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This post discusses the core replication and resistance features of yeast vectors and explains how these plasmids differ from their bacterial counterparts.

Addgene's zebrafish resources: Find plasmids for use in zebrafish.

Addgene's collection of plasmids for the p53 pathway. p53 is a transcription factor and tumor suppressor activated by cellular stress.

Addgene data on CRISPR and synthetic biology plasmids deposited to the repository and distributed around the world.

If you need to image more than one protein in your sample, multiplex immunofluorescence is an important skill to master!

A review of statistical testing basics and the most common parametric comparison tests.

A deep dive into qPCR with a focus on analysis, chemistries, and bench tips.

Dr. Le Cong, first author on a seminal CRISPR article from Dr. Feng Zhang's lab, answers FAQs about the genome editing technology.

A description and video demonstration of the new FLIES plasmid.

Sequence and ligation independent cloning (SLIC) lacks the strict requirements of many standard cloning techniques. Learn how to use SLIC in this post.

Adeno-associated Virus (AAV) is used extensively for in-vivo transgene expression. Learn how to use AAV in this post.

Working across teams can lead to break downs in communication. Learn how removing jargon, adding context, and creating a lexicon document can improve cross-team communication.

There are many tools available for studying the microbiome. Learn about plasmid tools for engineering gut microbes, detection, and synthetic biology.

Congratulations to Paula Videira, Michael McMurray, & Richard Gronostajski who each are being awarded free plasmids form Addgene. Read on to learn how these researchers plan to use plasmids for research on diseases of glycosylation, arginine mutagenesis, & neural development.

Learn all about plasmids for protein degradation, endosome biosensing, studying the epigenome, and controlling gene expression in yeast.

Learn how to measure your fluorescent protein's photostability and get the most out of your next fluorescent imaging experiment.

Use CRISPRainbow to label multiple genome sites with fluorescent proteins of various colors.

It's important to determine how much functional virus you have a in your prep before starting a transduction. Learn how to titer your lentivirus here.

Golden gate cloning can be used to efficiently and inexpensively clone multiple inserts into a single vector. Learn the basics of golden gate here.

Tips for writing your scientific manuscript, including how to search for and organize papers.