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Learn how to determine the practical brightness of your fluorescent protein in your specific experimental setup.

R bodies are tiny, tunable molecule machines that can be used to break membranes. Learn all about them and their potential uses here.

Blue-white screening is a well loved technique that allows scientists to quickly identify recombinant clones for further analysis.

Advice on starting a biotech company from how to take an idea developed during graduate school and turn it into a startup.

This post discusses the core replication and resistance features of yeast vectors and explains how these plasmids differ from their bacterial counterparts.

Addgene data on CRISPR and synthetic biology plasmids deposited to the repository and distributed around the world.

If you need to image more than one protein in your sample, multiplex immunofluorescence is an important skill to master!

A review of statistical testing basics and the most common parametric comparison tests.

A deep dive into qPCR with a focus on analysis, chemistries, and bench tips.

Dr. Le Cong, first author on a seminal CRISPR article from Dr. Feng Zhang's lab, answers FAQs about the genome editing technology.

A description and video demonstration of the new FLIES plasmid.

Sequence and ligation independent cloning (SLIC) lacks the strict requirements of many standard cloning techniques. Learn how to use SLIC in this post.

Adeno-associated Virus (AAV) is used extensively for in-vivo transgene expression. Learn how to use AAV in this post.

Working across teams can lead to break downs in communication. Learn how removing jargon, adding context, and creating a lexicon document can improve cross-team communication.

There are many tools available for studying the microbiome. Learn about plasmid tools for engineering gut microbes, detection, and synthetic biology.

Congratulations to Paula Videira, Michael McMurray, & Richard Gronostajski who each are being awarded free plasmids form Addgene. Read on to learn how these researchers plan to use plasmids for research on diseases of glycosylation, arginine mutagenesis, & neural development.

Learn all about plasmids for protein degradation, endosome biosensing, studying the epigenome, and controlling gene expression in yeast.

Learn how to measure your fluorescent protein's photostability and get the most out of your next fluorescent imaging experiment.

Use CRISPRainbow to label multiple genome sites with fluorescent proteins of various colors.

It's important to determine how much functional virus you have a in your prep before starting a transduction. Learn how to titer your lentivirus here.