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  2. Immunocytochemistry

    Type
    Protocol
    ...staining cells in culture with a primary antibody against a target protein and a fluorescent secondary antibody...labeling cells in culture with a primary antibody against a target protein and a fluorescent secondary antibody...

  5. Protocol - How to Design Primers

    Type
    Protocol
    ...Polymerase Chain Reaction Plasmid Cloning by PCR Agarose Gel Electrophoresis Primer Design for PCR Oligonucleotide...

  8. General Transfection

    Type
    Protocol
    ...quality plasmid DNA, the plasmid should also be propagated in an endonuclease negative E. coli strain such...

  10. Gibson Assembly Protocol

    Type
    Protocol
    ...Generate DNA segments by PCR. Run PCR product on an agarose gel to check for size and yield. If there are ...

  11. Lentivirus Production

    Type
    Protocol
    ...quality plasmid DNA, the plasmids should also be propagated in an endonuclease negative E. coli strain such...

  14. Lentivirus ddPCR Titration

    Type
    Protocol
    ...modified for other targets. Primers and a probe against the cellular ribonuclease P/MRP 30 kDa subunit ...
Showing: 381 - 394 of 394 results