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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 399 results for: AGA

Showing: 381 - 399 of 399 results

  1. Protocol - How to Inoculate a Bacterial Culture

    Type
    Protocol
    ... on your LB agar plates are not fresh, you should streak your bacteria onto a new LB agar plate before...like... Streaking and Isolating Bacteria on an LB Agar Plate Creating Bacterial Glycerol Stocks for Long-term...commonly used to culture bacteria in the lab. LB agar plates are frequently used to isolate individual...2 O to 400 mL Note: If your lab has pre-mixed LB agar powder, use the suggested amount, instead of the...or toothpick, select a single colony from your LB agar plate . Drop the tip or toothpick into the liquid...

  2. Plasmid Cloning by PCR (with Protocols)

    Type
    Protocol
    ...final Forward Primer sequence of 5'-TAAGCAGAATTCATGTGGCATATCTCGAAGTAC-3'. For the Reverse Primer, the design...final Reverse Primer sequence of 5’-TGCTTAGCGGCCGCTCAGTACTTCGAGATATGCCA-3’. Experimental Procedure Run PCR... by Gel Purification Run your digest DNA on an agarose gel and conduct a gel purification to isolate the...you used for the cloning. Run your digest on an agarose gel . You should see two bands, one the size of...

  3. Antibody Validation Using the Indirect ELISA Method

    Type
    Protocol
    ...protocol describes how to run an indirect ELISA against a purified antigen to demonstrate the expected ...indirect Enzyme-Linked ImmunoSorbent Assay (ELISA) against a purified antigen to validate an antibody. This... the antigen and how to probe with an antibody against the target to show a dose response. Sharing speeds...minutes. Absorbance was read at 450 nm and plotted against the micrograms of antigen loaded. Last reviewed...

  4. Protocol - How to Create a Bacterial Glycerol Stock

    Type
    Protocol
    ...competent cells and retransform. Bacteria on an LB agar plate can be stored at 4°C for a few weeks. However...glycerol stock unthaw! Streak the bacteria onto an LB agar plate . Grow your bacteria overnight at the appropriate...glycerol stock on dry ice while streaking onto LB agar will prevent it from thawing completely and will...

  5. Kit Free RNA Extraction

    Type
    Protocol
    ...You may also like... Kit-Free DNA Purification Agarose Gel Purification Molecular Biology Reference Introduction...using a spectrophotometer (such as a Nanodrop), agarose gel, or bioanalyzer. For more information on nucleic...

  7. Immunocytochemistry

    Type
    Protocol
    ...staining cells in culture with a primary antibody against a target protein and a fluorescent secondary antibody...labeling cells in culture with a primary antibody against a target protein and a fluorescent secondary antibody...

  10. Protocol - How to Design Primers

    Type
    Protocol
    ...Polymerase Chain Reaction Plasmid Cloning by PCR Agarose Gel Electrophoresis Primer Design for PCR Oligonucleotide...

  13. General Transfection

    Type
    Protocol
    ...quality plasmid DNA, the plasmid should also be propagated in an endonuclease negative E. coli strain such...

  15. Gibson Assembly Protocol

    Type
    Protocol
    ...Generate DNA segments by PCR. Run PCR product on an agarose gel to check for size and yield. If there are ...

  16. Lentivirus Production

    Type
    Protocol
    ...quality plasmid DNA, the plasmids should also be propagated in an endonuclease negative E. coli strain such...

  19. Lentivirus ddPCR Titration

    Type
    Protocol
    ...modified for other targets. Primers and a probe against the cellular ribonuclease P/MRP 30 kDa subunit ...
Showing: 381 - 399 of 399 results