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We narrowed to 492 results for: LARGE

Showing: 41 - 60 of 492 results

  1. The Stingy Scientist: How the Baby Gel Box Was Born

    Type
    Blog Post
    ...powder pruchased from a pottery supply house.  These large, cheap aliquots could be stored indefinitely at ...science project started when I realized I was pouring large agarose gels for DNA visualization or isolation,...amount of agarose, I could pour a very thin gel on a large glass slide using only surface tension.  It's a ...

  4. Plasmids 101: Protein Expression

    Type
    Blog Post
    ...expression systems can be scaled up for production of large amounts of protein. Some drawbacks of insect cell...popular DNA vectors that can be used to produce large amounts of protein in bacterial cells: the pET, ...Yeast are a great expression system to generate large quantities of recombinant eukaryotic proteins. Although... be easily adapted to fermentation allowing for large-scale, stable production of proteins. In general...

  5. What the HEK?

    Type
    Blog Post
    ...293, the most commonly used line expresses SV40 large T antigen and is thus dubbed HEK-293T. Given that... viral production because they express the SV40 large T-antigen. This allows them to replicate viral plasmids...HEKs can also grow in suspension, which allows for large scalability, and can also be adapted for serum-free...

  6. CUT&RUN: An Improved Method for Studying Protein-DNA Interactions

    Type
    Blog Post
    ...Problems with Chip-seq ChIP-seq typically requires a large amount of input material, cells or tissue, to produce...with cell culture, because it is easy to harvest large quantities of cells. However, when working on specific...situations when it is technically challenging to collect large numbers of cells or when a high degree of signal...

  7. Natural or synthetic? How Addgene’s dataset reveals trends in biological innovation

    Type
    Blog Post
    ...Now, scientists are beginning to see Addgene as a large data set. Addgene has over 65,000 plasmids in the...protein, their gene sequence may have slight or large variations depending on how closely related these...the only time researchers have used Addgene as a large data set. Alec A. K. Nielsen & Christopher A. Voigt...within the Addgene repository is mammalian, but the largest source of unique gene sequences is Proteobacteria...

  8. Evolution of Lab Techniques

    Type
    Blog Post
    ... is observed. Today Southern blotting has been largely replaced by real-time PCR to answer the same experimental...Southern blotting is labor intensive and requires a large amount of high-quality DNA, real-time PCR has several...discoveries (5, 8). While newer techniques have largely replaced these, these techniques still have useful...bring efficient, low cost methods for sequencing large amounts of DNA quickly and accurately. We're sure...PMCID: PMC4727787. 9. Hunkapiller, Tom, et al. "Large-scale and automated DNA sequence determination."...

  9. Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods

    Type
    Blog Post
    ...insert your gene of interest into a vector with a large tag at the N-terminus, but you don’t have the tag...fairly high, so there is usually no need to screen a large amount of colonies. Excluding the time it takes ...those where the vector that you want to use is very large, has a high GC content, contains a lot of repeats...described recently (Jiang et al. 2015), in which very large fragments of the bacterial chromosome (up to 100...segments CATCH enables one-step targeted cloning of large gene clusters. Nat Commun. 2015; Sep 1;6:8101. PubMed...

  10. Tetbow: Bright Multicolor Labeling for Neuronal Tracing

    Type
    Blog Post
    ..., SeeDB2 is not powerful enough for large brain samples. For large brain samples, we recommend pretreatment... to achieve highest expression levels. Clearing large tissues - Fluorescent proteins are very stable in...

  11. With Our New Viral Service, We're Taking CRISPR Further!

    Type
    Blog Post
    ... Numbers in the large colored circles are rough approximations of the total number of CRISPR plasmids...of insert) and the reduced titer associated with large inserts can make lentivirus production frustrating... titer of her prep makes it unuseable. With the large size of the SpCas9 nuclease (~4.2 kb) this is a ...

  12. REPLACR Mutagenesis: Replacing In Vitro Recombination Methods

    Type
    Blog Post
    ...are available for making small point mutations, large deletions/insertions require complicated, often ...possible to use the same technique just using a large primer known as a megaprimer. Piecing together multiple...L-arabinose. These cells can be made and frozen in large batches suitable for many experiments. When REPLACR-mutagenesis...

  13. CRISPR Plasmids - Base Edit

    Type
    Collection
    ...Publication Return to top CRISPR Resources Addgene has a large selection of CRISPR plasmids and resources. Find...

  14. Typing CRISPR Systems

    Type
    Blog Post
    ...nearly 100% in archaea, though Class 1 remains largely underutilized compared to Class 2. Class 1 Despite... type of CRISPR overall. Type I systems degrade large sections of DNA thanks to the dual action power ...As you might imagine, this is handy for creating large genomic deletions. Type I systems are also employed...technically target both RNA and DNA, though it is largely believed that the cleavage of DNA is the primary...CRISPR-associated transposase (CAST) that can insert large fragments of DNA. The V-F variants, which are among...will no doubt keep increasing, as efforts using large-scale clustering and discovery algorithms find new...

  15. Finding Your Perfect Job After University

    Type
    Blog Post
    ...can sometimes go unnoticed at large organizations. Another downside to large commercial companies which ...samples in the lab, but I really struggled with the large scale data analysis, as this is increasingly reliant...There were, however, some downsides to working in a large commercial company. In my particular situation, ... seemed to create more bureaucracy. As with any large company (both for-profit and non-profit) you can... companies. It was really exciting to work in a larger commercial company; the pay was good and there ... changes were welcomed, which is a benefit over larger companies which have more bureaucracy and cannot...

  16. Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone

    Type
    Blog Post
    ...James from Binning Singletons. The sheer scale of a large conference can be intimidating. And it can be exacerbated...structure and support network for people attending large meetings. A key was to provide each bin with a mentor... the participants to feel more comfortable at a large meeting. They had the confidence to meet someone...-meeting survey indicated that the program was largely a success, but also helped us find areas for improvement...

  17. Viral Vectors 101: Types of viruses

    Type
    Blog Post
    ...currently known to cause any diseases. Recombinant AAV largely does not integrate, and the addition of a helper... that of lenti and gamma-retro viruses. This is large enough for many applications, but in some cases ...transient expression of a genetic cargo or have a very large cargo. Fast fact: All viruses discussed in this...have been derived from. Lentiviruses have slightly larger packaging capacities than gamma-retrovirus, coming...

  18. Viral Vectors 101: Viruses as Biological Tools

    Type
    Blog Post
    ...in the host. Adenoviruses can deliver relatively large cargo inserts (>8 kb). Adeno-associated viruses...subject delivery, as immunogenicity was previously a large barrier for gene therapy. In fact, AAV gene therapies...High Gene Expression Infects Non-Dividing Cells Large Packaging Capacity (>8kb) High Transduction Efficiency...

  19. Addgene's Tips for Plasmid Quality Control

    Type
    Blog Post
    ... the backbone into the insert. If the insert is large, we may only check the 5’ and 3’ ends to verify ...which is critical for the efficient handling of large numbers of samples. When we grow overnight cultures...submission instructions. As for the analysis of a large number of sequencing reactions, you can make your...

  20. Which Fluorescence Microscopy Technique is Best for Me?

    Type
    Blog Post
    ...entire axial volume of a sample. Therefore, when a large number of optical slices are acquired all fluorophores...they require both optical sectioning, imaging over large distances and high temporal resolution. Traditionally...photobleaching when acquiring multiple images over a large axial range. 2-photon imaging, which restricts excitation....0NA objective with proper sampling. Therefore, large, cleared tissues need to be imaged via Lightsheet...this size, those that can will completely image large tissues in a few hours. Although the modern explosion...
Showing: 41 - 60 of 492 results