We narrowed to 1,055 results for: ins;

...which often focuses more attention on the results instead of the methods. Unreported flexibility and questionable...

...They all found the support of the group to be intrinsic to their success. Their stories made it clear ...

...oligonucleotide (ssODN) template supplied by Ma et al., but instead from the mother’s wild type allele (Figure 1). ...

...up in both science journals like Nature and in mainstream media like The Atlantic. We’ve been in documentaries...

... Eric J. Perkins...

... with very little text on the slides and notes instead of written speeches. When you’re doing all that...

...scientists can receive recognition for their efforts, inspire future research, and increase their impact. Open...

...Novartis Oncology.  Bhang did her PhD work at Johns Hopkins and came to Novartis for her post-doc in January...

...seriously. As I make my rounds speaking at research institutions and at scientific conferences, I see that few...

...organizations, libraries, youth groups, and faith institutions. The conversations often move quickly into sensitive...

...since these processes are mediated by other viral proteins), but the newly released RABV cannot infect any...

...certain cell types.  Botond Roska’s lab at the Institute of Molecular and Clinical Ophthalmology Basel ...

...The day has arrived; you’ve painstakingly cared for your packaging cell line, prepped your DNA, transfected...

... decide what they’d like to work on later. For instance, AstraZeneca posts most of its positions all at...

...Highlight your achievements with specific examples. Instead of saying “I have excellent communication skills...

...genome-wide CRISPR screen presented in Morgens et al. contains three types of guides: 1) targeting guides (blue...

...excellent posts on science career topics as well. Instead of rewriting or coming up with answers, I was able...

...two options: Film outside at a distance, or film inside with one person in a room. I wouldn’t be in there...

...and sarcasm, persistent teasing, name calling, insults, intimidation, sexual harassment Isolation – including...

...analysis plan when you design your experiment, instead of after you’ve already run it.) You don’t need...

...GFP CMV Parkinson's L. Miguel Martins 14122 pcDNA3-HtrA2-EGFP S306A HTRA2 GFP CMV Parkinson's L. Miguel...Miguel Martins 14124 pcDNA3-delta133HtrA-EGFP HTRA2 GFP CMV Parkinson's L. Miguel Martins 14126 pET20b-delta133HtrA2...Miguel Martins 15939 pcDNA3-HtrA2-FLAG S142D HTRA2 Flag CMV Parkinson's L. Miguel Martins 15940 pcDNA3-HtrA2...Flag CMV Parkinson's L. Miguel Martins 16153 pET20b-delta133HtrA2 S142A HTRA2 His T7 Parkinson's L. Miguel...Miguel Martins 16154 pET20b-delta133HtrA2 S142D HTRA2 His T7 Parkinson's L. Miguel Martins 16155 pET20b-...His T7 Parkinson's L. Miguel Martins 16156 pET20b-delta133HtrA2 S400D HTRA2 His T7 Parkinson's L. Miguel...Miguel Martins 16157 pET20b-delta133HtrA2 A141S HTRA2 His T7 Parkinson's L. Miguel Martins 16158 pET20b-...

...inactive “dead” Cas9 (dCas9) contains mutations in both nuclease domains, D10A and H840A, resulting in...naturally-occurring Acr (Anti-CRISPR) proteins can help control CRISPR activity. Acr proteins have evolved in phages ...bidirectional insertions — meaning they may not be suitable for applications where orientation of the insert is ...exhibit almost unidirectional insertions but are more prone to off-target insertions. And in the case of both... edits. These proteins, like Cre recombinase or phage derived serine integrases, insert donor DNA at “...used to install the LSR Bxb1’s landing site into the genome at a desired locus, then Bxb1 installs the donor...and antibody-activator effector proteins; long-term imaging of proteins in living cells (Figure 9B) dCas9...

...four protein chains: two large heavy chains and two small light chains. These four domains come together...their monovalent nature. Darpins - Darpins are genetically engineered small proteins derived from ankyrin ...ankyrin repeat proteins. These are antibody mimics instead of modified antibodies and are not closely related...synthetic binding proteins which use the Fibronectin type III domain as their scaffolding instead of the antibody...Denature proteins, using heat and/or chemicals, and run on an SDS-PAGE gel to separate proteins by size...your antibody’s specificity. A blank (contains water or buffer instead of a biological sample), which captures... lab to specifically and tightly bind to other proteins. Antibodies, produced as part of the immune system...

...biofactories to express plasmids and produce proteins in high yields. Proteins can then be extracted from the culture...recombinant proteins to the periplasmic space between the inner and outer membranes. Targeting proteins to the...Plasmid Collection contains plasmids for non-toxic protein labeling and visualization inside living or fixed...Ligands GFP Fusion Proteins — Making the Right Connection Photoactivatable Fluorescent Proteins Fluorescent ...In BiFC, proteins of interest are fused to each part of a split FP. When the candidate proteins interact...Luciferase Fluorescent Biosensors Chromoproteins: Colorful Proteins For Molecular Biology Experiments ...Consortium page contains a variety of reagents for engineering and expressing zinc finger proteins. Addgene ...

...Firefly Insertion of 5' promoter/enhancer regions Joshua Mendell 60323 pGL4.23-GW Firefly Insertion of 5'...Firefly Insertion of 5' promoter/enhancer regions Bert Vogelstein 12178 pIS0 Firefly Insertion of target... Renilla Insertion of target sequence in the 3' UTR David Bartel 12177 pIS2 Renilla Insertion of target...construct that contains your regulatory element of interest. ID Plasmid Description Gene/Insert PI Return ...Troponin C Ca 2+ binding domain. Luminopsins : Luciferase-opsin fusion proteins for bioluminescent chemo-optogenetic...organisms, ranging from bacteria to aquatic animals to insects. Luciferase catalyzes the oxidation of a substrate...regions, or shRNA or CRISPR target sites can be inserted into a luciferase-containing plasmid and the effects...

... E MGC4638 INS insulin ILPR, IRDN INS-IGF2 INS-IGF2 readthrough transcript - INSL3 insulin-like 3 (Leydig...MGC119819, RLF, RLNL INSL4 insulin-like 4 (placenta) EPIL, PLACENTIN INSL5 insulin-like 5 MGC126695, MGC126697...MGC126697, PRO182, UNQ156 INSL6 insulin-like 6 RIF1 INSR insulin receptor CD220, HHF5 JAG1 jagged 1 (Alagille...communication between cells of the immune system. Interleukins Interleukins are cytokines that are secreted by immune... omega 1 - IGF1 insulin-like growth factor 1 (somatomedin C) IGF1A, IGFI IGF1R insulin-like growth factor...MGC18216 IGF2 insulin-like growth factor 2 (somatomedin A) C11orf43, FLJ22066, FLJ44734, INSIGF, pp9974 IGF2R...bA12D24.1.1, bA12D24.1.2 RLN3 relaxin 3 H3, RXN3, ZINS4, insl7 RNASE2 ribonuclease, RNase A family, 2 (liver...

...microbial opsins, such as the Halorhodopsins, Archaerhodopsins and Leptosphaeria rhodopsins, are pumps...encode proteins that are controlled by light. Many of these plasmids encode for microbial opsins, which...Find optogenetics plasmids such as microbial opsins and optical switch tools to activate, inhibit, and... opened and closed in response to light. These proteins can be expressed in specific neurons, often utilizing...in a precise manner. One of the first microbial opsins to be adopted as an optogenetic tool is the widely...anions into or out of the cell. When using these opsins as a tool to control neuronal activity, scientists...expression and localization. Many other types of proteins can also be controlled by light, generally by ...

...mCherry tagged fusion proteins in worms ins:MCS; cryaa:RFP - A zebrafish insulin promoter vector with ...designed for a user to insert a gene of interest. They may contain tags or fusion proteins, a multiple cloning...and pFastBac Dual LIC vectors for insect expression Fluorescent proteins (GFP, mCherry, etc) Localization...backbones curated by species, epitope tags/fusion proteins, function, selectable markers, and more. Plasmid...molecular biology to isolate, multiply, or express the insert they carry in the target cell. These vectors allow...function section for if you want to stably express an insert using viral vectors, are studying a different genetic...mammalian expression plasmid for cloning untagged proteins pcDNA3.1-mCherry - Expresses mCherry tag in mammalian...

...utilized CRISPR as a powerful defensive strategy against viral invaders, and this system is proving to be...genome in 1987, but their function as a safeguard against bacteriophages was not elucidated until 2007. Scientists...re-exposure. More specifically, specialized Cas proteins snip foreign DNA into small fragments approximately...stretches of DNA known as CRISPR arrays. Separate Cas proteins then express and process the CRISPR loci to generate...system. Within the bacterial genome, a CRISPR array contains many unique protospacer sequences that have homology... potential target sequence is only valid if it contains a special Protospacer Adjacent Motif (PAM) directly... varying PAM sequences and number/types of Cas proteins. Makarova et al. ’s classification defines 5 types...

...loops at tetraloop and stemloop - Contains BbsI sites for insertion of guide sequences Addgene plasmids...2 and EF1a-zeo resistance marker. Contains BsmBI sites for insertion of guide sequences Zhang Lab SAM ... microbial nuclease system involved in defense against invading phages and plasmids. CRISPR loci in microbial...nickase) + CRISPR RNA array + tracrRNA: This plasmid contains three expression cassettes. To target a given ...NGG PAM sequence. 3. SpCas9 alone: This plasmid contains a single cassette expressing human codon-optimized...2. SaCas9 only: This plasmid ( PX600, #61592 ) contains a single cassette expressing human codon-optimized...SpCas9 Cas9: The PX551 plasmid (AAV-SpCas9, #60957) contains a single cassette expressing human codon-optimized...

...Mullineaux Insulin Sensor for Imaging Insulin Secretion A Ratiometric Sensor for Imaging Insulin Secretion... Collections Fluorescent Proteins Fluorescent Biosensors Fluorescent Proteins: Biosensors Metal Ions Small...Additional Resources Fluorescent biosensors are proteins designed so that their fluorescent properties ...transfer (FRET) efficiency of a pair of fluorescent proteins. Genetically encoded biosensors can be introduced...Constitutive or Cre-dependent) Ultrasensitive fluorescent proteins for imaging neuronal activity. Nature. 2013 Jul...nested reporters of green- and orange-emitting proteins Ratiometric Matryoshka biosensors from a nested...cassette of green- and orange-emitting fluorescent proteins. Nat Commun. 2017 Sep 5;8(1):431. Wolf Frommer...

... monoclonal antibodies against zebrafish neural receptors and secreted proteins suitable for wholemount...of membrane proteins. Elife. 2018 May 24;7. and Generation of synthetic nanobodies against delicate proteins...Antibodies Antibody Plasmids Antibodies are large proteins made by the immune system that bind to an antigen...pull out a protein of choice from a mix of other proteins, visualize a protein under a microscope, or detect...in bacteria, mammalian cells, yeast, plants, or insect cells. Benefits include consistency between lots... Antibodies: Selecting and Evolving Functional Proteins. METHODS: A Companion to Methods in Enzymology...to rapidly engineer the constant immunoglobulin domains to obtain recombinant hybridomas, which secrete...

...nerve cell function within the cell. Parkinson's Disease Parkinson’s disease (PD) is a chronic and progressive...to highly conserved proteins and other difficult-to-target antigens in the Institute for Protein Innovation...More Fluorescent Proteins Find information on Addgene's collections of fluorescent proteins, including Markers...Differentiation Factors Antibodies Fluorescent Proteins Other Resources Neurodegenerative Disorders Neurodegenerative...neurodegenerative diseases include Alzheimer’s, Parkinson’s, ALS, and Huntington’s Disease. These diseases...with the mutated HTT gene will have huntingtin proteins with abnormally long polyglutamine sequences. ...organelles. (Mattia et al. EMBO Rep. 2018) Study intrinsically disordered protein regions with FUS and HNRNPA1...

...to boost the research focused on Solute carrier proteins (SLCs). Browse the SLC plasmid collection....Pooled Libraries Feedback Resources Solute carrier proteins (SLCs) are the largest family of transporters ...associations. Plasmids This plasmid collection contains codon-optimized ORF sequences for human SLCs and...developed by the RESOLUTE Consortium. ID Plasmid Gene/Insert Vector Type Industry PI Pooled Libraries CRISPR... libraries target the solute carrier family of proteins. Library ID PI Description Human SLC Activation... CRISPR activation library targeting human SLC proteins. Human SLC Knockout Library 132552 Superti-Furga...Superti-Furga CRISPR knockout library targeting human SLC proteins. Mouse SLC Knockout Library 141436 Superti-Furga...

...Plasmid Gene/Insert Vector Type PI Return to top Bacterial Strains Browse bacterial strains that have been...synthetase plasmids, target plasmids, and bacterial strains useful for expanding the genetic code in bacteria... Synthetase Plasmids Target Plasmids Bacterial Strains The genetic code for all life is based upon four...properties for studying, manipulating, and evolving proteins. Expanding the Genetic Code During protein translation...genetic code can help us understand and evolve proteins for various purposes from therapeutics to biopolymers... (RF1 is deleted); T7RNAP cassette genomically inserted Michael Jewett 182777 C321.∆A T7RNAP ompT- rne... (RF1 is deleted); T7RNAP cassette genomically inserted; ompT and rne inactivated Michael Jewett 182778...

...or MCP tagged plasmids for visualization of proteins inside cells....-, ACP-, and MCP-tags for visualization of proteins inside living or fixed cells. The plasmids associated... labeling cell-surface proteins, and are useful for labeling secreted proteins with disulfide bridges ...features: Allows for visualization of multiple proteins simultaneously with dual labeling when used with...live or fixed cells, and for internal or surface proteins Single constructs are compatible with multiple... and MCP-tags are used exclusively for surface proteins and use enzymatic binding to attach substrate ...suitable for both cell-permeant and non-cell-permeant proteins. CLIP-tag CLIP-tag is identical in function to...

...plasmid — contains transgene, sgRNA, or shRNA of interest flanked by LTRs Packaging plasmid — contains packaging...plasmids in third-generation Envelope plasmid — contains packaging gene env This collection highlights ...1alpha-driven constitutive transgene expression. Contains intron that gives high expression. Didier Trono...and Paul Kaufman 102930 pLJC5-Tmem192-3xHA 3rd Contains a lysosomal tag. See Abu-Remaileh et al. (2017...expression of GFP. Jason Sheltzer 20342 FUW-M2rtTA 3rd Contains reverse tetracycline transactivator. Rudolf Jaenisch...and rev . Garry Nolan 8455 pCMV-dR8.2 dvpr 2nd* Contains gag , pol , rev , tat , vif , and nef . Can be...information. Bob Weinberg 12251 pMDLg/pRRE 3rd Contains gag and pol . Can be used with 3rd generation ...

... Gene/Insert Promoter Selectable Marker PI Publication Return to top Cut ID Plasmid Gene/Insert Promoter...Plasmid Gene/Insert Selectable Marker PI Publication Return to top Interfere ID Plasmid Gene/Insert Promoter...Gene/Insert Promoter Selectable Marker Publication Return to top Prime Edit ID Plasmid Gene/Insert Promoter... Gene/Insert Selectable Marker PI Publication Return to top RNA Knockdown ID Plasmid Gene/Insert Promoter...table for "Mammalian". Activate ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return...Publication Return to top Purify ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return...Publication Return to top Visualize ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return...

...small tag insertions, by copying from a DNA donor template Cas9 or Cas9 fusions with proteins that promote...including point mutations, base changes, and large insertions or deletions. For more information on the wide...our CRISPR Guide . There are many different Cas proteins available, and researchers can adapt these experiments...Cas9 nickase fusions with different base editing proteins) Many base editors fused to Cas9 exert their activity...sequence Base Edit Prime Edit Generate targeted insertions, deletions, and point mutations using a prime... In many cases, you can synthesize, anneal, and insert the targeting oligos into plasmids containing the...can generate stable cell lines if your plasmid contains a selection marker. Plasmid delivery is versatile...

...Gene/Insert Promoter Selectable Marker PI Publication Return to top Bacteria ID Plasmid Gene/Insert Promoter...Gene/Insert Promoter Selectable Marker PI Publication Return to top Plant ID Plasmid Gene/Insert Promoter...Gene/Insert Promoter Selectable Marker PI Publication Return to top Yeast ID Plasmid Gene/Insert Promoter...Gene/Insert Promoter Selectable Marker PI Publication Return to top Xenopus ID Plasmid Gene/Insert Promoter...joining (NHEJ), a mechanism which frequently causes insertions or deletions (indels) in the DNA. Indels often...repair a DSB using homology-directed repair (HDR) instead of NHEJ. In most experimental systems, HDR occurs...Publication Return to top Parasite ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return...