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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 609 results for: GAN

Showing: 41 - 60 of 609 results

  1. Distribution to Industry

    Type
    Collection
    ...account and affiliate it with your organization. If your organization has not ordered from Addgene in the...fax, or email. Pricing Addgene is a nonprofit organization dedicated to providing the scientific community...

  2. Promega Plasmid Collection

    Type
    Collection
    ...interchangeable ligands. HaloTags form a covalent bond with these synthetic chemical ligands, which have ...and functional studies. You can even customize ligands for your specific purpose. HaloTag technology is...

  3. Genetic Code Expansion

    Type
    Collection
    ...Azf) C. elegans ATG David Tirrell 62599 pKPY197 phenylalanyl-tRNA synthetase (CePheRS) C.elegans p-azido-L-phenylalanine...pairs of synthetase and tRNA that work for one organism may not work for another. The orthogonal synthetase...p-azido-L-phenylalanine (Azf) C. elegans ATG David Tirrell 63177 pMarsL274G methionyl-tRNA synthetase (L274GMmMetRS...

  4. CRISPR Plasmids - Empty gRNA Vectors

    Type
    Collection
    ...Empty sgRNA) 47549 C. elegans yes, cut S. pyogenes Goldstein DR274 42250 C. elegans BsaI none S. pyogenes...Species Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish Xenopus CRISPR Resources CRISPR...Alternatively, gRNA-only plasmids can be used in cells or organisms in which Cas9 has been integrated into the genome...

  5. CRISPR Guide

    Type
    Collection
    ...knock out target genes in various cell types and organisms, but modifications to various Cas enzymes have...be added to detect the presence of infectious organisms (like SARS-CoV-2 ) and genetic mutations. Similar...SpCas9 EQR variant 3' NGAG SpCas9 VQR variant 3' NGAN or NGNG xCas9 3' NG, GAA, or GAT SpCas9-NG 3' NG...Hultquist, J. F., Waters, C. S., McGregor, M. J., Krogan, N. J., & Bondy-Denomy, J. (2017). Inhibition of..., E. P. R., Lin, S., Kiani, S., Guzman, C. D., Wiegand, D. J., Ter-Ovanesyan, D., Braff, J. L., Davidsohn...23907171 Nuñez, J. K., Chen, J., Pommier, G. C., Cogan, J. Z., Replogle, J. M., Adriaens, C., Ramadoss,...

  6. Neurodegeneration Research Collection

    Type
    Collection
    ... for whole-brain neuronal identification in C. elegans. Yemini et al. Cell. 2021 Jan 7. Use a FRET-based...monitor structural transitions in protein self-organization. Wan et al. Cell Rep Methods. 2022 Mar 28. Follow...CHDI Foundation (Link opens in a new window) An organization with a mission to rapidly develop therapeutics...

  7. Cre-Lox and Other Site-Specific Recombinases

    Type
    Collection
    ... the expression of specific genes in cells or organisms. Each recombinase enzyme recognizes and binds ...interest. Inducible: The recombinase may be fused to a ligand-binding domain such as the human estrogen receptor...sequence of the recombinase to align with your organism's preferred codon usage will improve expression...

  8. CRISPR Pooled gRNA Libraries

    Type
    Collection
    ...particular organism, and the library is only compatible with cells derived from that organism. All of our...Species Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish Xenopus CRISPR Resources CRISPR...

  9. Synthetic Biology - Worm

    Type
    Collection
    ...collection of synthetic biology plasmids for use in C. elegans....collection of synthetic biology plasmids for use in C. elegans. Worm Plasmids Search the table by keyword or sort...

  10. Cancer Research Plasmids and Resources

    Type
    Collection
    ...curated collection of plasmids for cancer research, organized by signaling pathway. ...constructs. Each collection page listed below organizes the plasmids and resources by signaling pathway...

  11. Chemogenetics Plasmids

    Type
    Collection
    ...PSAMs are coupled to an ion pore domain to create a ligand-gated ion channel. Whether a PSAM is activating...detailed descriptions of chemogenetic receptors, ligands, and how to use these tools in your experiments...

  12. Resolute Plasmid Collection

    Type
    Collection
    ...and changes of voltage all contribute to assess ligand action/engagement. Protein production is used for...assays and to generate antibody/ high-affinity ligands. Data is embedded in an integrated knowledgebase...

  14. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ...loci can be easily expressed in the same cell or organism. The ability to precisely target CRISPR to a given...others) Drosophila Plants (monocots and dicots) C. elegans Yeast ( S. cerevisiae and S. pombe ) Zebrafish ...

  15. Plan Your Experiment

    Type
    Collection
    ...researchers can adapt these experiments for different organisms. This page provides a general framework to get...principles also apply to using CRISPR in other organisms. As with most experiments, you first need to have...

  16. Antibody Guide

    Type
    Collection
    ...myeloma cells (immortalized B cells). The genetic reorganization that creates variety in the antibody allowing...the variable region goes through a randomized reorganization process, creating a unique binding site. This...

  17. Live and Let Dye: Self-Labeling Protein Tags

    Type
    Blog Post
    ...systems Tag Size (amino acids) Ligand Commercial ligands available Ease of use Tetracysteine...though. Purchasing lots and lots of organic dyes or specialized ligands is more expensive than letting the... synthesizing your own ligands brings up bad memories of undergraduate organic chemistry lab, don’t worry...linked to, say, a chemical dye or other functional ligand, your protein of interest will end up linked to...Just express the fusion protein and add a specific ligand! A self-labeling protein gives you the flexibility...flexibility to choose different colors or ligands for different experiments without having to clone a whole ...structure represents a fluorophore or other useful ligand, gray structure is the protein of interest, and...

  18. The CRISPR Software Matchmaker: A New Tool for Choosing the Best CRISPR Software for Your Needs

    Type
    Blog Post
    ...to one or a subset of organisms. Examples: “organism agnostic”, “model organisms”, “small genome”, “large...require the organism to be specified and are truly organism agnostic. Other tools require an organism to be ...of 2014, tools began moving away from designing exclusively for NGG PAM targets and began allowing for ...only one, or a subset of organisms. In only a few cases the software is organism agnostic. Most tools are...tools will be marked as organism agnostic and have currently available organisms listed in this section...some kind of pre-analysis. At the end of 2014, I began developing software to make sgRNA design accessible...input types can be readily converted.  Examples: “organism”, “sequence”, “identifier”, and “load”. Trends...

  19. Transferable Skills Guide: External Collaborations

    Type
    Blog Post
    ...to mid-sized companies and organizations. Addgene as a nonprofit organization is very considerate and thoughtful... outside an organization The next step is to expand your efforts beyond your organization and/or scientific...people from various organizations in science and beyond. For example, I help organize scientific workshops...guide: Collaborating with others outside your organization also known as external collaborations. Interestingly...the ability to work with others outside one’s organization was found in the aforementioned survey as one... partnerships where we can leverage another organization’s expertise to our mutual benefit.  Our partnership...small: Collaboration opportunities within an organization but outside your comfort zone As part of my ...

  20. An Integrin Antibody Toolkit from IPI

    Type
    Blog Post
    ...with low affinity for ligands. When integrins interact with extracellular ligands, their ectodomains can... binding ligands with high affinity. This extended-open state is key to strengthening ligand binding, ...almost all bind outside the ligand-binding pocket, their sheer size impeding ligand attachment. The five other...often express multiple integrins that bind the same ligand, making it difficult to dissect the function of... antibodies in the collection are able to block ligand binding — and therefore interrupt integrin function...ascertain which integrins are responsible for specific ligand-binding functions, all key to basic biomedical ...intracellular ends and large ectodomains containing ligand-binding heads and long legs that protrude from ...
Showing: 41 - 60 of 609 results