We narrowed to 497 results for: RAP

1. Academic vs. Industry Postdocs

   Type

   Blog Post

   ...drawn to the idea of working as a team to develop therapies that could directly impact patients. And, to be...

2. Tips for a 1st time AAV user (by a Rookie AAV user)

   Type

   Blog Post

   ...inverted terminal repeat sequences. Human gene therapy methods, 23 1, 18-28. PubMed PMID: 22428977. 2....

3. Viral Vectors 101: Voltage Indicators

   Type

   Blog Post

   ... this method have been to tag, cage, uncage, and trap the chemical fluor with the genetically encoded ...

4. Antibodies 101: Conventional vs Spectral Flow Cytometry

   Type

   Blog Post

   ...tools available to immunologists, allowing for the rapid analysis of cell populations within a heterogenous...

5. When is a Monomer not a Monomer? The Top Three Ways Your Favorite Fluorescent Protein Oligomerizes in Cells

   Type

   Blog Post

   ...contacts that bring apposing membranes together and can wrap the membranes into stacked structures including ...

6. Genome-wide Screening Using CRISPR

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   Blog Post

   ... resistance to drugs and can identify future therapeutic targets for diseases causing uncontrolled cell...

7. Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards

   Type

   Blog Post

   ...confinement involves performing experiments in a geographic area where escaped organisms won't be able to...

8. Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection

   Type

   Blog Post

   ...anticipate plugging into the probiotic and cellular therapeutic space. If you were to look forward to the future...

9. Chemogenetics Guide

   Type

   Guide

   ... 4 ). Additional varenicline analogs, termed ultrapotent PSEMs (or uPSEMs), have also been developed as..., Basu J, Michaelides M, Sternson SM (2019). Ultrapotent chemogenetics for research and potential clinical...potent and selective chemogenetic actuator enables rapid neuronal and behavioral modulations in mice and ... engineer a receptor-effector complex for gene therapy. J Biol Chem. 276:1596-6. PMID: 11402033 (Link ...

10. The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data

    Type

    Blog Post

    ...illuminate biology and enable next-generation therapeutics. Prime editing, base editing, PACE, and DNA-...

11. Plasmids 101: Secondary Nanobody Toolbox

    Type

    Blog Post

    ...site-specific labeling for super-resolution imaging, rapid epitope-mapping and native protein complex isolation...

12. Transferable Skills Guide: Identifying Your Transferable Skills

    Type

    Blog Post

    ...making connections, avoid falling into an identity trap where you assume your technical skills define you...

13. AAVs CREATed for Gene Delivery to the CNS and PNS

    Type

    Blog Post

    ...translational applications (mechanistic studies and gene therapy treatments for neurological diseases). The Gradinaru...

14. Tips for arabidopsis transformation

    Type

    Blog Post

    ... PMC3759027. 2 Harrison, S. J. et al. (2006) ‘A rapid and robust method of identifying transformed Arabidopsis...

15. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

    Type

    Blog Post

    ... using Tn5), and as knock-in tools for human therapeutics (e.g. using Sleeping Beauty). Despite their ...

16. Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

    Type

    Blog Post

    ...example), as this can also be perturbed by supraphysiological expression levels. BiFC quantification This...

17. Viral Vectors 101: The Retroviral Lifecycle

    Type

    Blog Post

    ...infection; however this would not be great for a gene therapy vector! For viral vectors, only the protein for...

18. CRISPR 101: Making a Knock-In Cell Line

    Type

    Blog Post

    ...types. Variance can be caused by cell cycle rates; rapidly diving cells will have higher HR frequencies than...

19. Uncovering Ancestral Bias in CRISPR Research

    Type

    Blog Post

    ...deprioritize genes that could be good targets for therapeutic development, particularly in cancers that are...

20. Tips for Screening with Yeast Two Hybrid Systems

    Type

    Blog Post

    ...classic two hybrid system (also called the interaction trap), genes encoding proteins of interest(POI’s) are...