...expressing mCherry and firefly luciferase. pLentiEGFPdestablized - EFS-EGFPd2PEST-2A-MCS-Hygro - Lentiviral...

...Guide Empty-Backbone Resource Image is released to public domain by author. Source: Wikipedia, accessed November...

...Sylvestre Marillonnet 95 plasmids for cloning and assembling standardized parts to create eukaryotic multigene...

...uniqueness of CRISPRi, as compared to several recently published works on applying the wild-type CRISPR system ...

...advantage of AAV vectors is that there are well-established protocols for their production and purification...

...Lentivectors Toolbox Tyler Jacks Lab Protocols Publications Short hairpin RNA (shRNA): design, delivery,...

...viruses also integrate stably into the host genome, enabling long term transgene expression. There are some...

...Experiment Web References PAM Sequences Glossary Publications CRISPR Overview Bacteria have an interesting...to check the library information and original publication to see if a library is suitable for your experiment...specified by the gRNA plus the genomic PAM sequence Publications (by topic) CRISPR basics Anders, C., Niewoehner.... D., Gross, C. A., & Rosenberg, O. S. (2019). Enabling genetic analysis of diverse bacteria with Mobile-CRISPRi...

... of Cas9 in vivo and ex vivo , the Zhang Lab established Cre-dependent and constitutively expressing Cas9...

...would no longer be limited to small transgenes, enabling the development of further AAV applications. Return...South Carolina's Viral Vector Core AAV Overview Publications Viral serotypes and pseudotyping/gene therapy...

...Manipulation Select Expression System Design gRNA Publications Get Started CRISPR is a powerful system that...post CRISPR 101: Validating your Genome Edit . Publications Cong, L., & Zhang, F. (2015). Genome engineering...

...signal and emission in the near-infrared range, enabling enhanced bioluminescence imaging in vivo . Luciferases...

...short peptide linkers. Diabodies can be used for assembling protein nanostructures. Figure 2: An antibody...specific to your antibody and assay may already be published. If it is not, you’ll need to validate the antibody...

... vectors with various promoters, plasmids for assembling multiple gRNAs into one vector, and plasmids ...

...System? The laboratory of Bert Vogelstein has published their method for generation of recombinant adenoviruses...

...relevant to the phenotype being studied. Examples of published selection mechanisms for lentiviral CRISPR libraries...

... is important to note that LIC has difficulty assembling DNA fragments with repetitive sequences and DNA...

... Lentiviral Production & Titration Protocols Publications Retroviral integration site selection. Desfarges...

...local concentration near the cell surface, and enabling the ion channel to function. TULIPs Tunable light-inducible...

...which contains several restriction enzyme sites, enabling easy insertion of DNA by restriction enzymes digestion...