...plant biologists, Addgene has not one, not two, but three CRISPR MoClo kits for plants that work for both ...

...of the instrumentation A flow cytometer combines three systems to analyze single cells from a mixture (...

...antibody Fab’ fragments: An overview and comparison of three of the most versatile biosensor biorecognition elements...

... single, thin plane within a relatively thicker three dimensional sample (see Figure 1); similar to an...

...Behavioral Optogenetics can be broken down broadly into three main steps: 1) Selection and expression of your...

...amounts of high quality DNA. Figure 1 illustrates the three grades of DNA you may need based on their relative...

...Dedifferentiation, transdifferentiation and reprogramming: three routes to regeneration. Nat Rev Mol Cell Biol, 2011...

...Figure 1). It can therefore be helpful to try all three when looking to label an intracellular target.   ...

...for automated rational design. The first of only three such databases, EENdb (published 1 Jan 2013, no ...

...In order to produce lentiviral vectors, you need three (or four, for third generation) plasmids: Transfer...VSV-G Safety Safe; replication incompetent by using three separate plasmids encoding the necessary viral genes...replication incompetent by using four plasmids instead of three and eliminates the requirement for Tat. Always includes...lentiviral production. All lentiviral plasmids (three or four) are transfected into HEK293T cells using...

...replication and viral packaging. The Cap region encodes three structural capsid proteins or viral proteins (VP1...different plasmid. In order to produce rAAV vectors, three plasmids are needed: Transfer plasmid (also known...’s Molecular Biology Reference . Production The three production plasmids (transfer, packaging, and helper...Rep52, and Rep40. Cap in trans The cap gene encodes three AAV viral capsid proteins VP1, VP2, and VP3, which...

...-targeting gRNA and a dCas9 fusion protein with three red fluorescent tags in U2OS cells. Upon light activation...

...QCascade DNA-targeting module with the crRNA, (3) three transposase proteins, and (4) the donor DNA. By ...

...order to produce gamma-retroviral vectors, you need three plasmids (Figure 2): Transfer plasmid — contains..., similar to lentiviral packaging methods . The three plasmids described above (envelope, packaging, and...for viral vector production are separated into three different plasmids containing the transfer, envelope...

...2015). WGA-Alexa Transsynaptic Labeling in the Phrenic Motor System of Adult Rats: Intrapleural Injection...

...V A, C, or G W A or T Y C or T Amino Acids Name Three Letter Code Single Letter Code Codons (RNA) Alanine... CCG Serine Ser S UCU, UCC, UCA, UCG, AGU,AGC Threonine Thr T ACU, ACC, ACA, ACG Tryptophan Trp W UGG ...GUG Start AUG* Stop UAG (amber), UGA (opal), UAA (ochre) *AUG is the most common start codon. Alternative...

...insulators, and silencers. Promoter Regions There are three main portions that make up a promoter: core promoter... initiate the transcription of genes. There are three types of RNA polymerases that all transcribe different...

...Production Antibodies are typically produced in one of three different methods: in animals, in cultured immune...secondaries, are usually much easier to select. There are three main considerations. What is the source of your ...

...intracellular calcium stores. There are currently three Gq DREADDs based on human muscarinic receptors: ...

...target region, effectively turning Cas3 into a DNA “shredder” system that can cause large genomic deletions...