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Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 404 results for: pho;

Showing: 21 - 40 of 404 results

  1. Bacterial Expression Systems

    Type
    Collection
    ...collection of FPs, including photoactivatable, photoconvertible, and photoswitchable proteins, organized by ...when a donor fluorophore is excited, it can transfer its energy to an acceptor fluorophore if they are ...Fusion Proteins — Making the Right Connection Photoactivatable Fluorescent Proteins Fluorescent Protein Timers...fluorescence both from the donor and the acceptor fluorophore by only exciting the donor. In BiFC, proteins...Inducible Promoters Repressible Promoters Synthetic Photobiology: Optogenetics for E. coli Return to top Reporter...

  2. Recombinases AAV Preps

    Type
    Collection
    ...these molecular tools. Cre AAV ID Name Promoter Fluorophore/Tag Serotype(s) PI 105551 pENN.AAV.CamKII.HI....AAV.TBG.PI.Cre.rBGe TBG none 8 Wilson Dre AAV ID Name Promoter Fluorophore Serotype(s) PI 50363 AAV phSyn1(S)-DreO-bGHpA...Syn none 5, rg* Zeng Flpo AAV ID Name Promoter Fluorophore Serotype(s) PI 51669 AAV phSyn1(S)-FlpO-bGHpA...Light-Inducible Recombinases ID Name Promoter Fluorophore Serotype(s) PI 140135 pAAV-EF1a-iCreV EF1a none...none 1, PHPeB Zeng VCre AAV ID Name Promoter Fluorophore/Tag Serotype(s) PI 55638 pAAV-EF1a-vCre EF1a ...

  3. Luciferase Plasmid Collection

    Type
    Collection
    ... of photons. Its robust signal, sensitivity, wide dynamic range, and resistance to photobleaching have...Firefly Creation of N-terminal Firefly fusions in Drosopholia Michael Rosbash 128046 pGL3basic luciferase and... from the luciferase reaction to an acceptor fluorophore can provide more flexibility in emission colors...BRET energy donor and HaloTag labeled with a fluorophore as the acceptor. LumiFluors: EGFP-NanoLuc ( GpNLuc... sensor created by fusing NanoLuc® and Venus fluorophore to Troponin C Ca 2+ binding domain. Luminopsins...

  4. Zhang Lab CRISPR Page

    Type
    Collection
    ...to enable the rapid ligation of annealed and phosphorylated oligos designed based on the target site sequence...

  5. Fluorescent Protein Guide: Empty Backbones

    Type
    Collection
    ...Near-Infrared Long Stokes Shift Photoactivatable Photoconvertible Photoswitchable Timers Blue/UV Protein Excitation...LSSmKate2-C1 - Mammalian Expression Jump to Top Photoactivatable (e.g. off to on) Excitation and Emission wavelengths...HisB-PAiRFP2 - Bacterial Expression Jump to Top Photoconvertible (e.g. green to red) Protein Excitation (nm...pBAD-PSmOrange2 - Bacterial Expression Jump to Top Photoswitchable (e.g. off to on to off) Protein Excitation ...

  6. mTOR Pathway

    Type
    Collection
    ...PIK3R2 PIK3R3 PIK3R4 PIK3R5 PIK3R6 Phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic and regulatory...AKT1S1; AKT1 substrate 1 (proline rich) PTEN Phosphatase and tensin homolog Rag RRAGA RRAGB RRAGC RRAGD...Protor Also known as PRR5; proline rich 5 PTEN Phosphatase and tensin homolog Rictor RPTOR independent companion...

  7. p53 Pathway

    Type
    Collection
    ...deprivation. p53 is activated through both phosphorylation and acetylation, which disrupt its interaction...ligase MDMX p53 regulator; also known as MDM4 Noxa Phorbol-12-myristate-13-acetate-induced protein 1; also... 1 (RCHY1); E3 ubiquitin protein ligase PTEN Phosphatase and tensin homolog PUMA BCL2 binding component...

  8. Genomic Deletions in Mammalian Cell Lines

    Type
    Collection
    ...2.1 may be omitted. CRISPR Cloning Anneal and phosphorylate oligos. Resuspend oligos at a concentration ...Polynucleotide Kinase (PNK) (10,000 U/ml). NOTE: Phosphorylated oligos may be ordered instead. For this approach...

  9. Brzezinski Lab CRISPR Collection

    Type
    Collection
    ...followed by a quick ligation of annealed and phosphorylated oligonucleotides containing the guide target...Prdm1 and Vsx2 enhancers in the retina alters photoreceptor and bipolar cell fate specification, yet differs...

  10. Antibody Production

    Type
    Collection
    ...are initially titered by spectrophotometry using a NanoDrop spectrophotometer. If needed, the sample is...centrifugal columns. The final formulation buffer is phosphate-buffered saline containing 1 mM sodium azide. ...

  11. Zebrafish Plasmid Collection

    Type
    Collection
    ...transposons, Cre-mediated site-specific recombination, morpholinos, zinc finger nucleases, TALENS, and CRISPR/Cas9...of gene function in zebrafish - Julian Lewis Lab PhOTO Zebrafish: A Transgenic Resource for In Vivo Lineage...consisting of plasmids with four promoters, six fluorophores, and empty vectors designed to streamline the...

  12. Brain Initiative Collection

    Type
    Collection
    ...Cation channelrhodopsin ChrimsonR fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal...parapinopsin driven by the CAG promoter. Used for photoswitchable control of inhibitory GPCR signaling cascades...parapinopsin driven by the Ef1a promoter. Used for photoswitchable control of inhibitory GPCR signaling cascades...

  13. Trimmer Lab NeuroMab Collection

    Type
    Collection
    ... Ca2+ channel phosphorylation site pS1928 [L132/18R] Cav1.2 Ca2+ channel phosphorylation site pS1928 Rat...Anti-Kv1.2 pS440/pS441 phosphospecific [L64/32] Kv1.2 pS440/pS441 phosphospecific Human Mouse IgG2a 190291...

  14. CRISPR Guide

    Type
    Collection
    ...SpCas9-HF1 - disrupt Cas9’s interactions with DNA phosphate backbone HypaCas9 - increase Cas9 proofreading...fusing the anti-CRISPR protein AcrIIA4 with the photosensitive LOV2 domain . Several labs have also controlled...: Epigenetics Visualizing Genomic Loci Using Fluorophores CRISPR imaging has numerous advantages, including...11: Overview of visualizing genomic loci with fluorophores using CRISPR Browse Plasmids: Visualize Purify...Yoshimi, K., Okuzaki, Y., Gee, P., Kunihiro, Y., Sonpho, E., Xu, H., Sasakawa, N., Naito, Y., Nakada, S...

  15. Michael Davidson Fluorescent Protein Collection

    Type
    Collection
    ...mEmeralds Reds Blues Cyans Oranges Greens Yellows Photoactivatables Empty Backbones Plasmid Description Industry...Industry Yellows Plasmid Description Industry Photoactivatables Plasmid Description Industry Do you have suggestions...

  16. Depositor Collections

    Type
    Collection
    ...Reagents for improved expression of recombinant phosphoproteins - Rinehart FreeGenes Project Open Enzyme Collection...

  17. Synthetic Biology - Overview

    Type
    Collection
    ...Optogenetics Pertz Lab cpFRET Kit Rinehart Recombinant Phosphoprotein Kit S. cerevisiae Advanced Gateway Destination...

  18. Worm Expression Resources

    Type
    Collection
    ...uses purified Cas9, such as that expressed from pHO4d-Cas9 from Michael Nonet. Protocol can be found at...and GFP fusion vectors. C. elegans optimized fluorophores - Andrew Fire Lab. Available with and without...

  19. Allen Institute for Cell Science Plasmid Collection

    Type
    Collection
    ...Sialyltransferase 1 Golgi 109122 NPM1-mEGFP AICSDP-50 mEGFP Nucleophosmin Nucleolus (granular component) 109120 GJA1-mEGFP...z-disks 124608 NPM1-mTagRFP-T AICSDP-69 mTagRFP-T Nucleophosmin Nucleolus (granular component) 133963 UBTF-HaloTag...

  20. Kazuhiro Oka Lentiviral Vectors

    Type
    Collection
    ...72268 xpress gene of interest from the PGK (phosphoglycerate kinase I) promoter pCDH-CMV4 72284 Express...
Showing: 21 - 40 of 404 results