We narrowed to 997 results for: lars

...protocols from Addgene that can be used for basic molecular biology, plasmid cloning, and titering and testing...with any questions. Intro to the Lab Bench Basic Molecular Biology Plasmid Cloning Virus Antibodies Intro...weigh laboratory materials on a balance Basic Molecular Biology These protocols are the building blocks...

...Restriction enzyme digestion is commonly used in molecular cloning techniques, such as PCR or restriction...digest typically involves ∼500 ng of DNA, while molecular cloning often requires 1 µg of DNA. The total ... from 10-50 µL depending on application and is largely determined by the volume of DNA to be cut. A typical...will need to use a phosphatase to prevent re-circularization of the vector if you are cloning in an insert...sites . Sometimes enzymes cut sequences which are similar, but not identical, to their recognition sites....website about star activity . If you are digesting a large number of plasmids with the same enzyme(s) (for ...

...Pan-Gamma-protocadherin-Constant Mouse IgG2a 177504 Anti-LAR/PTPRF [N165/38R] LAR/PTPRF Human Mouse IgG2a 177505 Anti-PSD... Mouse IgG2b 225411 LAR/PTPRF (Homo sapiens) recombinant monoclonal antibody. LAR/PTPRF Human Mouse IgG2b... Anti-Pan-Neurofascin (extracellular) [A12/18R] Pan-Neurofascin (extracellular) Rat Mouse IgG2a 177439...Anti-Kv1.4 K+ channel (extracellular) [L71/5R] Kv1.4 K+ channel (extracellular) Rat Mouse IgG2a 177489...Mouse IgG2a 177564 Anti-ADAM22 (extracellular) [N57/2R] ADAM22 (extracellular) Mouse Mouse IgG2a 177565 Anti-GluN2B...Anti-Kv1.4 K+ channel (extracellular) [L71/22] Kv1.4 K+ channel (extracellular) Rat Mouse IgG2a 190292...-Neurofascin (extracellular) scFv [A12/18] A12/18 scFv 2t Pan-Neurofascin (extracellular) Rat Mouse 190486...

...Bygren, Lars O., et al. "Change in paternal grandmothers early food supply influenced cardiovascular mortality...PMC3929550. 3. Kaati, Gunnar, Lars O. Bygren, and Soren Edvinsson. "Cardiovascular and diabetes mortality determined...change in genotype. Epigenetic modifications play a large role in turning genes ‘off’ and ‘on’, signaling ...signaling which genes should be ‘read’ by the cellular machinery. This is how most cells in the body contain the...complex of DNA and histone proteins that compacts cellular DNA into chromosomes and reduces the risk of dangerous...the more compacted it becomes. This prevents the cellular machinery from physically interacting with the...Epigenetic changes have been discovered to play a large role in a variety of diseases, ranging from cancer...

...ATP (extracellular) ecAT3.10 extracellular ATP sensor (cell surface targeted) Imaging extracellular ATP...measurement of a biomolecule while largely preserving spatial and temporal cellular processes. Note that many ...green, red, or blue/green) Imaging intraorganellar Ca 2+ at subcellular resolution using CEPIA. Nat Commun...Yasushi Okada ATP (extracellular) GRAB_ATP1.0/ATP1.0mut biosensor for extracellular ATP (Constitutive ...encoded fluorescent sensor for measuring intracellular or extracellular glucose in vivo in mouse brain. Tim...Aug 20;524(7565):309-14. Richard Youle Osmolarity SED1 osmolarity biosensor, for expression in E. coli ...Eckhard Boles pH Imaging intracellular pH in live cells (pHRed) Imaging Intracellular pH in Live Cells with...

...both Cas12a and Cas3. Similarly, some Acr proteins are specific to a particular species of CRISPR enzymes... Browse Plasmids: Prime Edit Large Scale Edits CRISPR can generate large-scale genetic changes, including...Cas13a-based molecular detection platform. Based on this non-specific cleavage, a Cas13a-based molecular detection...CRISPR Guide Overview CRISPR Basics Small Edits Large Edits Genome-Wide Screening Cas9 Fusion Tools Cas9...techniques are referred to as CRISPR ( C lustered R egularly I nterspaced S hort P alindromic R epeat) technologies...and adaptability, CRISPR rapidly became the most popular genome engineering approach. Engineered CRISPR ...knockout or gene edit; or using Cas9 to generate large genomic deletions or inversions with two gRNA sites...

...PRKCG MTH Spinocerebellar ataxia 30 Frederic Mushinski 8418 pLTR PKC gamma PRKCG Spinocerebellar ataxia ...GFP CMV Spinocerebellar ataxia 27 Tobias Meyer 21204 GFP-N2-PKCgamma PRKCG GFP CMV Spinocerebellar ataxia...GFP CMV Spinocerebellar ataxia 25 Tobias Meyer 21215 GFP-C2gamma PRKCG GFP CMV Spinocerebellar ataxia ...CMV Spinocerebellar ataxia 23 Bernard Weinstein 21237 PKC gamma dNPS PRKCG HA CMV Spinocerebellar ataxia... CMV Spinocerebellar ataxia 21 Bernard Weinstein 21239 PKC gamma DN PRKCG HA CMV Spinocerebellar ataxia...CMV Spinocerebellar ataxia 15 Ryohei Yasuda 112270 CMV-PKCg-mEGFP-N1 PRKCG GFP CMV Spinocerebellar ataxia...GFP CMV Spinocerebellar Ataxia Richard Youle 176464 pVPS13D-I-EGFP VPS13D GFP CMV Spinocerebellar Ataxia...

...Johannes Stuttmann 1000000233 Joint Modular Cloning (JMC) Toolkit Modular cloning chassis and set of functional...Christopher Grefen 1000000222 MoBiFC Kit Modular bimolecular fluorescence complementation (MoBiFC) toolkit...plasmids and plasmid kits spanning a wide variety of molecular biology technologies: from genome engineering ...coding sequences, basic CRISPR parts, and parts for modular geminiviral replicon assembly. Golden Gate (MoClo...MoClo) Daniel Voytas 1000000156 MoChlo: Modular Cloning Chloroplast Toolbox Library of standardized chloroplast-specific...GreenGate) Alexis Maizel 1000000250 MultiGreen Kit Modular assembly of multi-gene expression vectors. Golden...following table contains a curated list of our most popular plasmids for gene editing in plants. Please refer...

...well-defined cellular sub-populations has generally been limited to single-component cellular definitions...addition to EYFP and ChR2-EYFP, a large number of additional, validated molecular payloads in the INTRSECT configuration..., Flp, and VCre to enable viral expression of molecular payloads in target populations defined by multiple...Plasmids References The development of powerful molecular reagents for biology have propelled our understanding...enabling combinatorial targeting) is a synthetic molecular targeting strategy that allows adeno-associated...multiple recombinase-expression strategies to define cellular sub-populations of interest, including dual-transgenic...

...resistance to photobleaching have made luciferase a popular tool for scientists. Regulatory elements such as... measured by the luminescence output. The most popular types of luciferase found in Addgene's collection...but are less-than-ideal protein tags due to their large size (61 kDa and 36 kDa, respectively). And they...be lysed or the substrate must be delivered intracellularly to measure luminescence. Gaussia luciferase...blog post . Empty Backbones Browse or search for popular empty backbone plasmids into which you can clone...reference level of expression. Browse or search for popular plasmids expressing luciferase. We also offer ready-to-use...new window) : The NanoLuc® enzyme is split into Large (LgBiT; 18 kDa) and Small (SmBiT; 11 amino acids...

... Genetic Edit References CRISPR ( C lustered R egularly I nterspaced S hort P alindromic R epeats) is ...edits, including point mutations, base changes, and large insertions or deletions. For more information on...efficiencies. The template sequences can also have a large impact on editing efficiency. So while testing multiple...experiments, testing multiple pegRNA designs is particularly important for prime editing. CRISPR Intereference... . Lentiviral vectors Lentiviral vectors are a popular option for producing stable cell lines, since the...of AAV vectors is that the packaging limit (how large of a DNA sequence you can actually package into ...lipids, electroporation, or an endosomal approach. Similar to the mRNA method described above, this method...

...87427 FBL-mEGFP AICSDP-13 mEGFP Fibrillarin Nucleolus (dense fibrillar component) 87428 MYH10-mEGFP AICSDP...Nucleophosmin Nucleolus (granular component) 133963 UBTF-HaloTag AICSDP-80 HaloTag Nucleolar transcription factor... generate fluorescently tagged proteins of key cellular structures in human cell lines. Depositor...CRISPR Protein Tagging Fluorescent Protein Guide Subcellular Localization Plasmids Protocols The Allen Institute... generate fluorescently tagged proteins of key cellular structures in human cell lines of their choice... to create cell lines with endogenously-tagged cellular structures. Addgene ID Plasmid Allen Institute...mEGFP AICSDP-50 mEGFP Nucleophosmin Nucleolus (granular component) 109120 GJA1-mEGFP AICSDP-43 mEGFP Connexin...

...Plasmids Collection Empty Backbones Collection Molecular Biology Guide CRISPR Guide Bacteria are commonly...contamination and facilitates extraction. Browse our most popular plasmids for protein purification. Commonly used... purification tags compatible with Golden Gate modular cloning (MoClo) for use in E. coli . Addgene Blog...Förster resonance energy transfer (FRET) and bimolecular fluorescence complementation (BiFC) are techniques...collection of Golden Gate compatible vectors for bimolecular fluorescence complementation experiments in plants... plants. The Zinc Finger Consortium Modular Assembly Kits (Addgene #1000000005, 1000000010) contain plasmids...nucleases. Addgene Blog Introduction to FRET Bimolecular Fluorescence Complementation Makes Visualizing...

...Alzheimer's disease, through large genome-wide association studies (GWAS) or other large scale studies. Researchers...these millions of patients results in billions of dollars spent each year. Unfortunately, the causes of most...with the disease and implicate a wide range of cellular processes. The vast majority of cases (90–95%)... Use the 3xFlag-eGFP-Flag-SETX construct for subcellular localization studies with GFP, or IP with Flag...central nervous system, peripheral nervous system, vesicular brain cells and more. Measure synaptic transmission...of fluorescent proteins, including Markers of Subcellular Localization , Optogenetics Plasmids , and Chemogenetics...supports the development of a diverse portfolio of biomolecular tools and emphasizes their rapid and broad dissemination...

...LTR Description PI 1780 pBABE-neo largeTcDNA MoMLV Expression of Large T antigen for creation of immortalized...about retroviral plasmids at Addgene, including popular packaging, envelope, and transfer plasmids. Viral... packaging gene env This collection highlights popular gamma-retroviral transfer, packaging, and envelope...and viral safety. Gamma-Retroviral Vector Guide Popular Gamma-Retroviral Transfer Plasmids Want a retroviral... mammalian cells. David Sabatini Return to top Popular Gamma-Retroviral Packaging Plasmids ID Plasmid ...MoMLV gag and pol . Patrick Salmon Return to top Popular Gamma-Retroviral Envelope Plasmids ID Plasmid Description...

...Promega scientists and designed to streamline molecular and cellular biology experimental workflows. The collection...NanoLuc fusions are useful for both intracellular and extracellular applications where you want minimal...structural complementation reporter system composed of a Large BiT (LgBiT; 18 kDa) subunit and a small complementary...energy transfer technique designed to measure molecular proximity in living cells. NanoBRET PPI assays...TE) assays (Link opens in a new window) have a similar premise, but examine the proximity of a chemical...

...with sequence similarity 3, member C GS3786, ILEI FAM3D family with sequence similarity 3, member D EF7...fms-related tyrosine kinase 1 (vascular endothelial growth factor/vascular permeability factor receptor)...NR1I1 VEGFA vascular endothelial growth factor A MGC70609, MVCD1, VEGF, VEGF-A, VPF VEGFB vascular endothelial...N-RAS, RASH1 ICAM1 intercellular adhesion molecule 1 BB2, CD54, P3.58 ICAM2 intercellular adhesion molecule...The innate immune system is not specific to any particular pathogen. It includes physical barriers such ...immune response is antigen-specific and includes cellular and humoral immunity. Once an adaptive immune ...HSPA10, LAP1, MGC131511, MGC29929, NIP71 ICAM1 intercellular adhesion molecule 1 BB2, CD54, P3.58 IFI30 interferon...

... for clarity and does not indicate a specific relationship. p53 Pathway Color is used for clarity and ...transcription factor and tumor suppressor activated by cellular stress. Cancer...transcription factor and tumor suppressor activated by cellular stresses like DNA damage, oxidative stress, and...damaged cells. The p53 name arose from its observed molecular weight (53 kDa) when run on an SDS-PAGE gel. Cancer...on functional p53 through oligomerization. In particular, the loss of p53’s pro-apoptotic effects is especially...only one functional copy of the p53 gene causes a large reduction in tumor suppression activity, leading...

...Polymerase I, Large (Klenow) Fragment 165522 pOpen-K12polLF (exo-) DNA Polymerase I, Large (Klenow) Fragment...plasmids at Addgene for generating enzymes for molecular biology lab work. Depositor...for enzymes that are considered "workhorses" of molecular biology, including DNA polymerases, RNA polymerases... lab costs and overcome supply chain issues, particularly in areas of the world where enzymes are harder...Polymerase 165553 pOpen-BstpolLF Bst DNA Polymerase, Large Fragment 165562 pOpen-Pwopol Pwo DNA Polymerase ...Polymerase 165563 pOpen-BsupolLF Bsu DNA Polymerase I, Large Fragment 165570 pOpen-P45 P45 165572 pOpen-GBDpol ...

...acceptor molecule (also referred to as intermolecular or bimolecular FRET) or (2) conformational changes ...the same protein (also referred to as intramolecular or unimolecular FRET). Learn more about FRET on our...small molecules, and more! Popular FRET Pairs This table lists some popular fluorescent proteins used ...Vogel, S. S., & Medintz, I. L. (2019). FRET as a biomolecular research tool — understanding its potential ...