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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 779 results for: mos

Showing: 21 - 40 of 779 results

  1. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...presence of specific molecules or other factors. Most often, a conformational change in the sensing region... astrocyte calcium signaling in the hippocampal mossy fiber pathway. Neuron. 2014 Apr 16;82(2):413-29....from ATP-Mg Hydrolysis Contributes to Mitotic Chromosome Condensation. Curr Biol. 2018 Feb 5;28(3):444... the intracellular environment of S. cerevisiae MOSAIC Kit includes 35 plasmids expressing a variety of...

  2. CRISPR Plasmids - Parasites

    Type
    Collection
    ...Parasites such as Plasmodium (malaria), Toxoplasma (toxoplasmosis), Trypanosoma (African sleeping sickness), Cryptosporidium...homology-directed repair (HDR) instead of NHEJ. In most experimental systems, HDR occurs at a much lower...

  4. DNA Service - Cloning Grade DNA

    Type
    Collection
    ...by ordering cloning grade DNA for over 200 of our most popular plasmids!...but helps to inform us of which plasmids would be most helpful to requesting scientists. Return to top ...

  5. Chemogenetics Plasmids

    Type
    Collection
    ...Selective Actuator Modules (PSAMs). DREADDs are the most widely used chemogenetic receptors and are G-coupled...recently developed class of tools are Luminopsins (LMOs), or luminescent opsins: fusion proteins of a light-emitting...

  6. Synthetic Biology - Assembly Standards Guide

    Type
    Collection
    ... provides information for the assembly standards most commonly used by part registries, including the ... Cloning Techniques Guide for an overview of the most common methods. BioBrick BioBrick-2 BglBrick Silver...

  7. Adenovirus Plasmids

    Type
    Collection
    ...range of dividing and non-dividing cell types with almost 100% efficiency. Unlike lentiviruses or other retroviruses...Adenoviruses can be produced by several methods, the most common of which involves homologous recombination...

  8. p53 Pathway

    Type
    Collection
    ...Cancer Impact p53 is the most frequently mutated gene (>50%) in human cancer; most of these mutations are...

  9. TALEN Guide

    Type
    Collection
    ...region of DNA in the genome of your favorite species almost as easily as correcting a typo in the latest draft...make arrays from 12-31 repeats. Perhaps one of the most appealing features of the TAL effector arrays is...

  10. Ras Pathway

    Type
    Collection
    ...small GTPase and constitutively active Ras is the most common oncogene in human cancer....leading to cancer. Constitutively active Ras is the most common oncogene in human cancer, found in around...

  11. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ...selection of Addgene's empty vector backbones. For the most part, we will assume that you want to express a ... - An improved agroinfection-compatible Tobacco mosaic virus (TMV)-based transient expression vector GFP...

  12. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ... The beauty of CRISPR is that it’s adaptable to almost any model system or biological topic, and you don...Pederson T. 2015. Multicolor CRISPR labeling of chromosomal loci in human cells. Proc Natl Acad Sci U S A...

  13. Bacterial Expression Systems

    Type
    Collection
    ...interesting biological questions. Escherichia coli is the most widely used bacterial organism due to its ease of...contamination and facilitates extraction. Browse our most popular plasmids for protein purification. Commonly...

  14. Tetracycline Inducible Expression

    Type
    Collection
    ...targets. The tetracycline (tet) system is one of the most popular choices for rapid, efficient, and reversible...sequences with elements like the H1 RNA promoter. And, most importantly, the TetR protein has been modified ...

  15. Fluorescent Protein Guide: Subcellular Localization

    Type
    Collection
    ...determining subcellular localization. One of the most common is to use fluorescence microscopy to compare...junctions, gap junctions, tight junctions, and desmosomes. Browse or search the table below to find markers...

  16. Viral Vectors 101: Pseudotyping

    Type
    Blog Post
    ...Bieniasz PD, Jäger U, Porter CD, Friedman T, McClure MO, Weiss RA (1997) Sensitization of rhabdo-, lenti-...so commonly used is that it is good at infecting most cells, but not all. For example, LDLR expression...envelope proteins for pseudotyping While VSV-G is the most commonly used envelope protein for pseudotyping,...

  17. Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone

    Type
    Blog Post
    ...Susanna Harris, Heather Hendrickson, Jonathan Jacobs, Mo Kaze, Christina Kellogg, Heather Martinez, Pat Schloss...don’t have the networks or experience to get the most of meeting.  I experienced this at the ASM General...and helping societies help their members get the most out of their meetings. Amanda Gunn ran randomized...

  18. CRISPR Library Amplification

    Type
    Protocol
    ...than four Maxipreps worth. Count colonies on the most dilute Petri dish. Total colony yield = count x ...in library. Example: At least 10 colonies on the most diluted plate for 1000x coverage of a library of... are present. If colonies are not present on the most dilute plate, count the second plate (ie. the 1:...tend to report lower values than Nanodrop, although most protocols have been designed with Nanodrop as the...

  19. Protocol - How to Ligate Plasmid DNA

    Type
    Protocol
    ...been generated by restriction enzyme digestion . Most restriction enzymes digest DNA asymmetrically across...size of each and their concentration. However, for most standard cloning (where the insert is smaller than... + Background due to vector re-circularization - most useful for phosphatase treated vector Insert or ...

  20. Using a Light Microscope Protocol

    Type
    Protocol
    ...so, for this protocol, we'll focus on one of the most common types that you're likely to encounter: the...properties of light to detect small objects. Two of the most important properties of a microscope are magnification... will see several pieces of information, but the most important (for now) is the magnification power, ...
Showing: 21 - 40 of 779 results