...remove genes flanked by either loxP or frt sites respectively, and empty vectors to express your gene of interest...

...Identification of proteins associated with an IFNgamma-responsive promoter by a retroviral expression system for...

...lab synthesized 110,139 unique DNA sequences corresponding to every previously observed instance of human...

...brain. The cause of this neuron death is unknown. Despite the idiopathic (having no specific known cause)...

...amplicons that cover your locus of interest and correspond to edited and non-edited cell populations. For...

... PubMed (Link opens in a new window) Tovote P, Esposito MS, Botta P, Chaudun F, Fadok JP, Markovic M, ...

...-NEmut control norepinephrine sensor (does not respond to NE) Norepinephrine sensor Li 20297 pAAV-EF1a-double...

...include GFP and puromycin as selectable markers, respectively, or constructs in which multiple sgRNAs may ...

...Mouse Weissman 3rd 5 10 107,415 214,830 DNA Damage Response MKOv4 Library 140219 Knockout Human J. Chen 3rd...

...both mouse and human cells in 2006 and 2007, respectively. To create iPSCs, fully-differentiated adult...

...Return to top Cell Junctions Cell junctions are responsible for forming connections and adhesions between...

...Yeast RPR1(TetO) yes, cut S. pyogenes URA3 Davis eSpCas9(1.1) 71814 Mammalian U6 yes, cut (enhanced) S. ...

...antigen to demonstrate the expected antibody dose response.... an antibody against the target to show a dose response. Sharing speeds science. We believe that sharing...curve . If you don’t see any color change or dose response, your antibody concentration may have been too...reaction is saturated and you don't see any dose response, your antibody concentration may have been too...

...The main property of primers is that they must correspond to sequences on the template molecule (must be...template strand). However, primers do not need to correspond to the template strand completely; it is essential...essential, however, that the 3’ end of the primer corresponds completely to the template DNA strand so elongation...

... TBK1 ALS Dominic Esposito 70626 R777-E342 Hs.TBK1-nostop TBK1 ALS Dominic Esposito 71433 pmGFP10C-­Tau...

...replication. 5’LTR 5’ long terminal repeat. RRE Rev response element. A.3 Related Products The following plasmids...Avoid stretches of 4 or more nucleotide repeats, especially repeated Ts because polyT is a termination signal...Optimal Puromycin Concentration Each cell line responds differently to puromycin selection. Addgene strongly...

...UV box (be sure to wear proper UV protection - especially for your eyes!), remove it from any gel tray ...bottom and sides with the razor blade. This is especially important during the DNA purification step, as...

...depicts the sequence as a series of colored peaks corresponding to one of the four nucleotide bases. This is...your sequencing company or core facility The corresponding primer sequence used for each reaction Tips ...

...sequence which must be built into the 5’ end of the respective primers. Below we use pNIC28-Bsa4 as an example... T4 Pol in the presence of dCTP (not dGTP), corresponding to the first base removed by the restriction...

...and ~25 kDa corresponding to the heavy chain (HC) and light chain (LC) proteins, respectively. There should...