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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 407 results for: pho

Showing: 381 - 400 of 407 results

  1. Pipetting Protocol

    Type
    Protocol
    ...Information Anatomy of a Pipette This front view photo of a P1000 pipette shows the plunger at the top,... pipette tip off of the pipette. This side view photo of a P1000 pipette shows the volume adjustment ring...ready to begin using the pipette. To the right is a photo of how you might hold a pipette. Without moving ...

  2. Antibody Validation Using the Indirect ELISA Method

    Type
    Protocol
    ...of each well at 450 nm on the spectrophotometer. Use the spectrophotometer to generate a standard curve...an automated ELISA plate washer. Equipment Spectrophotometer compatible with 96-well plates 1–10 µL single...

  3. Ligation Independent Cloning

    Type
    Protocol
    ...cloning scars"). A “stuffer” sequence allows for electrophoretic separation of linearized vector from the reaction...linearized vector from the reaction mixture by gel electrophoresis followed by gel purification . The cut vector...

  5. What is Polymerase Chain Reaction (PCR)

    Type
    Protocol
    ...product can be verified based on size by gel electrophoresis . Protocol Video Watch the protocol video below...rate of the DNA polymerase. Deoxynucleoside Triphosphates (dNTPs): Are the building blocks added one at...

  6. Kit Free RNA Extraction

    Type
    Protocol
    ...the quality of your RNA sample(s) using a spectrophotometer (such as a Nanodrop), agarose gel, or bioanalyzer...

  8. Protocol - How to Design Primers

    Type
    Protocol
    ...Reaction Plasmid Cloning by PCR Agarose Gel Electrophoresis Primer Design for PCR Oligonucleotide primers...

  9. Weighing Reagents Protocol

    Type
    Protocol
    ...will be weighed out Procedure The balance in the photo above has a capacity of 200 g, as indicated by the...

  10. Centrifugation

    Type
    Protocol
    ... will have essentially the same components. The photo to the left shows a front view of an open tabletop...

  13. Western Blot

    Type
    Protocol
    ...Pipette controller Pipette tips and pipettes Spectrophotometer Heat block Mini gel tank chamber Power supply...

  15. Optogenetics Guide

    Type
    Guide
    ...)/~500 (off) PhoCl Photocleavable protein engineered from a green-to-red photoconvertible fluorescent ...enhancements include: Increased photocurrent amplitude Examples: iChloC, SwiChRca, Phobos, Aurora Browse Channelrhodospin...reduces photocurrent amplitude 490 ChR/T159C T159C mutation; displays increased photocurrents 470 SFO/...bacterial photoreceptors to create protein systems controlled by light. These “photoswitchable” proteins...Kv) channels fused to LOV2 photoswitch. Illumination causes the photoswitch to unfold, lowering the toxin's...synthetic, photosensitive kinase, which uses the ubiquitous flavin mononucleotide as its chromophore, and phosphorylates... 400 UVR8 domains UVR8 plant photoreceptor protein that forms photolabile homodimers with slow reversal...

  16. Lentivirus ddPCR Titration

    Type
    Protocol
    ...Determine the concentration of each sample on a spectrophotometer. Prepare 25 ng/µL stocks of each sample. Samples...

  17. Antibody Guide

    Type
    Guide
    ... curve with known amounts of protein. Fluorophores Fluorophores are light-sensitive molecules which emit...IF uses fluorophore color to differentiate between the targets of interest, if your fluorophore is bound...the fluorophores used is important. It is best to inversely pair the brightness of the fluorophore with...If a bright fluorophore is on an abundant target, it obscures fainter signals. Fluorophores can emit signals...storing your antibody: Antibodies conjugated to fluorophores should never be stored below 4 °C. Certain isotype...molecules are horseradish peroxidase (HRP) and fluorophores. Horseradish peroxidase (HRP) HRP performs a...specific wavelength. While more expensive than HRP, fluorophores are available in a range of colors activated...

  18. Gamma-Retroviral Vector Guide

    Type
    Guide
    ...envelope, Phoenix-ECO (for infection of mouse and rat cells), or an amphotropic envelope, Phoenix-AMPHO (for...of the viral packaging cell line. For example, Phoenix™ is a popular second-generation gamma-retroviral... produced in just a few days. Another variant, Phoenix-gp, contains only gag-pol and enables additional...

  19. Chemogenetics Guide

    Type
    Guide
    ...activate neuronal firing through stimulating phospholipase C, which releases intracellular calcium stores...molecule driven by FRET-modified bioluminescence. Neurophotonics. 11:021005. PMID: 38450294 (Link opens in a...luminopsins with improved coupling efficiencies. Neurophotonics. 11:024208. PMID: 38559366 (Link opens in a...

  20. CRISPR Guide

    Type
    Guide
    ...SpCas9-HF1 - disrupt Cas9’s interactions with DNA phosphate backbone HypaCas9 - increase Cas9 proofreading...fusing the anti-CRISPR protein AcrIIA4 with the photosensitive LOV2 domain . Several labs have also controlled...: Epigenetics Visualizing Genomic Loci Using Fluorophores CRISPR imaging has numerous advantages, including...11: Overview of visualizing genomic loci with fluorophores using CRISPR Browse Plasmids: Visualize Purify...Yoshimi, K., Okuzaki, Y., Gee, P., Kunihiro, Y., Sonpho, E., Xu, H., Sasakawa, N., Naito, Y., Nakada, S...
Showing: 381 - 400 of 407 results