We narrowed to 464 results for: LARGE

1. Isolating a Monoclonal Cell Population by Limiting Dilution

   Type

   Protocol

   ...over-confluent, trypsinize the cells and expand them to larger culture dishes, being sure not to cross contaminate...

2. CRISPR Library Amplification

   Type

   Protocol

   ...libraries up to 200,000 individual plasmids. For larger libraries scale the number of cells and DNA accordingly...

3. Cloning

   Type

   Guide

   ...overlapping homology. One major advantage is that much larger final products can be generated (up to 100kb) compared... progressively more difficult to clone plasmids larger than 10kb. Another advantage is the ability to ...

4. Chemogenetics Guide

   Type

   Guide

   ... on G-protein coupled receptors, or GPCRs. The largest class of cell surface receptors, GPCRs are seven-pass...