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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 644 results for: ATO

Showing: 41 - 60 of 644 results

  1. Deisseroth INTRSECT Collection

    Type
    Collection
    ...fluorophores, excitatory and inhibitory opsins , genetically-encoded calcium indicators, and rabies targeting...INTRSECT (intronic recombinase sites enabling combinatorial targeting) is a synthetic molecular targeting...doubly-specified combination of genetic and/or anatomical-defined parameters, by placing two orthogonal...Flp AND NOT Cre Yes Dual recombinase-dependent: Excitatory Opsins Addgene ID Plasmid Logic Sites and Mutations...window) Cummings KA, Clem RL. 2020. Prefrontal somatostatin interneurons encode fear memory. Nat Neurosci...

  2. Synthetic Biology - Overview

    Type
    Collection
    ...and synthetic regulatory elements, including promoters, terminators, repressors, activators, and more. Also... Lab Repressors , Terminators , Light Signaling , Sigma Factors , Resource Allocator and Orthogonal Switches...

  3. Luciferase Plasmid Collection

    Type
    Collection
    ...made luciferase a popular tool for scientists. Regulatory elements such as promoters, enhancers and untranslated...backbone plasmids into which you can clone your regulatory element or gene of interest into to create a ...Lentiviral expression of firefly luciferase and dTomato. A gene of interest can also be inserted into the...Reporter Constructs Already know what gene or regulatory element that you need a luciferase reporter for...search box to find a construct that contains your regulatory element of interest. ID Plasmid Description Gene...Optimized STARR-seq (Self-Transcribing Active Regulatory Region) : Genome-wide screening of enhancers ...

  4. p53 Pathway

    Type
    Collection
    ..., which disrupt its interaction with negative regulators, increase its stability and DNA binding activity...activity, and allow it to bind transcriptional co-activators and modulate transcription. The list of p53 transcriptional...proto-oncogene, E3 ubiquitin protein ligase MDMX p53 regulator; also known as MDM4 Noxa Phorbol-12-myristate-...peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1 PERP TP53 apoptosis...binding component 3 Reprimo TP53 dependent G2 arrest mediator candidate Scotin Shisa family member 5 Sestrins...

  5. Fluorescent Protein Guide: Empty Backbones

    Type
    Collection
    ... pFA6a-link-tdTomato-SpHis5 - Yeast Expression tdTomato-N1 - Mammalian Expression tdTomato-C1 - Mammalian... Bacterial Expression tdTomato 554 581 95 4.7 1 hr Tandem-dimer pCSCMV:tdTomato - Mammalian Expression...Mammalian Expression tdTomato-pBAD - Bacterial Expression Jump to Top Red Protein Excitation (nm) Emission...

  6. NETRF

    Type
    Collection
    ...from the NETRF-funded investigators listed below. Depositing Principal Investigators (PIs) PI Institution...Shivdasani Dana-Farber Cancer Institute Epigenetic Regulators of Intestinal Endocrine Cells and Carcinoid Tumors...Tumors Qiao Zhou Harvard University Epigenetic Regulators of Intestinal Endocrine Cells and Carcinoid Tumors...

  7. CRISPR Plasmids - Activate Gene Expression

    Type
    Collection
    ...gRNA sequence to direct the dCas9-activator to promoter or regulatory regions of your gene of interest....Catalytically dead dCas9 fused to a transcriptional activator peptide can increase transcription of a specific...separate gRNA expression plasmid to target the dCas9-activator to your specific locus. Want more information ...

  8. Zhang Lab CRISPR Page

    Type
    Collection
    ...Expresses dCAS9-VP64 activator with 2A GFP 61423 : Expresses the MS2-P65-HSF1 activator helper complex with...screening library CRISPR/Cas9 Synergistic Activation Mediator (SAM) is an engineered protein complex for the...: lentiviral vector encoding the MS2-P65-HSF1 activator helper complex with a 2A Hygro resistance marker...JS, Konermann S, Trevino AE, Scott DA, Inoue A, Matoba S, Zhang Y, Zhang F. Cell . 2013 Sep 12;154(6):...

  9. CRISPR Plasmids - C. elegans

    Type
    Collection
    ...gRNA sequence to direct the dCas9-activator to promoter or regulatory regions of your gene of interest....Catalytically dead dCas9 fused to a transcriptional activator peptide can increase transcription of a specific...separate gRNA expression plasmid to target the dCas9-activator to your specific locus. ID Plasmid Gene/Insert...

  10. CRISPR Pooled gRNA Libraries

    Type
    Collection
    ...3rd 10 187,535 Advanced Catalogue of Epigenetic Regulators (ACER) 226117 Knockout Human Lu 3rd 10 8,205 ...Knockout Mouse Tolar 3rd ~4 78,637 Mouse Chromatin Regulator Library 200011 Knockout Mouse Griffin, Manguso...4 17,032 arrays Mouse Cardiac Transcriptional Regulators CRISPR Knockout Library 138015 Knockout Mouse...CRASP-Seq Gene KO Library 232069 Knockout Human Gonatopoulos-Pournatzis 3rd Varies 95,893 CRASP-Seq BE Tiling...Tiling Library 232070 Base Editing Human Gonatopoulos-Pournatzis 3rd Varies 8,594 CROPseq-iT7 Human CRISPR...

  11. CRISPR Plasmids - Drosophila

    Type
    Collection
    ...gRNA sequence to direct the dCas9-activator to promoter or regulatory regions of your gene of interest....Catalytically dead dCas9 fused to a transcriptional activator peptide can increase transcription of a specific...separate gRNA expression plasmid to target the dCas9-activator to your specific locus. ID Plasmid Gene/Insert...

  12. CRISPR Plasmids - Yeast

    Type
    Collection
    ...gRNA sequence to direct the dCas9-activator to promoter or regulatory regions of your gene of interest....Catalytically dead dCas9 fused to a transcriptional activator peptide can increase transcription of a specific...separate gRNA expression plasmid to target the dCas9-activator to your specific locus. ID Plasmid Gene/Insert...

  13. CRISPR Plasmids - Bacteria

    Type
    Collection
    ...gRNA sequence to direct the dCas9-activator to promoter or regulatory regions of your gene of interest....Catalytically dead dCas9 fused to a transcriptional activator peptide can increase transcription of a specific...separate gRNA expression plasmid to target the dCas9-activator to your specific locus. ID Plasmid Gene/Insert...

  14. Working with Nuclear Receptors

    Type
    Blog Post
    ... for a curated list of coregulators). Coregulators can function as coactivators and corepressors depending...complexes with secondary coregulator (or co-coregulator) partners. The coregulator ‘core’ complex is composed...feature afforded by coactivator complexes suggests that coactivators may be integrators of multiple cell ...illnesses, and the metabolic syndrome. Coregulators Coregulators constitute a group of some 400 molecules...that coregulators belong to distinct complexes may explain how more than 300 different coactivator proteins...manner. Because coregulators exist as multiprotein complexes, a member of a single coactivator complex can... W. (2002). Combinatorial control of gene expression by nuclear receptors and coregulators. Cell 108, ...

  15. CRISPR Plasmids - Mammalian Expression

    Type
    Collection
    ...gRNA sequence to direct the dCas9-activator to promoter or regulatory regions of your gene of interest....Catalytically dead dCas9 fused to a transcriptional activator peptide can increase transcription of a specific...separate gRNA expression plasmid to target the dCas9-activator to your specific locus. ID Plasmid Gene/Insert...

  16. CRISPR Plasmids - Plants

    Type
    Collection
    ...gRNA sequence to direct the dCas9-activator to promoter or regulatory regions of your gene of interest....Catalytically dead dCas9 fused to a transcriptional activator peptide can increase transcription of a specific...separate gRNA expression plasmid to target the dCas9-activator to your specific locus. ID Plasmid Gene/Insert...

  17. COVID-19 Resources

    Type
    Collection
    ...platforms to reliably detect nucleic acids at the atomolar level. For more information read Addgene's Blog...and open-access SARS-CoV-2 detection assay for laboratory and home testing. Kellner, et al. bioRxiv 2020.06.23.166397...Cathepsin L Functionally Cleaves the Severe Acute Respiratory Syndrome Coronavirus Class I Fusion Protein Upstream...TMPRSS2 augments entry driven by the severe acute respiratory syndrome coronavirus spike protein. . PCP4 - ...

  18. How-to: 5 Steps to a Great Panel Discussion

    Type
    Blog Post
    ...Choose a moderator with a backbone. It is nice to have a subject area expert, but the moderator is not ...LinkedIn profiles of all panelists to the moderator. The moderator and organizers should develop a list of...(lapel mics are best). The moderator takes control It is the moderator's job to prevent anyone from ...effortless to the audience, but the organizers and moderator should have a well-planned approach to making ... of 4-6 pre-event interview questions. The moderator (possibly with an organizer) should set up a 30 minute...to schedule. In the pre-event interviews, the moderator should be taking good notes and discovering interesting...opinions that each panelist can contribute. The moderator should identify the funny, provocative or interesting...

  19. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    ... the perfect voltage indicator and optogenetic actuator pair. Ideally, indicators should possess fast ...lab wished to improve on the Arch indicator and their collaborators in Robert Campbell’s lab carried out...kinetics. By combining both the voltage indicator, QuasAr, and actuator, CheRiff, in the bicistronic Optopatch...activation of specific genes using dCas9-VP64 activator fragments (dCas9(C)-FKBP-2xNLS-VP64 and dCas9(...62885 & 62886) Rapamycin-inducible dCas9-VP64 activator set (Addgene plasmids 62887 & 62888) Zetsche... which will ultimately co-localize the VP64 transactivator with a dCas9 that has been targeted to a very...tool for activating endogenous genes Moritoshi Sato's Lab has engineered CRISPR-Cas9 to create a photoactivatable...

  20. New Viral Vectors - Winter 2025

    Type
    Blog Post
    ...Roth New serotype pAAV-EF1α-F-FLEX-mNaChBac-T2A-tdTomato AAV8 Molecular tool Massimo Scanziani New viral...Optogenetics Jonathan Ting New viral prep pAAV_BiSSTe10_dTomato_nlsdTomato AAV-PHPeB Control Gordon Fishell New...Optogenetics Gordon Fishell New viral prep pAAV_BiCHATe27_dTomato_nlsdTomato AAV-PHPeB Control Gordon Fishell New...Optogenetics Gordon Fishell New viral prep pAAV_BiVIPe4_dTomato_nlsdTomato AAV-PHPeB Control Gordon Fishell New...Gordon Fishell New viral prep pAAV_BiLAMP5e3_dTomato_nlsdTomato_nlsdTomato AAV-PHPeB Control Gordon Fishell...Optogenetics Gordon Fishell New viral prep pAAV_BiPVe4_dTomato_nlsdTomato AAV-PHPeB Control Gordon Fishell New...Optogenetics Gordon Fishell New viral prep pAAV_BiPVe3_dTomato_nlsdTomato AAV-PHPeB Control Gordon Fishell New...
Showing: 41 - 60 of 644 results