...Now, scientists are beginning to see Addgene as a large data set. Addgene has over 65,000 plasmids in the...protein, their gene sequence may have slight or large variations depending on how closely related these...the only time researchers have used Addgene as a large data set. Alec A. K. Nielsen & Christopher A. Voigt...within the Addgene repository is mammalian, but the largest source of unique gene sequences is Proteobacteria...

... is observed. Today Southern blotting has been largely replaced by real-time PCR to answer the same experimental...Southern blotting is labor intensive and requires a large amount of high-quality DNA, real-time PCR has several...discoveries (5, 8). While newer techniques have largely replaced these, these techniques still have useful...bring efficient, low cost methods for sequencing large amounts of DNA quickly and accurately. We're sure...PMCID: PMC4727787. 9. Hunkapiller, Tom, et al. "Large-scale and automated DNA sequence determination."...

...insert your gene of interest into a vector with a large tag at the N-terminus, but you don’t have the tag...fairly high, so there is usually no need to screen a large amount of colonies. Excluding the time it takes ...those where the vector that you want to use is very large, has a high GC content, contains a lot of repeats...described recently (Jiang et al. 2015), in which very large fragments of the bacterial chromosome (up to 100...segments CATCH enables one-step targeted cloning of large gene clusters. Nat Commun. 2015; Sep 1;6:8101. PubMed...

..., SeeDB2 is not powerful enough for large brain samples. For large brain samples, we recommend pretreatment... to achieve highest expression levels. Clearing large tissues - Fluorescent proteins are very stable in...

... Numbers in the large colored circles are rough approximations of the total number of CRISPR plasmids...of insert) and the reduced titer associated with large inserts can make lentivirus production frustrating... titer of her prep makes it unuseable. With the large size of the SpCas9 nuclease (~4.2 kb) this is a ...

...are available for making small point mutations, large deletions/insertions require complicated, often ...possible to use the same technique just using a large primer known as a megaprimer. Piecing together multiple...L-arabinose. These cells can be made and frozen in large batches suitable for many experiments. When REPLACR-mutagenesis...

...nearly 100% in archaea, though Class 1 remains largely underutilized compared to Class 2. Class 1 Despite... type of CRISPR overall. Type I systems degrade large sections of DNA thanks to the dual action power ...As you might imagine, this is handy for creating large genomic deletions. Type I systems are also employed...technically target both RNA and DNA, though it is largely believed that the cleavage of DNA is the primary...CRISPR-associated transposase (CAST) that can insert large fragments of DNA. The V-F variants, which are among...will no doubt keep increasing, as efforts using large-scale clustering and discovery algorithms find new...

...can sometimes go unnoticed at large organizations. Another downside to large commercial companies which ...samples in the lab, but I really struggled with the large scale data analysis, as this is increasingly reliant...There were, however, some downsides to working in a large commercial company. In my particular situation, ... seemed to create more bureaucracy. As with any large company (both for-profit and non-profit) you can... companies. It was really exciting to work in a larger commercial company; the pay was good and there ... changes were welcomed, which is a benefit over larger companies which have more bureaucracy and cannot...

...James from Binning Singletons. The sheer scale of a large conference can be intimidating. And it can be exacerbated...structure and support network for people attending large meetings. A key was to provide each bin with a mentor... the participants to feel more comfortable at a large meeting. They had the confidence to meet someone...-meeting survey indicated that the program was largely a success, but also helped us find areas for improvement...

...currently known to cause any diseases. Recombinant AAV largely does not integrate, and the addition of a helper... that of lenti and gamma-retro viruses. This is large enough for many applications, but in some cases ...transient expression of a genetic cargo or have a very large cargo. Fast fact: All viruses discussed in this...have been derived from. Lentiviruses have slightly larger packaging capacities than gamma-retrovirus, coming...

...in the host. Adenoviruses can deliver relatively large cargo inserts (>8 kb). Adeno-associated viruses...subject delivery, as immunogenicity was previously a large barrier for gene therapy. In fact, AAV gene therapies...High Gene Expression Infects Non-Dividing Cells Large Packaging Capacity (>8kb) High Transduction Efficiency...

... the backbone into the insert. If the insert is large, we may only check the 5’ and 3’ ends to verify ...which is critical for the efficient handling of large numbers of samples. When we grow overnight cultures...submission instructions. As for the analysis of a large number of sequencing reactions, you can make your...

...entire axial volume of a sample. Therefore, when a large number of optical slices are acquired all fluorophores...they require both optical sectioning, imaging over large distances and high temporal resolution. Traditionally...photobleaching when acquiring multiple images over a large axial range. 2-photon imaging, which restricts excitation....0NA objective with proper sampling. Therefore, large, cleared tissues need to be imaged via Lightsheet...this size, those that can will completely image large tissues in a few hours. Although the modern explosion...

...vertical.  Large central box. Another commonly used and effective style is one employing a large central ...is written across the center of the poster in a large font. Hence, spectators can read the text from many...written and graphical information are inserted into larger circles or ovals, that are arranged in some logical...

...short life cycle of 8 to 14 days, and produce a large number of offspring that are laid externally. Fruit... small, easily maintained as they are housed in large groups, easily bred, and produce 50-300 eggs at ...transparent worms that have a rapid life cycle and large brood sizes. The majority of C. elegans are also...self-fertile hermaphrodites making it easy to conduct large experiments over multiple generations. Similarly...

... scattered by that cell at a low angle (FSC) or large angle (SSC) (Figure 1). FSC values depend on the...forward at a low angle (FSC) or to the side at a large angle (SSC). FSC and SSC depend on the cell’s size...cell population will show up on an FSC/SSC plot, largely depends on cell type and laser voltage. The voltage...populations displayed. As the separation pattern largely stays the same and does not impact my ability to...population distinct from cellular debris and other larger cells. Note, we don’t mind the warning message ...

..., the Rat Genome Sequencing Project conducted a large scale sequencing and concluded that nearly all disease-linked...relatively simple to maintain and breed and they have large, easily modifiable embryos, which is ideal for observing...attractive features are their eggs. Frog eggs are large, opaque, and produced in abundance, making them ...make an ideal model organism: quick development, a large number of offspring (produces many seeds), self ...disease mode (Iannaccone et al., 2009). Rats are larger than mice making them more useful for certain studies...

.... Plasmids In addition to EYFP and ChR2-EYFP, a large number of additional, validated molecular payloads...

...is often the case with large genes. This is due to inefficient packaging of large inserts; a study by Kumar...start. If you don’t anticipate that you will need large volumes of lentivirus routinely, you may opt for... guides) while a single 72h harvest is best for larger genes such as Cas9. See figure 1 for an example...dishes or increasing the vessel size. Opting for a larger vessel, such as a 3 or 5-layer flask will require...calculating the ratio of the surface area of the larger vessel to the 10 cm2 dish and adjusting the cell...

...targeting constructs. In the example shown in Figure 1, large sequences corresponding to exons 1 and 3 of the ... the cells that have randomly integrated all or large portions of the plasmid. Deliver your construct ...CRISPR is very good at making knockouts, knocking in large sections of DNA can be more difficult. Addgene depositors...