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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 581 results for: SUB

Showing: 561 - 580 of 581 results

  1. Antibodies 101: Validation

    Type
    Blog Post
    ...antibody that you want to use to look at the subcellular localization of a protein of interest. Conveniently...

  7. Deep Dive: qPCR

    Type
    Blog Post
    ...lines and/or tissue types.  Please do note my oh-so-subtle use of reference genes, plural. Your data will ...

  8. Antibody Guide

    Type
    Guide
    ...antibodies can be isotype-specific or even isotype subclass-specific. This specificity can be used to ensure...Isotype Description Configuration and Valency Subclasses IgA Prevalent antibody in secretions (e.g. tears...should never be stored below 4 °C. Certain isotype subclasses, such as IgG3, aggregate after thawing and therefore...reaction in the presence of several different substrates. The luminescence produced by the reaction can...different species of origin, isotypes, and isotype subclasses of a primary antibody, which becomes the limiting...and block the membrane. Activate the HRP with a substrate kit. Image the membrane using either X-ray film... cytometry requires a negative control, a small subset of the sample with no antibodies. It also requires...

  9. Optogenetics Guide

    Type
    Guide
    ...below, can be targeted and expressed in specific subsets of neurons, allowing precise spatiotemporal control...other species include: CsChR (from Chloromonas subdivisa ), CoChR (from Chloromonas oogama ), and SdChR...blue-shifted channelrhodopsin from Platymonas subcordiformis 445 CoChR Channelrhodopsin from Chloromonas...oogama CsChR Channelrhodopsin from Chloromonas subdivisa CheRiff Channelrhodopsin from Scherffelia dubia...in all GABAergic neurons. In this case, the subpopulation of GABAergic neurons being activated by the ...express an opsin ONLY in a genetically defined subpopulation of cells within the injection site of the viral...

  10. Guide to Using Pooled Libraries

    Type
    Guide
    ... be small, if they are designed to cover only a subset of genes, or very large (e.g. the Toronto KnockOut... be used to make virus. This pooled virus is subsequently used to deliver the plasmids to the cells of...mechanism. You’ll infect two sets of cells and subject one set to selection while the other serves as ...

  11. Chemogenetics Guide

    Type
    Guide
    ...light-sensing optogenetic element. When the luciferase substrate (luciferin) is added, the luciferase enzyme generates...on the type of luciferase used, the luciferin substrate may be the native coelenterazine (CTZ) or chemical...targeted to specific tissues, cell types or even subcellular regions of a neuron. Cell-specific expression...

  12. CRISPR Guide

    Type
    Guide
    ...to uridine, which is read as thymidine during subsequent DNA repair, creating a C to T change. Modern ...sequence will remain unchanged and available as a substrate for another round of prime editing. Figure 6: ...Recombinases and Integrases Recombinases, and their subfamily integrases, are used for large scale edits. These...Whatever the chosen epitope tag, the locus is subsequently isolated by affinity purification. After purification...

  13. Modular Cloning Guide

    Type
    Guide
    ... multi-subunit protein complexes in various hosts. Expression cassettes for individual subunits are first...

  14. Gamma-Retroviral Vector Guide

    Type
    Guide
    ...Plasmid Elements Glossary Gamma-retroviruses are a subtype of retrovirus and belong to the Retroviridae family.... Immunogenicity The ability of a molecule or substance to induce an immune response in the body. in cis...

  15. Adenovirus Guide

    Type
    Guide
    ...DNA removed from the viral genome. Over time, subsequent generations of rAdV vectors have been developed.... Immunogenicity The ability of a molecule or substance to induce an immune response in the body. in cis...

  16. Adeno-associated virus (AAV) Guide

    Type
    Guide
    ...homologous recombination, when both sequences present substantial sequence overlap. Co-expression induces homologous.... Immunogenicity The ability of a molecule or substance to induce an immune response in the body. in cis...

  17. Educational Resources

    Type
    Guide
    ...Guides Browse our in-depth instructional content on subjects such as: CRISPR Chemogenetics Optogenetics Genetic...

  18. Promoters

    Type
    Guide
    ...TATA-binding protein binds the TATA box and helps in the subsequent binding of the RNA polymerase. A transcription...

  19. Cloning

    Type
    Guide
    ...restriction enzyme sites. You can easily move (subclone) any piece of DNA that already has restriction...

  20. Plan Your Experiment

    Type
    Guide
    ...variety of modifications and will likely contain a subset of wild type cells. If you are screening a variety...
Showing: 561 - 580 of 581 results