...send you daily updates with relevant literature. Organizing the literature As a scientist, you will begin...will ever read. So, you need to come up with an organization system that works for you. I have a folder where...’m still in the dark ages with respect to my organization strategy. There are a number of software options...another option that may help solve your literature organization issues – F1000 just announced the launch of ....  Give your favorite searching, reading and organization tips in the comments below. Additionally, read...

...next R01 is due. But what about organizing yourself and your lab? Organizing your lab notebooks, protocols...you think! Here are three tips to help you get organized and boost your productivity in 2014. Tip 1: Use...and no closer to starting your experiments. Get organized now! Start by gathering that pile of lab notebooks...your tasks on the go Never miss a deadline again. Organize you experiments, keep track of upcoming grant ... clippings, photographs, and file attachments. Organize your notes into notebooks, create checklists that...spreadsheets with lab members Now that you’ve organized your protocols, wouldn’t it be great if you could...reagent storage locations, such as the list used to organize your cryo freezer. No more issues deciphering ...

... model organism blog posts: Five Popular Model Organisms, Part 1 Five Popular Model Organisms, Part 2...diverse organisms. Methods for genetic manipulation still need to be developed for each organism, but researchers...and share all of these new organisms continues to be challenging. Organism specific viral vector systems...programs via Addgene. New organisms for genotype to phenotype studies New organismal systems described at ...and shared for many different organisms. Do you have a favorite organism you study in the lab? Let us ... work but never get to actually manipulate the organism they study (unless if you count normal human interaction...spiny purple sea urchin. It’s not a standard model organism, but it was easy to harvest eggs and sperm, mix...

...transcriptional machinery from a particular organism or group of organisms. Meaning, if a plasmid in intended ...abbreviated to A, T, C, and G respectively) that are organized into a double stranded helix. The order of these...the instructions to make every protein within an organism. Proteins are made up of amino acids. Each amino...can drive gene expression in a wide variety of organisms, including plants, worms, mice, and even cultured...when trying to sequence an entire plasmid or an organism’s genome. One Sanger sequencing reaction would ...

...these principles apply to using CRISPR in other organisms. First, consider the genetic manipulation that...carry out your experiment in your particular model organism. There may not be a perfect plasmid for your specific...disrupt gene function in a particular cell type or organism without a specific preferred mutation Cas9 (or...sequence and design your gRNA. Know your cell line/organism and genomic sequence When possible, you should...may vary depending on the specific cell line or organism, which may affect the observed efficiency of CRISPR...

...cycle. rAAV episomes can develop chromatin-like organization and persist in non-dividing cells for a period...optimal serotype(s) for transduction of a given organ. Tissue Optimal Serotype CNS AAV1, AAV2, AAV4, AAV5...

...that both KORD and hM3Dq can be used in the same organism to allow bidirectional control of neuronal activity...Knauss S, von Schimmelmann M, During MJ, Lignani G, Schorge S, Young D, Kullmann DM, Lieb A (2019). Olanzapine...

...myeloma cells (immortalized B cells). The genetic reorganization that creates variety in the antibody allowing...the variable region goes through a randomized reorganization process, creating a unique binding site. This...

...knock out target genes in various cell types and organisms, but modifications to various Cas enzymes have...be added to detect the presence of infectious organisms (like SARS-CoV-2 ) and genetic mutations. Similar...

...promoter region controls when and where in the organism your gene of interest is expressed. Summary Promoters...

... sure what primer you need? Email us at help@addgene.org Addgene has used a number of primers for sanger...

...Spring Harbor, 1997. Available online: Genetic Organization and Genetic & Structural Features . In vivo ...

...and study certain behavioral responses in model organisms like mice, zebrafish, and Drosophila. These tools...

...been established as a powerful vertebrate model organism for the study of genetics and developmental biology...Multicolor fluorescent labeling in zebrafish Whole organism lineage tracing by combinatorial and cumulative... previously resistant cell types. CreLite - George Eisenhoffer Lab. An optogenetically controlled ...screening fluorescent protein fusion expression in organisms or cells in which an exogenous mRNA can be injected...assembly for the zebrafish genome. Zebrafish Model Organism Database (ZFIN) - A curated database of genetic... International Zebrafish Society (IZFS) - An organization that promotes and advocates for zebrafish research...aims to increase the use of zebrafish as a model organism and to foster advanced training of young scientists...

...PMCID: PMC3531189. Mangone, Marco, et al. "UTRome. org: a platform for 3′ UTR biology in C. elegans." Nucleic...source project that is creating the “first virtual organism in a computer.” This project has produced various..., we have the entire SapTrap toolkit from the Jorgensen lab. SapTrap is a reagent toolkit and plasmid ...repair template of your choosing (Schwartz and Jorgensen, 2016). Using SapTrap, this CRISPR plasmid can...published in a microPublication (Schwartz and Jorgensen, 2018).  These are just some examples of worm ...PMC2238901. Schwartz, Matthew L., and Erik M. Jorgensen. "SapTrap, a toolkit for high-throughput CRISPR...Central PMCID: PMC4905529.  Schwartz, M. L., & Jorgensen, E. M. "SapTrap vectors for introducing point ...

...Scaffold for inorganic phosphate biosensor Development of a Reagentless Biosensor for Inorganic Phosphate...biosensors can be introduced into cells, tissues, or organisms for long-term imaging and can be designed to specifically...specifically localize to cellular compartments or organelles. Addgene’s database includes many fluorescent.... in vivo , cytoplasmic vs. extracellular vs. organelle-localized). Browse or search the tables below ...and In Vivo Imaging of Ca Dynamics in High-Ca Organelles. Cell Chem Biol. 2016 Jun 1. pii: S2451-9456(...sensors (green, red or blue/green) Imaging intraorganellar Ca(2+) at subcellular resolution using CEPIA...Genetically Encoded FRET Sensor for Cytosolic and Intraorganelle Zn(2+) Imaging. ACS Chem Biol. 2015 Sep 18;...

... “the worm”) is widely used as a genetic model organism due to their rapid life cycle (3 days from egg...Lab website . SapTrap CRISPR/Cas Toolkit - Erik Jorgensen Lab. A collection of plasmids that streamlines... Transgenesis miniMos Transgenesis Kit - Erik Jorgensen Lab. This kit contains 29 plasmids used to generate... related miniMos plasmids can be found on the Jorgensen Lab page. More information on miniMos and MosSCI...methods to engineer the genome of the genetic model organism C. elegans. Return to top Do you have suggestions...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...

...RF1 function removed George Church 48999 C321 all TAG sites changes to UAG George Church 49018 C321.ΔA.exp...pairs of synthetase and tRNA that work for one organism may not work for another. The orthogonal synthetase...removed, MutS restored so decreased mutation rate George Church 68306 C321.ΔA all TAG sites changes to UAG..., RF1 function removed, improved doubling time. George Church 98564 C321.Ub-UAG-sfGFP all TAG sites changes...function removed, with Ubiquitin-UAG-sfGFP reporter George Church 98565 C321.ΔClpS.Ub-UAG-sfGFP all TAG sites... inactivated, with Ubiquitin-UAG-sfGFP reporter George Church 174513 Syn61 No TCG, TCA, or TAG codons ...codons. Deletion of serT, serU, prfA, and recA genes George Church 192872 rEcoli XpS All 321 UAG codons changed...

... structures give the cell its shape, organize cellular organelles, and are necessary for movement and ... Plasmid Collections Fluorescent Proteins Organelle Markers Fluorescent Proteins: Subcellular Localization... fusion proteins that are targeted to various organelles or subcellular structures. You should fuse your...General Structures The cell contains a variety of organelles and subcellular structures each with their own...table below to find markers of general cellular organelles and structures. ID Plasmid Structure Targeting...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...

...these model organisms. See below for plasmid kits and other resources relevant to these organisms, or use ...depositors are making this possible in a range of organisms. Find CRISPR tools for use in genome editing of...extensive fluorescent protein plasmid collection is organized by application and by color. External Resources...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...