We narrowed to 407 results for: HRE
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TypeBlog Post...2010). As the field moves toward standardized thresholds for validating CRISPR experiments, many are turning...
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Plasmids 101: Modular Cloning Applications and Kits
TypeBlog Post...plant biologists, Addgene has not one, not two, but three CRISPR MoClo kits for plants that work for both ... -
Which Fluorescence Microscopy Technique is Best for Me?
TypeBlog Post... single, thin plane within a relatively thicker three dimensional sample (see Figure 1); similar to an... -
Fluorescent Proteins 101: Monitoring Cell Mobility Using Fluorescent Proteins
TypeBlog Post...technique was first Introduced to immunology by three papers in the journal Science in 2002. Now, this... -
Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins
TypeBlog Post...Jellyfish (Aequorea victoria) GFP (wtGFP) by mutating Threonine 203 to Histidine (T203H) (1). wtGFP normally contains... -
Cancer and the Immune System: Deciphering the Relationship
TypeBlog Post...progression. Dunn et al in 2002, introduced the “Three Es of Immunoediting” nomenclature. 1. Eliminate ... -
Antibodies 101: Flow Cytometry
TypeBlog Post...of the instrumentation A flow cytometer combines three systems to analyze single cells from a mixture (... -
Antibodies 101: Introduction to Antibodies
TypeBlog Post...antibody Fab’ fragments: An overview and comparison of three of the most versatile biosensor biorecognition elements... -
A Primer on Optogenetics: Introduction and Opsin Delivery
TypeBlog Post...Behavioral Optogenetics can be broken down broadly into three main steps: 1) Selection and expression of your... -
Selecting Your Plasmid Purification Kit
TypeBlog Post...amounts of high quality DNA. Figure 1 illustrates the three grades of DNA you may need based on their relative... -
How to Deposit Your Plasmids with Addgene
TypeBlog Post...for deposits submitted after July 1, 2025) after three years on hold, Addgene staff may release the hold... -
Starter Guide to induced Pluripotent Stem Cells (iPSCs) Part 2: Reprogramming and Transdifferentiation
TypeBlog Post...Dedifferentiation, transdifferentiation and reprogramming: three routes to regeneration. Nat Rev Mol Cell Biol, 2011... -
Antibody Validation for Flow Cytometry
TypeBlog Post...Figure 1). It can therefore be helpful to try all three when looking to label an intracellular target. ... -
The CRISPR Software Matchmaker: A New Tool for Choosing the Best CRISPR Software for Your Needs
TypeBlog Post...for automated rational design. The first of only three such databases, EENdb (published 1 Jan 2013, no ... -
Optogenetics + CRISPR, Using Light to Control Genome Editing
TypeBlog Post...-targeting gRNA and a dCas9 fusion protein with three red fluorescent tags in U2OS cells. Upon light activation... -
CRISPR Methods for Bacteria: Genome Engineering, CRISPRa, CRISPRi, Base Editing, and More
TypeBlog Post...QCascade DNA-targeting module with the crRNA, (3) three transposase proteins, and (4) the donor DNA. By ... -
Using AAV for Neuronal Tracing
TypeBlog Post...2015). WGA-Alexa Transsynaptic Labeling in the Phrenic Motor System of Adult Rats: Intrapleural Injection... -
Lentiviral Vector Guide
TypeGuide...In order to produce lentiviral vectors, you need three (or four, for third generation) plasmids: Transfer...VSV-G Safety Safe; replication incompetent by using three separate plasmids encoding the necessary viral genes...replication incompetent by using four plasmids instead of three and eliminates the requirement for Tat. Always includes...lentiviral production. All lentiviral plasmids (three or four) are transfected into HEK293T cells using... -
Adeno-associated virus (AAV) Guide
TypeGuide...replication and viral packaging. The Cap region encodes three structural capsid proteins or viral proteins (VP1...different plasmid. In order to produce rAAV vectors, three plasmids are needed: Transfer plasmid (also known...’s Molecular Biology Reference . Production The three production plasmids (transfer, packaging, and helper...Rep52, and Rep40. Cap in trans The cap gene encodes three AAV viral capsid proteins VP1, VP2, and VP3, which... -
Gamma-Retroviral Vector Guide
TypeGuide...order to produce gamma-retroviral vectors, you need three plasmids (Figure 2): Transfer plasmid — contains..., similar to lentiviral packaging methods . The three plasmids described above (envelope, packaging, and...for viral vector production are separated into three different plasmids containing the transfer, envelope...