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We narrowed to 772 results for: Dos

Showing: 381 - 400 of 772 results

  1. R Bodies: Membrane-Rupturing Microscopic Tools

    Type
    Blog Post
    .... Unlike cytoskeletal polymers, however, R bodies do not exert force by dynamically rearranging into different...bodies can be found at Addgene here. What can R bodies do for you? R bodies are robust linear actuators that...

  4. Plasmids 101: Yeast Vectors

    Type
    Blog Post
    ... the bacterial elements previously described. Why do scientists use yeast vectors? Yeast are eukaryotes... Plasmids for use in S. pombe, on the other hand, do not require a well defined ORI. Instead, the size...

  5. Zebrafish Plasmid Collection

    Type
    Collection
    ... single cell genomics in zebrafish. Return to top Do you have suggestions for other plasmids that should...

  6. p53 Pathway

    Type
    Collection
    ...Biology. 2010 Feb;2(2):a001107. PMC PMID: 20182618 . Do you have suggestions for other plasmids that should...

  7. Fluorescent Protein Guide: FRET

    Type
    Collection
    ... FRET) with pmVenus(L68V)-mTurquoise2 Back to Top Do you have suggestions for other plasmids that should...

  8. Antibodies 101: Multiplex Immunofluorescence

    Type
    Blog Post
    ...’ll need to align all these images once taken. To do so, you will need to include a marker like the Hoechst...needs, your sample, and targets, you might decide to do simultaneous IF instead (Figure 1B). To label all...important consideration when deciding whether you can do simultaneous or sequential labeling is what conjugated...

  9. Deep Dive: Statistical Tests (Comparisons)

    Type
    Blog Post
    ...exciting trends in your experimental groups, but how do you determine if they deserve that oh-so coveted ...can be run on these tests. All sample comparisons Do you really need to know every pairwise comparison...mathematician to select the right statistical test, just do your homework and happy testing!   References Wissing...

  10. How to Design Your gRNA for CRISPR Genome Editing

    Type
    Blog Post
    ...use, of course, depends on what you are trying to do – there’s no “best” tool among them. While this seems...gRNA depends an awful lot on what you are trying to do: gene knockout, a specific base edit, or modulation...importance in design – an optimized sequence will do little if it is in the wrong place, but because the...

  11. Deep Dive: qPCR

    Type
    Blog Post
    ... for your cell lines and/or tissue types.  Please do note my oh-so-subtle use of reference genes, plural...reference genes - and it is well worth your time to do one (Biassoni, 2014). At the bench As a highly sensitive...you from understanding how the analyses work - you do need to make sure the assumptions in your dataset...end of your analysis process. You’ll still need to do statistical analysis to determine if there are any...

  12. CRISPR/Cas9 FAQs Answered!

    Type
    Blog Post
    ...sequence starts with a 'G', you naturally have one and do not need to add an additional 'G'. Q4: Where are ...) template, you could then: Confirm your HR by doing Restriction Fragment Length Polymorphism (RFLP) ...DMSO or without 5% DMSO. My genomic PCR didn’t work, do you have any suggestions? A14: The Zhang lab recommends...you used in the Science paper (Cong et al., 2013) do you have any suggestions? A15: To date, I have never...

  14. Antibodies 101: Fab Fragments

    Type
    Blog Post
    ...Imagine an antibody. Do you immediately visualize a Y-shaped protein reminiscent of the Addgene mascot...sure to think about what pieces of the antibody you do and don’t need, and how the protein will interact...

  15. Antibodies 101: Stripping and Reprobing Western Blots

    Type
    Blog Post
    ...during your western and your results look… funky? Do you need to start a whole new blot? Never fear: membrane...directly to buffer without a re-wetting step. Important: do not dry your membrane with antibodies on it if you...

  16. Choosing the B(right)est Fluorescent Protein: Photostability

    Type
    Blog Post
    ...fluorescent proteins or localized fusion proteins and do not require dedicated plasmids or constructs. It ...allow for a well-controlled environment, but they do not mimic the natural situation. Measuring photostability...

  17. CRISPRainbow and Genome Visualization

    Type
    Blog Post
    ...ability to target almost every genomic locus, and to do so in vivo. Catalytically dead Cas9 (dCas9) cannot...CRISPRainbow requires very short, 11-mer gRNAs, which do not induce genome editing. If used with catalytically...

  19. Viral Vectors 101: Virus Safety

    Type
    Blog Post
    ...virus to mice, part of your preparation should be doing your best to be well-rested and alert the day of...procedure. Mice move much more quickly than cell cultures do, and tired researchers are accident-prone researchers...

  20. Viral Vectors 101: An Introduction to AAV

    Type
    Blog Post
    ... cell and animal models. AAVs are non-pathogenic, do not integrate into the host genome, and can be specifically...several years (Wonjo et al., 2013). Remember that AAVs do not integrate into the host genome and therefore ...
Showing: 381 - 400 of 772 results