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We narrowed to 677 results for: RON

Showing: 201 - 210 of 677 results

  1. Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

    Type
    Blog Post
    ...conserved in mammals, expressed in neurons, and are especially abundant in neuronal synapses. shRNA silencing ...way to guide inheritance that might be less error prone than DNA replication. He too mentioned how “surprising...regulate at the DNA level and 1 at the RNA level. Udi Qimron from Tel Aviv University then spoke about using...treatment resulting in major problems in hospitals. Dr. Qimron proposes using bacterial phage vectors with CRISPR...

  2. PhD Applications After COVID

    Type
    Blog Post
    ...interview is another way to make the virtual environment more comfortable and help you to keep yourself...institution, you can get a better sense of the environment of the department, the lab setting, and how students... can also be nerve wracking to ask questions in front of other prospective students; an online meeting...by talking to current students about the work environment and social aspects of the department, you may...Options Thanks to the much more developed virtual environment resulting from the pandemic, students who are...

  3. Academic vs. Industry Postdocs

    Type
    Blog Post
    ... having already been immersed in an industry environment, have a better understanding of the fast, deadline-driven...up in an industry job, being immersed in that environment sooner rather than later can be helpful. Publishing...project might be challenging. In an academic environment, surrounded by lab members who are all working...Novartis, Biogen, Amgen, New England Biolabs, Regeneron, and Genentech, among others, and the list is ...opportunity to do some great science in a supportive environment. Both paths will give you myriad opportunities...

  4. 3D Printing Meets CRISPR Cas9

    Type
    Blog Post
    ...images. In the early days of structural biology, Byron Rubin, a crystallographer working with Jane Richardson...solution to this problem with the development of Byron’s Bender – a mechanical device that could be used...protein’s structure into experimentally determined electron density, have been known to say something silly... in the education community that All models are wrong….Some models are useful. While a physical model ...first Cas9 model is certainly useful, it is also wrong in at least one serious way. We represented the ...

  5. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    ... In LSFM the laser light sheet, typically 2-6  microns, illuminates only one thin plane of the sample ...image acquisition is performed using cameras with electron multiplying couple-charged device (EMCCD) or complementary...of transgenic mice in which a subpopulation of neurons are brightly labeled with GFP (e.g. Thy1-YFP-H ...LSFM with multiple tissue clearing strategies and neuronal labeling methods will greatly aid our understanding...331–336. Pubmed. P. Keller, M.B. Ahrens.  (2015) Neuron, 85, 462-483. Pubmed. E.H.K. Stelzer. (2015). Nature...

  6. Delivery Methods for Generating iPSCs

    Type
    Blog Post
    ...cloning capacity of ~8 kb, so either a single polycistronic vector or multiple single-gene vectors can be...endogenous pluripotency genes. Retroviral vectors are prone to incomplete silencing of reprogramming transgenes...differentiation potential. Finally, retroviruses are prone to insertional mutagenesis and random integration...retroviral vectors. This makes it possible to use a polycistronic cassette that expresses multiple reprogramming...factors can be delivered either as a single polycistronic transgene or with four different adenoviruses... ~14.5kb minicircle expressing a 2A peptide polycistronic cassette comprised of OCT4, SOX2, LIN28, NANOG...

  7. Optogenetics + CRISPR, Using Light to Control Genome Editing

    Type
    Blog Post
    ...dimeric green fluorescent protein (pdDronpa) (Zhou et al., 2018). pdDronpa dimers dissociate in response to... to cyan light (500nm). By fusing the two pdDronpa domains to the sites flanking the DNA-binding cleft...binding in the dark. Upon light stimulation, the pdDronpa moieties move apart, allowing DNA binding and ...Cas9, dCas9 Gene editing Zhou et al., 2018   pdDronpa Cas9, dCas9 Gene editing, transcription Light-Dependent...photoactivatable transcription systems to induce neuronal differentiation. Nat Methods 14:963–966 . https...

  8. Better Dyeing Through Chemistry & Small Molecule Fluorophores

    Type
    Blog Post
    ...dependent on the photon budget and pushing the frontiers of fluorescence microscopy often requires more...easier and more functional in complex biological environments such as live cells and tissues (Figure 1). These...several others can be made fluorogenic using environmentally sensitive fluorophores. Overall, self-labeling...photon budget and allow us to push further the frontiers of biological imaging.  *Worried about your budget...Central PMCID: PMC4344395. 3. Lavis, Luke D., and Ronald T. Raines. "Bright ideas for chemical biology."...

  9. Lentiviral Vector Uses and Overview

    Type
    Blog Post
    ...system that could do both, Addgene depositor Didier Trono and collaborators turned to the lentivirus HIV-1...experimental use. In 1996, Naldini et al. transduced rat neurons in vivo with beta-gal and other reporters, and ...vectors are incredibly popular in the lab - the Trono lab’s lentiviral packaging and envelope plasmids...Gallay P, Ory D, Mulligan R, Gage FH, Verma IM, Trono D. Science. 1996 Apr 12;272(5259):263-7. PubMed ...Dull T, Zufferey R, Kelly M, Mandel RJ, Nguyen M, Trono D, Naldini L. J Virol. 1998 Nov;72(11):8463-71. ...

  10. Supporting Reproducibility with a Connected ELN

    Type
    Blog Post
    ...virtue of liberating data from paper into an electronic environment and hence making it searchable and shareable...founder of Research Space. There are many types of electronic lab notebooks (ELNs), each with its own pros ... of Research Space, which provides the RSpace electronic lab notebook. Rory is based in Cambridge, Massachusetts...on the Addgene blog Find tips on choosing an electronic lab notebook Watch a livestream of the Minisymposium...
Showing: 201 - 210 of 677 results