...neuronal manipulation. Figures, tables, data, or images on best practices (e.g., dose response data, list...

...somatic cells, which makes them attractive for imaging and fluorescence microscopy experiments. The C....

...dCas9 that is fused to 2x NLS and EGFP for CRISPR imaging 51024 pSLQ1651-sgTelomere(F+E) Lentiviral vector...

...Link opens in a new window) , including: data and images from the hiPS cell lines gRNA sequences used in...

...December 2011 Newsletter Overview of TALEN Technology Imagine being able to manipulate a specific region of DNA...

...Enzyme collection contains enzyme genes for fixing damaged and degraded DNA prior to amplification, enzymes...

...application TMN200 (200 mM sodium chloride, 1 mM magnesium chloride, 20 mM Tris at pH 8.0) Suitable for in...

...corresponding catalog item (see maps section for images). Figure 2: AAV Pro cells were transduced with ...

...improved activation kinetics and localization for imaging synaptic transmission. Aggarwal et al. Nat Methods...

...SARS-CoV-2 is primed by the serine protease TMPRSS2. Image credit: Maya Kostman for the Innovative Genomics...

... information, prenatal LMD microarray data, ISH image data, and anatomic reference atlases of prenatal...

...gene regulatory element; NHP, non-human primate. Image courtesy of the Gradinaru Lab and the Caltech CLOVER...

...!), photoactivatable tags for super resolution imaging, epitope tags like His for easy western blotting...

...brightness, assessed using ssRNAseq or high-resolution imaging. For more details, visit the Allen Institute's ...

...none Flp dependent 8 Deisseroth 158757 AAV-CAG-SomaGCaMP6f2 CAG soma-GCaMP6f2 none Constitutive 8, 9 Boyden...

...non-dividing cells for a period of years without damaging the host cell. In contrast, viral particles produced...

... at Addgene. Sherf, et al. 1994. Promega Notes Mag. Giannakis, et al. BioTechniques. 2003. PubMed ...Nano-lanterns for multicolor luminescence imaging  Fluorescent imaging techniques have become indispensable...):198-200. SunTag system for single molecule imaging and inducible gene expression Brilliant technologies...have adopted and employed this concept in both imaging and inducible gene expression studies, which are...advanced technology in the fields of single molecule imaging and inducible gene expression by creating the SunTag...system, cleverly termed TRICK, for Translation RNA Imaging by Coat protein Knock-off, is made up of three ...developed a CRISPR-based multicolor tool enabling the imaging of multiple endogenous genomic loci simultaneously...

...x-ray, or a biologist imaging the smallest structures of life; the key to a great image is contrast. The human...Epi-fluorescence microscopes. (See left image above for an example image). Point Scanning Confocal Microscopy...the speed at which a confocal image can be acquired. Assembling an image pixel by pixel is slow; therefore... challenging samples to image as they require both optical sectioning, imaging over large distances and...can push imaging depths closer to 1 mm. Traditionally, 2-photon has been a very slow imaging technique...Lightsheet designs that allow for multi-view imaging can image thick scattering samples in their entirety...photobleaching when acquiring multiple images over a large axial range. 2-photon imaging, which restricts excitation...

...open the offending red-green image, go to Image>Color>Replace Red with Magenta. Or you can use Color>Split...wash again, then image. But here’s where the “sequential” part comes in. After you image the first target....1 (magenta), anti-AnkyrinG (green), and Hoechst nuclear stain (cyan). Panels C1-C3 show magnified detail...ve captured your images, various software programs will allow you to align the images of each labeled ...maybe you just want to make some really beautiful images? Multiplex IF may be just what you need! How does...of primary and secondary antibody labeling and imaging, repeating for each target. Alternatively, you ...Sequential Multiplex IF, where one target is labeled and imaged at a time, inactivating the fluorescent secondary...

...called Magnetic Isolation and Concentration (MagIC)-cryo-EM that combines enrichment and imaging of proteins...proteins captured on magnetic beads (Arimura et al., 2024). In MagIC-cryo-EM, target proteins (expressed...plasmids for MagIC-Cryo-EM here!   Arimura, Y., Konishi, H.A., Funabiki, H. (2024) MagIC-Cryo-EM: Structural...articles. Here's what you'll find in this post: MagIC-Cryo-EM for enrichment and structure determination...Recombinant antibodies for marking astrocytes MagIC-Cryo-EM for sample enrichment and structure determination...proof-of-concept study) are captured on streptavidin-coated magnetic beads through an assembly of biotinylated SpyTag-SpyCatcher...and minimizes sample loss while also supporting imaging, making it easy to identify dozens or hundreds ...