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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 677 results for: RON

Showing: 231 - 240 of 677 results

  1. Donations from Addgene to Yield Answers for Rare Disease Researchers

    Type
    Blog Post
    ...leaves patients incredibly sensitive to sunlight and prone to developing metastatic melanomas and other cancers...,” McSweeney said. “But, it is still sometimes wrongly diagnosed as a seizure disorder.” In 2012, researchers...sodium-potassium pump expressed specifically in neurons of the brain. McSweeney says they now think that...that mutations in the gene may prevent neurons from returning to basal conditions normally after periods...

  2. New and Upcoming Viral Vectors - June 2019

    Type
    Blog Post
    ...improved detection for tracking large populations of neurons in wide-field imaging (Dana et al., 2018). See ...Plasmid Serotype Name 119036  AAV1  pAAV-hsyn-flex-Voltron-ST DREADDs / Chemogenetics AAV We have also ...chemogenetic AAV for activating and inhibiting neurons. Minimally-tagged DREADD AAV from the Richie lab...with Cre for intersectional control of specific neuronal populations. We now offer a Flp-expressing AAV...

  3. 25 Work From Home Tips for Scientists

    Type
    Blog Post
    ...photos of our pets. #SocialDistancing may send a wrong message for some people. We should recommend #PhysicalDistancing...the COVID-19 pandemic, check out Care For Your Coronavirus Anxiety and Taking Care of Your Behavioral Health...home, the STEM career exploration site Inventing Heron has a radio reporter challenge, and code.org has...necessarily to chat, but to mimic your usual work environment.  Be kind to yourself. This is a challenging ...

  4. What Good Citizenship Can Do for Reproducibility in Science

    Type
    Blog Post
    ... chow, a different water filtration system, environmental stability in an incubator), but there’s a lot...for a transparent and collaborative research environment. Journals as enforcers Currently, most of the... formatting can feel like yet another hurdle in front of publication when all they really want to do is... of incentives. Right now, researchers are not strongly rewarded for being transparent in their communication...

  5. Resume Writing for Non-academic Science Careers

    Type
    Blog Post
    ...presented chronologically. With a resume however, most often people choose a reversed chronological style ...you did. Emphasize the skills. If you studied astronomy or bioinformatics data, then the chance is that...Communications Coordinator for the Department of Physics & Astronomy, University of British Columbia. Originally on...

  6. 15 Years of Addgene: The Top 15 Plasmids

    Type
    Blog Post
    ...generation lentiviral packaging plasmid from the Didier Trono lab that expresses the HIV gag, pol, rev, and tet...pMD2.G. Find psPAX2. pMD2.G - Also from the Didier Trono lab is pMD2.G, a VSV-G envelope expressing plasmid...generation lentiviral packaging plasmid from the Trono lab contains the rev gene. It’s meant to be used...generation lentiviral packaging plasmid from the Trono lab that contains the gag and pol genes. It also...

  7. Hot Plasmids - May 2022

    Type
    Blog Post
    ...flexibility for in vivo proximity labeling Increased neuronal expression using Cre-mediated AAV Targeted Evolution...flexibility for in vivo proximity labeling   By Ashley Waldron Proximity-dependent labeling has become a powerful...doi.org/10.1038/s41593-021-00969-4.   Increased neuronal expression using Cre-mediated AAV Targeted Evolution...found at least two variants that display increased neuronal expression in the brain while having decreased...

  8. Viral Vectors 101: Biosensors

    Type
    Blog Post
    ...The calcium is strong here….I can sense it….sound like a plot to an biology scifi movie? Or maybe it’...contraction, and calcium channels are activated for neuronal action potentials; it’s an ion of interest for...monitor calcium specifically in their favorite neurons. This specificity is feasible partially because...Neuromodulation: Engineering and Applications. Frontiers in Cellular Neuroscience. 2019 Oct Vol. 13; 1662...

  9. Plasmids 101: Blue-white Screening

    Type
    Blog Post
    ...beginning. The well-characterized bacterial lac operon contains a gene called lacZ that encodes for the...the enzyme β-galactosidase. Expression of the lac operon is induced by lactose, and also by a lactose analogue...completely accurate, IPTG binds and inactivates the lac operon repressor, thereby allowing lac expression). When...in the medium to ensure transcription of the lac operon.   Tips for blue-white screening Use a good control...

  10. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

    Type
    Blog Post
    ...separated by 28 base Csy4 recognition sites from a polycistronic transcript. Although this method shortens construct...requires coexpression of Csy4 with Cas9 and the polycistronic transcript. As nucleases may have off-target... of gRNA cleavage.   Xie et. al constructed polycistronic glycine tRNA-gRNA genes (PTGs) in the forms ...Bacteria and viruses have illustrated the power of polycistronic gene organization, and its translation to CRISPR...
Showing: 231 - 240 of 677 results