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We narrowed to 952 results for: cha;

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Showing: 21 - 40 of 952 results

  1. A new platform for fast functional characterization of potentially cancerous mutations

    Type
    Blog Post
    ...in cancer. Yet, the majority of them remain uncharacterized. This represents a considerable barrier to ...Their work culminated in the first functional characterization of over 1,000 mutations, and the group has...are also sensitivity issues when attempting to characterize mutations in a pooled cell population, as mutations... Michael G. Lemieux...

  2. The Michael Davidson Collection: One-Stop Shop for Fluorescent Proteins

    Type
    Blog Post
    ...plasmids is available in an easily searchable format on Addgene’s Michael Davidson Fluorescent Protein Collection...great work of Michael Davidson and his colleagues." Acknowledgments Thank you to Michael Davidson, Michelle...proteins and their use for imaging cell biology, Michael Davidson’s lab at Florida State University has ... the cell, use these markers as your guide. As Michael Davidson told the Tallahassee Democrat in a story...

  3. Michael Koeris' Journey from Grad Student to Entrepreneur: The Story of Sample 6

    Type
    Blog Post
    ...installment in our podcast series, we chat with new Addgene Board Member, Michael Koeris. Dr. Koeris did his graduate... capital firm, Flagship Ventures, Koeris helped change Novophage's focus. The company went from developing...pathogens in food products. Along the way, the company changed its name from Novophage to Sample 6. To learn ...

  4. Announcing the Winners of the 2021 Michael Davidson and Roger Tsien Commemorative Conference Awards

    Type
    Blog Post
    ...excited to announce the recipients of this year’s Michael Davidson and Roger Tsien Commemorative Conference...conference of their choice while honoring the late Michael Davidson and Roger Tsien who have played enormous...fluorescent protein tools. This year, we’ve made some changes. In addition to supporting in-person conference...health and disease,” says Aggarwal. This biosensor changes fluorescence based on the release of glutamate ...colleagues Filip Tomaska and Tim Hanson, are characterizing variants of iGluSnFR3 in vivo and have used...be transferred to the roGFP2 cysteine thiols and change its fluorescence properties. The biosensor can ...allow us to better understand how neuron activity changes following opioid withdrawal, providing insight ...

  6. Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins

    Type
    Blog Post
    ...Listen to the photoswitchable protein podcast segment Reversible control of photoswitchable proteins, which...the light- and dark- states of the photoswitchable cPYP, which changes in conformation in response to blue...picking approach to select cells with variant characteristics they wanted, the team identified Archon1, a... Discovery of new protein binder tools for photoswitchable proteins cPYP and AsLOV2 Article contributed... which change in protein conformation in response to light, offers a way to spatially and temporally control...structural knowledge of the proteins. To overcome this challenge, the Uppalapati and Woolley lab developed a phage...bind to only the light- or dark- state of the photoswitchable proteins offers a powerful approach to control...

  7. Trimmer Lab NeuroMab Collection

    Type
    Collection
    ...Anti-Pan-Nav1 Na+ channel [N97A/31R] Pan-Nav1 Na+ channel Human Mouse IgG2a 114553 Anti-Pan-Nav1 Na+ channel [N230...Anti-Kv2.1 K+ Channel [D3/71R] Kv2.1 K+ Channel Rat Mouse IgG2a 128613 Anti-Kv1.4 K+ Channel [K13/31R] Kv1.4... Kv1.4 K+ Channel Rat Mouse IgG2a 128614 Anti-Kv1.2 K+ Channel [K14/16R] Kv1.2 K+ Channel Human Mouse ...KCNAB2 K+ Channel [K17/70R] Kvbeta2/KCNAB2 K+ Channel Mouse Mouse IgG2a 128616 Anti-Kv1.1 K+ Channel [K20/....1 K+ Channel Rat Mouse IgG2a 128617 Anti-Pan-Kvbeta K+ Channel [K25/73R] Pan-Kvbeta K+ Channel Mouse ...Anti-Nav1.1 Na+ channel [K74/71R] Nav1.1 Na+ channel Rat Mouse IgG2a 128627 Anti-Kv4.3 K+ Channel [K75/41R] ...] Kv4.3 K+ Channel Rat Mouse IgG2a 128628 Anti-Kv2.1 K+ Channel [K89/34R] Kv2.1 K+ Channel Rat Mouse IgG2a...

  8. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...GAL1 Huntington's Michael Sherman 1385 pYES2/103Q HTT Flag, GFP GAL1 Huntington's Michael Sherman 8412 pMTH...Friedreich ataxia Michael Ristow 14977 pCINeo-hFX(I154F)-HA FXN HA, AU1 CMV Friedreich ataxia Michael Ristow 14978... ataxia Michael Ristow 14979 pGL3-basic-hFX-promotor(1,3kb) FXN hFXN Friedreich ataxia Michael Ristow ...ataxia Michael Ristow 14981 pGL3-basic-hFX-promotor(2kb, distal) FXN hFXN Friedreich ataxia Michael Ristow...Friedreich ataxia Michael Ristow 14988 pBlue-hFX(I154F)-HA FXN HA, AU1 T7 Friedreich ataxia Michael Ristow 14989...Friedreich ataxia Michael Ristow 14990 pBlue-hFX(T133E)-HA FXN HA, AU1 T7 Friedreich ataxia Michael Ristow 14991...ataxia Michael Ristow 14992 pET28(a)-hFX(I154F)-HA FXN HA, HIS, AU1 T7 Friedreich ataxia Michael Ristow...

  9. Genetic Code Expansion

    Type
    Collection
    ...incorporation Michael Jewett 69495 rEc.13.delA 13 amber sites changed to TAA, RF1 function removed Michael Jewett...Methanogenic archaeon Bacterial Ahmed Badran 209739 pAB228i24 G1 PylRS Methanogenic archaeon Bacterial Ahmed... Abhishek Chatterjee 217365 pIDTSmart-CMV-PLRS1 LeuRS E. coli Mammalian Abhishek Chatterjee 218764 pEvoltac-EcW-TGA-h14...TAG sites changes to UAG, RF1 function removed George Church 48999 C321 all TAG sites changes to UAG George...sites changes to UAG, RF1 function removed Farren Isaacs 87359 C321.∆A.opt all TAG sites changes to UAG...enzyme loads a specific amino acid to its tRNA. The charged tRNA is then recruited to the ribosome, where its...the tRNA is incorporated to the growing peptide chain. To expand the genetic code and incorporate a ncAA...

  10. Fluorescent Protein Guide: Subcellular Localization

    Type
    Collection
    ...Chromatin H2B mClover3 Michael Lin 74258 pKanCMV-mRuby3-10aa-H2B Chromatin H2B mRuby3 Michael Lin 224436 pCS2FA-H2B-mScarlet3...mEmerald* Michael Davidson 54491 mCherry-Lifeact-7 Actin filaments LifeAct mCherry* Michael Davidson 54610... EGFP* Michael Davidson 55148 mCherry-Tubulin-C-18 Microtubules alpha-Tubulin mCherry* Michael Davidson...mClover3 Michael Lin 74255 pKanCMV-mRuby3-18aa-actin Actin filaments beta-Actin mRuby3 Michael Lin 189767...mClover3 Michael Lin 74256 pKanCMV-mRuby3-18aa-Tubulin Microtubules alpha-Tubulin mRuby3 Michael Lin 189770...mClover3 Michael Lin 74254 pKanCMV-CX4.3-7aa-mRuby3 Gap junctions Connexin 43 mRuby3 Michael Lin 55023...mApple* Michael Davidson 55165 mCherry-ZO1-C-14 Tight junctions Zonula Occludens-1 mCherry* Michael Davidson...

  11. Brain Armamentarium

    Type
    Collection
    ...construct expressing mCherry-tagged channelrhodopsin-2 driven by chandelier cell-targeting enhancer Gordon ...213830-AAV1 pAAV_BiCHATe27_ChR2_mCherry AAV construct expressing mCherry-tagged channelrhodopsin-2 driven...213830-PHPeB pAAV_BiCHATe27_ChR2_mCherry AAV construct expressing mCherry-tagged channelrhodopsin-2 driven..._eGFP AAV construct expressing eGFP driven by chandelier cell-targeting enhancer. Alias: pAAV_WDC0004_...mCherry AAV construct expressing mCherry-tagged channelrhodopsin-2 driven by SST interneuron-targeting enhancer...

  12. CRISPR Guide

    Type
    Collection
    .... You can therefore change the genomic target of the Cas enzyme by simply changing the target sequence...surface-exposed, positively-charged grooves on Cas9. gRNA binding induces a conformational change in Cas9, shifting...Fanton, A., Tycko, J., Hinks, M., Chandrasekaran, S. S., Perry, N. T., Schaepe, J., Du, P. P., Lotfy, P., Bassik... gRNA. Figure 1: Overview of the basic CRISPR mechanism. CRISPR was originally employed to knock out target... In this section, we will introduce the basic mechanisms of CRISPR and some of the ways the CRISPR components...efficiency, or flexibility of the Cas enzyme. Basic Mechanisms of CRISPR The requirements for a CRISPR knockout...Using CRISPR Cas9 undergoes a second conformational change upon target binding. In this formation, its nuclease...

  13. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ..., a conformational change in the sensing region of the construct leads to a change in signal of a circularly... of membrane charges A FRET sensor enables quantitative measurements of membrane charges in live cells...affinities or negative-control constructs used to characterize the sensor's behavior in the target system. ...sensor behavior may differ from the original characterization (e.g. in vitro vs. in vivo, cytoplasmic vs...GECI) that reports with lifetime and intensity changes A turquoise fluorescence lifetime-based biosensor...calcium sensors (fRCaMP1/2, fRGECO1/2) The kinetic mechanisms of fast-decay red-fluorescent genetically encoded...near-infrared indicator iGECInano Design and Initial Characterization of a Small Near-Infrared Fluorescent Calcium...

  14. Antibody Guide

    Type
    Collection
    ...made up of four protein chains: two large heavy chains and two small light chains. These four domains come... producing antibodies, can change the constant region, and therefore change the isotype of antibodies ...: scFv - Single-chain fragment variables (scFvs) contain only the variable heavy chain and variable light... it is determined by a region called the “heavy chain constant region” or Fc. Different isotypes play ...light chain regions of an antibody. They are small enough to be produced in bacteria (~25 kD), but tend... derived from a camelid antibody called a heavy chain antibody (HCAb). Their small size allows them to... conjugation reaction. Many antibodies can be purchased already conjugated, which can save significant...

  15. Brain Initiative Collection

    Type
    Collection
    ...promoter. Used for photoswitchable control of inhibitory GPCR signaling cascades. Michael Bruchas 139505-AAV8...promoter. Used for photoswitchable control of inhibitory GPCR signaling cascades. Michael Bruchas 139505-AAV9...promoter. Used for photoswitchable control of inhibitory GPCR signaling cascades. Michael Bruchas 140135-AAV1...AAV9 pAAV-hSyn-DIO-ChrimsonR-mRuby2-ST Cation channelrhodopsin ChrimsonR fused to mRuby2 fluorophore and ...AAV9 pAAV-hSynapsin- soCoChR-GFP A somatic channelrhodopsin, which enables single cell, single millisecond... pAAV-hSynapsin-FLEX-soCoChR-GFP A somatic channelrhodopsin, which enables single cell, single millisecond...pAAV-CAG-DIO-ChroME-ST-P2A-H2B-mRuby3 Cation channelrhodopsin ChroME targeted to the neuronal soma and proximal...

  16. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ...-strand break. CRISPR systems, found in 95% of archaeal and 48% of bacterial genomes, are highly diverse...defines 5 types and 16 subtypes based on shared characteristics and evolutionary similarity. These are grouped... aeruginosa . Anti-CRISPR genes employ varied mechanisms to block CRISPR. Some prevent the CRISPR-Cas ...genome. Multiple Cas9 orthologs have also been characterized. Staphylococcus aureus (SaCas9) is smaller than... as guanosine, so the overall effect is an A->G change. Since this method targets RNA rather than DNA,...Xenopus References Barrangou R, Fremaux C, Deveau H, Richards M, Boyaval P, Moineau S, Romero DA, Horvath P....Wiedenheft B, Maxwell KL, Davidson AR. 2015. Multiple mechanisms for CRISPR-Cas inhibition by anti-CRISPR proteins...

  17. Bacterial Expression Systems

    Type
    Collection
    ...tdTomato (acceptor) FRET Robert Campbell , Michael Davidson Michael Davidson , Nathan Shaner , Roger Tsien...including photoactivatable, photoconvertible, and photoswitchable proteins, organized by color and expression...tdTomato (acceptor) FRET/Dual FRET Robert Campbell Michael Davidson , Nathan Shaner , Roger Tsien 54553 54723...mCitrine (acceptor) FRET/Dual FRET Robert Campbell , Michael Davidson 54571 54856 mT-Sapphire-pBAD tdTomato-...mRuby2-pBAD Clover (donor) mRuby2 (acceptor) FRET Michael Davidson 18856 pGWF1 ECFP (donor) Venus (acceptor...specific DNA sequences. ID Plasmid Reports Reporter Mechanism Expression Species PI 14474 pRU1161 Promoter activity...tdTomato) Mycobacterium sp. Tanya Parish 24658 pCHARGE3 Promoter activity Fluorescence (turbo-365) Mycobacterium...

  18. Neurodegeneration Research Collection

    Type
    Collection
    ...Neurodegenerative diseases are a group of disorders characterized by the progressive degeneration and ultimate... within 3–5 years from symptom onset. Disease mechanisms are still poorly understood, but several genes... of nerve cells in the brain. Symptoms include changes in behavior and emotion, uncontrolled movements...expression constructs: pLVX-UbC-rtTA-Ngn2:2A:EGFP (Schafer et al. Nat Neurosci. 2019) pLVX-UbC-rtTA-Ngn2:2A...window) Rodent Models (Link opens in a new window) Michael J Fox Foundation (Link opens in a new window) A...therapies for those living with Parkinson's today. The Michael J. Fox Foundation has made a number of tools (Link...provided by the foundation can be found on our Michael J. Fox Foundation Plasmid Resource page . CHDI ...

  19. Immunology Research Plasmids and Resources

    Type
    Collection
    ... 1 guanine nucleotide exchange factor VAV VAV2 vav 2 guanine nucleotide exchange factor - VAV3 vav 3 guanine... guanine nucleotide exchange factor FLJ40431 ZAP70 zeta-chain (TCR) associated protein kinase 70kDa FLJ17670...lab of Ian Frew . MXS Chaining Kit : From the lab of Pierre Neveu , the MXS-chaining system allows efficient...applications in mammalian systems. The MXS-chaining kit includes chaining vectors with multiple cloning sites...

  20. Optogenetics Plasmid Collection

    Type
    Collection
    ...opsins, which are membrane channels and pores derived from algae and archaebacteria, that can be opened and...widely used Channelrhodopsin-2 (ChR2) from Chlamydomonas reinhardtii . ChR2 is a membrane channel that opens...cations to flow into the cell. Variants of channelrhodopsin have been engineered or identified to increase...microbial opsins, such as the Halorhodopsins, Archaerhodopsins and Leptosphaeria rhodopsins, are pumps that...
Showing: 21 - 40 of 952 results