We narrowed to 469 results for: LARGE

1. Weighing Reagents Protocol

   Type

   Protocol

   ...places. Pro-Tip If you’re weighing out an amount larger than the capacity of your balance or weighing boat...

2. Protocol - How to Ligate Plasmid DNA

   Type

   Protocol

   ...proportionally. 1μL of ligase should be sufficient for larger ligation reactions. Because ligase buffer contains...

3. Protocol - How to Run an Agarose Gel

   Type

   Protocol

   ..., and a lower percentage gel will help separate larger bands. 10% Rule For each sample you want to load...

4. Handling Plasmids from Addgene - Purifying Plasmid DNA

   Type

   Protocol

   ... of bacteria to grow (low copy plasmids require larger cultures). Centrifuge the culture to pellet the...

5. Virus Protocol - Generating Stable Cell Lines

   Type

   Protocol

   ... individual wells become confluent, expand into larger vessels. A confluent well of a 6-well dish can ...

6. Isolating a Monoclonal Cell Population by Limiting Dilution

   Type

   Protocol

   ...over-confluent, trypsinize the cells and expand them to larger culture dishes, being sure not to cross contaminate...

7. CRISPR Library Amplification

   Type

   Protocol

   ...libraries up to 200,000 individual plasmids. For larger libraries scale the number of cells and DNA accordingly...

8. Cloning

   Type

   Guide

   ...overlapping homology. One major advantage is that much larger final products can be generated (up to 100kb) compared... progressively more difficult to clone plasmids larger than 10kb. Another advantage is the ability to ...

9. Chemogenetics Guide

   Type

   Guide

   ... on G-protein coupled receptors, or GPCRs. The largest class of cell surface receptors, GPCRs are seven-pass...