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We narrowed to 741 results for: REV

Showing: 41 - 60 of 741 results

  1. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

    Type
    Blog Post
    ...genome. The packaging plasmid contains Gag, Pol, Rev, and Tat genes. Specifically for our knowledge here...integration, and how researchers have modified them to prevent this integration.  Lentiviruses evolved to integrate... continue. They rely on a viral protein called reverse transcriptase to convert their RNA genomes into...viral genome is then part of the cellular DNA… forever!  This can be an advantage for researchers looking...integrase-deficient lentiviral vectors, let’s briefly revisit the basic biology of lentiviral vectors (for a ...processed from the larger protein: PR (protease), RT (reverse transcriptase), and IN (integrase). These viral... viral life cycle initially proceeds as usual. Reverse transcription of the transgene occurs in the cytoplasm...

  2. What is Polymerase Chain Reaction (PCR)

    Type
    Protocol
    ...PCR tubes. Put 50µl – 2.5µl (fwd primer) – 2.5µl (rev primer) = 45µl volume of master mix to add to each...primers for 30 seconds at 55°C: The forward and reverse primers are stable within this temperature range...nt) 2.5 μL Forward Primer (10 μM stock) 2.5 μL Reverse Primer (10 μM stock) 36.8 μL Sterile dH 2 O 0.2...nt) 2.5 μL Forward Primer (10 μM stock) 2.5 μL Reverse Primer (10 μM stock) 0.2 μL Taq DNA Polymerase ...DNA can be added to the master mix. Forward and Reverse Primers DO NOT get added to a master mix. Place...several times. Keep tube on ice. Add the forward and reverse primers to the thinned walled PCR tubes. Note: ... primer onto one side of the tube wall and the reverse primer onto the other. Add the master mix to the...

  3. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

    Type
    Blog Post
    ...–Cas in agriculture and plant biotechnology. Nat Rev Mol Cell Biol 21:661–677 . https://doi.org/10.1038...cytosine base editor or adenine base editor) and reverse transcriptase-mediated prime editing technologies...precise edits tend to be more efficient than HDR (reviewed in Zhu et al., 2019). Both cytosine base editors...Lew TTS, Sweeney CJ, Koman VB, Wong MH, Bohmert-Tatarev K, Snell KD, Seo JS, Chua N-H, Strano MS (2019)...

  4. Plasmids 101: Using Transposons in the Lab

    Type
    Blog Post
    ...Eukaryotic Genomes. Annu Rev Genet 41:331–368. https://doi.org/10.1146/annurev.genet.40.110405.090448 Kumar...transcripts and then rely on a reverse transcriptase enzyme to reverse transcribe back into DNA before...another. This protein is either a transposase or a reverse transcriptase, depending on the mechanism of transposition...: gag and pol. The pol polyprotein encodes the reverse transcriptase and integrase enzymes which are required... with a poly(A). ORF2 encodes endonuclease and reverse transcriptase activities.    Figure 1: ...themselves as RNA transcripts and then use their reverse transcriptase (RT) enzyme to convert back into ...need to move around, either a transposase or a reverse transcriptase. Non-autonomous transposons, however...

  5. RNA Interference in Plant Biology: New Tools for an Old Favorite

    Type
    Blog Post
    ...RNA Interference. Annu Rev Biophys 42:217–239 . https://doi.org/10.1146/annurev-biophys-083012-130404 ...downstream of DNA, such as RNA interference, can reversibly exert their effect independent of gene copy number...techniques It is fair to say that CRISPR has revolutionized molecular biology and has enabled functional...same gene. However, recent work has called this prevalent assumption into question. Specifically, CRISPR-generated...DNA template for the RNAi trigger is the most prevalent method due to ease of use and capacity to generate...RNA Interference System in Physcomitrella patens Reveals a Dominant Role of Augmin in Phragmoplast Microtubule...

  6. AAV Titration by qPCR Using SYBR Green Technology

    Type
    Protocol
    ... 2012 ) fwd ITR primer, 5'-GGAACCCCTAGTGATGGAGTT rev ITR primer, 5'-CGGCCTCAGTGAGCGA ITR-containing plasmid...water, then SYBR master mix, then the forward and reverse primers. Vortex briefly, immediately before use... μL 100 μM Forward Primer 0.15 μL 15 μL 100 μM Reverse Primer 0.15 μL 15 μL Nuclease Free Water 4.7 μL... made in step #1): Volume of 2 x 10 9 stock or previous dilution (μL) Volume of nuclease-free water (uL...

  9. Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins

    Type
    Blog Post
    ... and mechanobiology: seeing is believing." Annu. Rev. Biomed. Eng. 10 (2008): 1-38. PubMed PMID:18647110... 570/ 596 Reversible photoactivation In contrast to irreversible PA-FPs, reversible PA-FPs (also known... into 2 main categories - those with irreversible and reversible photoactivation - and have enabled sophisticated...sophisticated imaging techniques.     Irreversible photoactivation Irreversible PA-FPs can be switched on from...another group of reversible PA-FPs. These were engineered to be the first reversible, monomeric red fluorescent...their fluorescence can be switched on and off reversibly. This ability to modulate fluorescence allows...takes less than a second. The first successful irreversible PA-FP reported was PA-GFP. PA-GFP was derived...

  10. Lentivirus ddPCR Titration

    Type
    Protocol
    ...primers and probes targeting integrated copies of the Rev response element (RRE) in the target cells but can...tgtgccttggaatgctagt probe (FAM): tttggaatcacacgacct reverse primer: aatttctctgtcccactccatc PrimePCR ddPCR Copy...

  11. Lentiviral Vector Guide

    Type
    Guide
    ... containing reverse transcriptase and integrase components. rev in trans Binds to the Rev Response Element... , pol , tat , and rev Two plasmids, one encoding gag/pol and another encoding rev Envelope Plasmid Interchangeable...nuclear export. RRE in cis Rev Response Element; the sequence to which the Rev protein binds. Psi (Ѱ) in...additionally require the regulatory genes tat and rev , along with virus-specific accessory genes (for ...genes gag and pol , and regulatory genes tat and rev ; separated into two plasmids in third generation...Packaging plasmid — contains gag , pol , tat , and rev Envelope plasmid — contains env All second-generation...contains gag and pol Packaging plasmid 2 — contains rev Envelope plasmid — contains env Third-generation ...

  12. Validated gRNA Sequences

    Type
    Collection
    ... cerevisiae CTAGATATTAAAATGTCTAA 64379 activate S. pyogenes 23977949 Lu CYC1m promoter S. cerevisiae GTTGAAAGTATTAGTTAAAG...70655 cut S. pyogenes 26472758 Sabatini CAN1 S. cerevisiae GATACGTTCTCTATGGAGGA 43803 cut S. pyogenes 23460208...interfere S. pyogenes 23849981 Qi CYC1m promoter S. cerevisiae ACAGAGCACATGCATGCCAT 64385 activate S. pyogenes...pyogenes 23977949 Lu CYC1m promoter S. cerevisiae ACTAATACTTTCAACATTTT 64387 activate S. pyogenes 23977949...23977949 Lu CYC1m promoter S. cerevisiae ATATCGAATTCCTGCAGCCC 64382 activate S. pyogenes 23977949 Lu CYC1m promoter... promoter S. cerevisiae ATATTCTTTCCTTATACATT 64380 activate S. pyogenes 23977949 Lu CYC1m promoter S. ...activate S. pyogenes 23977949 Lu CYC1m promoter S. cerevisiae TACATACAGTAGGATCCTA 64381 activate S. pyogenes...

  13. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...Khakh ATP FRET-based ATP sensor ATeam1.03-nD/nA Reversible dimerization of Aequorea victoria fluorescent...patterning of cGMP in vascular smooth muscle cells revealed by single GFP-linked biosensors. Proc Natl Acad...Peredox-mCherry in plants In Vivo NADH/NAD+ Biosensing Reveals the Dynamics of Cytosolic Redox Metabolism in Plants...for pyruvate A highly responsive pyruvate sensor reveals pathway-regulatory role of the mitochondrial pyruvate...pHluorin Two pathways of synaptic vesicle retrieval revealed by single-vesicle imaging. Neuron. 2009 Feb 12...macropinocytosis by lowering submembranous pH and preventing Rac1 and Cdc42 signaling. J Cell Biol. 2010 Feb...for pH sensing The zebrafish merovingian mutant reveals a role for pH regulation in hair cell toxicity ...

  14. Brain Initiative Collection

    Type
    Collection
    ...pAAV-EF1a-iCreV can be used to generate AAV virus that will express light-inducible site-specific iCreV recombinase...pAAV-EF1a-iDreV can be used to generate AAV virus that will expresslight-inducible site-specific iDreV recombinase...Neurotechnologies® (BRAIN) Initiative aims to revolutionize our knowledge of the human brain through the... behavior. Long desired by researchers, the revolutionary dynamic picture of the brain generated through...will provide novel ways to treat, cure, and even prevent brain disorders. The BRAIN Initiative supports ...

  15. Open Enzyme Collection

    Type
    Collection
    ...Ligases DNA Polymerases Restriction Endonucleases Reverse Transcriptases RNA Polymerases Other Enzymes You...DNA polymerases, RNA polymerases, DNA ligases, reverse transcriptases, and restriction enzymes. These ...reaction (PCR), cloning, isothermal amplification, reverse transcription of RNA into DNA, and dNTP synthesis...Ligases DNA Polymerases Restriction Endonucleases Reverse Transcriptases RNA Polymerases Other Enzymes DNA...pOpen-XbaIR XbaI 165568 pOpen-NcoIR NcoI Return to top Reverse Transcriptases Addgene ID Plasmid Gene/insert 165546...pOpen-MMLV_RT (mut H) Moloney Murine Leukemia Virus (MMLV) Reverse Transcriptase (RNAse H deactivated by 3 mutations... (lack H) Moloney Murine Leukemia Virus (MMLV) Reverse Transcriptase RNaseH - (lacking RNaseH domain) ...

  16. CRISPR Guide

    Type
    Collection
    ...the donor template, incorporating mutations that prevent further Cas9 cleavage will improve the accuracy... method in which Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) is fused to the C-terminus...the desired edit is incorporated into the DNA by reverse transcription. The edited DNA strand displaces ...editing. PASTE uses a CRISPR-Cas9n fused to both a reverse-transcriptase and a serine integrase (Bxb1), along...dCas9-mediated gene activation or repression is reversible, as it does not permanently modify the genomic...multiple fusions. The effects of CRISPRi/a may be reversible once the effector is inactivated or removed from... and even cell differentiation. The system is reversible, with a companion CRISPRon to undo repression...

  17. CRISPR Plasmids - Prime Edit

    Type
    Collection
    ...search and replace” gene editing method in which a reverse transcriptase (RT) is fused to the C terminus of...the desired edit is incorporated into the DNA by reverse transcription. The edited DNA strand displaces ... use the Moloney murine leukemia virus (M-MLV) reverse transcriptase. Engineering of the PE enzyme and...editing, including exploring new sources for the reverse transcriptase. Many PE enzymes and related tools...additional protection added to 3’ tail of pegRNA to prevent RNA degradation For more in-depth information on...Design Tools CRISPR Blogs CasPEDIA Content last reviewed: 17 October 2025 Do you have suggestions for other...

  18. Caltech Systemic Capsids

    Type
    Collection
    ...140135 pAAV-EF1a-iCreV EF1a light-inducible recombinase iCreV Zeng 140136 pAAV-EF1a-iDreV EF1a light-inducible...strains. **AAV1-X1 has not been tested in rats. Abbreviations: AAV, adeno-associated virus; GRE, gene regulatory...check out Addgene's blog on Systemic Capsids . To review performance data of each of these serotypes, please...light-inducible recombinase iDreV Zeng 140137 pAAV-EF1a-iFlpV EF1a light-inducible recombinase iFlpV Zeng 164450...

  19. Tetracycline Inducible Expression

    Type
    Collection
    ...most popular choices for rapid, efficient, and reversible control of gene expression in eukaryotes. To ...VP16 transcriptional activation domain); rtTA: reverse tetracycline-controlled transactivator (fusion ...tetracycline-dependent repression. Mutating these residues led to a reverse Tet repressor, or rTetR, which specifically binds...the TRE in the presence of tetracycline. A new reverse tetracycline-controlled transactivator (rtTA) was...TetR-tetO system, although the DNA-binding profile is reversed (Figure 1). However, rtTA can induce stronger ... marker. Aviv Regev , Joan Brugge Content last reviewed 20 June 2025. References Das, A. T., Tenenbaum...

  20. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ... vectors - PCR-based C-terminal tagging in S. cerevisiae pCS2FLAG - N- or C-terminal 2xFLAG...of proteins on the extracellular surface of S. cerevisiae cells His-tagged versions of pFastBac LIC vectors...Plasmids page pBF3060 - Metabolic engineering in S. cerevisiae TALENs Gene targeting TALEN kits - Construct ... and expression of bacterial gene clusters S. cerevisiae plasmids for multiple marker selection TRP1 Yeast...InvivoGen trademark. Return to top Content last reviewed 28 May 2025. Do you have suggestions for other...
Showing: 41 - 60 of 741 results