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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 119 results for: PEP

Showing: 41 - 60 of 119 results

  1. Better Dyeing Through Chemistry & Small Molecule Fluorophores

    Type
    Blog Post
    ...short genetically encoded tetracysteine (Cys4) peptide tag could be used to label proteins in cells (Figure...covalent attachment of a fluorophore ligand to a peptide tag (Figure 1c). Click chemistry (e.g., transcyclooctene–tetrazine...

  2. Neuronal labeling with Spaghetti Monster

    Type
    Blog Post
    ...neuronal circuits. Epitope tags are short antigenic peptide sequences, attached to a protein of interest (POI...simian virus 5-derived epitope (V5), the synthetic peptide FLAG, the synthetic streptavidin-binding strep-...

  3. Hot Biosensors 2022: Year-End Roundup

    Type
    Blog Post
    ...Addgene’s catalog is a new class of sensors for neuropeptides and neurotransmitters. Motivated to develop ...GPCRs recognizing various neurotransmitters, neuropeptides and other ligands - almost A to Z! - from ATP...

  4. Antibodies 101: Single Chain Fragment Variables (scFvs)

    Type
    Blog Post
    ...recombinant antibody. They are ~25 kDa single polypeptides that contain the variable light chain (VL) and... two chains are connected by a flexible linker peptide that is usually 15-20 amino acids long and made...

  5. Using Phosphoserine to Study Protein Phosphorylation

    Type
    Blog Post
    ...extension and translation of the pSer-incorporated polypeptide. These three components were shown to effectively...proportional to a single phosphate moiety on the peptide of interest.  After confirming pSer incorporation...

  6. Viral Vectors 101: AAV Serotypes and Tissue Tropism

    Type
    Blog Post
    ...Laminin receptor (LR)3 AAV receptor (AAVR)2 Carboxypeptidase D (CPD), also called AAVR25   AAV9 Terminal...receptor (LR)3 Putative integrin3 AAV11 Carboxypeptidase D (CPD), also called AAVR25   1Wu, Miller...

  7. 27 Hot Plasmids from 2016

    Type
    Blog Post
    .... Mark Howarth’s lab recently engineered a new peptide/protein pair, SnoopTag/SnoopCatcher, that can be...link desired proteins together via a spontaneous isopeptide bond. When used in combination with their previously...

  9. Antibodies 101: Affinity Tags

    Type
    Blog Post
    ...This is where affinity tags come in – they are peptides which do not naturally exist in most expression...

  11. Another Pathway into Cells: iTOP

    Type
    Blog Post
    ...transduction strategy is to fuse a cell penetrating peptide (CPP) to a protein of interest thereby giving it...

  12. K. phaffii: Rising to the Occasion

    Type
    Blog Post
    ...of K. phaffii lacks certain terminal mannose glycopeptides (which most other yeast possess) that are problematic...
Showing: 41 - 60 of 119 results