...Addgene’s catalog is a new class of sensors for neuropeptides and neurotransmitters. Motivated to develop ...GPCRs recognizing various neurotransmitters, neuropeptides and other ligands - almost A to Z! - from ATP...

...recombinant antibody. They are ~25 kDa single polypeptides that contain the variable light chain (VL) and... two chains are connected by a flexible linker peptide that is usually 15-20 amino acids long and made...

...extension and translation of the pSer-incorporated polypeptide. These three components were shown to effectively...proportional to a single phosphate moiety on the peptide of interest.  After confirming pSer incorporation...

...Millipore Sigma 12072 Antipain, Millipore Sigma 10791 Leupeptin, Millipore Sigma L2884 Aprotinin, Millipore Sigma...sterilize 1000X protease inhibitor cocktail 25 mg leupeptin 50 mg antipain 250 mg benzamidine 25 mL of 2 mg...

.... Mark Howarth’s lab recently engineered a new peptide/protein pair, SnoopTag/SnoopCatcher, that can be...link desired proteins together via a spontaneous isopeptide bond. When used in combination with their previously...

...like high resolution Ras sensors and crosslinked peptides proposed as a scaffold for a new generation of...

...CCN3, IGFBP9 NPFF neuropeptide FF-amide peptide precursor FMRFAL NPPA natriuretic peptide precursor A ANF...natriuretic peptide precursor B BNP NPPC natriuretic peptide precursor C CNP NPR1 natriuretic peptide receptor...class I polypeptide-related sequence A FLJ60820, MGC111087, PERB11.1 MICB MHC class I polypeptide-related...catalytic, delta polypeptide p110D PIK3CG phosphoinositide-3-kinase, catalytic, gamma polypeptide PI3CG, PI3K..., FGFB, HBGF-2 FPR1 formyl peptide receptor 1 FMLP, FPR FPR2 formyl peptide receptor 2 ALXR, FMLP-R-II...gonadotropin, beta polypeptide CGB3, hCGB CGB1 chorionic gonadotropin, beta polypeptide 1 - CGB2 chorionic... gonadotropin, beta polypeptide 2 - CGB5 chorionic gonadotropin, beta polypeptide 5 HCG, MGC119822 CGB7...

...This is where affinity tags come in – they are peptides which do not naturally exist in most expression...

...type tropism. Introducing changes and/or short peptides sequences into these variable loops is one method...

...transduction strategy is to fuse a cell penetrating peptide (CPP) to a protein of interest thereby giving it...

...of K. phaffii lacks certain terminal mannose glycopeptides (which most other yeast possess) that are problematic...

...the pMinDisplay backbone is fused to a specific peptide segment (PDGFR TMD) that anchors the sensor to ...

... translated, the SunTag sites on the nascent polypeptide recruit scFV-GFP, generating green puncta that...

...protein. Animals are first injected with a small peptide that represents a short, unique amino acid sequence...

... the protein of interest by a 2A self-cleaving peptide (Goedhart et al, 2010). An explanation of the assay...

...any exogenous sequences and regenerates a native peptide bond at the split site. Figure 1: The base...

...want to generate antibodies against, or a unique peptide sequence derived from this protein. At the same...

...are secreted from cells due to their IgGκ signal peptide sequence, making them easy to harvest from cell...

...homodimerization tendency. This assay employs a peptide derived from cytochrome p450, CytERM, to direct...

...protein synthesis: incorporation of puromycin into peptide chains." Proceedings of the National Academy of...