We narrowed to 417 results for: NUC

... hard to extract proteins such as those in the nucleus. Incubate on ice for 30 min . Centrifuge lysate...

...Reverse ITR Primer (10 µM) 1.8 µL 16.2 µL 900 nM Nuclease-free water 5.9 µL 71.55 µL Total Volume 20 µL ...

... ~18 mL from steps 12 & 13). Benzonase is an endonuclease that will degrade any residual DNA carried over...

...transfection of suspension-growing human 293-EBNA1 cells. Nucleic Acids Res . 30:e9. PMID: 11788735 (Link opens in...

...like zinc finger nucleases (ZFNs) or transcription-activator-like effector nucleases (TALENs) required...popular Cas endonuclease, with many modified versions engineered to increase specificity and nuclease activity...increased nuclease fidelity SpCas9-NG — NG; increased in vitro activity SpG — NGN; increased nuclease activity...break; the wild type function of most Cas nucleases dCas9 Nuclease dead Cas9, an enzymatically inactive form...required scientists to design and generate a new nuclease pair for every genomic target. Due to its comparative...RNA ( gRNA or sgRNA ) and a CRISPR-associated endonuclease (Cas enzyme). The gRNA is a short synthetic ...necessary for Cas-binding and a user-defined ∼20-nucleotide spacer that defines the genomic target to be ...

...and RNA into proteins. DNA is comprised of four nucleotide bases: adenine, thymine, cytosine, and guanine...double-stranded helix. To create the double helix, the nucleotides on the opposing strands of DNA form hydrogen ...with T, and C always pairs with G. The order of nucleotides makes up the genetic code and provides the instructions...process called transcription. RNA uses the same nucleotide bases as DNA, except thymine is replaced by uracil...acids. Each amino acid is encoded for by three nucleotides, termed a codon. There are 64 codon combinations...CRISPR technology. CRISPR is composed of a DNA endonuclease and guide RNAs that target specific locations...Scientists often sequence DNA to identify the order of nucleotide bases in a particular DNA strand. Sequencing ...

...properties of type IIS restriction endonucleases. These endonucleases cut dsDNA at a specified distance...' to 5' exonuclease activity of T4 DNA polymerase. In LIC, the T4 DNA polymerase’s exonuclease activity...Restriction Enzyme Cloning Restriction enzyme (endonuclease) molecular cloning is the "classic" cloning ...of three common molecular biology enzymes: 5' exonuclease, polymerase, and ligase. In Gibson assembly, ...together in one isothermal reaction. First, the 5’ exonuclease chews back the 5’ ends of your DNA fragments,...polymerase then closes the gap created by the 5’ exonuclease. Finally, ligase seals the nicks in the DNA to...potential to be completely degraded by the 5' exonuclease. Read more in our Gibson Assembly blog post ....

... increased nuclear export. Psi (Ѱ) in cis RNA packaging signal; recognized by nucleocapsid proteins and...driven by strong enhancers and active promoters. Nucleic Acids Research , 42 (7), 4257–4269. https://doi.org...retroviral particle containing matrix, capsid, and nucleocapsid components. pol in trans Precursor protein containing...

...genetically-encoded nuclear Cry2 fusion can thus direct a CIB1-protein fusion to the nucleus upon light activiation...voltage (LOV) domain of FKF1 LINUS light-inducible nuclear localization signal based on the LOV2 domain of...photosensitive kinase, which uses the ubiquitous flavin mononucleotide as its chromophore, and phosphorylates the ...

...stimulates the expression of transgenes via increased nuclear export. RRE in cis Rev Response Element; the sequence...) in cis RNA packaging signal; recognized by nucleocapsid proteins and essential for efficient viral packaging...lentiviral particle containing matrix, capsid, and nucleocapsid components. pol in trans Precursor protein containing...and partially spliced transcripts to facilitate nuclear export. Provided by a separate plasmid from gag...

...interactions between DNA and protein. Micrococcal nuclease digestion is used to shear the DNA in these assays...specific to your assay. Most IF techniques use a nuclear stain, such as DAPI, to identify individual cells...cells. Immunofluorescence experiments often use a nuclei marker to identify individual cells and additional... PE, and APC 4-color microscopy panel: DAPI for nuclei, AF488, AF555, and AF647 Antibody Selection Selecting...

...expressing multiple gRNAs (up to seven) with a Cas9 nuclease, nickase, dCas9, or dCas9-FokI fusion for use ...Vladimir Nekrasov 95 plasmids including CRISPR/Cas nucleases, base editors, gRNA backbones, and promoters for..., TALEN Feng Zhang Plasmids for building TALE nucleases (TALENs) and transcription factors (TALE-TFs) ...

...increase or decrease depending upon the specific nucleotides within the selected target sequence. Additionally...the Cas enzyme and gRNA, or as a functional ribonucleoprotein (RNP). Created with BioRender.com. When choosing...delivery Lastly, you can deliver Cas-gRNA ribonucleoprotein (RNP) complexes directly into your target ...

...wild-type AAVs enter a host cell, they uncoat in the nucleus and present as linear DNA molecules. In the absence...stimulates the expression of transgenes via increased nuclear export. Poly(A) signal in cis The polyadenylation...polyadenylation or poly(A) signal aids in the nuclear export of RNA and RNA translation, and promotes RNA transcript...

...and histones (proteins that package DNA into nucleosomes) also bind the TATA box. Histone binding prevents...phospholycerate kinase gene U6 Constitutive Mammalian U6 nuclear promoter for small RNA expression (species-specific...

...septicum alpha-toxin , and resistance to to the nucleotide analog 6-thioguanine . Next-generation sequencing...

...remain as extrachromosomal elements within the nucleus, normally as double-stranded circular episomes....