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We narrowed to 947 results for: org

Showing: 21 - 40 of 947 results

  1. Writing Scientific Manuscripts: Literature Searching, Reading, & Organizing

    Type
    Blog Post
    ...send you daily updates with relevant literature. Organizing the literature As a scientist, you will begin...will ever read. So, you need to come up with an organization system that works for you. I have a folder where...’m still in the dark ages with respect to my organization strategy. There are a number of software options...another option that may help solve your literature organization issues – F1000 just announced the launch of ....  Give your favorite searching, reading and organization tips in the comments below. Additionally, read...

  2. Three Tips to Organize Your Lab Notebooks in the New Year

    Type
    Blog Post
    ...next R01 is due. But what about organizing yourself and your lab? Organizing your lab notebooks, protocols...you think! Here are three tips to help you get organized and boost your productivity in 2014. Tip 1: Use...and no closer to starting your experiments. Get organized now! Start by gathering that pile of lab notebooks...your tasks on the go Never miss a deadline again. Organize you experiments, keep track of upcoming grant ... clippings, photographs, and file attachments. Organize your notes into notebooks, create checklists that...spreadsheets with lab members Now that you’ve organized your protocols, wouldn’t it be great if you could...reagent storage locations, such as the list used to organize your cryo freezer. No more issues deciphering ...

  3. What's Your Organism? Expanding Genomic Tools via the NSF EDGE Program

    Type
    Blog Post
    ... model organism blog posts: Five Popular Model Organisms, Part 1 Five Popular Model Organisms, Part 2...diverse organisms. Methods for genetic manipulation still need to be developed for each organism, but researchers...and share all of these new organisms continues to be challenging. Organism specific viral vector systems...programs via Addgene. New organisms for genotype to phenotype studies New organismal systems described at ...and shared for many different organisms. Do you have a favorite organism you study in the lab? Let us ... work but never get to actually manipulate the organism they study (unless if you count normal human interaction...spiny purple sea urchin. It’s not a standard model organism, but it was easy to harvest eggs and sperm, mix...

  4. Open Resources and Plasmid Tools For Studying C. elegans

    Type
    Blog Post
    ...PMCID: PMC3531189. Mangone, Marco, et al. "UTRome. org: a platform for 3′ UTR biology in C. elegans." Nucleic...source project that is creating the “first virtual organism in a computer.” This project has produced various..., we have the entire SapTrap toolkit from the Jorgensen lab. SapTrap is a reagent toolkit and plasmid ...repair template of your choosing (Schwartz and Jorgensen, 2016). Using SapTrap, this CRISPR plasmid can...published in a microPublication (Schwartz and Jorgensen, 2018).  These are just some examples of worm ...PMC2238901. Schwartz, Matthew L., and Erik M. Jorgensen. "SapTrap, a toolkit for high-throughput CRISPR...Central PMCID: PMC4905529.  Schwartz, M. L., & Jorgensen, E. M. "SapTrap vectors for introducing point ...

  5. Tetracycline Inducible Expression

    Type
    Collection
    ...SP-dCas9-VPR transcriptional activator with Tet-On 3G George Church 87360 TLCV2 Lentiviral vector for tet-inducible...Expression . Curr Gene Ther, 16 (3), 156–167. https://doi.org/10.2174/1566523216666160524144041 (Link opens in...Natl Acad Sci U S A, 89 (12), 5547–5551. https://doi.org/10.1073/pnas.89.12.5547 (Link opens in a new window...cells . Science, 268 (5218), 1766–1769. https://doi.org/10.1126/science.7792603 (Link opens in a new window...range . Hum Gene Ther, 22 (2), 166–176. https://doi.org/10.1089/hum.2010.099 (Link opens in a new window...transregulators . BioTechniques, 37 (4), 546–550. https://doi.org/10.2144/04374BM04 (Link opens in a new window) ... expression . BMC Biotechnol, 10 , 81. https://doi.org/10.1186/1472-6750-10-81 (Link opens in a new window...

  6. Plan Your Experiment

    Type
    Collection
    ...researchers can adapt these experiments for different organisms. This page provides a general framework to get...principles also apply to using CRISPR in other organisms. As with most experiments, you first need to have...in Molecular Biology , 1239 , 197–217. https://doi.org/10.1007/978-1-4939-1862-1_10 PMID: 25408407 Dixit...Screens. Cell , 167 (7), 1853–1866.e17. https://doi.org/10.1016/j.cell.2016.11.038 PMID: 27984732 Doman...Nature Protocols , 17 (11), 2431–2468. https://doi.org/10.1038/s41596-022-00724-4 PMID: 35941224 Gilbert...and activation. Cell , 159 (3), 647–661. https://doi.org/10.1016/j.cell.2014.09.029 PMID: 25307932 Glass...Trends in Biotechnology , 36 (2), 173–185. https://doi.org/10.1016/j.tibtech.2017.11.006 PMID: 25307932 Hanna...

  7. Rinehart Lab Phosphoprotein Reagents

    Type
    Collection
    .... Nature Methods , 19 (11), 1371–1375. https://doi.org/10.1038/s41592-022-01638-5. PMID 36280721 (Link... . Molecular Systems Biology , 19 (8). https://doi.org/10.15252/msb.202110591. PMID 37477096 (Link opens...Nature Biotechnology , 36 (7), 638–644. https://doi.org/10.1038/nbt.4150. PMID 29889213 (Link opens in ...library . Biochemistry , 57 (31), 4717–4725. https://doi.org/10.1021/acs.biochem.8b00410. PMID 29920078 (Link...phosphorylation . Nature Communications , 6 (1). https://doi.org/10.1038/ncomms9130. PMID 26350500 (Link opens in...synthesis . Nature Communications , 6 (1). https://doi.org/10.1038/ncomms9168. PMID 26350765 (Link opens in...mitophagy . Molecular Cell , 60 (1), 7–20. https://doi.org/10.1016/j.molcel.2015.08.016. PMID 26365381 (Link...

  8. Zebrafish Plasmid Collection

    Type
    Collection
    ...been established as a powerful vertebrate model organism for the study of genetics and developmental biology...Multicolor fluorescent labeling in zebrafish Whole organism lineage tracing by combinatorial and cumulative... previously resistant cell types. CreLite - George Eisenhoffer Lab. An optogenetically controlled ...screening fluorescent protein fusion expression in organisms or cells in which an exogenous mRNA can be injected...assembly for the zebrafish genome. Zebrafish Model Organism Database (ZFIN) - A curated database of genetic... International Zebrafish Society (IZFS) - An organization that promotes and advocates for zebrafish research...aims to increase the use of zebrafish as a model organism and to foster advanced training of young scientists...

  9. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...Scaffold for inorganic phosphate biosensor Development of a Reagentless Biosensor for Inorganic Phosphate...biosensors can be introduced into cells, tissues, or organisms for long-term imaging and can be designed to specifically...specifically localize to cellular compartments or organelles. Addgene’s database includes many fluorescent...vs. in vivo, cytoplasmic vs. extracellular vs. organelle-localized). Browse or search the tables below ...and In Vivo Imaging of Ca Dynamics in High-Ca Organelles. Cell Chem Biol. 2016 Jun 1. pii: S2451-9456(...sensors (green, red or blue/green) Imaging intraorganellar Ca(2+) at subcellular resolution using CEPIA...Genetically Encoded FRET Sensor for Cytosolic and Intraorganelle Zn(2+) Imaging. ACS Chem Biol. 2015 Sep 18;...

  10. Worm Expression Resources

    Type
    Collection
    ... “the worm”) is widely used as a genetic model organism due to their rapid life cycle (3 days from egg...Lab website . SapTrap CRISPR/Cas Toolkit - Erik Jorgensen Lab. A collection of plasmids that streamlines... Transgenesis miniMos Transgenesis Kit - Erik Jorgensen Lab. This kit contains 29 plasmids used to generate... related miniMos plasmids can be found on the Jorgensen Lab page. More information on miniMos and MosSCI...methods to engineer the genome of the genetic model organism C. elegans. Return to top Do you have suggestions...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...

  11. Genetic Code Expansion

    Type
    Collection
    ...RF1 function removed George Church 48999 C321 all TAG sites changes to UAG George Church 49018 C321.ΔA.exp...pairs of synthetase and tRNA that work for one organism may not work for another. The orthogonal synthetase...removed, MutS restored so decreased mutation rate George Church 68306 C321.ΔA all TAG sites changes to UAG..., RF1 function removed, improved doubling time. George Church 98564 C321.Ub-UAG-sfGFP all TAG sites changes...function removed, with Ubiquitin-UAG-sfGFP reporter George Church 98565 C321.ΔClpS.Ub-UAG-sfGFP all TAG sites... inactivated, with Ubiquitin-UAG-sfGFP reporter George Church 174513 Syn61 No TCG, TCA, or TAG codons ...codons. Deletion of serT, serU, prfA, and recA genes George Church 192872 rEcoli XpS All 321 UAG codons changed...

  12. Fluorescent Protein Guide: Subcellular Localization

    Type
    Collection
    ... structures give the cell its shape, organize cellular organelles, and are necessary for movement and ... Plasmid Collections Fluorescent Proteins Organelle Markers Fluorescent Proteins: Subcellular Localization... fusion proteins that are targeted to various organelles or subcellular structures. You should fuse your...General Structures The cell contains a variety of organelles and subcellular structures each with their own...table below to find markers of general cellular organelles and structures. ID Plasmid Structure Targeting...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...

  13. CRISPR References and Information

    Type
    Collection
    ...sequence or list of sequences. Developed by the George Church Lab and maintained by the Frederick National...Supports a range of Cas enzymes (Cas9 and Cas12) and organisms. Developed by the Jin-Soo Kim Lab . Cas-Designer...target site common to a set of similar sequences. Organisms include human, mouse, rat, chicken, frog, zebrafish...upload. Currently supports a wide selection of organisms. From the Database Center for Life Science (DBCLS...off-target binding. Currently supports a wide range of organims. Developed by the Michael Boutros Lab . CasFinder...Currently supports: mouse and human. Developed by the George Church Lab . CRISPR Optimal Target Finder (Link...

  14. Botman-Teusink Yeast FP Collection

    Type
    Collection
    ...in budding yeast. Sci Rep, 9 (1):2234. https://doi.org/10.1038/s41598-019-38913-z (Link opens in a new...S. cerevisiae. PLoS One, 8 (7):e67902. https://doi.org/10.1371/journal.pone.0067902 (Link opens in a new...ammonium uptake. Nature, 446 (7132):195-8. https://doi.org/10.1038/nature05579 (Link opens in a new window...Saccharomyces cerevisiae. Yeast, 21 (8):661-70. https://doi.org/10.1002/yea.1130 (Link opens in a new window) PMID... cerevisiae. Microb Cell Fact, 12 :96. https://doi.org/10.1186/1475-2859-12-96 (Link opens in a new window...

  15. Microbiology Resources

    Type
    Collection
    ...these model organisms. See below for plasmid kits and other resources relevant to these organisms, or use ...depositors are making this possible in a range of organisms. Find CRISPR tools for use in genome editing of...extensive fluorescent protein plasmid collection is organized by application and by color. External Resources...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...

  16. Structural Genomics Consortium Plasmids

    Type
    Collection
    ...Related Resources SGC: http://www.thesgc.org/ SGC Toronto: https://www.thesgc.org/scientists/groups/toronto...Structural Genomics Consortium is a not-for-profit organization that aims to determine the three dimensional...www.sgc.ox.ac.uk/ Protein Database (PDB): http://www.pdb.org Request Plasmids To order, Login or Register ...

  17. Distribution to Industry

    Type
    Collection
    ...account and affiliate it with your organization. If your organization has not ordered from Addgene in the...fax, or email. Pricing Addgene is a nonprofit organization dedicated to providing the scientific community...through your user account or by email to mta@addgene.org . After we review and approve the MTA documents...

  18. Fluorescent Protein Guide: Empty Backbones

    Type
    Collection
    ... Fluorescent Protein Fusions (Organized by Expression Species) Organism/Expression System Plasmids Mammalian...proteins with your gene of interest. These are organized By Color of the fluorescent protein and By Expression...Empty Backbones for Fluorescent Protein Fusions (Organized by Color) For each table: Excitation and Emission...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource! Jump to Top...

  19. CRISPR Plasmids - Tagging

    Type
    Collection
    ...locus Jorgensen Lab SapTrap CRISPR/Cas Toolkit SapTrap is a modular toolkit from Erik Jorgensen's lab for... and a prebuilt Unc-32::GFP targeting vector. Jorgensen Lab SapTrap CRISPR/Cas Toolkit Kanemaki Lab Auxin-Inducible...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...

  20. Adeno-associated virus (AAV) Guide

    Type
    Guide
    ...AAV vectors . Microorganisms, 12 (2), 384. https://doi.org/10.3390/microorganisms12020384 (Link opens in...genes. rAAV episomes can develop chromatin-like organization and persist in non-dividing cells for a period...different serotype(s) for transduction of a given organ. Tissue Serotype Central nervous system AAV1, AAV2...administration, distributing widely throughout the organism to target large or diffused biological structures... of systemic rAAV vectors that can target whole organs or specific cell types of the CNS (e.g. AAV-PHP.eB...Packaged-on-Request AAV Preps In-Stock AAV Preps Resources on addgene.org AAV Plasmids Available at Addgene Viral Vectors... . Molecular Therapy, 10 (2), 302–317. https://doi.org/10.1016/j.ymthe.2004.05.024 (Link opens in a new...
Showing: 21 - 40 of 947 results