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Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 423 results for: NUC

Showing: 401 - 420 of 423 results

  2. General Transfection

    Type
    Protocol
    ... the plasmid should also be propagated in an endonuclease negative E. coli strain such as NEB stable. ...

  4. Immunocytochemistry

    Type
    Protocol
    ... on a rocking platform. (Optional) Counterstain nuclei with 500 µL of 300 nM DAPI working solution for...

  5. Lentivirus Production

    Type
    Protocol
    ...the plasmids should also be propagated in an endonuclease negative E. coli strain such as NEB stable. ...

  7. Western Blot

    Type
    Protocol
    ... hard to extract proteins such as those in the nucleus. Incubate on ice for 30 min . Centrifuge lysate...

  8. AAV ddPCR Titration

    Type
    Protocol
    ...Reverse ITR Primer (10 µM) 1.8 µL 16.2 µL 900 nM Nuclease-free water 5.9 µL 71.55 µL Total Volume 20 µL ...

  9. AAV Production in HEK293 Cells

    Type
    Protocol
    ... ~18 mL from steps 12 & 13). Benzonase is an endonuclease that will degrade any residual DNA carried over...

  10. Trimmer Lab NeuroMab Collection

    Type
    Collection
    ...transfection of suspension-growing human 293-EBNA1 cells. Nucleic Acids Res . 30:e9. PMID: 11788735 (Link opens in...

  11. CRISPR Guide

    Type
    Guide
    ...like zinc finger nucleases (ZFNs) or transcription-activator-like effector nucleases (TALENs) required...popular Cas endonuclease, with many modified versions engineered to increase specificity and nuclease activity...increase nuclease fidelity SpCas9-NG - NG; increase in vitro activity SpG - NGN; increase nuclease activity...break; the wild type function of most Cas nucleases dCas9 Nuclease dead Cas9, an enzymatically inactive form...required scientists to design and generate a new nuclease pair for every genomic target. Due to its comparative...RNA ( gRNA or sgRNA ) and a CRISPR-associated endonuclease (Cas enzyme). The gRNA is a short synthetic ...necessary for Cas-binding and a user-defined ∼20-nucleotide spacer that defines the genomic target to be ...

  12. Molecular Biology Reference

    Type
    Guide
    ... transcribed from DNA. DNA is comprised of 4 nucleotides or bases, adenine, thymine, cytosine, and guanine... double stranded helix. The order of these 4 nucleotides makes up the genetic code and provides the instructions...amino acids. Each amino acid is encoded for by 3 nucleotides termed a codon. As there are only 20 natural ...CRISPR technology. CRISPR is composed of a DNA endonuclease and guide RNAs that target specific locations...sequence DNA” to identify the order of these four nucleotide bases in a particular DNA strand. Sequencing ...migrates down the single stranded DNA adding nucleotides according to the sequence of the complementary...interest. In vitro DNA replication requires the 4 nucleotides, a DNA polymerase enzyme, the template DNA to...

  13. Cloning

    Type
    Guide
    ...molecular biology enzymes: 5' exonuclease, polymerase and ligase. 5' exonuclease digests the 5' end of dsDNA...properties of type IIS restriction endonucleases. These endonucleases cut dsDNA at a specified distance...) relies on the 3'-5' exonuclease activity of T4 DNA polymerase. An exonuclease is an enzyme which removes...removes nucleotides from the end of a DNA strand. In LIC, the T4 DNA polymerase’s exonuclease activity ...Restriction Enzyme Cloning Restriction enzyme (endonuclease) based molecular cloning is the "classic" cloning...polymerases synthesize DNA molecules using the 4 nucleotides and lastly DNA ligase fuses DNA strands together...together in one isothermal reaction. First the 5’ exonuclease chews back the 5’ ends of your DNA fragments ...

  14. Optogenetics Guide

    Type
    Guide
    ...genetically-encoded nuclear Cry2 fusion can thus direct a CIB1-protein fusion to the nucleus upon light activiation...voltage (LOV) domain of FKF1 LINUS light-inducible nuclear localization signal based on the LOV2 domain of...photosensitive kinase, which uses the ubiquitous flavin mononucleotide as its chromophore, and phosphorylates the ...

  15. Gamma-Retroviral Vector Guide

    Type
    Guide
    ... increased nuclear export. Psi (Ѱ) in cis RNA packaging signal; recognized by nucleocapsid proteins and...driven by strong enhancers and active promoters. Nucleic Acids Research , 42 (7), 4257–4269. https://doi.org...retroviral particle containing matrix, capsid, and nucleocapsid components. pol in trans Precursor protein containing...

  16. Lentiviral Vector Guide

    Type
    Guide
    ...stimulates the expression of transgenes via increased nuclear export. RRE in cis Rev Response Element; he sequence...) in cis RNA packaging signal; recognized by nucleocapsid proteins and essential for efficient viral packaging...lentiviral particle containing matrix, capsid, and nucleocapsid components. pol in trans Precursor protein containing...and partially spliced transcripts to facilitate nuclear export. Provided by a separate plasmid from gag...

  17. Antibody Guide

    Type
    Guide
    ...interactions between DNA and protein. Micrococcal nuclease digestion is used to shear the DNA in these assays...specific to your assay. Most IF techniques use a nuclear stain, such as DAPI, to identify individual cells...cells. Immunofluorescence experiments often use a nuclei marker to identify individual cells and additional... PE, and APC 4-color microscopy panel: DAPI for nuclei, AF488, AF555, and AF647 Antibody Selection Selecting...

  18. Promoters

    Type
    Guide
    ...found in eukaryotic cells that package DNA into nucleosomes. Histone binding prevents the initiation of transcription...Pribnow box which usually consists of the six nucleotides, TATAAT. Proximal Promoter Further upstream from...response element promoter U6 Constitutive Human U6 nuclear promoter for small RNA expression UAS Specific ...

  19. Modular Cloning Guide

    Type
    Guide
    ...expressing multiple gRNAs (up to seven) with a Cas9 nuclease, nickase, dCas9, or dCas9-FokI fusion for use ...Vladimir Nekrasov 95 plasmids including CRISPR/Cas nucleases, base editors, gRNA backbones, and promoters for..., TALEN Feng Zhang Plasmids for building TALE nucleases (TALENs) and transcription factors (TALE-TFs) ...

  20. Plan Your Experiment

    Type
    Guide
    ...increase or decrease depending upon the specific nucleotides within the selected target sequence. Additionally...the Cas enzyme and gRNA, or as a functional ribonucleoprotein (RNP). Created with BioRender.com. When choosing...delivery Lastly, you can deliver Cas-gRNA ribonucleoprotein (RNP) complexes directly into your target ...
Showing: 401 - 420 of 423 results