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We narrowed to 768 results for: Dos

Showing: 381 - 400 of 768 results

  1. CRISPR/Cas9 FAQs Answered!

    Type
    Blog Post
    ...sequence starts with a 'G', you naturally have one and do not need to add an additional 'G'. Q4: Where are ...) template, you could then: Confirm your HR by doing Restriction Fragment Length Polymorphism (RFLP) ...DMSO or without 5% DMSO. My genomic PCR didn’t work, do you have any suggestions? A14: The Zhang lab recommends...you used in the Science paper (Cong et al., 2013) do you have any suggestions? A15: To date, I have never...

  3. Chemogenetics Guide

    Type
    Guide
    ...coupled receptors? Read the GPCRs: How Do They Work and How Do We Study Them? blog! Studies of GPCRs led...are engineered to respond to small molecules that do not affect endogenous signaling, therefore allowing...DCZ seem to have minimal off-target activity, and do not seem to have the same back-metabolism issues ... consider: Chemogenetic activation or inhibition. Do you want to turn ON or turn OFF neurons in your experiment...high potency, which is potentially important for dosing in animal studies, made them attractive targets...

  4. Ras Pathway

    Type
    Collection
    ...Cancer. 2003 Jan;3(1):11-22. PubMed PMID: 12509763 . Do you have suggestions for other plasmids that should...

  5. TALEN Plasmids and Kits

    Type
    Collection
    ...specific repetitive DNA sequences in human chromosomes Do you have suggestions for other plasmids that should...

  6. Lentivirus Plasmids

    Type
    Collection
    ...more versions of this plasmid. Root Return to Top Do you have suggestions for other plasmids that should...

  7. Viral Vectors 101: Virus Safety

    Type
    Blog Post
    ...virus to mice, part of your preparation should be doing your best to be well-rested and alert the day of...procedure. Mice move much more quickly than cell cultures do, and tired researchers are accident-prone researchers...

  8. Viral Vectors 101: An Introduction to AAV

    Type
    Blog Post
    ... cell and animal models. AAVs are non-pathogenic, do not integrate into the host genome, and can be specifically...several years (Wonjo et al., 2013). Remember that AAVs do not integrate into the host genome and therefore ...

  9. Transferable Skills Guide: Cross-team Communication

    Type
    Blog Post
    ... only problematic word. How many different things do you simply refer to as a “tube”? We took steps towards...lab or collaborators will not have the context you do, which means you’ll need to provide that context ...

  10. Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)

    Type
    Blog Post
    ...different epitopes on the target protein, so they do not compete with each other. This antibody is also...interest that is present. Using a Standard Curve To do that calculation, you’ll need to include a standard...

  11. How to Prepare for an Industry Interview

    Type
    Blog Post
    ...had an industry interview before. What should you do to get ready for this interview? Maybe the above ...the same boat, but there are a few things you can do to make the experience less stressful. First, you...

  13. Antibodies 101: Fab Fragments

    Type
    Blog Post
    ...Imagine an antibody. Do you immediately visualize a Y-shaped protein reminiscent of the Addgene mascot...sure to think about what pieces of the antibody you do and don’t need, and how the protein will interact...

  14. Antibodies 101: Stripping and Reprobing Western Blots

    Type
    Blog Post
    ...during your western and your results look… funky? Do you need to start a whole new blot? Never fear: membrane...directly to buffer without a re-wetting step. Important: do not dry your membrane with antibodies on it if you...

  15. Hot Plasmids April 2018 - Protein Degradation, Nanoscopy, FIRE-Cas9, and Yeast Expression Tools

    Type
    Blog Post
    ...inducer. Since induction via estradiol and progesterone do not affect each other, these systems can be used ...random variation, or noise, in reporter expression. To do so, one places reporter expression under the control... et al. Cell. 2017. PubMed PMID: 29153837. Endosomal pH sensors for STED nanoscopy Article contributed...contributed by Eric J. Perkins Listen to Endosomal pH Sensors podcast segment The first Addgene plasmid deposit... biosensors for super-resolution microscopy.” Endosomes are historically difficult to study in living ...met some of the criteria necessary for studying endosomes, the Hell lab designed their super-resolution ... meet all of them. These probes had to target endosomes, be exceptionally bright (which is necessary for...

  16. Choosing the B(right)est Fluorescent Protein: Photostability

    Type
    Blog Post
    ...fluorescent proteins or localized fusion proteins and do not require dedicated plasmids or constructs. It ...allow for a well-controlled environment, but they do not mimic the natural situation. Measuring photostability...

  17. CRISPRainbow and Genome Visualization

    Type
    Blog Post
    ...ability to target almost every genomic locus, and to do so in vivo. Catalytically dead Cas9 (dCas9) cannot...CRISPRainbow requires very short, 11-mer gRNAs, which do not induce genome editing. If used with catalytically...

  18. Tips for Titering Your Lentiviral Preps

    Type
    Blog Post
    ... all have their benefits and drawbacks. Labs that do not routinely produce virus may want to choose more...Additional Resources on Addgene.org Learn How We Do Viral Production at Addgene Read Our Lentiviral Guide...

  19. Plasmids 101: Golden Gate Cloning

    Type
    Blog Post
    ...fragments. When designed correctly, the recognition sites do not appear in the final construct, allowing for precise...these sites are eliminated by digestion/ligation and do not appear in the final construct. The destination...
Showing: 381 - 400 of 768 results