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Showing: 41 - 60 of 857 results

  1. Plasmid Cloning by PCR (with Protocols)

    Type
    Protocol
    ...amplify and design primers that will bind to and replicate it. The following image shows the ends of the ...mutation than restriction enzyme based cloning. DNA replication by PCR has error rates that range from roughly...

  2. Western Blot

    Type
    Protocol
    ...BSA standard that range from 0–2000 µg/mL . In duplicate, dilute 10 µL of standard, blank, and lysate samples... nm . Calculate the average absorbance of the duplicate samples on the plate. Subtract the average absorbance...

  3. DNA Quantification

    Type
    Protocol
    ...mini-prepped DNA onto the pedestal. Close the lid and click measure, be sure to record the concentration and...

  6. Centrifugation

    Type
    Protocol
    ...according to speed and time (and temperature, if applicable) listed in your protocol. Pro-Tip Spin speed ...

  7. General Transfection

    Type
    Protocol
    ...DNA. Add the diluted PEI dropwise while gently flicking the diluted DNA tube. Incubate the mixture 15–...

  9. Gibson Assembly Protocol

    Type
    Protocol
    ...Link opens in a new window) . Gibson Assembly® is licensed to New England Biolabs by TelesisBio. Gibson DG...

  10. Immunocytochemistry

    Type
    Protocol
    ...as methanol or acetone may be better for some applications. Remove the paraformaldehyde and follow your...

  11. Colony Formation Titering Assay

    Type
    Protocol
    ...lentiviral preparation allows you to control the multiplicity of infection (MOI) in downstream studies. This...

  15. AAV ddPCR Titration

    Type
    Protocol
    ...quantification. This protocol was modified from the publication Lock et al. (2014) (Link opens in a new window...

  16. AAV Production in HEK293 Cells

    Type
    Protocol
    ... layers are covered with media. 293T cells are delicate and detach very easily - media should always be...

  17. Lentivirus ddPCR Titration

    Type
    Protocol
    ... sample. This protocol was modified from the publication Wang et al. (2018) . Before Starting Thaw the...

  18. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...sensor, cytosolic or ER-targeted eZinCh-2: A Versatile, Genetically Encoded FRET Sensor for Cytosolic and ...and small molecule indicator: implications for quantification of cytosolic Zn(2+). ACS Chem Biol. 2013 ...:7351. Tetsuya Kitaguchi cAMP (cyclic AMP) Fluorescent sensor of cyclic AMP (cAMPr) for mammalian or insect...sensor for cyclic AMP. Sci Signal. 2018 Mar 6;11(520). pii: 11/520/eaah3738. Justin Blau cAMP (cyclic AMP) ...:4003-4012.e6. Xavier Nicol cyclic di-GMP FRET-based biosensor for cyclic-di-GMP measurement Asymmetrical...Fluorescent biosensor of the cytosolic NADH/NAD+ redox state Imaging Cytosolic NADH-NAD(+) Redox State with...protein for NADH/NAD+ ratio Cytosolic NADH-NAD Redox Visualized in Brain Slices by Two-Photon Fluorescence...

  19. Antibody Guide

    Type
    Collection
    ...single assay. Overview of Antibody Applications Antibody-based applications can be generally classed into ...these applications, visit the Antibody section of the Addgene Protocols page. Antibody Applications - Quantification...production and storage techniques, and explain common applications. Science... Handling Visualization Signal Amplification Application Overview Quantification Methods Capture Methods...of other antibodies. This has useful research applications, and is covered in the following Isotypes section...signaling molecules and are often used in clinical applications. Diabodies - Diabodies contain two Fab fragments... molecules that can be distinguished in your application. The indirect detection method uses an unconjugated...

  20. CRISPR Plasmids - Mammalian Expression

    Type
    Collection
    ...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Browse CRISPR Plasmids By Function Genome Editing...RNA Targeting RNA Targeting RNA Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Nick CRISPR/Cas nickase mutants introduce gRNA-targeted...Plasmid Gene/Insert Promoter Selectable Marker Publication Prime Edit Cas9 H840A nickase fused to a reverse...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Activate Catalytically dead dCas9 fused to a ...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Interfere Catalytically dead dCas9, or dCas9 ...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Epigenetics To make targeted epigenetic modifications...
Showing: 41 - 60 of 857 results