We narrowed to 569 results for: CIT

1. Top Requested Lentivirus and AAV of 2016

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   Blog Post

   ...high titer Cas9 lentiviruses challenging. This cytotoxicity also causes growth rate variability when using...

2. Hot Plasmids - June 2019 - Optogenetics, Acoustic Reporter Genes, microRNAs, and the CRISPR-Cas9 system CHIME

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   ...engineered cells in vivo, the non-immunogenic violet-excited GFP variant Vex. To understand the impact of this...

3. Synthesized by Ginkgo Bioworks, Shared by Addgene: SARS-CoV-2 Plasmids for Many Expression Systems

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   ..., please fill out this form and we’ll add your citation to the plasmid page on our website. Contributing...

4. Hot Plasmids - February 2022

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   ...genes within a single cassette provides increased simplicity for combinatorial genetic screens, and this optimized...

5. Antibodies 101: Isotypes

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   ...structure, which means each IgM molecule has a binding capacity or valency of 10 antigens. An extra constant domain...

6. Viral Vectors 101: Biosensors

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   ...specifically in their favorite neurons. This specificity is feasible partially because of viruses—keep...

7. GCE4All: Making Genetic Code Expansion Accessible

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   ...ncAA protocol, to optimize and share, they’re soliciting feedback from scientists on which protocols would...

8. From Lab Bench to Leadership: Transitioning into Management as a PhD

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   Blog Post

   ...the leap into management, the journey can be as exciting as it is challenging. How do you prepare for...

9. Viral Vectors 101: Gamma-Retroviral Packaging Systems

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   ... of a retroviral provirus (see below). Cloning capacity between the LTRs is ∼8.5 kb, but inserts bigger...

10. How Addgene Creates Content

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    Blog Post

    ... in BioRender or use appropriately licensed and cited figures from papers or image sharing repositories...

11. Plasmids 101: Screens vs. Selections

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    ...genes are assayed for their role in a particular capacity. Drug screens, on the other hand, are often performed...

12. Developing a cold tolerant E. coli using specialized chaperones

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    ...SynBio arena this year with a fresh team of twenty excited undergrads. Team IISER-B’s idea for the iGEM 2019...

13. Opto-Nanobodies: Using Light to Manipulate Cell Signaling and Protein Purification

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    Blog Post

    ...control of signaling pathways. This is particularly exciting because using light to control signaling provides...

14. A Deep Dive into BioBuilder

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    Blog Post

    ...create a program that would capitalize on the excitement found through experiential learning. “Students...

15. Protein Tagging with CRISPR/Cas9: A Conversation with Mendenhall and Myers

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    ...Spring Harbor in March. A number of groups were excited and asked for the protocol. Since the Addgene page...

16. 3 Challenges in Plant Synthetic Biology

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    ...the repository, MoClo and GreenGate, and we are excited to see how this exchange of genetic tools facilitates...

17. Hot Plasmids April 2018 - Protein Degradation, Nanoscopy, FIRE-Cas9, and Yeast Expression Tools

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    ...results to rapamycin inhibition. To assess TRIM21 toxicity, Clift et al. created stable cell lines overexpressing...

18. Visualizing Protein Turnover In Situ

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    ...specific endogenous proteins in any cell type. Exciting! Many thanks to our guest blogger, Eugenia Rojas...

19. Choosing the B(right)est Fluorescent Protein: Aggregation Tendency

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    Blog Post

    ... the aforementioned methods only determine the capacity of homodimerization in aqueous solution. In cyto...

20. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

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    ...through the loss of the F plasmid, succumb to the toxicity of CcdB.   Figure 1: Image from Bernard...