Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 482 results for: vent

Showing: 461 - 480 of 482 results

  1. Pouring LB Agar Plates

    Type
    Protocol
    ...concentration disolved in the appropriate liquid solvent. See table to the right for appropriate antibiotic...bottles. The extra empty volume is necessary to prevent your molten agar from boiling over in the autoclave...psi for at least 30 min. The high pressure will prevent your gel mix from boiling over at high temperature...

  2. Protocol - How to Create a Bacterial Glycerol Stock

    Type
    Protocol
    ...addition of glycerol stabilizes the frozen bacteria, preventing damage to the cell membranes and keeping the ...stock on dry ice while streaking onto LB agar will prevent it from thawing completely and will improve the...

  3. Fluorescence Titering Assay

    Type
    Protocol
    ... cells. Titering methods assume one integration event per cell. When the percentage exceeds 40% you risk...risk counting cells with multiple integration events leading to underestimation of the true titer. Calculate...

  4. Protocol - How to Ligate Plasmid DNA

    Type
    Protocol
    ...insert will be added in the correct orientation and prevents the vector from ligating to itself during the ...phosphatase removes the 5' phosphate and therefore prevents the ligase from being able to fuse the two ends...

  5. Pipetting Protocol

    Type
    Protocol
    ... needed to prevent contamination. When not in use, the tip box should be closed to prevent contamination...

  6. Virus Protocol - Generating Stable Cell Lines

    Type
    Protocol
    ...may also exhibit varying numbers of integration events. In other words, if an MOI >1 was used, some cells...rates, especially if that transgene is toxic. Eventually, the rapidly growing low-level transgene expressors...

  7. 27 Hot Plasmids from 2016

    Type
    Blog Post
    ...and a repair template for an individual tagging event. Commonly used sequences, including fluorescent ...

  8. DNA Quantification

    Type
    Protocol
    ...the background inherent to the machine and your solvent. If using a NanoDrop to measure your samples, place...

  12. General Transfection

    Type
    Protocol
    ... time, allow them to mix and recheck the pH to prevent over or undershooting the desired pH. Allow the...

  13. Lentivirus Production

    Type
    Protocol
    ... time, allow them to mix and recheck the pH to prevent over or undershooting the desired pH. Allow the...

  14. Colony Formation Titering Assay

    Type
    Protocol
    ...titers as the likelihood of multiple integration events decreases as the dilution increases. Calculate ...
Showing: 461 - 480 of 482 results