We narrowed to 482 results for: vent
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TypeBlog Post...digestion, you will need to use a phosphatase to prevent re-circularization of the backbone plasmid (see...
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Quick Guide to All Things Lentivirus
TypeBlog Post...containing the psi deletion). A double crossover event between the vector plasmid and the packaging plasmid... -
RNA Interference in Plant Biology: New Tools for an Old Favorite
TypeBlog Post...growth in the lab of Luis Vidali. For his next adventure, he will join the lab of Darren Gilmour at the... -
9 tips for a successful postdoctoral experience
TypeBlog Post... the end of grad school, you searched for and eventually identified a postdoctoral mentor. A good mentor... -
Which Fluorescence Microscopy Technique is Best for Me?
TypeBlog Post...also excel on these types of samples. Prior to the advent of super-resolution imaging, the light microscope... -
A Primer on Optogenetics: Introduction and Opsin Delivery
TypeBlog Post...to express. For example, if you inject into the ventral tegmental area (VTA) but wish to stimulate VTA ... -
Antibody Guide
TypeGuide...block the membrane to remove excess antibody and prevent unwanted binding. Incubate the membrane with a ... -
Selecting Your Plasmid Purification Kit
TypeBlog Post...purification kits make it easier than others to prevent quality issues. For example, some kits reduce the... -
A Needle in a Base-Stack: Cas9 Structural Biology
TypeBlog Post...lobes are inactive in the absence of guide RNA, preventing Cas9 from making off-target cuts. Pro tip! ... -
Advice on Career Paths and the Green Card Process for International Researchers and Entrepreneurs
TypeBlog Post...IT, boutique consulting, investment banking and venture capital areas. We place individuals into roles... -
The CRISPR Software Matchmaker: A New Tool for Choosing the Best CRISPR Software for Your Needs
TypeBlog Post...example is CRISPR-GA. These tools assess repair events by type (NHEJ/HDR) and track indels at a single... -
Kit Free RNA Extraction
TypeProtocol...and hazards for these reagents. Work in a well-ventilated space and under a fume hood when working with... affects the solubility of the RNA. Pro-Tip To prevent overdrying, watch the pellet and carefully remove...for RNA. Store your RNA sample(s) at -80 °C to prevent RNA degradation and avoid multiple freeze-thaw ... for RNA.Store your RNA sample(s) at -80 °C to prevent RNA degradation and avoid multiple freeze-thaw ... -
Deep Dive: qPCR
TypeBlog Post...transcripts. However, adding too many reagents can prevent your reaction from reaching the plateau stage after... -
CRISPR/Cas9 FAQs Answered!
TypeBlog Post...makes sense that scientists, many of whom are venturing into genome editing for the first time, have lots... -
CRISPR Methods for Bacteria: Genome Engineering, CRISPRa, CRISPRi, Base Editing, and More
TypeBlog Post... in the Cas9 or spacer sequence, and these rare events are easily detectable using PCR. The system also... -
15 Hot Plasmids from 2017
TypeBlog Post... the point mutations created by Agudelo et al. prevent ouabain binding to ATP1A1 but do not compromise... -
Technical Design of a Western Blot
TypeBlog Post... digitized for quantitative work — and with the advent of online lab notebooks, it’ll likely need to be... -
Weighing Reagents Protocol
TypeProtocol...might need to weigh it out in the fume hood to prevent exposure. Read the reagent’s material safety data...used to contain the material you’re weighing and prevent cross-contamination with other substances. They...spatula is clean, and sterile (if necessary), to prevent any potential contamination. Scoop out the material... -
Water Bath Protocol
TypeProtocol...water bath with distilled water, not tap water, to prevent salts from accumulating on the surfaces of the ...evaporates from the tub. Disinfectant may be added to prevent growth of bacteria or fungi. There are disinfectants...water bath cover on the top of the water bath to prevent evaporation and maintain the desired temperature... -
Gibson Assembly Protocol
TypeProtocol...Dr. Daniel Gibson and colleagues at the J. Craig Venter Institute developed a novel method for the easy...Biolabs by TelesisBio. Gibson DG, Young L, Chuang RY, Venter JC, Hutchison CA, Smith HO. (2009). Enzymatic assembly... new window) Gibson DG, Smith HO, Hutchison CA, Venter JC, Merryman C. (2010). Chemical synthesis of the...