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Showing: 21 - 40 of 954 results

  1. Writing Scientific Manuscripts: Literature Searching, Reading, & Organizing

    Type
    Blog Post
    ...send you daily updates with relevant literature. Organizing the literature As a scientist, you will begin...will ever read. So, you need to come up with an organization system that works for you. I have a folder where...’m still in the dark ages with respect to my organization strategy. There are a number of software options...another option that may help solve your literature organization issues – F1000 just announced the launch of ....  Give your favorite searching, reading and organization tips in the comments below. Additionally, read...

  2. Three Tips to Organize Your Lab Notebooks in the New Year

    Type
    Blog Post
    ...next R01 is due. But what about organizing yourself and your lab? Organizing your lab notebooks, protocols...you think! Here are three tips to help you get organized and boost your productivity in 2014. Tip 1: Use...and no closer to starting your experiments. Get organized now! Start by gathering that pile of lab notebooks...your tasks on the go Never miss a deadline again. Organize you experiments, keep track of upcoming grant ... clippings, photographs, and file attachments. Organize your notes into notebooks, create checklists that...spreadsheets with lab members Now that you’ve organized your protocols, wouldn’t it be great if you could...reagent storage locations, such as the list used to organize your cryo freezer. No more issues deciphering ...

  3. What's Your Organism? Expanding Genomic Tools via the NSF EDGE Program

    Type
    Blog Post
    ... model organism blog posts: Five Popular Model Organisms, Part 1 Five Popular Model Organisms, Part 2...diverse organisms. Methods for genetic manipulation still need to be developed for each organism, but researchers...and share all of these new organisms continues to be challenging. Organism specific viral vector systems...programs via Addgene. New organisms for genotype to phenotype studies New organismal systems described at ...and shared for many different organisms. Do you have a favorite organism you study in the lab? Let us ... work but never get to actually manipulate the organism they study (unless if you count normal human interaction...spiny purple sea urchin. It’s not a standard model organism, but it was easy to harvest eggs and sperm, mix...

  4. Open Resources and Plasmid Tools For Studying C. elegans

    Type
    Blog Post
    ...PMCID: PMC3531189. Mangone, Marco, et al. "UTRome. org: a platform for 3′ UTR biology in C. elegans." Nucleic...source project that is creating the “first virtual organism in a computer.” This project has produced various..., we have the entire SapTrap toolkit from the Jorgensen lab. SapTrap is a reagent toolkit and plasmid ...repair template of your choosing (Schwartz and Jorgensen, 2016). Using SapTrap, this CRISPR plasmid can...published in a microPublication (Schwartz and Jorgensen, 2018).  These are just some examples of worm ...PMC2238901. Schwartz, Matthew L., and Erik M. Jorgensen. "SapTrap, a toolkit for high-throughput CRISPR...Central PMCID: PMC4905529.  Schwartz, M. L., & Jorgensen, E. M. "SapTrap vectors for introducing point ...

  5. Chemogenetics Guide

    Type
    Guide
    ...that both KORD and hM3Dq can be used in the same organism to allow bidirectional control of neuronal activity...Biology. Physiol Rev, 98 (1), 391–418. https://doi.org/10.1152/physrev.00009.2017 PMID: 29351511 Armbruster...Natl Acad Sci USA, 104 (12), 5163–5168. https://doi.org/10.1073/pnas.0700293104 PMID: 17360345 Berglund...Natl Acad Sci USA, 113 (3), E358–E367. https://doi.org/10.1073/pnas.1510899113 PMID: 26733686 Björefeldt...bioluminescence. Neurophotonics 11 (2), 021005. https://doi.org/10.1117/1.NPh.11.2.021005 PMID: 38450294 Coward...Proc Natl Acad Sci USA, 95 (1), 352–357. https://doi.org/10.1073/pnas.95.1.352 PMID: 9419379 Davies, M. ... Psychopharmacology, 178 (4), 451–460. https://doi.org/10.1007/s00213-004-2017-1 PMID: 15765260 Farrell...

  6. Adeno-associated virus (AAV) Guide

    Type
    Guide
    ...AAV vectors . Microorganisms, 12 (2), 384. https://doi.org/10.3390/microorganisms12020384 (Link opens in...genes. rAAV episomes can develop chromatin-like organization and persist in non-dividing cells for a period...different serotype(s) for transduction of a given organ. Tissue Serotype Central nervous system AAV1, AAV2...administration, distributing widely throughout the organism to target large or diffused biological structures... of systemic rAAV vectors that can target whole organs or specific cell types of the CNS (e.g. AAV-PHP.eB...Packaged-on-Request AAV Preps In-Stock AAV Preps Resources on addgene.org AAV Plasmids Available at Addgene Viral Vectors... . Molecular Therapy, 10 (2), 302–317. https://doi.org/10.1016/j.ymthe.2004.05.024 (Link opens in a new...

  7. Lentiviral Vector Guide

    Type
    Guide
    ...safety. Resources and References Resources on addgene.org Lentiviral Plasmids Collection In-Stock Lentiviral...Current Gene Therapy , 5 (4), 387–398. https://doi.org/10.2174/1566523054546224 PMID: 16101513 Dull, T...Journal of Virology , 72 (11), 8463–8471. https://doi.org/10.1128/jvi.72.11.8463-8471.1998 PMID: 9765382 ...lentiviral vectors. Viruses , 3 (2), 132–159. https://doi.org/10.3390/v3020132 PMID: 22049307 Ghaleh, H. E. G...Biomedicine & Pharmacotherapy , 128 , 110276. https://doi.org/10.1016/j.biopha.2020.110276 PMID: 32502836 Johnson...& Clinical Development , 21 , 451–465. https://doi.org/10.1016/j.omtm.2021.03.018 PMID: 33981779 Kim, ...& Clinical Development , 20 , 792–800. https://doi.org/10.1016/j.omtm.2020.10.012 PMID: 33768124 Marshall...

  8. Adenovirus Guide

    Type
    Guide
    ...safety. Resources and References Resources on addgene.org Adenoviral Plasmids Available at Addgene AdenoBuilder...Current Gene Therapy, 11 (4), 307–320. https://doi.org/10.2174/156652311796150372 (Link opens in a new...Transduction and Targeted Therapy, 6 (1), 53. https://doi.org/10.1038/s41392-021-00487-6 (Link opens in a new...vaccine . Scientific Reports, 10 (1), 3613. https://doi.org/10.1038/s41598-020-60238-5 (Link opens in a new...Immunotherapeutics, 13 (12), 3020–3032. https://doi.org/10.1080/21645515.2017.1383575 (Link opens in a ...Journal of Virology, 85 (9), 4222–4233. https://doi.org/10.1128/JVI.02407-10 (Link opens in a new window...Review of Vaccines, 14 (10), 1347–1357. https://doi.org/10.1586/14760584.2015.1077122 (Link opens in a ...

  9. Plan Your Experiment

    Type
    Guide
    ...researchers can adapt these experiments for different organisms. This page provides a general framework to get...principles also apply to using CRISPR in other organisms. As with most experiments, you first need to have...in Molecular Biology , 1239 , 197–217. https://doi.org/10.1007/978-1-4939-1862-1_10 PMID: 25408407 Dixit...Screens. Cell , 167 (7), 1853–1866.e17. https://doi.org/10.1016/j.cell.2016.11.038 PMID: 27984732 Doman...Nature Protocols , 17 (11), 2431–2468. https://doi.org/10.1038/s41596-022-00724-4 PMID: 35941224 Gilbert...and activation. Cell , 159 (3), 647–661. https://doi.org/10.1016/j.cell.2014.09.029 PMID: 25307932 Glass...Trends in Biotechnology , 36 (2), 173–185. https://doi.org/10.1016/j.tibtech.2017.11.006 PMID: 25307932 Hanna...

  10. Gamma-Retroviral Vector Guide

    Type
    Guide
    ...safety. Resources and References Resources on addgene.org Gamma-retroviral Plasmids Collection Viral Vectors...Journal of Virology , 88 (8), 4504–4513. https://doi.org/10.1128/jvi.00011-14 PMID: 24501411 Gilroy, K. ...genomics. PLoS ONE , 11 (4), e0154070. https://doi.org/10.1371/journal.pone.0154070 PMID: 27097319 LaFave...Nucleic Acids Research , 42 (7), 4257–4269. https://doi.org/10.1093/nar/gkt1399 PMID: 24464997 Maetzig, T.,...application. Viruses , 3 (6), 677–713. https://doi.org/10.3390/v3060677 PMID: 21994751 Mekkaoui, L., Tejerizo...& Clinical Development , 28 , 116–128. https://doi.org/10.1016/j.omtm.2022.12.006 PMID: 36620071 Mirow...GALV envelope. Viruses , 13 (8), 1471. https://doi.org/10.3390/v13081471 PMID: 34452336 Schnierle, B. ...

  11. Modular Cloning Guide

    Type
    Guide
    ...at 18 levels for rapid characterization in new organisms. Mobius Assembly Vector Toolkit Bacterial Expression...Toolbox Kits Bacterial Expression Thorsten Mascher , Georg Fritz Two sets of plasmids to generate constructs...reconstituting the ubiquitination cascades of different organisms in E. coli . MetClo DNA Assembly Kit Bacterial...Platinum TALENs for use in a wide variety of target organisms. TALEN Kit Genome Engineering, TALEN Stephen Ekker...fusion proteins for use in many different model organisms. Diatom uLoop Assembly Kit Other, Yeast Expression...tags, and signal peptides, for use in a range of organisms including diatoms, yeast, plants and bacteria....

  12. Tetracycline Inducible Expression

    Type
    Collection
    ...SP-dCas9-VPR transcriptional activator with Tet-On 3G George Church 87360 TLCV2 Lentiviral vector for tet-inducible...Expression . Curr Gene Ther, 16 (3), 156–167. https://doi.org/10.2174/1566523216666160524144041 (Link opens in...Natl Acad Sci U S A, 89 (12), 5547–5551. https://doi.org/10.1073/pnas.89.12.5547 (Link opens in a new window...cells . Science, 268 (5218), 1766–1769. https://doi.org/10.1126/science.7792603 (Link opens in a new window...range . Hum Gene Ther, 22 (2), 166–176. https://doi.org/10.1089/hum.2010.099 (Link opens in a new window...transregulators . BioTechniques, 37 (4), 546–550. https://doi.org/10.2144/04374BM04 (Link opens in a new window) ... expression . BMC Biotechnol, 10 , 81. https://doi.org/10.1186/1472-6750-10-81 (Link opens in a new window...

  13. Plan Your Experiment

    Type
    Collection
    ...researchers can adapt these experiments for different organisms. This page provides a general framework to get...principles also apply to using CRISPR in other organisms. As with most experiments, you first need to have...in Molecular Biology , 1239 , 197–217. https://doi.org/10.1007/978-1-4939-1862-1_10 PMID: 25408407 Dixit...Screens. Cell , 167 (7), 1853–1866.e17. https://doi.org/10.1016/j.cell.2016.11.038 PMID: 27984732 Doman...Nature Protocols , 17 (11), 2431–2468. https://doi.org/10.1038/s41596-022-00724-4 PMID: 35941224 Gilbert...and activation. Cell , 159 (3), 647–661. https://doi.org/10.1016/j.cell.2014.09.029 PMID: 25307932 Glass...Trends in Biotechnology , 36 (2), 173–185. https://doi.org/10.1016/j.tibtech.2017.11.006 PMID: 25307932 Hanna...

  14. Rinehart Lab Phosphoprotein Reagents

    Type
    Collection
    .... Nature Methods , 19 (11), 1371–1375. https://doi.org/10.1038/s41592-022-01638-5. PMID 36280721 (Link... . Molecular Systems Biology , 19 (8). https://doi.org/10.15252/msb.202110591. PMID 37477096 (Link opens...Nature Biotechnology , 36 (7), 638–644. https://doi.org/10.1038/nbt.4150. PMID 29889213 (Link opens in ...library . Biochemistry , 57 (31), 4717–4725. https://doi.org/10.1021/acs.biochem.8b00410. PMID 29920078 (Link...phosphorylation . Nature Communications , 6 (1). https://doi.org/10.1038/ncomms9130. PMID 26350500 (Link opens in...synthesis . Nature Communications , 6 (1). https://doi.org/10.1038/ncomms9168. PMID 26350765 (Link opens in...mitophagy . Molecular Cell , 60 (1), 7–20. https://doi.org/10.1016/j.molcel.2015.08.016. PMID 26365381 (Link...

  15. Zebrafish Plasmid Collection

    Type
    Collection
    ...been established as a powerful vertebrate model organism for the study of genetics and developmental biology...Multicolor fluorescent labeling in zebrafish Whole organism lineage tracing by combinatorial and cumulative... previously resistant cell types. CreLite - George Eisenhoffer Lab. An optogenetically controlled ...screening fluorescent protein fusion expression in organisms or cells in which an exogenous mRNA can be injected...assembly for the zebrafish genome. Zebrafish Model Organism Database (ZFIN) - A curated database of genetic... International Zebrafish Society (IZFS) - An organization that promotes and advocates for zebrafish research...aims to increase the use of zebrafish as a model organism and to foster advanced training of young scientists...

  16. Fluorescent Proteins: FRET

    Type
    Collection
    ... on protein binding or conformational changes. Organic dyes, fluorescent proteins, and other molecules...10 15 M -1 cm -1 nm 4 ). Values obtained from FPbase.org FRET calculator (Link opens in a new window) ...can be influenced by many factors, such as the organism or cell type, relative position and orientation...FRET pairs and biosensors can also incorporate organic fluorophores via HaloTag or SNAP-Tag . Read more...FRET Pairs . Sensors , 16 (9), 1488. doi: https://doi.org/10.3390/s16091488 (Link opens in a new window) ...Nature Methods , 16 (9), 815–829. doi: https://doi.org/10.1038/s41592-019-0530-8 (Link opens in a new ...Link opens in a new window) External Resources FPbase.org FRET calculator (Link opens in a new window) ...

  17. COVID-19 Resources

    Type
    Collection
    ...-2 . Nature medicine, 26 (4), 450–452. https://doi.org/10.1038/s41591-020-0820-9 (Link opens in a new ...virology, 82 (17), 8887–8890. https://doi.org/10.1128/JVI.00415-08 (Link opens in a new window...Journal of virology, 88 (2), 1293–1307. https://doi.org/10.1128/JVI.02202-13 (Link opens in a new window...Inhibitor . Cell, 181 (2), 271–280.e8. https://doi.org/10.1016/j.cell.2020.02.052 (Link opens in a new...journal of molecular sciences, 21 (6), 1947. https://doi.org/10.3390/ijms21061947 (Link opens in a new window...transduction and targeted therapy, 5 (1), 283. https://doi.org/10.1038/s41392-020-00426-x (Link opens in a new...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...

  18. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...Scaffold for inorganic phosphate biosensor Development of a Reagentless Biosensor for Inorganic Phosphate...biosensors can be introduced into cells, tissues, or organisms for long-term imaging and can be designed to specifically...specifically localize to cellular compartments or organelles. Addgene’s database includes many fluorescent...vs. in vivo, cytoplasmic vs. extracellular vs. organelle-localized). Browse, sort, or search the tables...and In Vivo Imaging of Ca Dynamics in High-Ca Organelles. Cell Chem Biol. 2016 Jun 1. pii: S2451-9456(...sensors (green, red or blue/green) Imaging intraorganellar Ca(2+) at subcellular resolution using CEPIA...Genetically Encoded FRET Sensor for Cytosolic and Intraorganelle Zn(2+) Imaging. ACS Chem Biol. 2015 Sep 18;...

  19. Fluorescent Protein Guide: Subcellular Localization

    Type
    Collection
    ... structures give the cell its shape, organize cellular organelles, and are necessary for movement and ...localization patterns for colocalization studies or organelle labeling. Plasmid... changes to particular cellular structures or organelles, such as remodeling the cytoskeleton or blocking... fusion proteins that are targeted to various organelles or subcellular structures. To compare with a ...Structures Eukaryotic cells contain a variety of organelles and subcellular structures, each with their own...table below to find markers of general cellular organelles and structures. ID Plasmid Structure Targeting...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...

  20. Worm Expression Resources

    Type
    Collection
    ... “the worm”) is widely used as a genetic model organism due to their rapid life cycle (3 days from egg...Lab website . SapTrap CRISPR/Cas Toolkit - Erik Jorgensen Lab. A collection of plasmids that streamlines... Transgenesis miniMos Transgenesis Kit - Erik Jorgensen Lab. This kit contains 29 plasmids used to generate... related miniMos plasmids can be found on the Jorgensen Lab page. More information on miniMos and MosSCI...methods to engineer the genome of the genetic model organism C. elegans. Return to top Do you have suggestions...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...
Showing: 21 - 40 of 954 results