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Showing: 541 - 560 of 576 results

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Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods

Type

Blog Post

...assembly a ligase is added to repair the nicks in vitro, whereas in SLIC these constructs are repaired in...
Treating Muscular Dystrophy with CRISPR Gene Editing

Type

Blog Post

...effects, each group finds very low to no off-target activity at the ten highest-ranking predicted off-target...
How to Negotiate a Successful Lab Start Up Budget

Type

Blog Post

...opinions on creating and negotiating budgets and invite you to leave a comment below Happy Sciencing, ...
Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

Type

Blog Post

...proteins and confirmed that there was no cross-reactivity, opening the door for immunostaining analysis...
CRISPR 101: Off-Target Effects

Type

Blog Post

...by Cas does not always correlate with catalytic activity at those sites, especially when DNA mismatches...
Antibodies 101: Multiplex Immunofluorescence

Type

Blog Post

...handling steps, or there’s some unexpected cross-reactivity. Keep in mind that you may need to optimize the...
Recombinase-based State Machines Enable Order-dependent Logic in vivo

Type

Blog Post

...regulation, as long as they can control recombinase activity. For example, post-transcriptional recombinase...
PiggyBac-ing Through the Genome Editing Field

Type

Blog Post

...repeats (ITRs) found on the piggyBac™ donor for its activity. In summary, the piggyBac™ transposon is a powerful...
Plasmids 101: Restriction Cloning

Type

Blog Post

...stains): Stain Pre or Post Run? Visualization Sensitivity (ng DNA) SYBR Safe Pre and Post Blue or UV ...
Finding Your Perfect Job After University

Type

Blog Post

...have meetings between teams. This communication is vital because otherwise I’d lose touch with my colleagues...
Building Global Connections with the International Mentorship Program USA-EUROPE

Type

Blog Post

... metastasis as well as teaching and mentoring activities. Follow her on twitter @zcastan or on linkedln...
A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression

Type

Blog Post

...expressing TH protein indicating that there is promoter activity. Additionally, some neurons express low levels...
A Guide to Getting Started in Undergrad Research

Type

Blog Post

...When you are hunting for labs to join, one of the vital things to look for is a supportive environment—it...
Important Considerations When Using AAVs

Type

Blog Post

...virus serotypes: impact of larger genomes on infectivity and postentry steps." Journal of virology 79.15...
Lambda Red: A Homologous Recombination-based Technique for Genetic Engineering

Type

Blog Post

...particularly interested in science communication and in vitro diagnostics. References 1. Costantino N, Court...
Antibodies 101: Designing Your First Flow Panel

Type

Blog Post

...lab. Let's say I am culturing HEK 293T cells in vitro. I transduced the cultured cells with a virus that...
Antibodies 101: Introduction to Antibodies

Type

Blog Post

...sequences can be optimized for specificity and sensitivity, for example by changing certain amino acids ...
Typing CRISPR Systems

Type

Blog Post

...called CasPEDIA, with information about enzyme activity, experimental conditions, and more. Type II Type...
With an Eye Towards the Future, We Look Back at the March for Science

Type

Blog Post

... clinic regulations, evidence-based research is vital in combating misinformation and propaganda that ...
CRISPR 101: Multiplex Expression of gRNAs

Type

Blog Post

...knockout or edit. This can help reduce off-target activity. Deleting a large region of the genome by removing...

Showing: 541 - 560 of 576 results

Results per page:

Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods

Treating Muscular Dystrophy with CRISPR Gene Editing

How to Negotiate a Successful Lab Start Up Budget

Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

CRISPR 101: Off-Target Effects

Antibodies 101: Multiplex Immunofluorescence

Recombinase-based State Machines Enable Order-dependent Logic in vivo

PiggyBac-ing Through the Genome Editing Field

Plasmids 101: Restriction Cloning

Finding Your Perfect Job After University

Building Global Connections with the International Mentorship Program USA-EUROPE

A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression

A Guide to Getting Started in Undergrad Research

Important Considerations When Using AAVs

Lambda Red: A Homologous Recombination-based Technique for Genetic Engineering

Antibodies 101: Designing Your First Flow Panel

Antibodies 101: Introduction to Antibodies

Typing CRISPR Systems

With an Eye Towards the Future, We Look Back at the March for Science

CRISPR 101: Multiplex Expression of gRNAs

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