We narrowed to 618 results for: Ret;

...https://doi.org/10.1128/mbio.00019-20 Singh AK, Carette X, Potluri L-P, Sharp JD, Xu R, Prisic S, Husson...

... of ~50% and confirmed that the fusion protein retained the PAM properties of the individual Cas9. They...

...dimerization mTagBFP2-pBAD - Bacterial Expression Return to top Cyan Protein Excitation (nm) Emission (nm...Mammalian Expression MiCy-pBAD - Bacterial Expression Return to top Green Protein Excitation (nm) Emission (...mNeonGreen-C Cloning Vector - Mammalian Expression Return to top Yellow Protein Excitation (nm) Emission ...Bacterial Expression mCyRFP1-C1 - Mammalian Expression Return to top Orange Protein Excitation (nm) Emission ...Expression tdTomato-pBAD - Bacterial Expression Return to top Red Protein Excitation (nm) Emission (nm...- Mammalian Expression pMXs-mRFP1 - Mammalian Retroviral Expression mCherry 587 610 16 4.5 15 min Monomer...Mammalian Expression mCherry2-C1 - Mammalian Expression Return to top Far-Red Protein Excitation (nm) Emission...

...thus preserving physiological relevance. NanoBRET Assays NanoBRET (Link opens in a new window) is an energy...proximity in living cells. NanoBRET PPI assays use NanoLuc Luciferase as a BRET energy donor and HaloTag...window) and NanoBRET assays (Link opens in a new window) NanoLuc®, HaloTag®, NanoBiT®, and NanoBRET® are Promega...Promega collection are available in two formats: pretransformed in bacteria as an agar stab 20 µg purified ...HaloTag protein, labeled with the NanoBRET 618 fluorophore, as the energy acceptor. Live-cell reagents allow... time using full-length proteins or fragments. NanoBRET target engagement (TE) assays (Link opens in a...compounds is measured by competitive displacement of a NanoBRET tracer, reversibly bound to a NanoLuc luciferase-fusion...

...Plasmid Description Industry Return to top Blue Plasmid Description Industry Return to top Cyan Plasmid Description...Description Industry Return to top Green Plasmid Description Industry Return to top mEmerald Plasmid Description...Description Industry Return to top Yellow Plasmid Description Industry Return to top Orange Plasmid Description...Description Industry Return to top Red Plasmid Description Industry Return to top Photoactivatable Including...fluorescent proteins. Plasmid Description Industry Return to top Content last reviewed: 25 September 2025...

... Publication Return to top Bacteria ID Plasmid Gene/Insert Promoter PI Publication Return to top Drosophila...Publication Return to top Plant ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top...Publication Return to top Yeast ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top...Publication Return to top Xenopus ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top...Insert Promoter Selectable Marker PI Publication Return to top CRISPR Resources Addgene has a large selection...

...PGKpuro2ABFP - For retroviral delivery of one sgRNA pMKO.1 GFP - Retroviral shRNA expression Retroviral backbones...Zebrafish Plasmids and Resources collection page Return to top Epitope Tag or Fusion Protein Tags and fusion...bacterial expression (Gateway) pYD1 - Expression, secretion, and display of proteins on the extracellular ...as TEV cleavable N-terminal His and NusA fusions Return to top Function Besides functions such as protein...expression, see our dedicated Lentiviral Plasmids page Retroviral Easy and safe to use, integrate into host genome...MSCV-IRES-GFP or pMSCV-IRES-mCherry FP - Mammalian retroviral gene expression with GFP or mCherry selectable...mammalian cells with the Tet-On or Tet-Off system Return to top Selectable Markers Regardless of your delivery...

...nickase) and single guide RNA 48137 : PX165; SpCas9 Return to top GeCKO Screening Library GeCKO are pooled...for more information. Full references are below. Return to top Cas9 SAM transcriptional activation plasmids...sequences Zhang Lab SAM Cloning Protocol 321.5 KB Return to top AAV - In vivo Genome Editing The Zhang Lab...fusion facilitates FACS sorting of cells and nuclei. Return to top AAV - Cas9 mouse CRISPR-Cas9 is a versatile...designed based on the target site sequence (20bp). Return to top References SpCas9 plasmids Multiplex Genome...CRISPR/Cas Systems. Cong L, Ran FA, Cox D, Lin S, Barretto R, Habib N, Hsu PD, Wu X, Jiang W, Marraffini ...10.1038/nprot.2013.143. Epub 2013 Oct 24. PubMed . Return to SpCas9 plasmids GeCKO Library Genome-scale CRISPR-Cas9...

...Promoter PI Publication Return to top Base Edit ID Plasmid Promoter PI Publication Return to top Cut ID Plasmid...Publication Return to top Interfere ID Plasmid Gene/Insert Promoter PI Publication Return to top Nick ...Publication Return to top Prime Edit ID Plasmid Gene/Insert Promoter PI Publication Return to top Purify...j.bbrc.2013.08.013. Return to top RNA Targeting ID Plasmid Promoter PI Publication Return to top CRISPR Resources...

...MMLV-derived Retrovirus Human Yamanaka factors for generating human iPS cells; retroviral expression of...MMLV-derived Retrovirus Mouse Original set of Yamanaka factors for generating mouse iPS cells; retroviral expression..., fully-differentiated adult somatic cells are returned to a pluripotent state using a cocktail of factors...11(3):373-86. Jaenisch Fibroblasts Hepatocytes Retroviral Mouse Direct conversion of mouse fibroblasts ...nature10323. Crabtree Fibroblasts Motor Neurons Retroviral Mouse/Human Conversion of mouse and human fibroblasts...2527-32. Wernig Fibroblasts Neural Stem Cells Retroviral Mouse Direct reprogramming of fibroblasts into...Cell Stem Cell. 2019 Jun 6. Buganim Glial Cells Retinal Ganglion Cells AAV/CRISPR Mouse Glia-to-Neuron ...

...Cre recombinase. Takuji Iwasato 63704 pRetroX GFP T2A Cre Retroviral vector for dox-inducible expression.... tTA-Advanced Tight TRE David Sabatini 19407 pTREtight2 Empty backbone for Tet-controlled expression ...promoters. None Pbi (TRE, miniCMV) Bert Vogelstein Return to Top Transactivators (tTA or rtTA) Find a construct...promoter rtTA-Advanced Rudolf Jaenisch 25434 pMA2640 Retroviral Tet-On vector for CMV-driven rtTA with EGFP and...from the hSyn promoter tTA-Advanced Takeshi Imai Return to Top Other Tet Applications Explore some other...monosynaptic tracing. To be coinjected with pAAV-TREtight-mTagBFP2-B19G ( Plasmid #100799 ). Ian Wickersham...dox-inducible H2B-mCherry marker. Aviv Regev , Joan Brugge Return to Top Additional Resources Blog: Inducible Promoters...

...floxed-shRNA constructs, Cre can excise the shRNA to return gene expression to physiological levels. In lox-STOP-lox...Flp-dependent expression systems and may affect the interpretation of your experimental results. Attenuated iCre...of Cre-lox and Flp-FRT — A Neuroscientist’s View Return to top Flp Recombinase Plasmids ID Plasmid Description...of Cre-lox and Flp-FRT — A Neuroscientist’s View Return to top Dre Recombinase Plasmids ID Plasmid Description...expressing Dre and other site-specific recombinases. Return to top Other Site-Specific Recombinase Plasmids...Description Gene/Insert Vector Type Promoter PI Return to top Recombinase-Dependent Empty Backbones Find...find other Cre-, Flp-, or Dre-dependent plasmids. Return to top Recombinase-Dependent Reporter Plasmids ...

...Publication Return to top Cut ID Plasmid Gene/Insert Selectable Marker PI Publication Return to top Interfere...Publication Return to top Nick ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top...Insert Promoter Selectable Marker PI Publication Return to top Base Edit ID Plasmid Gene/Insert Promoter...Insert Promoter Selectable Marker PI Publication Return to top CRISPR Resources Addgene has a large selection...

...Publication Return to top Cut ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top...Publication Return to top Purify ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top...Insert Promoter Selectable Marker PI Publication Return to top Base Edit ID Plasmid Gene/Insert Promoter...Insert Promoter Selectable Marker PI Publication Return to top Nick ID Plasmid Description Gene/Insert ...

... Publication Return to top Bacteria ID Plasmid Gene/Insert Promoter PI Publication Return to top Drosophila...Publication Return to top Plant ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top...Publication Return to top Yeast ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top...

...antibodies targeting families of cell surface and secreted proteins from a collaboration between Addgene ... Isotype Reactivity Recommended Applications PI Return to top Synaptic Cleft IPI’s Synaptic Cleft Antibody... Isotype Reactivity Recommended Applications PI Return to top Integrins Integrins are a family of mechanotransduction...Description Isotype Reactivity Recommended Applications Return to top Browse Epitope Tag Antibodies IPI has developed...Description Isotype Reactivity Recommended Applications Return to top Browse Antibody Plasmids The following plasmids...plasmid-based antibodies. ID Plasmid Description Return to top...

... capacity. Gamma-retroviral Vector Guide | Gamma-retroviral Plasmids Gamma-retroviral vectors, often referred... referred to as just retroviral vectors, have a relatively high transduction efficiency and only infect...genome, enabling long-term transgene expression. Retroviral vectors are popular for making stable cell lines...80–130 nm Dividing/Non-dividing Yes Low Gamma-retroviral Stable RNA < 8 kb 80–130 nm Dividing Yes Moderate...

...81070 rAAV2-retro helper AAV retrograde AAV packaging plasmid, expressing Rep2 and retrograde capsid Alla...eligibility, and more. ID Plasmid Description PI Return to top AAV Packaging Plasmids Packaging plasmids...name, capsid, and more. ID Plasmid Gene/Insert PI Return to top AAV Helper Plasmids Wild-type AAV viruses...AAV vector production. ID Plasmid Description PI Return to top Other Resources Addgene's Viral Vector Packaging...

...Tth DNA Ligase 165574 pOpen-HSlig3 DNA Ligase III Return to top DNA Polymerases Addgene ID Plasmid Gene/...Polymerase 165573 pOpen-phi29pol phi29 DNA Polymerase Return to top Restriction Endonucleases Addgene ID Plasmid...165567 pOpen-XbaIR XbaI 165568 pOpen-NcoIR NcoI Return to top Reverse Transcriptases Addgene ID Plasmid...Thermus thermophilus (Tth) Reverse Transcriptase Return to top RNA Polymerases Addgene ID Plasmid Gene/...Polymerase 165576 pOpen-T3gene1 T3 RNA Polymerase Return to top Other Enzymes Addgene ID Plasmid Gene/insert...165555 pOpen-ALPI CIP (calf intestinal phosphatase) Return to top Additional Resources Bacterial Expression...

...Publication Return to top Bacteria ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to ...Publication Return to top Plant ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top...Insert Promoter Selectable Marker PI Publication Return to top CRISPR Resources Addgene has a large selection...