 3. Kiani, Samira, et al. "Cas9 gRNA engineering for ...target site. Benefits of truncated gRNAs Importantly, the use of truncated guides has largely been demonstrated...protospacer, in the genome. Native Cas9 has two catalytic domains, each of which cleaves one strand of DNA...gRNA (20nt complementarity to target site) or truncated gRNA (≤15nt complementarity to target site). When...

...found climbing mountains or playing with his two cats. Keira Havens grew up in Hawaii where she was fascinated...genetic tools facilitates plant science. Challenge #3: Intellectual property There is another challenge ...biotechnologists face is public perception of genetic modification (GM) and synthetic biology. The mistrust and...cannot build what we do not understand, and the complicated and unique metabolic processes of plants are ...

...shRNA cassettes into the unique SnaBI site in the 3´-LTR). Find more pULTRA versions . Malcolm Moore 25895... plasmids to express a CRISPR gRNA? Browse our catalog for lentiviral CRISPR plasmids for gRNA expression...monitor expression. Luk Parijs 11619 pLB 3rd Modification of pLL3.7 with added genetic elements known ...3rd For barcoding transcription factors for identification in pooled screens. Part of the MORF Collection...