..., and VP160 with 10. dCas9 activators and sgRNAs can be separately transfected (pmax dCas9 activators ...available as Gateway donor vectors [48218~48221] and can be transferred to Gateway destination vectors (e....opens in a new window) Article . Individual plasmids can be ordered via the links below: Table 1. dCas9 Activators...

... that can be used as empty backbones for your gene of interest for expression in plants. You can search... Transcription Factors The following collections can be used for studying gene expression in plants: Maize...luminescent proteins for expression in plants. You can search the table for features you are looking for...

...Within this set of lentiviral transfer vectors you can find reporter plasmids to test whether or not you've...this set are derived from pAdenoX from Clontech and can be used to generate adenovirus expressing a variety...reporters as well as the Cre and Flp recombinases. Genes can be cloned into these transfer vectors using shuttle...uniquely suited to simplify the cloning process as it can be used in blue-white screening and forms blue colonies...

...dsRNA can be delivered as shRNA (short hairpin RNA) or siRNA (short interfering RNA). Delivery can occur...similar mechanism. Since it is pre-processed, it can bypass the Dicer cleavage step and proceed to binding...therapeutic targets, and delivery strategies for cancer therapy . International Journal of Nanomedicine...

..., allowing for precise gene editing. These edits can include mutations seen in human patients, protein...scalable. Multiple gRNAs targeting multiple loci can be easily expressed in the same cell or organism....amenable to genetic screens. Pooled gRNA libraries can be used to identify genes that are important to a... Nickase mutant: cuts only one strand of DNA and can be used with paired gRNAs to lower off-target cleavage...fused to transcriptional activators or repressors can modulate transcription in a reversible manner. Epigenetics...Epigenetics : dCas9 fused to epigenetic modifiers can modulate transcriptions and create heritable epigenetic...labeled dCas9 or gRNAs that bind fluorescent proteins can be used to image genomic loci in live cells. RNA ...

...Cas9 viruses can be used to interrogate the genome in a variety of ways. Cas9 proteins can be guided to...plasmids that are currently in our repository. You can obtain the plasmid from us or receive a ready-to-... Gene Expression Catalytically-dead Cas9 (dCas9) can be fused to a transactivator and used as a transcriptional...

...the 5' end and AscI at the 3' end. These inserts can then be sub-cloned into a series of expression vectors...E9 A list of commonly-used bait and prey plasmids can be found here: Plasmid Type Plasmid Name Bait Cd4d3...: 10.3791/3881 (2012). Papers showing how AVEXIS can be used: Basigin is a receptor essential for erythrocyte...

...rtTA or TetR expression plasmid Stable cell lines can be made that continuously express a system component...binds with high affinity to tTA and rtTA; thus, dox can be used in both tetracycline on and off systems. ...induction profiles for other tetracycline derivatives can be viewed in Krueger et al, 2004. Some cell culture...may contain tetracycline or its derivatives which can result in unintended system induction. Investigate...expression vector, CMV promoter, and Blasticidin; can be used to make cell lines Tet-On Advanced; Plasmid...bacterial systems from E. coli presented the best candidates for inducible expression. Bacterial systems were...

...sequences are prone to misfolding and aggregation and can interfere with protein-protein interactions and nerve... nature of the disease, a small portion of cases can be attributed to known genetic factors such as defects...decision-making and language abilities. The disease can be classified as early-onset, where symptoms appear... of genetic modification and target species. You can also find our guide to using plasmid pooled libraries...protein, and more. Plasmids provided by the foundation can be found on our Michael J. Fox Foundation Plasmid...

...of synthetic biology is the ease with which parts can be combined to construct complex networks. This is... which ligates a promoter into an empty backbone can be repeated to ligate your gene of interest after...specific prefix and suffix sequences for each (these can be built into PCR primers when creating a new part...

...response induced by the viral capsid. Adenoviruses can be produced by several methods, the most common of... sequences from both plasmids. This DNA molecule can then be transfected into mammalian packaging cell... human adenovirus serotype 5 (HAdV5) genome that can be assembled in a one-tube reaction. ID Kit Description...

...distance between the donor and acceptor molecules and can therefore be used to calculate the distances between...intramolecular or unimolecular FRET). Fluorescent proteins can be used as donor and/or acceptor molecules in either...specific genes are also available. Custom biosensors can also be constructed using the cpFRET kit from the...Fluorescent Proteins for FRET The following plasmids can be used to create a fluorescent fusion protein with...

...spontaneously, although in rare cases Rett syndrome can be inherited. In females, random X inactivation results...mutant MECP2 . Skewing of these X inactivation ratios can affect disease severity. The MECP2 protein is a global...mutation, the location and type of MECP2 mutation can be a strong predictor of symptom severity. Broadly...Rett syndrome-specific differentiated cells that can be used to understand the mechanisms of the disease...line , an immortalized pre-neuronal cell line that can be differentiated into neurons Coriell Institute:...backbones. A few examples of additional CRISPR resources can be found below: Base Editing RNA Editing CRISPR-based...

...reduce unwanted off-target effects. Nickase mutants can also be used with a repair template to introduce ...dCas9 fused to a transcriptional activator peptide can increase transcription of a specific gene. Design...gRNA plasmids that do not co-express a Cas protein can be paired with a wide variety of Cas-containing plasmids...

...retroviruses called γ-retroviruses. γ-Retroviruses can package relatively high amounts of DNA (up to ∼8 ...Some disadvantages of γ-retroviruses are that they can only transduce dividing cells (this is because they...integrate randomly into the host's genome, which can lead to oncogenesis (termed insertional mutagenesis...

...CRISPR-Cas systems, are programmable endonucleases that can be used to perform targeted genome editing. RGNs ...More information about RGNs and CRISPR-Cas systems can also be found at http://www.crispr-cas.org . Users...

...sequence, they adopt an enzymatically active state and can bind and cleave additional RNAs regardless of homology...Type VI enzymes that function in mammalian cells can be used to attentuate RNA levels. In mammalian systems...

...HDR). HDR is much less error-prone than NHEJ and can be used to faithfully introduce specific genomic ...gRNA plasmids that do not co-express a Cas protein can be paired with a wide variety of Cas-containing plasmids...

...the genome defined by a guide RNA. The transposase can then integrate donor DNA. Editing by CASTs is not...components (TnsA, TnsB, TnsC, and TniQ). Type I CASTs can result in bidirectional insertions and may not be...

... Cre-lox can be used to turn shRNA constructs on or off. In floxed-shRNA constructs, Cre can excise the...Learn about the Cre-Lox system and how it can be used for genetic rearrangements and recombination. Use...expressing Cre at specific times or locations, you can precisely control expression of your gene of interest...recombination is a very useful tool, Cre expression can be toxic in some systems, notably Drosophila. Read...Cre expression construct for your experiment. You can search the table for the promoter, fusion, or expression...For more information on FLEx switch and what you can do with it read Addgene's Blog on FLEx Vectors . ...activates gene of interest by removing Stop sequences. Can be used for injection into fertilized zygotes Mammalian...