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We narrowed to 620 results for: PROC

Showing: 561 - 580 of 620 results

  1. Antibody Guide

    Type
    Guide
    ...randomized reorganization process, creating a unique binding site. This process, known as V(D)J recombination...delaying a process through sequential rounds of validation. Validation of an antibody is a process separate...binding partners, location in the cell, and sample processing can hide or reveal epitopes on an antigen. Monoclonal...can interfere with the conjugate or conjugation process. Sodium azide, an antimicrobial agent, can block...proteins to detect simultaneously. Conjugation The process of attaching signaling molecules to an antibody...available. Tissue samples may need additional processing steps, such as sonication. Denature proteins,...proteins from a complex mix. Overview of application Process the tissue or cell culture samples to extract proteins...

  2. Molecular Biology Reference

    Type
    Guide
    ...The actual process of Illumina NGS is not that different from Sanger sequencing. This process, like Sanger... similarly referred to as cloned DNA, and this process of generating multiple identical copies of a recombinant...molecule is known as DNA or molecular cloning . The process of molecular cloning enabled scientists to break... synthesized RNA signals for the transcription process to stop. An expression vector can also include ...Sequencing In 1975, Frederick Sanger developed the process termed Sanger sequencing, sometimes referred to...sequence of the complementary strand. The replication process can also be done in a test tube to copy DNA regions...adding nucleotides. The major difference in this process occurs when the polymerase incorporates a fluorescently...

  3. Lentiviral Vector Guide

    Type
    Guide
    ...lentiviral vectors from your plasmids (Figure 5). This process requires a production cell line, typically human...with or without determining the viral titer. This process of viral production followed by transduction is...can be altered by changing the envelope gene, a process called pseudotyping. VSV-G is by far the most common...genes may be involved in different biological processes by surveying a pool of genetic mutants for various...definition, at the beginning of R, is capped, and proceeds through U5 and the rest of the provirus. Third-generation...plasmids, packaging cell line). Infection The natural process of entry and multiplication of a pathogen, like...and genes provided in trans . Transduction The process of artificially introducing foreign DNA into eukaryotic...

  4. Adeno-associated virus (AAV) Guide

    Type
    Guide
    ...genome. Instead, the linear rAAV DNA is typically processed into a double-stranded circular episome which ...AAV serotypes. Pseudotypes Pseudotyping is the process of producing viral vectors in combination with ... proteins that carry out a naturally occurring process known as protein trans-splicing. As these methods...plasmids, packaging cell line). Infection The natural process of entry and multiplication of a pathogen, like... or replicating. Lytic cycle Viral replication process where the virus uses the cell machinery to produce... the main cell body, or soma. Transduction The process of artificially introducing foreign DNA into eukaryotic...eukaryotic cells using viral vectors. Transfection The process of artificially introducing foreign DNA into eukaryotic...

  5. Gamma-Retroviral Vector Guide

    Type
    Guide
    ...can be altered by changing the envelope gene, a process called pseudotyping. VSV-G is by far the most common...to their integration into a host’s genome. The process of generating stable cell lines with gamma-retroviral...specificity of infection for CD4-expressing cells. Proceedings of the National Academy of Sciences , 94 (16)...the beginning of R, has a 5' cap structure, and proceeds through U5 and the rest of the provirus. Some ...plasmids, packaging cell line). Infection The natural process of entry and multiplication of a pathogen, like...and genes provided in trans . Transduction The process of artificially introducing foreign DNA into eukaryotic... cells using viral methods. Transformation The process where bacteria take up DNA from their environment...

  6. Adenovirus Guide

    Type
    Guide
    ...pre-existing immunity. Pseudotypes Pseudotyping is the process of producing viral vectors in combination with ...system for generating recombinant adenoviruses . Proceedings of the National Academy of Sciences of the United...plasmids, packaging cell line). Infection The natural process of entry and multiplication of a pathogen, like...agent at a specific time point. Transduction The process of artificially introducing foreign DNA into eukaryotic...eukaryotic cells using viral methods. Transfection The process of artificially introducing foreign DNA into eukaryotic...cells using non-viral methods. Transformation The process where bacteria take up DNA from their environment...

  7. Optogenetics Guide

    Type
    Guide
    ...frequently neurons) and their governing biomolecular processes. The tools and technologies developed for optogenetics...functions. Example schematic of an optogenetics procedure. A channelrhodopsin, fused to mCherry, is expressed...outside of the brain, rendering the experimental procedure less invasive. Different activation wavelengths...2013. Optobiology: optical control of biological processes via protein engineering. Biochem Soc Trans. 41... excitation/inhibition balance in information processing and social dysfunction. Nature. 477(7363):171...

  8. Cloning

    Type
    Guide
    ...the creation of recombinant DNA is an essential process used in scientific research and discovery. With...recombination reaction, drastically simplifying the process and reducing the amount of time required for cloning...nicks will be repaired by the normal replication process. It is important to note that LIC has difficulty...

  9. Plan Your Experiment

    Type
    Guide
    ...is necessary to model your specific disease or process of interest. Do you want to: Generate complete ... method of delivery and the cell type. Before proceeding with your experiment, it may be necessary to ...

  10. CRISPR Guide

    Type
    Guide
    ...condensed multiplexing capabilities , as it can process multiple gRNAs under a single promoter, and can...which include Cas13a , Cas13b , and Cas13d , can process crRNA precursors to mature crRNAs. Upon ssRNA recognition...

  11. Science Guides

    Type
    Guide
    ...frequently neurons) and their governing biomolecular processes. The tools and technologies developed for optogenetics...

  12. Addgene's eBook Collection

    Type
    Guide
    ...transferable skills, and tips on the job application process. Close About Overlay Select CRISPR 101 en español...

  13. Guide to Using Pooled Libraries

    Type
    Guide
    ...are the general steps to take in the screening process. Amplify the library (electroporation and maxiprep...

  14. Promoters

    Type
    Guide
    ...recognition and binding. On the other hand, the process in eukaryotes is much more complex. Eukaryotes ...

  15. Video Library

    Type
    Protocol
    ...ordering process Ordering Page MTA FAQs Answers to frequently asked questions about the MTA process Help ...content, including how-to screen captures, lab procedure protocols, and career advice videos. Educational...Video protocol guides for standard laboratory procedures. How-To Videos Screencapture walk-throughs to...Gel electrophoresis is the standard laboratory procedure for separating DNA by size for visualization and...Page MTA Guide - Requests Your guide to the MTA process when requesting samples from Addgene Technology...

  16. AAV Production in HEK293 Cells

    Type
    Protocol
    .... Thaw a new vial of cells after 30 passages. Procedure Trypsinize and resuspend the HEK293T cells from...850 mL. Return to the incubator for 24 h–36 h. Proceed with transfection: Calculate the amount of each...transfer supernatant to a sterile 500 mL bottle. Process the supernatant as follows: Filter through a 0.45...precipitation. Precipitation of the viruses can proceed overnight at 4 °C if needed. Transfer the entire.... Combine resuspended pellets and keep on ice. Process the cell pellet from above as follows: Resuspend...any residual DNA carried over from the packaging process. Incubate at 37 °C for 45 min. You are now ready...supernatant can be kept overnight at 4 °C before proceeding with the purification protocol. Sample Data Figure...

  17. CRISPR Library Amplification

    Type
    Protocol
    ...Libraries Molecular Biology Reference How do I process my Addgene pooled library? Introduction Please ...to autoclaved, sterile reagents for all steps. Procedure Day 1 Add 200 ng DNA to each 50 µL aliquot of ...not to split or gouge agar during the scraping process. Add each scrape into a 50 mL conical tube on ice...reagents (not including the recovery media!). Do not proceed with Maxipreps or NGS until adequate transformation...Visit our Help Center to get details on how to process your Addgene pooled library...

  18. Protocol - Bacterial Transformation

    Type
    Protocol
    ...DNA Ligation Introduction Transformation is the process by which foreign DNA is introduced into a cell.... competent cells. This is a relatively simple procedure and is useful for performing low efficiency transformations...media Competent cells DNA you'd like to transform Procedure Take competent cells out of -80°C and thaw on ...competent cells) to ensure that your transformation procedure is working. TIP: Sometimes less is more. Although...

  19. Affinity Purification of Recombinant Antibodies with Protein A or Protein G

    Type
    Protocol
    ... following your institution’s hazardous waste procedures. Last Update: February 7, 2022 Protocol Video... filter. Aliquot and freeze upright at -20 °C Procedure Section 1: Affinity chromatography Equilibrate...concentration of the pooled sample is above 1.0 mg/mL proceed to Option 1 with a buffer exchange using a Zeba...concentration of the pooled sample is below 1.0 mg/mL proceed to Option 2 with a buffer exchange/concentration...

  20. Centrifugation

    Type
    Protocol
    ...densities is essential to many different experimental procedures, such as minipreps and RNA extractions. Plus,...temperatures. This protocol will cover the general procedure and features to keep in mind when using any centrifuge...the time and speed needed for your experiment. Procedure An example of a balanced tabletop centrifuge, ...
Showing: 561 - 580 of 620 results