An Introduction to Adenovirus
Type
Blog Post
...kilobases; compared to only 4.7 kb for AAV, 9 kb for retroviruses). The tradeoff is that adenoviral vector expression...
We narrowed to 601 results for: RET
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Course-Based Undergraduate Research Experiences (CUREs)
TypeBlog Post...Zuckerman, 1977; Ma et al., 2020). Many have interpreted this trend as evidence that the best teachers... -
Uncovering Ancestral Bias in CRISPR Research
TypeBlog Post...and consider bias as a confounding factor when interpreting findings, particularly when researching ancestry... -
SciComm: Taking Science to Elementary and Middle Schools
TypeBlog Post... itself in terms of what they do with their discretionary money. They also usually have a good event-planning... -
Isolating Genomic Regions of Interest with the CRISPR System
TypeBlog Post...Fujii Lab generated a transgenic mouse line and retroviral expression system for using this approach in ... -
AAVs CREATed for Gene Delivery to the CNS and PNS
TypeBlog Post...Multi-color labeling of neurons is for more than making pretty pictures (see figure 4). It also enables single-cell... -
DNA Purification Without a Kit
TypeBlog Post...but trace contamination could interfere with interpreting the results of transfection assays. Go forth... -
Better Dyeing Through Chemistry & Small Molecule Fluorophores
TypeBlog Post... try out the JF dyes. Acknowledgements I thank Brett Mensh, a wordsmith soldier of fortune, for helpful... -
Lentiviral Vector Uses and Overview
TypeBlog Post...researchers developed viral vector systems based on retroviruses like Moloney murine leukemia virus (MMLV). The... -
Minigenomes - a Safe Way to Study Dangerous Viruses Like the Ebola Virus
TypeBlog Post...independently of other viral proteins simplifying the interpretation of results. This system can be used to develop... -
Cpf1: A New Tool for CRISPR Genome Editing
TypeBlog Post...cleaves relatively far away from the PAM, NHEJ might retain the PAM site. Therefore, if HDR does not initially... -
Negotiating Work and Life: How to Find the Joy
TypeBlog Post...running errands. Give help when you have time, and in return you will get help when you need it. On the work... -
"Hall of Fame" AAV Enhancers from the Allen Institute for Brain Science
TypeBlog Post... vectors that can be administered systemically (retro-orbitally, RO; or intracerebroventricularly, ICV... -
Harnessing TnpB for Genome Editing: A Compact and Versatile Tool for Your Research
TypeBlog Post...doi.org/10.1038/s41587-019-0337-2 Molla, K. A., Sretenovic, S., Bansal, K. C., & Qi, Y. (2021). Precise ... -
QC Sequencing Technologies at Addgene
TypeBlog Post...for discrepancies in backbone sequences. Most theoretical sequences submitted to Addgene have minor differences... -
Tips and Tricks for Using Golden Gate Modular Cloning (MoClo)
TypeBlog Post...stocks. To avoid cross-contamination, we recommend retrieving plasmids from your original Addgene kit plate... -
CRISPR 101: Any Base Transversion Editors
TypeBlog Post...adenine transversion editor AYBEv3. This tool still retained considerable A → G editing activity at most sites... -
Twenty Years of Sharing: Expanding Accessibility
TypeBlog Post...Lentiviral Guide AAV Guide Optogenetics Guide γ-Retrovirus Guide Protocols Agarose Gel Electrophoresis... -
Twenty Years of Sharing: Addgene's Viral Vector Service
TypeBlog Post...vectors in a variety of serotypes, including the retrograde AAVrg and the systemic capsid PHP.eB. ... -
Antibodies 101: Reading a Flow Plot
TypeBlog Post...fluorescent signals, and how the machine reads/interprets the signals, the axis is instead divided into...