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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 469 results for: LARGE

Showing: 381 - 400 of 469 results

  1. Plan Your Experiment

    Type
    Guide
    ...edits, including point mutations, base changes, and large insertions or deletions. For more information on...efficiencies. The template sequences can also have a large impact on editing efficiency. So while testing multiple...of AAV vectors is that the packaging limit (how large of a DNA sequence you can actually package into ...edit. Even if you provided a homology template, a large percentage of DNA breaks will be repaired by NHEJ... Can't distinguish specific edits PCR Knockouts Large insertions/deletions Some prime and base edits Can...inefficient methods and often require screening a larger pool of cells to find one with your desired edit...

  2. Adeno-associated virus (AAV) Guide

    Type
    Guide
    ...activates the necessary cellular machinery to produce large amounts of rAAV vectors. The E1 gene is not included...distributing widely throughout the organism to target large or diffused biological structures. Specifically,...visualize connections between neurons within the brain. Large-scale examples of mapping the brain through neuronal... capacity Another strategy to deliver cargo too large to fit within the rAAV capsid, such as Cas9-derived...which has shown higher efficiency in delivering large genes makes use of split inteins, proteins that ...be expressed. This method allows for delivery of larger transgenes, but expression is still much less efficient...

  3. Lentiviral Vector Guide

    Type
    Guide
    ...First-generation First-generation lentiviral plasmids are largely defunct due to their lack of safety improvements...on different plasmids, the viral genome remains largely intact. First-generation plasmids include (Figure...full-length virus. The potential for oncogenesis is largely based on whether or not the specific insert contained...identifies alterations in chromatin architecture through large-scale mapping of lentiviral integration sites. Nature...

  4. Adenovirus Guide

    Type
    Guide
    ...vectors, rAdV vectors can provide a large transgene packaging capacity. Large protein-coding genes and complex... and third-generation vectors. This capacity is large enough to carry the Cas9 gene and a gRNA expression...shorter period of time or when needing to package larger cargo. CRISPR-Cas9 Delivery As compared to other...backbone plasmid that lacks E1, E3, and E4. Useful for larger inserts. pAdTrack Class of shuttle/transfer vectors...

  5. Gamma-Retroviral Vector Guide

    Type
    Guide
    ... protocols for fast and efficient generation of large-scale, high-titer preps. This is thanks to established...full-length virus. The potential for oncogenesis is largely based on whether or not the specific insert contained...

  6. Antibody Guide

    Type
    Guide
    ...above are used to create hybridomas, which produce large amounts of a single antibody with little variation...antibody is made up of four protein chains: two large heavy chains and two small light chains. These four... fluorophore colors in flow cytometry, but the largest panels used currently are in the mid-forties. When...

  7. Modular Cloning Guide

    Type
    Guide
    ...Christopher A. O'Callaghan Toolkit for assembling large DNA fragments based on methylation-switching. 4G-Cloning...genetic engineering of S. cerevisiae , enabling larger multi-gene constructs, multiplexed CRISPR editing...

  8. Quantifying DNA? Here are Five DNA Quantification Methods to Consider

    Type
    Blog Post
    ...electrophoresis. Smaller fragments migrate quicker than larger fragments. These fragments are quantified over ...that smaller fragments are quantified first before larger fragments. Figure 1: The quantification of...time. Smaller fragments migrate more quickly than larger fragments. Image from OpenWetWare. This method...

  9. Multiplex Genome Editing with CRISPR-Cpf1

    Type
    Blog Post
    ...with a transfectable cell line. 2) They require larger expression vectors which are often more difficult...into viral vectors. Cas9 multiplexing vectors are larger because they require regulatory sequences to allow...individual promoters).  spCas9 and its gRNAs are also larger than their Cpf1 counterparts. Table 2. Cas9 Multiplexing...

  10. Addgene Depositors Get More Citations

    Type
    Blog Post
    ...outlier, this story had us wondering: is there a larger trend here? Do papers associated with Addgene deposits...deposits are more highly cited, we went to one of the largest bibliographic databases available, Web of Science...

  12. Addgene’s Viral Service - Why Virus? Why Now?

    Type
    Blog Post
    ...preps. This new Viral Service represents Addgene’s largest new initiative since we started distributing plasmids...support we are able to provide faster access to a larger number of viral preps. Visit our Sponsors and Supporters...

  13. CRISPR 101: Making a Knock-In Cell Line

    Type
    Blog Post
    ...hundred bps of sequence. However, if you have a larger knock-in (200 bp – 2 kb), a dsDNA donor will need...oligonucleotides. These donors traditionally have larger homology arms in the 500 bp to 1 kb range, however...

  14. Transferable Skills Guide: Time Management

    Type
    Blog Post
    ...accomplishment while the longer tasks will give you larger payoffs later. Don’t be afraid to accomplish some...ve also recently started using Trello to manage larger, multi-person projects. Day-to-day, flexible to-do...

  15. Optogenetics Guide

    Type
    Guide
    ...function by reversibly sequestering targets into large, optically assembled protein architectures in living...(CrChR2) 470 ChR2/H134 Widely used variant with larger photocurrent compared to CrChR2 450 ChETA E123T...

  16. Centrifugation

    Type
    Protocol
    ...microfuge tubes used in labs, though some larger models can hold larger conical tubes. Depending on the centrifuge...speeds to their smaller cousins, but can hold much larger containers. Ultracentrifuges are also generally...generally floor sized centrifuges that can hold larger containers, but they can also rotate at much higher speeds...

  17. Using a Light Microscope Protocol

    Type
    Protocol
    ...are magnification (the ability to make an image larger) and resolution (the ability to distinguish between...diaphragm and use the coarse focus adjustment knob (the larger of the two focus knobs) until you can see the edges...magnification, you will notice that the objectives get larger. Be careful to avoid hitting your sample with the...magnification, remember that the objectives are larger. Adjust your focus slowly so that you don't accidentally...
Showing: 381 - 400 of 469 results