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We narrowed to 677 results for: RON

Showing: 221 - 230 of 677 results

  1. Three Tips to Organize Your Lab Notebooks in the New Year

    Type
    Blog Post
    ...notebooks and binder on your desk. Arrange them in chronological order (or by project, if that works better for...can easily spot the colors and text. Tip 2: Use electronic to-do lists to access your tasks on the go Never...could easily lend one to another labmate? Though electronic lab notebooks (ELNs) have gained traction with...to borrow most frequently. Copy these into an electronic file sharing tool, such as Google Drive or Dropbox...

  2. New Norepinephrine Indicators: nLightG and nLightR

    Type
    Blog Post
    ...Detection of Norepinephrine. Neuron, 102(4), 745-761.e8. https://doi.org/10.1016/j.neuron.2019.02.037 Kagiampaki... B, Representative images of HEK293T cells and neurons expressing nLightG or nLightR before/after application...dashed lines. Scale bars, 10 μm (HEK293T), 20 μm (neurons). Figure and caption adapted from Kagiampaki et...

  3. CRISPR 101: Multiplex Expression of gRNAs

    Type
    Blog Post
    ...transcript You can also multiplex gRNAs via a polycistronic transcript. Rather than being transcribed from...option described below, plasmids for making polycistronic gRNAs are also available from the Yang lab for...Plasmids: These plasmids are similar to the Csy4 polycistronic system described above, except that they use...gRNAs are flanked by glycine tRNAs to create polycistronic glycine tRNA-gRNA (PTG) constructs. Eukaryotic... Find plasmids from this paper at Addgene. 12. Ronda, Carlotta, et al. (2016). "CRMAGE: CRISPR Optimized...

  4. Selecting Your Plasmid Purification Kit

    Type
    Blog Post
    ...waste. If you are looking to reduce your lab’s environmental impact, check out Addgene’s blog post on bringing...some less obvious improvements that reduce the environmental impact. Using non-silica-based purification ...GenElute™ kits were all specifically designed with environmental sustainability in mind (Table 2). Table 2: Sustainability... Other Minimized amount of silica ACT environmental impact factor label Non-silica based, avoids...purifying! This blog post was written by Ashley Waldron, Hannah Dotson, and Nyla Naim. Resources Additional...

  5. CRISPR 101: Cytosine Transversion Editors

    Type
    Blog Post
    ...refresher if you ever lose track of which acronym is which. Acronym Full name Notes CRISPR terms gRNA... of CBEs and ABEs. This post includes a lot of acronyms, so we’ve included a glossary at the end if you...activity, and they are sometimes processed by error-prone non-homologous end joining (NHEJ). To mitigate this...edit. NHEJ Non-Homologous End Joining An error-prone cellular process to repair DSBs. Base Editing ...

  6. Bringing Sustainable Practices to the Lab: Recycling

    Type
    Blog Post
    ...our lab’s environmental impact, but we can all do more to make research more environmentally friendly.... to address, but is often ignored in the lab environment. I recently attended the Go green! Innovative...institute! What should I do in the lab to help the environment? Well, you could: Collect the NEB and/or Millipore...

  7. An Addgene Summer Internship

    Type
    Blog Post
    ...potential as a scientist. But I expected something wrong. My capstone project was based on plasmid cloning...successful clones because the bands were all in the wrong place. Except for one. My manager speculated that...my very own research project! Coming from an environment with limited opportunities and support, puts ...puts into perspective just how much one’s environment changes them. Addgene gave me the chance to become ...

  8. Viral Vectors 101: Parts of the AAV Transfer Plasmid

    Type
    Blog Post
    ...Technologies and Methods for Targeted Neuronal Manipulation. Front Neuroanat 13: . https://doi.org/10.3389...different levels of expression. For example, CAG is a strong promoter that drives very high, ubiquitous expression...expression, while the human synapsin 1 promoter is neuronal specific (Haery et al., 2019). Most AAV transfer...

  9. Celebrate Open Data Day with Addgene’s AAV Data Hub!

    Type
    Blog Post
    ... paper in Frontiers in Neuroanatomy and summarized some considerations for precise neuronal targeting ...Considerations For Precise Neuronal Targeting Using AAV Technologies Using AAV for neuronal tracing Resources...

  10. Hot Plasmids and Viral Preps - September 2021

    Type
    Blog Post
    ...available with one sgRNA per operon or gene. The sgRNA pool can also target core operons in other pneumococcal...group optimized their initial system by using a stronger promoter and different biochemical secondary energy...AAV tools to selectively control parvalbumin interneurons from the Dimidschstein Lab More GCaMP8 ready-to-use...
Showing: 221 - 230 of 677 results