We narrowed to 844 results for: Tat

...Furthermore, in cells containing two mutated alleles, each mutated allele may be different owing to the...many different types of edits, including point mutations, base changes, and large insertions or deletions...increases specificity but is less efficient Each putative knockout allele must be experimentally verified...) Generate specific genomic edits, like point mutations or small tag insertions, by copying from a DNA...Generate targeted insertions, deletions, and point mutations using a prime editing guide RNA (pegRNA) Prime... alternative splicing, and an early frameshift mutation is more likely to result in a non-functional protein...as there are for viral vectors — the only size limitation is what you can efficiently transfect. However...

...frequently mutated gene (>50%) in human cancer; most of these mutations are missense mutations in the DNA-binding...regulator; also known as MDM4 Noxa Phorbol-12-myristate-13-acetate-induced protein 1; also known as PMAIP1 ...19776742 . Germline TP53 mutations and Li-Fraumeni syndrome. Varley JM. Hum Mutat. 2003 Mar;21(3):313-20..., often at one of six hotspot residues. These mutations can be inherited or arise through exposure to ... (e.g., HPV, the human papillomavirus). These mutations interfere with p53’s ability to activate transcription...Li-Fraumeni syndrome. Recently some impacts of p53 mutations have been characterized as gain-of-function, expanding...;415(6867):45-53. PubMed PMID: 11780111 . p53 mutations in human cancers. Hollstein M, Sidransky D, Vogelstein...

...The packaging plasmid contains Gag, Pol, Rev, and Tat genes. Specifically for our knowledge here, the Pol...by long terminal repeat (LTR) sequences, which facilitate integration of this portion into the host genome...vesicular stomatitis virus G protein (VSV-G), which facilitates viral entry into the host cell.  Transfecting...(see Figure 2).  Figure 2: Schematic representation of integration process and episomal forms of...Addgene: psPAX2-D64V. This construct has the D64V mutation, a single amino acid change (from aspartic acid...detecting vector particle components or performing quantitative PCR titration of vector DNA in samples harvested...Insertional mutagenesis combined with acquired somatic mutations causes leukemogenesis following gene therapy of...

...protein complexes. Includes: Immunoprecipitation (IP) Chromatin immunoprecipitation (ChIP) Visualization - ...Capture methods such as immunoprecipitation (IP) and chromatin immunoprecipitation (ChIP) allow you to isolate...which emit a specific wavelength of light after excitation by light of a specific wavelength. While more...interactions in a specific timeframe or context. Immunoprecipitation (IP) IP uses immobilized antibodies to isolate... on the same general strategy. Figure 5: Immunoprecipitation using bead-based antibody capture to isolate...isolating protein from serums. Chromatin immunoprecipitation (ChIP) This variation of IP uses antibodies...for individual assays. Clear cell debris by sedimentation. Proceed with IP using a primary antibody for...

..., the fluorescence lifetime (the time between excitation of the fluorophore and emission of a photon) ...-ShadowY , EGFP-ShadowY λ Dex : Donor maximum excitation wavelength (nm), QY D : Donor quantum yield, ...Overlap integral of the donor emission and acceptor excitation spectra (×10 15 M -1 cm -1 nm 4 ). Values obtained...organism or cell type, relative position and orientation of the fluorophores through linkers, fluorescent...linkers of varying length Evers et al. (2006) Quantitative understanding of the energy transfer between...FRET efficiency standards Thaler et al. (2005) Quantitative multiphoton spectral imaging and its use for...stoichiometry of protein interactions using image-based quantitative two-hybrid FRET Positive and negative controls...

... purified through affinity chromatography. To facilitate the purification process, proteins are often ... reduces bacterial protein contamination and facilitates extraction. Browse our most popular plasmids ...transfer (FRET) and bimolecular fluorescence complementation (BiFC) are techniques that have been widely...fusing each of them to two FPs whose emission and excitation spectra overlap. That way if your proteins of...compatible vectors for bimolecular fluorescence complementation experiments in plants. The Zinc Finger Consortium...Introduction to FRET Bimolecular Fluorescence Complementation Makes Visualizing Protein–Protein Interactions...study the effects of protein expression in the stationary phase. In these cases, you may want to control...

...that can hold larger containers, but they can also rotate at much higher speeds and are used for more specialized...balanced. Using the centrifuge in an unbalanced state can damage the centrifuge and be dangerous for the...

...host organism. Host Relevant Promoters Representative Empty Backbones Mammalian CMV, SV40, EF1a, ...could allow you to conduct western blots or immunoprecipitations of your favorite gene even if you do...Tag or Fusion Protein Common uses Representative Empty Backbones Flag Epitope tag...methods. Delivery method Advantages Representative Empty Backbones AAV High transduction...expression, transgenics, and more. Element Details Representative Empty Backbones CRISPR Genome modification... Marker Typical Host Organism Representative Empty Backbones Neomycin (G418) ...Gateway vectors) pCAP03-acc(3)IV - Facilitate the capture and expression of bacterial gene ...

...correcting a typo in the latest draft of your dissertation. Well, that will probably never happen. Yet ... to this DNA targeting ideal. Figure 1: (A) Representation of a zinc finger (ZF) nuclease. Zinc fingers...for molecular tools. ZFs are not without their limitations, however. Not every nucleotide triplet has a ...Martin-Luther-University Halle-Wittenberg and Adam Bogdanove at Iowa State University published the nucleotide recognition ...if not slightly lower. Figure 2: Simplified representation of the Voytas/Bogdanove Golden Gate TALEN kit...in a C. elegans lab and have been struggling to mutate a specific gene or your anxiously investigating...

...contact with hazardous materials. A sink, eyewash station, safety shower, fire blanket, and extinguisher ... are located before you start. Use the eyewash station if unwanted or biohazardous materials are splashed...

...that the insert will be added in the correct orientation and prevents the vector from ligating to itself...indicates the various controls: Control Ligase Interpretation Uncut vector - Checks viability of competent...

...TALEN Guide. These collections and accompanying documentation allow one to efficiently assemble TALEN constructs...truncated at both the N and C terminus and induces mutation at rates much higher than the parental vectors...generation architecture has been shown to increase mutation induction when using TALENs. The FokI domains ...homodimeric or enhanced heterodimeric (ELD, KKR mutations) FokI domains. 40131 pCAG-T7-TALEN(Sangamo)-FokI-KKR-Destination...are designed to target and edit the epigenome of putative enhancers in mammalian cells. These plasmids allow...suitable for the TALEN-based generation of germline mutations in Bombyx mori and Drosophila melanogaster . Zhang...

...MGC110940, OPN SST somatostatin SMST SSTR1 somatostatin receptor 1 SRIF-2 SSTR2 somatostatin receptor 2 - SSTR5...arginine-rich, mutated in early stage tumors ARP, MANF, MGC142148, MGC142150 ARMETL1 arginine-rich, mutated in early...GHRFR, GHRHRpsv, GRFR GHRL ghrelin/obestatin prepropeptide MTLRP, obestatin GHSR growth hormone secretagogue...growth inhibitor 1 BDGI, OKL38 OSM oncostatin M MGC20461 OSMR oncostatin M receptor MGC150626, MGC150627,...have plasmids available for request. Antigen Presentation The cornerstone of the immune response is the...including dendritic cells and macrophages. Antigen presentation is mediated by the MHC class I and class II ...available for request. Antigen Processing and Presentation The cornerstone of the immune response is the...

...or not, without a guaranteed titer. Any titer expectations communicated by email will supersede the general...after you submit your request, so we can set expectations to the best of our knowledge from the start....not, with a longer turnaround time. Any timing expectations communicated by email will supersede the general...material received. Shipping While Addgene assists importation by including thorough shipping documents and ...taxes, and to complete any additional customs documentation that might be required by your country for the...

...loss-of-function mutations in p53 and Lkb1 , as well as homology directed repair-mediated Kras G12D mutations, leading...harnessed the type II CRISPR nuclease system to facilitate genome editing in mammalian cells (Cong et al...AAV plasmid for sgRNA cloning. GFP-KASH fusion facilitates FACS sorting of cells and nuclei. Return to top...KRAS , p53 and LKB1 , the top three significantly mutated genes in lung adenocarcinoma. Delivery of a single...hSyn-Cre-2A-EGFP-KASH-WPRE-shortPA-ITR This plasmid facilitates Cre/loxP recombination and fluorescence assisted...

...continuously divide while maintaining an undifferentiated state. Stem cells are classified as pluripotent or multipotent...adult somatic cells are returned to a pluripotent state using a cocktail of factors (Oct3/4, Sox2, c-Myc...This “reprogramming” of the cell to an embryonic state holds immense potential for drug development, disease... cell division, all of which lower the risk of mutation and minimize tumorigenicity, an inherent disadvantage...Lentiviral Mouse Direct Induction of the Three Pre-implantation Blastocyst Cell Types from Fibroblasts. Cell...Neurons Lentiviral Human Rapid Ngn2-induction of excitatory neurons from hiPSC-derived neural progenitor ...

...autoclave, you should prepare your plate pouring station: Find an empty section of lab bench with a working...thermometer. Light the flame at the plate pouring station and dilute your antibiotic into your ~60 ℃ molten...

...Firefly Insertion of 5' promoter/enhancer regions. Mutations within the luciferase gene allow for increased...Firefly luciferase fragment for split-luciferase complementation assay in plants using Gateway cloning. Use ...Fifrefly luciferase fragment for split-luciferase complementation assay in plants using Gateway cloning. Use ...deep-learning approaches. Split Luciferase Complementation assays can report on protein-protein interactions... : Constructs for split firefly luciferase complementation assays in plants. Bioluminescence Resonance...

...glyoxysomal, mitochondrial, peroxisomal) Mutation (specific mutation, "active site", "active site loop", or...shared experience for the research community and facilitates scientific collaborations between institutions...interface") ID Plasmid Description Gene/Insert Mutations Additional Resources MDH CUREs Community (Link...

...for 3 h without stirring to allow full precipitation. Precipitation of the viruses can proceed overnight...