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We narrowed to 949 results for: app

Showing: 61 - 90 of 949 results
  1. A Practical Approach to Choosing the B(right)est Fluorescent Protein

    Type
    Blog Post
    ...protein for your application. Selecting the best fluorescent protein for a specific application can seem like...under conditions that best mimic the intended application. The three key properties for live cell imaging...theoretical brightness. The theoretical brightness often appears in tables with fluorescent protein properties and...experimental set up. The practical brightness takes all application specific parameters into account, including the... picture of what can be expected in a 'real' application than the theoretical brightness. Consequently...brightness in a system closely mimicking the future application. In figure 2 we provide an example of the comparison...
  2. Performing In Vivo CRISPR Screens Using the FITS Approach

    Type
    Blog Post
    ...The Framework for In vivo T cell Screens (FITS) approach (Milling and Markson et al., 2024), developed ...compatible with single gene KO and pooled screening approaches; (4) encodes a Thy1.1 selectable marker to facilitate... et al., 2024.   Proof of concept The FITS approach was used to optimize conditions for a screen that...
  3. Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards

    Type
    Blog Post
    ...cas9 gene and single guide RNAs (sgRNAs) with appropriate flanking homology into a germline cell can create...RNA-guided gene drive. In principle, this event could happen even if there isn't any homology available. Consequently...local biosafety officer should be familiar with appropriate measures. Barriers should be a component of all...eventual release should consider building an appropriate immunizing reversal drive at the same time to...species in question), why not use them whenever applicable?Against this, consider the cost of an accidental...An accidental release could delay real-world applications against scourges such as malaria and dengue .... Disclaimer: Recommendations concerning the appropriate degree of confinement are those of the author...
  4. Virtual Science Conference Coming Up? Three Addgenies Share Their Experience

    Type
    Blog Post
    ... the app. Technical difficulties? Attending a conference virtually means getting to know the app or virtual...conferences with multiple sessions happening at once, virtual conferences may have apps or interactive calendars...was able to get in touch with the author. “In the app, you can write a message to the author of the poster...there may be a “place” for networking within the app. For example, the NeuroSymposium had virtual tables... puts butterflies in your stomach, the virtual approach might be less stressful. For both conferences,...Having an on-site conference canceled may be disappointing, but there are many creative ways to adapt and...
  5. CRISPR Plasmids - dCas9-FokI

    Type
    Collection
    ...RNA Targeting RNA Targeting RNA Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled...
  6. Proximity Labeling: A Powerful Tool for Protein Complex Purification and Proteomic Mapping

    Type
    Blog Post
    ...There are approximately 2-4 million proteins per cubic micron in bacteria, yeast, and mammalian cells...based affinity purification and mass spectrometry approaches. Recently however, scientists have developed ...miniturbo Split-BioID: A conditional proteomics approach to monitor protein-protein interactions Split-... PMC3308701. Rhee, Hyun-Woo, et al. "Proteomic mapping of mitochondria in living cells via spatially restricted..., et al. "Split-BioID a conditional proteomics approach to monitor the composition of spatiotemporally...proximity-based labeling methods for interactome mapping. F1000Research, 8.  ...
  7. DNA Service - Cloning Grade DNA

    Type
    Collection
    ...Cloning grade DNA is designed for use in cloning applications. Direct transfection into cells is not recommended...
  8. Plasmid Collections

    Type
    Collection
    ...resources group plasmids together based on type or application. Browse our guides to find the plasmids and science...
  9. CRISPR Plasmids - Cascade-Cas3

    Type
    Collection
    ..., some CRISPR-Cas3 plasmids take an all-in-one approach and include Cas3 and Cascade on one single plasmid...
  10. CRISPR Plasmids and Resources

    Type
    Collection
    ...fused to deaminases can edit bases in RNA. Other Applications Screen CRISPR libraries are a powerful tool ...
  11. CRISPR Plasmids - Base Edit

    Type
    Collection
    ...and are thus well suited to directed evolution applications. Examples of these base editing systems include...
  12. Viral Vector Guides and Plasmids

    Type
    Collection
    ...strong immunogenic response, useful for clinical applications as vaccines. Selecting a Viral Vector Type Each...
  13. mTOR Pathway

    Type
    Collection
    ...pro-proliferative and anti-apoptotic signals. mTORC2, which appears to signal upstream of Akt, also promotes cell survival...
  14. Chemogenetics AAV Preps

    Type
    Collection
    ...Bryan Roth 65417 pAAV-hSyn-dF-HA-KORD-IRES-mCitrine kappa-opioid receptor (KOR) - Inhibition IRES mCitrine...
  15. Open Enzyme Collection

    Type
    Collection
    ...enzymes. These enzymes are useful in both basic and applied fields as diverse as genomics, diagnostics, biodiversity...
  16. Plant Plasmids and Resources

    Type
    Collection
    ...window) — Tools for gene and protein expression and mapping of gene expression in plants. Grassius (Link opens...
  17. TALEN Plasmids and Kits

    Type
    Collection
    ...Golden Gate TALEN 2.0 48705 pTAL7a Boris Greber Application of TALEN technology in hPSCs pTAL7a and pTAL7b...
  18. CRISPR Plasmids - Tagging

    Type
    Collection
    ...RNA Targeting RNA Targeting RNA Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled...
  19. Brain Armamentarium

    Type
    Collection
    .... Useful for nuclear isolation and scRNA-seq applications. Jonathan Ting Viviana Gradinaru 163505-PHPeB...
  20. Caltech Systemic Capsids

    Type
    Collection
    ....PHP.N with GRE or transgenic animal For PNS applications in rodents and NHPs: Broad tropism - AAV-MaCPNS2...
  21. Early Career Researcher Toolbox: Free Online Molecular Biology Tools

    Type
    Blog Post
    ...for your needs 5 great apps for lab life  Sharing your lab protocols using apps to save you time and track...sign-in required.)  DailyCalcs Science Calculator app for iPhone Reagent and Data Repositories These links...paste into other applications Primer design tools Many of the above-listed plasmid mapping tools have built-in...Primer design. Plasmid mapping. DNA sequence analysis. We all have our favorite tools for tackling these...molecular biology tools all in one place. Plasmid mapping These tools are for viewing, editing or making ...the paid version provides more tools for plasmid mapping and design (Figure 1). Benchling: While you might... desktop-based software for plasmid design and mapping. ApE (A plasmid Editor): A free, donation-based...
  22. Trimmer Lab NeuroMab Collection

    Type
    Collection
    ...extensively validated for neuroscience research applications. A substantial number of these have been cloned...antibodies are produced in-house and undergo application-specific validation and quality control by Addgene...
  23. Hot Plasmids: Winter 2025

    Type
    Blog Post
    ...neurodegeneration research: Anti-MAPT [AT8] and Anti-APP [6E10-A5]. Both are recombinant mouse monoclonal ...Figure 5: Immunohistochemistry with Addgene's Anti-APP [6E10-A5] recombinant antibody in human postmortem... antibodies here! Wu, Z., Wang, W. (2024). Anti-APP [6E10-A5] in Immunohistochemistry (Human). Addgene...in human cells, has been uniquely challenging to apply in dicotyledonous plants, or dicots. But Jae-Yean...efficiency barrier in tomatoes" by combining several approaches (Vu et al., 2024). Their optimized strategy uses... wild type (WT), two weeks after herbicide was applied to both plants. Adapted from Vu et al., 2024 bioRxiv...CC-BY-NC-ND 4.0 International license. With this approach, the team was able to drive prime editing in both...
Showing: 61 - 90 of 949 results