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We narrowed to 1,005 results for: Eras

Showing: 41 - 60 of 1005 results

  1. Phage Directory: From Phage Therapy to a Repository of Phage Information

    Type
    Blog Post
    ...now, phage therapy is not routinely done in most parts of the world. In the US, phage therapy is used as...it comes to administering phage therapy. The laws behind phage therapy, acceptable endotoxin levels, etc..., 2017 Facilitating the search of phages for therapy Phage Directory started out as a directory of phage... only used phage in basic research but not therapeutically: not all labs in Phage Directory have the resources...information and potential for successful phage therapy, Sacher and Zheng also saw the need to archive ...

  3. An Interview with Connie Cepko - Gene Therapy, Plasmid Tools and Insights to Success

    Type
    Blog Post
    ...Targeting in Retinal Gene Therapies 10:17-14:34 : Progress in Treating Macular Degeneration and Other Retinopathies...Retinopathies 14:39-25:59 : Strategies to Make Gene Therapies More General (more than correcting a defect in a single...for Delivering Multiple Genes to Cells for Gene Therapy 28:40-35:10 : GFP Activatable Tools Being Developed... gene therapy...

  4. Virus Protocol - Generating Stable Cell Lines

    Type
    Protocol
    ... Protocols Generating Stable Cell Lines with Lentivirus Generating Stable Cell Lines with Lentivirus...Protocol to generate stable cell lines expressing a gene of interest from an integrated lentiviral vector...Service Introduction This protocol can be used to generate stable cell lines expressing a gene of interest...observed using transient transfection approaches, generating cell lines using lentiviral vectors enables long-term... in the lab by heating to 56 °C for 30 min. Considerations Before You Start The health of the target cell...polyclonal populations of resistant cells start proliferating and the individual wells become confluent, expand... over the culture. To overcome this, consider generating monoclonal lines from the early polyclonal populations...

  5. A New Generation of Adenine Base Editors Improves Editing in Primary Human Cells

    Type
    Blog Post
    ... eighth generation of adenine base editors, called ABE8 (Gaudelli et al., 2020). The generated ABE8s needed...important attribute for therapeutic application. ABE8s demonstrate an overall improved base editing capacity...primary human cells. Now, scientists at Beam Therapeutics have found a way to improve editing in primary...systems, ABE7.10 (and the starting point for a new generation of ABEs), consists of 3 components:  a deaminase... and Head of Gene Editing Technology at Beam Therapeutics, used a previously developed bacterial selection...showed comparable editing behavior to ABE8.x-d. Overall, ABE8s showed a median increase of 1.94x editing...research! Base editing in primary human cells  Cell therapy approaches can use base editors to correct mutations...

  7. Addgene-Seeding Labs Plasmid Grant: Accelerating Science Globally - 5 Scientists, 4 Countries & 3 Continents!

    Type
    Blog Post
    ...Rodrigo Arturo Rivera Solís, Universidad Autónoma de Yucatán, Mexico Dr. Rodrigo Arturo Rivera Solís, Associate... Accelerating science and supporting scientists globally are the shared mission of both Seeding Labs ...and to increase the potential for this drought tolerant plant, Dr. Robertson aims to make use of the CRISPR... improving expression of nutritionally and therapeutically significant proteins. In this project she aims...herbicides, such as glyphosate, in water. He aims to leverage a glycine oxidase reaction to indirectly detect...

  8. Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

    Type
    Blog Post
    ...protein-protein interactions that uses split fluorescent proteins tethered to potential protein interaction partners...been generated for yeast (Kim et al., 2019) and fruit flies (Bischof et al., 2018), the generation of such... answer — i.e., do two proteins interact or not? — FRET is preferable when the temporal dynamics of PPIs...Ahmed AW, Spinelli L, Brun C, Basler K, Merabet S (2018) Generation of a versatile BiFC ORFeome library for...used to detect the inter- and intramolecular interactions that underlie protein function. However, FRET...quantities (and the correct stoichiometric ratios) to generate useable data. What’s more, measuring and quantifying...method exists for visualizing protein-protein interactions (PPIs): Bimolecular Fluorescence Complementation...

  9. Starter guide to induced pluripotent stem cells (iPSCs) part 1:  A renaissance in regenerative medicine

    Type
    Blog Post
    ...models for developmental processes and promising therapeutic tools. The two unique characteristics that define...differentiate into specialized cell types [2]. We general speak of 4 levels of stem cell potency: Totipotency...They are isolated mostly from abnormal and/or supernumerary preimplantation embryos that are produced during... in many organs in the adult human body. These generate specialized cells for their location, mostly to... bone marrow only differentiate into cells of peripheral blood. Typically, there is a very small number...cells can be used for different research and therapeutic purposes. Both types have their advantages and...isolated from the human body Usage Universal. Can generate all adult cell types. Limited. Only specific cell...

  10. pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCs

    Type
    Blog Post
    ...possibilities for the clinical and therapeutic applications of iPSCs. The generation of human iPSCs (hiPSCs) now...programming spanning from fibroblasts and keratinocytes to peripheral blood mononuclear cells (PBMCs). PBMCs...LMO2-associated clonal T cell proliferation in two patients after gene therapy for SCID-X1. Science, 2003... Fukuda, Generation of induced pluripotent stem cells from a small amount of human peripheral blood using... to generate integration-free human-induced pluripotent stem cells from cord blood and peripheral blood...(Kushi) Mukherjee, a Postdoc at Massachussetts General Hospital. A little over a decade back when Yamanaka...Yamanaka”) factors, they changed the landscape of regenerative medicine. Their work opened up vast possibilities...

  11. A History of Genome Engineering in Popular Culture

    Type
    Blog Post
    ... it inspired. CAUTION: Spoilers alert! Pre-DNA Era  H.G. Wells, the father of scientific fiction, was... is now famed as the Griffith’s experiment.  DNA Era The period between 1940-1960 was pivotal for genetics...authors during this period.  Genetic Engineering Era: Pre-CRISPR  Having visualized and understood the...preference than the ZFNs.   Genetic Engineering Era: Post CRISPR  CRISPR The strategy of using a protein...molecular genetics was booming, SciFi saw the rise of literary giants such as Isaac Asimov, Arthur C. Clarke,...nucleases (ZFNs) are artificial restriction enzymes generated by fusing a zinc finger DNA-binding domain to ...finger recognizes a nucleotide triplet and when several ZFNs are strung together, it can read many base...

  12. Adenoviral Vector Production and Troubleshooting

    Type
    Blog Post
    ...Potential applications for gene and cell-based therapies in the new era of personalized medicine (2017). ...Potential applications for gene and cell-based therapies in the new era of personalized medicine." Genes & diseases...nucleocapsid. B) Three generations of adenoviral vectors: the first generation vectors are deficient in...in trans. The Gutless AdV have generated high interest for gene therapy due their increased cloning capacity...attractive vectors for research applications and gene therapy: they can be produced at high titers, can accommodate...the Adv genome’s utility in research and its therapeutic potential. Note: constructs discussed are based...based on Adenovirus type 5. Three generations of adenoviral vectors The adenovirus virion is a non-enveloped...

  13. Capturing a Moment with Marc Zimmer’s “The State of Science”

    Type
    Blog Post
    ... trusting science in a “fake news and post-truth era,” the politicization of science (from climate change...intellectual prowess and scientific skills. This is an era of social media and self-promotion, and personality...contributed to the steadily increasing distrust the general public has in science and scientists (as discussed...have addressed CRISPR frequently over the last several years, but lay readers may be less familiar with... interesting case studies, and Zimmer provides several. In Part Three of the book, called simply “Old ...

  14. Designing Your Chalk Talk for the Academic Job Interview

    Type
    Blog Post
    ..., a chalk talk is quite simple. It's you, a dry erase board, some markers (chalk is rare these days), ...of your chalk talk Bring your own fresh set of erasable markers. You’ll have the colors you want and you...in 1-5 years, and contribute to resolving the overarching knowledge gap. Aims should have short titles,.../goal, an approach, anticipated outcomes, and preferably a description of the payoff if the aim is successful...bioengineered scaffolds with patient stem cells to generate compatible functional livers.” Why are you the...getting back to your story. Committee questions can derail your talk. You need to balance respectfully answering... for presenting a science talk Check out our transferable skills guide Read all of our career blog posts...

  15. Open Enzyme Collection

    Type
    Collection
    ... DNA Polymerase I 165533 pOpen-TthpolA Tth DNA polymerase 165534 pOpen-T7gene5 T7 DNA Polymerase (unmodified... Ligases DNA Polymerases Restriction Endonucleases Reverse Transcriptases RNA Polymerases Other Enzymes... of molecular biology, including DNA polymerases, RNA polymerases, DNA ligases, reverse transcriptases... DNA Polymerases Addgene ID Plasmid Gene/insert 165500 pOpen-9N7polA 9 degrees N-7 DNA polymerase 165501.... Heat-Stable DNA Polymerase 165502 pOpen-9N7polA (CT) 9 degrees N-7 DNA Polymerase chain terminating ...pOpen-K12polLF DNA Polymerase I, Large (Klenow) Fragment 165522 pOpen-K12polLF (exo-) DNA Polymerase I, Large (...

  16. Trimmer Lab NeuroMab Collection

    Type
    Collection
    ...channel Mouse Chimera: Mouse/Rat rat IgG2a 222182 RGS14 [N133/21R-rat IgG2a] RGS14 Rat Chimera: Mouse/Rat...receptor Rat Chimera: Mouse/Rat rat IgG2a 222188 GFP [N86/8R-rat IgG2a] GFP Aequorea victoria Chimera: Mouse... to the research community. Protocols for the generation R-mAbs plasmids, production of R-mAbs in transfected...researchers will be able to easily and inexpensively generate R-mAbs by transiently transfecting the plasmids...channel [K14/16R-rat IgG2a] Kv1.2 K+ channel Human Chimera: Mouse/Rat rat IgG2a 222178 CASPR/Neurexin IV [... [K65/35R-rat IgG2a] CASPR/Neurexin IV Rat Chimera: Mouse/Rat rat IgG2a 222179 GluA2/GluR2 glutamate receptor...-rat IgG2a] GluA2/GluR2 glutamate receptor Rat Chimera: Mouse/Rat rat IgG2a 222180 Slo1/BKAlpha maxi-K...

  17. CRISPR Guide

    Type
    Collection
    ...using dual nickases to generate a knockout or gene edit; or using Cas9 to generate large genomic deletions...proteins. Large deletions generated by Cas3 have fewer off-target alterations, such as small mutations ...nucleases (TALENs) required scientists to design and generate a new nuclease pair for every genomic target. ..., and precisely edit DNA and RNA. The ease of generating gRNAs makes CRISPR one of the most scalable genome...and some of the ways the CRISPR components are leveraged to achieve specific goals, such as targeting multiple...depends on which Cas protein you use, and there are several to choose from. Many variations of Cas9 exist, ...will form a ribonucleoprotein complex through interactions between the gRNA scaffold and surface-exposed...

  18. Plasmids for Stem Cell Research

    Type
    Collection
    ...collection of plasmids for the generation and study of stem cells, from iPSC generation to directed differentiation...source for therapeutic use. Since 2006, scientists have developed many approaches to generate iPSCs. The... post Delivery Methods for Generating iPSCs provides an overview of several different reprogramming methods...Non-viral Method to Generate Integration-Free Human iPS Cells from Cord Blood and Peripheral Blood Cells. Stem... Stem Cell Research Plasmids Stem cells are proliferative, unspecialized cells with the capacity to differentiate...pluripotent or multipotent, based on their ability to generate different cell lineages. Pluripotent stem cells... for drug development, disease modeling, and regenerative medicine. iPSCs, much like their ES cell cousins...

  19. Plan Your Experiment

    Type
    Collection
    ...want to: Generate complete and permanent loss of gene expression or function (knockout)? Generate a specific... for different organisms. This page provides a general framework to get you started using CRISPR in your...genetic edit. Figure 1: Flow chart describing the general framework of a CRISPR experiment. Created with ...Manipulation Application Cas Enzyme Additional Considerations Browse Plasmids Knockout Permanently disrupt...verified Cut Nick Homology Directed Repair (HDR) Generate specific genomic edits, like point mutations or...efficiency compared to NHEJ knockout Cut Nick Base Edit Generate specific base pair edits without double-strand...proximal to the PAM sequence Base Edit Prime Edit Generate targeted insertions, deletions, and point mutations...

  20. Bacterial Expression Systems

    Type
    Collection
    ...Protein–Protein Interactions Easy Split Fluorescent Proteins for Studying Protein–Protein Interactions Return ...transcription factors. Addgene Blog Luciferase Technologies Enabled by NanoLuc® Luciferase Fluorescent Biosensors ...purification, protein visualization, study of protein interactions, controlled gene expression, reporter plasmids...Protein Purification Protein Visualization Protein Interactions Controlled Expression Reporter Plasmids Genome...function, structure, and activity of a protein it is generally necessary to purify it. Specific bacterial strains...Fluorescent Protein Timers Return to top Protein Interactions Förster resonance energy transfer (FRET) and...have been widely used to study protein–protein interactions using fluorescence microscopy. FRET relies on...
Showing: 41 - 60 of 1005 results