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We narrowed to 563 results for: cat.3

Showing: 21 - 40 of 563 results

  1. Zinc Finger Consortium: Zinc Finger Arrays

    Type
    Collection
    ...Arrays Engineering Method Full Target Site (5' to 3') Left half-site Right half-site CACNA1F OZ507 and...nucleases. Foley JE et al. Nat Protoc. . 2009. Dec 3. 4(12):1855-1867. PubMed PMID 20010934 ....OPEN gAGCATCACCtcgctGGAGGTGAGc PTRF/Cavin1 OZ513 and OZ514 OPEN OPEN gACAGCCGTCtgcatctGGAGGTGCCc pitrm1...OPEN OPEN aGCCATCATCaggttcaGGATGAGCCc Kif6 OZ537 and OZ538 OPEN OPEN aAGCTTCCTCttcattgGCTGACTGCa ldr (low...CODA OPEN cAACCTCAGCATAAAGCTGGAGGAg psen2 OZ577 and OZ578 CODA OPEN cGGCATCTCCAGAGCCCGAGGCGGACc zgc:162148...hydroxylase (dbh) OZ525 and OZ526 OPEN OPEN tCCCATCCTAtcgttGCATGAGCTc mdka (midkine-related growth factor) OZ527...lipoprotein receptor) OZ539 and OZ540 OPEN OPEN tTGCATCCACggcagTCGGCAGTGc Encephalopsin (Opn3) OZ541 and OZ542 OPEN...

  2. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ...inducible (Tet) expression miRNA and 3' UTR mRNA regulation pIS0 - 3'UTR segments of target genes can be... for gene expression Thy1.1 Mammalian pLKO.3 Thy1.1 - Lentiviral shRNA expression Gentamicin Varies...see more Gateway vectors) pCAP03-acc(3)IV - Facilitate the capture and expression of bacterial...plasmids containing only the elements needed for replication in the host cell and are designed for a user ...Protein Function: Viral Vector Delivery, Genome Modification, mRNA Regulation, and More Selectable Markers...collection page Zebrafish CMV, h2afv, XlEef1a1 See our dedicated Zebrafish Plasmids and Resources collection page...Additionally, tags are commonly added to aid in protein purification. Just remember to remove the stop codon for ...

  3. Zebrafish Plasmid Collection

    Type
    Collection
    ...development (embryogenesis is complete in ~3 days), short generation time (~3 months), ease of care and breeding...Society (IZFS) - An organization that promotes and advocates for zebrafish research in the international research...

  4. Open Enzyme Collection

    Type
    Collection
    ...(exo-) DNA Polymerase I, Large (Klenow) Fragment (3'-5' exo-) 165512 pOpen-THEAQpolA Taq DNA Polymerase...MMLV) Reverse Transcriptase (RNAse H deactivated by 3 mutations) 165556 pOpen-MMLV_RT (lack H) Moloney Murine...polymerase chain reaction (PCR), cloning, isothermal amplification, reverse transcription of RNA into DNA, and ...for fixing damaged and degraded DNA prior to amplification, enzymes for cutting protein sequences to remove...then transform into E. coli for expression and purification ( pET28a-LIC or other pET vectors are recommended...

  5. Viral Production

    Type
    Collection
    ... that titers obtained using ddPCR are generally 2–3 fold higher than those achieved using the standard... appear darker than full vector particles. Figure 3: Electron micrograph of AAV vector preparation shows...minimized by using an endotoxin-free plasmid purification protocol. To minimize the immunogenic properties...posted on the material page for the corresponding catalog item (see maps section for images). Figure 2: AAV...typically prepared using endotoxin-free plasmid purification kits. Resources Browse lentiviral preps available...

  6. TALEN Plasmids and Kits

    Type
    Collection
    ...for in vitro synthesis of TALEN mRNAs. Both 5’ and 3’ Xenopus β-globin UTRs are included in the vector ...Gate TALEN Kit and are used in place of pTAL1, 2, 3, or 4. For both plasmids sequence positions 1214–2210...Golden Gate TALEN kit. The GoldyTALEN scaffold is truncated at both the N and C terminus and induces mutation...pC-GoldyTALEN directs expression of TALENs from a truncated CAGs promoter. RCIscript-GoldyTALEN is designed...in vitro mRNA synthesis from the T7 promoter. Truncations were introduced to the N- and C-terminus of the...Golden Gate TALEN 2.0 48705 pTAL7a Boris Greber Application of TALEN technology in hPSCs pTAL7a and pTAL7b...expression in mammalian cells, (iv) an improved, truncated TALE backbone architecture as established by Miller...

  7. Fluorescent Protein Guide: Empty Backbones

    Type
    Collection
    ...N1 - Mammalian Expression miRFP670 642 670 12 4.5 3 h Monomer pmiRFP670-N1 - Mammalian Expression pLenti6.2... Expression mKeima (pH sensitive) 440 620 3.5 6.5 3 h Monomer pFA6a-link-yomKeima-CaURA3 - Yeast Expression...- Bacterial Expression PAiRFP1 690 717 660 or 750 3 Dimer pPAiRFP1-N1 - Mammalian Expression pBAD/HisB-PAiRFP1...- Bacterial Expression PAiRFP2 692 719 660 or 750 3 Dimer pPAiRFP2-N1 - Mammalian Expression pBAD/HisB-PAiRFP2...rsEGFP1 493 510 405 (Off to On) 491 (On to Off) 17 6.5 3 h rsGFP1-pBAD - Bacterial Expression Dronpa3 490 515...and emission maxima (Stokes shift), useful for applications like multicolor imaging with better spectral...

  8. CRISPR Plasmids - gRNAs

    Type
    Collection
    ...that are upstream of a 5' NGG 3' PAM sequence. Which CRISPR application is this gRNA sequence compatible...RNA Targeting RNA Targeting RNA Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled...using any of these gRNA plasmids and review the publication associated with each plasmid for more information...CRISPR nuclease and function. Please see the publication or plasmid information page, or contact the depositing...plasmids. ID Plasmid Gene/Insert Vector Type PI Publication Do you have suggestions for other plasmids that...

  9. Zinc Finger Consortium: OPEN Reagents

    Type
    Collection
    ... consists of 5 individually packaged plasmids and 3 individually packaged strains, which are shipped as...Protocols & Resources Contents of Kit Original Publication Oligomerized pool engineering (OPEN): an 'open-source... in the Materials and Methods of your future publications. For your Materials and Methods section: "The...The plasmid kit used in this publication was a gift from Keith Joung (Addgene kit # 1000000013)" For your...

  10. CRISPR Plasmids - C. elegans

    Type
    Collection
    ...species = S. pyogenes (PAM = NGG) 47549 pDD162 (Peft-3::Cas9 + Empty sgRNA) R07E5.16 U6 yes, cut Goldstein...ID Plasmid Gene/Insert Promoter PI Publication Activate Catalytically dead dCas9 fused to a transcriptional...RNA Targeting RNA Targeting RNA Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled...introduce a double-strand break (DSB) at a specific location based on a gRNA-defined target sequence. DSBs ...specific locus. ID Plasmid Gene/Insert Promoter PI Publication Empty gRNA Expression Vectors Select a gRNA expression...but their function is often limited to a single category (cut, nick, etc.) On the other hand, gRNA plasmids...

  11. Addgene Packaged on Request: Scope of Service

    Type
    Collection
    ...Transfer plasmids with sequence from the 5′ to the 3′ ITR exceeding 4.7 kb Transfer plasmids with a cargo...without a guaranteed titer. Any titer expectations communicated by email will supersede the general guarantees...step of the QC process, viral genome sequence verification, may not yet have been completed at the time...longer turnaround time. Any timing expectations communicated by email will supersede the general estimated...

  12. Malate Dehydrogenase CUREs Community Collection

    Type
    Collection
    ...). DeChenne-Peters et al. PLoS One. 2023 Mar 9;18(3):e0282170. PMID: 36893201 (Link opens in a new window...dehydrogenase (MDH). The MCC is a national group of educational leaders providing research opportunities to their...other members of the MCC community. They support educators interested in integrating MDH-related research...professional development, membership to a local hub of educators teaching MDH CUREs, and opportunities to mentor...of action, regulation and post-translational modifications, and metabolic pathways and protein interactions...His-tags are cloned at the C-terminus of MDH and, if indicated on the plasmid map, a TEV cleavage site is present...access for biochemistry research in undergraduate education: The malate dehydrogenase CURE community. J Biol...

  13. CRISPR Plasmids - Empty gRNA Vectors

    Type
    Collection
    ...Bacteria SpeI + HindIII none S. pyogenes Qi pDD162 (Peft-3::Cas9 + Empty sgRNA) 47549 C. elegans yes, cut S. ... Puro Abbosh pCFD3-MS2tail-Scaffold 78906 Fly dU6:3 none S. pyogenes Virmilion Perrimon pHDE-35S-Cas9-...polycistronic lentiviruses. Can express Cas9 with up to 3 gRNAs. Frew pSQT1313 Mammalian Single plasmid for ...expression of two gRNAs from Drosophila U6:1 and U6:3 promoters. Bullock pCFD5 Drosophila Utilizes endogenous...RNA Targeting RNA Targeting RNA Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled...

  14. Mammalian RNAi Tools

    Type
    Collection
    ...conditional systems for RNA interference . Nature Methods, 3 (9), 682–688. PubMed (Link opens in a new window) ...search our full site . ID Plasmid Vector Type PI Publication Additional Resources Addgene Resources pLKO.1...

  15. AAV for Neuronal Tracing

    Type
    Collection
    ...is able to replicate in the starter cell using the G-protein, which is supplied in trans . (3) Nascent ...which is not required for the transcription or replication of the genome within infected cells, but is required... cells that lack the G protein, the virus can replicate but the newly formed viruses are unable to infect...offers you even more options. Select from a vast catalog of eligible AAV plasmids and we’ll make the viral...

  16. Caltech Systemic Capsids

    Type
    Collection
    ... work in your mouse line, see Supplementary Table 3 (Link opens in a new window) . Browse Available PHP.eB...Inhibitory - AAV-PHP.eB with GRE or transgenic animal L2/3 & L4 - AAV.CAP-B10 with GRE or transgenic animal Excitatory...PHP.eB AAV ID Name Promoter Description Category PI Controls 28306 pAAV-FLEX-tdTomato CAG tdTomato, Cre-dependent...Available PHP.S AAV ID Name Promoter Description Category PI 28306 pAAV-FLEX-tdTomato CAG tdTomato, Cre-...Available PHP.V1 AAV ID Name Promoter Description Category PI 104052 pAAV-CAG-DIO-EYFP CAG EYFP, Cre-dependent...Available MaCPNS1 AAV ID Name Promoter Description Category PI 37825 pAAV-CAG-GFP CAG GFP Control Boyden MaCPNS2...Available MaCPNS2 AAV ID Name Promoter Description Category PI 37825 pAAV-CAG-GFP CAG GFP Control Boyden CAP-B10...

  17. Worm Expression Resources

    Type
    Collection
    ...genetic model organism due to their rapid life cycle (3 days from egg to adult) and easy lab maintenance. ...Efficient Marker-Free Recovery of Custom Genetic Modifications with CRISPR/Cas9 in Caenorhabditis elegans. ...consortium of biologists and computer scientists dedicated to providing the research community with accurate...

  18. Brain Initiative Collection

    Type
    Collection
    ...Fishell 83898-AAV1 pAAV-mDlx-ChR2-mCherry-Fishell-3 ChR2-mCherry fusion expression in forebrain GABA-ergic...Fishell 83898-AAV9 pAAV-mDlx-ChR2-mCherry-Fishell-3 ChR2-mCherry fusion expression in forebrain GABA-ergic...Fishell 83898-AAVrg pAAV-mDlx-ChR2-mCherry-Fishell-3 ChR2-mCherry fusion expression in forebrain GABA-ergic... the human brain through the development and application of innovative tools enabling large-scale real-time...or when BRAIN Initiative grants are noted in publications associated with Addgene materials. Plasmids ...Gradinaru 105448-AAV9 pAAV-hSyn-DIO-ChrimsonR-mRuby2-ST Cation channelrhodopsin ChrimsonR fused to mRuby2 fluorophore...108912-AAV9 pAAV-CAG-DIO-ChroME-ST-P2A-H2B-mRuby3 Cation channelrhodopsin ChroME targeted to the neuronal...

  19. Tetracycline Inducible Expression

    Type
    Collection
    ...Doxycycline-inducible Gene Expression . Curr Gene Ther, 16 (3), 156–167. https://doi.org/10.2174/1566523216666160524144041...and E with an inducible system . Mol Cell Biol, 14 (3), 1669–1679. https://doi.org/10.1128/mcb.14.3.1669...tTA-Advanced Takeshi Imai Other Tet Applications Explore some other popular applications of tetracycline systems ...for comparison of Tet-On systems in different applications. Use of Tetracycline or a Derivative Doxycycline...vectors for transactivators, and vectors for other applications, or search our collection for all dox-regulated... Tet-On 3G transactivator Tet-On 3G rtTA Elena Cattaneo 104543 PB-EF1a-TetOn3G PiggyBac vector expressing...-tag from TRE3G promoter for Tandem-Affinity Purification. Yannick Doyon 20321 TetO-FUW-OSKM Tet-On inducible...

  20. Truncated gRNAs for Regulating Gene Expression

    Type
    Blog Post
    ...observed in the absence of genome editing (Fig. 2 and 3) (5, 3). Figure 2: Gene activation with nuclease-competent... Figure 3: Gene repression with nuclease-competent Cas9. When complexed with a truncated gRNA, Cas9...its nucleolytic activity is eliminated (Fig. 1) (1, 3). The gRNA can be further modified by incorporation...methods 12.9 (2015): 823-826. PubMed PMID: 26167643. 3. Kiani, Samira, et al. "Cas9 gRNA engineering for ...target site. Benefits of truncated gRNAs Importantly, the use of truncated guides has largely been demonstrated...protospacer, in the genome. Native Cas9 has two catalytic domains, each of which cleaves one strand of DNA...gRNA (20nt complementarity to target site) or truncated gRNA (≤15nt complementarity to target site). When...
Showing: 21 - 40 of 563 results