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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 677 results for: RON

Showing: 241 - 250 of 677 results

  1. Rosella: A Fluorescent pH-Biosensor for Studying Autophagy

    Type
    Blog Post
    ...autophagy. Morphological assays Electron microscopy: Electron microscopy is a traditional method for...at various stages of degradation. Additionally, electron microscopy analysis is time consuming. GFP-tagged...will fluoresce green unless it’s in an acidic environment like the vacuole or lysosome. See Table 2 for...

  2. Plasmid Tools for Microbiome Studies

    Type
    Blog Post
    ...difficult to culture outside of their natural environment. Thus, the Harris Wang lab at Columbia University...gut microbiome members in their native habitat (Ronda et al., 2019). They called their method Metagenomic...19465049. PubMed Central PMCID: PMC2752045. Myhrvold, Cameron, et al. "A distributed cell division counter reveals...PMID: 26615910. PubMed Central PMCID: PMC4674677. Ronda, Carlotta, et al. "Metagenomic engineering of the...

  3. Hot Plasmids April 2018 - Protein Degradation, Nanoscopy, FIRE-Cas9, and Yeast Expression Tools

    Type
    Blog Post
    ...the appropriate ligand - estradiol for hER or progesterone for hPR - leads to nuclear localization, binding...controlled transcription factor and a ZIF268/progesterone controlled transcription factor as well as reporters...with various concentrations of estradiol and progesterone and showed that reporter expression is dependent...cognate inducer. Since induction via estradiol and progesterone do not affect each other, these systems can ...

  4. Antibodies 101: Conjugation

    Type
    Blog Post
    ...capable of producing a visible signal, but it has a strong affinity for avidin and streptavidin, both of which...modifying the antibody sequence or that rely on strong protein-protein interactions with other peptides...of non-canonical amino acids or peptide tags. 5) Strong protein-protein interactions with other peptides... Ashley Waldron...

  5. Gendered Innovations: Why Does Sex of the Cell Matter?

    Type
    Blog Post
    ...sex and gender analysis important? Doing research wrong costs lives and money. According to the US General...treated, and other hormonal, immunological, and environmental factors. How to include sex in experimental ...Science, Health & Medicine, Engineering, and Environment—a project aimed at harnessing the creative power...Evoloving Check Out Extracurricular Activities for a Strong Science Career Path Additional Resources Check...

  6. CRISPR 101: Cytosine and Adenine Base Editors

    Type
    Blog Post
    ...effective at editing cytosines preceded by guanine (Thuronyi et al., 2019). Following the development of adenine...created a dual-deaminase editor called SPACE (synchronous programmable adenine and cytosine editor) by ...high product purity and low off-target editing Thuronyi et al., 2019 evoAPOBEC1-BE4max   Improved editing... K. T., Eton, E., Lapinaite, A., Newby, G. A., Thuronyi, B. W., Wilson, C., Koblan, L. W., Zeng, J., Bauer...891. https://doi.org/10.1038/s41587-020-0453-z Thuronyi, B. W., Koblan, L. W., Levy, J. M., Yeh, W.-H....

  7. Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation

    Type
    Blog Post
    ...and Memory Formation. Neuron 79, 6 P1086-1093. https://doi.org/10.1016/j.neuron.2013.08.032.  Morita S...enzymatic fusions, such as dCas9-TET, can, like the iron ball-and-chain that they resemble, actually impede...unmethylated CpG to behave naturally in the nuclear environment. One good system to balance these needs is the...

  8. Hot Plasmids February 2024

    Type
    Blog Post
    ...in green monkeys and cultured human neurons, with broader neuronal expression than AAV9. This new capsid...-brain barrier and delivering a transgene, with neuron bias observed in young macaques and vascular bias...Recombinant anti-FLAG tag antibodies By Ashley Waldron Of all the epitope tags we know and love, the FLAG...

  9. The Materials Science of Optogenetics Experiments

    Type
    Blog Post
    .... Pulse generation (TTL pulse) is a standard electronics protocol not specific to lasers or optogenetics... Use of channelrhodopsin for activation of CNS neurons. Current protocols in neuroscience / editorial ... Kim TI, et al. Injectable, cellular-scale optoelectronics with applications for wireless optogenetics... et al. A simple head-mountable LED device for chronic stimulation of optogenetic molecules in freely ...

  10. How-to: 5 Steps to a Great Panel Discussion

    Type
    Blog Post
    ... relaxed phone call than when first confronted on a stage in front of a crowd. The moderator will then...logistics To make a really lively discussion, I strongly suggest asking the panelists not to use any slides... turnout). This gets people to sit close to the front so the interactions are better. Have a good microphone...
Showing: 241 - 250 of 677 results