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We narrowed to 113 results for: PEP

Showing: 41 - 60 of 113 results
  1. Antibodies 101: Fab Fragments

    Type
    Blog Post
    ...IgG antibody (Figure 1): it is composed of four peptide chains (two identical heavy chains and two identical...recombinantly and engineered to be joined by a linker peptide, resulting in a product called a single-chain Fragment...
  2. Plasmids 101: Protein Expression

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    Blog Post
    ... can then be translated at the ribosome into polypeptide chains that make up the primary structure of ...recombinant proteins with a naturally secreted plant peptide allows for easier access and purification of a ...
  3. Better Dyeing Through Chemistry & Small Molecule Fluorophores

    Type
    Blog Post
    ...short genetically encoded tetracysteine (Cys4) peptide tag could be used to label proteins in cells (Figure...covalent attachment of a fluorophore ligand to a peptide tag (Figure 1c). Click chemistry (e.g., transcyclooctene–tetrazine...
  4. Neuronal labeling with Spaghetti Monster

    Type
    Blog Post
    ...neuronal circuits. Epitope tags are short antigenic peptide sequences, attached to a protein of interest (POI...simian virus 5-derived epitope (V5), the synthetic peptide FLAG, the synthetic streptavidin-binding strep-...
  5. Hot Biosensors 2022: Year-End Roundup

    Type
    Blog Post
    ...Addgene’s catalog is a new class of sensors for neuropeptides and neurotransmitters. Motivated to develop ...GPCRs recognizing various neurotransmitters, neuropeptides and other ligands - almost A to Z! - from ATP...
  6. Antibodies 101: Single Chain Fragment Variables (scFvs)

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    Blog Post
    ...recombinant antibody. They are ~25 kDa single polypeptides that contain the variable light chain (VL) and... two chains are connected by a flexible linker peptide that is usually 15-20 amino acids long and made...
  7. Using Phosphoserine to Study Protein Phosphorylation

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    Blog Post
    ...extension and translation of the pSer-incorporated polypeptide. These three components were shown to effectively...proportional to a single phosphate moiety on the peptide of interest.  After confirming pSer incorporation...
  8. Viral Vectors 101: AAV Serotypes and Tissue Tropism

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    Blog Post
    ...Laminin receptor (LR)3 AAV receptor (AAVR)2 Carboxypeptidase D (CPD), also called AAVR25   AAV9 Terminal...receptor (LR)3 Putative integrin3 AAV11 Carboxypeptidase D (CPD), also called AAVR25   1Wu, Miller...
  9. Rinehart Lab Phosphoprotein Reagents

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    Collection
    ... incorporates phosphoserine into the growing polypeptide chain to generate the new phosphorylated protein...serine phosphorylation ( Rinehart Human Serine Phosphopeptide Library ). The phosphorylation sites (“phosphosites...purifying the phosphosite library as GST-fusion peptides (“Mode #1” expression). The Mode #1 phosphosite...experiments in need of reference spectra or phosphopeptide standards for quantitative assessments. The...detection of phosphorylation-dependent protein-peptide interactions (“Mode #2” expression, Barber et al...pSerOTS-C1* (V70) (DOCX, 551 KB) Human Serine Phosphopeptide Library described in: Barber, K. W., Muir, ...Rinehart, J. (2018). Encoding human serine phosphopeptides in bacteria for proteome-wide identification...
  10. p53 Pathway

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    Collection
    ...apoptosis-related cysteine peptidase CASP8 Caspase 8, apoptosis-related cysteine peptidase CASP9 Caspase 9, apoptosis-related...Symbol Name 14-3-3-σ Stratifin Apaf-1 Apoptotic peptidase activating factor 1 ATM ATM serine/threonine kinase...apoptosis-related cysteine peptidase Cyclin B CCNB1 CCNB2 CCNB3 Cyclin B1, 2, or 3 Cyclin D CCND1 CCND2... PAG608 Zinc finger, matrin-type 3 PAI Serpin peptidase inhibitor, clade E (nexin, plasminogen activator...
  11. Fluorescent Protein Guide: Biosensors

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    Collection
    ... pii: 56469. Edwin Chapman Neuropeptides GRAB biosensors for neuropeptides VIP, NPY, CCK, NTS, UCN, SST... orexin neuropeptides A genetically encoded sensor for in vivo imaging of orexin neuropeptides. Nat Methods...based on computationally redesigned calmodulin-peptide pairs. Chem Biol. 2006 May;13(5):521-30. Amy Palmer...of FRCaMP (Fungus based Red CalModulin/M13-like Peptide) A sensitive soma-localized red fluorescent calcium... selective and sensitive genetically encoded neuropeptide sensors. Science. 2023 Nov 17;382(6672):eabq8173...exploiting a temperature-responsive elastin-like polypeptide. Sci Rep. 2021 Aug 13;11(1):16519. Takeharu Nagai...
  12. Trimmer Lab NeuroMab Collection

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    Collection
    ...IgG2a 188173 Anti-NPY2R/Neuropeptide Y receptor type 2 [L119/134R] NPY2R/Neuropeptide Y receptor type 2 Human...Parvalbumin Rat Mouse IgG2b 206639 Anti-Neuropeptide Y [L115/13R-2b] Neuropeptide Y Human Mouse IgG2b 206640 Anti-VGAT... 211594 Anti-NPY2R/Neuropeptide Y receptor type 2 [L119/106R-2b] NPY2R/Neuropeptide Y receptor type 2 ...Rat Mouse 190505 NPY/Neuropeptide Y scFv [L115/13] L115/13 scFv 2t NPY/Neuropeptide Y Human Mouse 190506...mouse)/R. norvegicus (rat) rat IgG2a 227066 NPY/Neuropeptide Y (Homo sapiens) recombinant mouse monoclonal...)/R. norvegicus (rat) rat IgG2a 227067 NPY2R/Neuropeptide Y receptor type 2 (Homo sapiens) recombinant...
  13. Bacterial Expression Systems

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    Collection
    ... cleavage sites. Other protein tags and signal peptides are used to enhance protein solubility and to ...Commonly used tags, cleavage sites, and signal peptides include: Epitope tags: 6xHis, Flag, Strep II, ...protease, factor Xa, enterokinase, thrombin Signal peptides for periplasmic localization: PelB, MalE, OmpA...Methylobacterium extorquens Christopher Marx 122635 pPEPZ-Plac Plac Lactose/IPTG Streptococcus pneumoniae ...
  14. Ras Pathway

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    Collection
    ...2 like 2 NFKB1 Nuclear factor of kappa light polypeptide gene enhancer in B-cells 1 PAK PAK1 PAK2 PAK3...catalytic and regulatory subunits - Class I PIN1 Peptidylprolyl cis/trans isomerase, NIMA-interacting 1 PLXNB1...RB1 Retinoblastoma 1 RCE1 Ras converting CAAX endopeptidase 1 RHEB Ras homolog enriched in brain RHO RHOA...
  15. 27 Hot Plasmids from 2016

    Type
    Blog Post
    .... Mark Howarth’s lab recently engineered a new peptide/protein pair, SnoopTag/SnoopCatcher, that can be...link desired proteins together via a spontaneous isopeptide bond. When used in combination with their previously...
  16. Antibodies 101: Affinity Tags

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    Blog Post
    ...This is where affinity tags come in – they are peptides which do not naturally exist in most expression...
  17. Another Pathway into Cells: iTOP

    Type
    Blog Post
    ...transduction strategy is to fuse a cell penetrating peptide (CPP) to a protein of interest thereby giving it...
Showing: 41 - 60 of 113 results