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We narrowed to 464 results for: LARGE

Showing: 381 - 400 of 464 results

  1. Gamma-Retroviral Vector Guide

    Type
    Guide
    ... protocols for fast and efficient generation of large-scale, high-titer preps. This is thanks to established...full-length virus. The potential for oncogenesis is largely based on whether or not the specific insert contained...

  2. Antibody Guide

    Type
    Guide
    ...above are used to create hybridomas, which produce large amounts of a single antibody with little variation...antibody is made up of four protein chains: two large heavy chains and two small light chains. These four... fluorophore colors in flow cytometry, but the largest panels used currently are in the mid-forties. When...

  3. Quantifying DNA? Here are Five DNA Quantification Methods to Consider

    Type
    Blog Post
    ...electrophoresis. Smaller fragments migrate quicker than larger fragments. These fragments are quantified over ...that smaller fragments are quantified first before larger fragments. Figure 1: The quantification of...time. Smaller fragments migrate more quickly than larger fragments. Image from OpenWetWare. This method...

  4. Multiplex Genome Editing with CRISPR-Cpf1

    Type
    Blog Post
    ...with a transfectable cell line. 2) They require larger expression vectors which are often more difficult...into viral vectors. Cas9 multiplexing vectors are larger because they require regulatory sequences to allow...individual promoters).  spCas9 and its gRNAs are also larger than their Cpf1 counterparts. Table 2. Cas9 Multiplexing...

  5. Optogenetics Guide

    Type
    Guide
    ...function by reversibly sequestering targets into large, optically assembled protein architectures in living...(CrChR2) 470 ChR2/H134 Widely used variant with larger photocurrent compared to CrChR2 450 ChETA E123T...

  6. Addgene Depositors Get More Citations

    Type
    Blog Post
    ...outlier, this story had us wondering: is there a larger trend here? Do papers associated with Addgene deposits...deposits are more highly cited, we went to one of the largest bibliographic databases available, Web of Science...

  8. Addgene’s Viral Service - Why Virus? Why Now?

    Type
    Blog Post
    ...preps. This new Viral Service represents Addgene’s largest new initiative since we started distributing plasmids...support we are able to provide faster access to a larger number of viral preps. Visit our Sponsors and Supporters...

  9. CRISPR 101: Making a Knock-In Cell Line

    Type
    Blog Post
    ...hundred bps of sequence. However, if you have a larger knock-in (200 bp – 2 kb), a dsDNA donor will need...oligonucleotides. These donors traditionally have larger homology arms in the 500 bp to 1 kb range, however...

  10. Transferable Skills Guide: Time Management

    Type
    Blog Post
    ...accomplishment while the longer tasks will give you larger payoffs later. Don’t be afraid to accomplish some...ve also recently started using Trello to manage larger, multi-person projects. Day-to-day, flexible to-do...

  11. Centrifugation

    Type
    Protocol
    ...microfuge tubes used in labs, though some larger models can hold larger conical tubes. Depending on the centrifuge...speeds to their smaller cousins, but can hold much larger containers. Ultracentrifuges are also generally...generally floor sized centrifuges that can hold larger containers, but they can also rotate at much higher speeds...

  12. Using a Light Microscope Protocol

    Type
    Protocol
    ...are magnification (the ability to make an image larger) and resolution (the ability to distinguish between...diaphragm and use the coarse focus adjustment knob (the larger of the two focus knobs) until you can see the edges...magnification, you will notice that the objectives get larger. Be careful to avoid hitting your sample with the...magnification, remember that the objectives are larger. Adjust your focus slowly so that you don't accidentally...

  14. Molecular Biology Reference

    Type
    Guide
    ...(mammalian, yeast, bacterial, etc.), depending largely on which promoter is used to initiate transcription...

  20. Science as Art

    Type
    Blog Post
    ...creations can go on display for all to enjoy. The largest competition of this kind is Princeton University...
Showing: 381 - 400 of 464 results