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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 501 results for: Trac;

Showing: 41 - 60 of 501 results

  1. Protocol - How to Perform Sequence Analysis

    Type
    Protocol
    ...the trace file, you will see that these base calls are unreliable. This is an example of a trace file...with the sequence, trace file, and primer used. What program can I use to view my trace file? There are ...You should receive your sequencing results as a trace file (.ab1) which graphically depicts the sequence...four nucleotide bases. This is an example of a trace file from a high-quality portion of a sequencing...and include: The sequencing result The original trace file (.ab1 file) provided by your sequencing company...sequencing result, what should I do? Check your trace file first; the apparent mismatch/mutation may be... be the result of a mis-called peak in the trace file. If the mutation is not an artifact, please email...

  2. Brain Initiative Collection

    Type
    Collection
    ...pAAV-syn-FLEX-splitTVA-EGFP-tTA helper virus for monosynaptic tracing; to be coinjected with pAAV-TREtight-mTagBFP2-B19G...pAAV-TREtight-mTagBFP2-B19G helper virus for monosynaptic tracing; to be coinjected with pAAV-syn-FLEX-splitTVA-EGFP-tTA...

  3. Protocols for Molecular Biology, Plasmid Cloning, and Viral Preps

    Type
    Protocol
    ...features using sequence analysis RNA Extraction Without a Kit Extract RNA from tissues or cells without ...! DNA Purification Miniprep, phenol-chloroform extract, and precipitate DNA DNA Quantification Measure...choice AAV Purification by Iodixanol Gradient Ultracentrifugation Purify adeno-associated virus from a preparation...

  4. Handling Plasmids from Addgene - Purifying Plasmid DNA

    Type
    Protocol
    ...phenol-chloroform extraction - see protocol below . Note: Phenol-chloroform extraction removes remaining...-50 μL of TE. Top Protocol: Phenol-Chloroform Extraction of DNA Samples Add an equal volume of TE-saturated...it is advisable to perform a phenol/chloroform extraction of the supernatant after step 6 and before step...

  5. Protocol - pLKO.1 – TRC Cloning Vector

    Type
    Protocol
    ...generation of shRNA transcripts. cPPT Central polypurine tract, cPPT, improves transduction efficiency by facilitating...competent cells Invitrogen #18258-012 Qiaquick gel extraction kit Qiagen #28704 Low melting point agarose Sigma...at 37°C for 2 hours. Purify with Qiaquick gel extraction kit. Elute in 30 μL of ddH 2 O. Digest eluate... the DNA. Purify the DNA using a Qiaquick gel extraction kit. Elute in 30 μL of ddH 2 O. Measure the DNA...AB01200-02000, LB contains: 10g tryptone 5g yeast extract 10g sodium chloride 15g agar Prepare LB agar solution...

  6. MAPK Plasmids

    Type
    Collection
    ...Mitogen-activated protein kinases (MAPKs), also known as extracellular signal-regulated kinases (ERKs), are serine/...

  9. Fluorescent Protein Guide

    Type
    Collection
    ...Monitor small biomolecules or other physiological intracellular processes with genetically encoded fluorescent...

  10. DNA Service - Cloning Grade DNA

    Type
    Collection
    ...experiments by removing the amplification and extraction steps required when one receives plasmids in ...

  11. All Antibodies

    Type
    Collection
    ... other difficult-to-target antigens that are intractable via animal immunization. Addgene's antibody catalog...

  12. Institute for Protein Innovation

    Type
    Collection
    ... other difficult-to-target antigens that are intractable via animal immunization. IPI is a nonprofit research...

  13. Optogenetics Plasmid Collection

    Type
    Collection
    ... a fluorescent protein to the opsin in order to track its expression and localization. Many other types...

  15. Synthetic Biology - Overview

    Type
    Collection
    ...Synthetic Biology Articles - Peccoud lab social media tracker Synthetic Biology - Open access journal Do you ...

  16. Retrovirus Plasmids

    Type
    Collection
    ...resistance. Hahn 63704 pRetroX GFP T2A Cre CMV/MSV Tetracycline inducible expression of GFP T2A Cre fusion in...

  17. Worm Expression Resources

    Type
    Collection
    ...consists of 959 somatic cells, which makes them attractive for imaging and fluorescence microscopy experiments...

  18. AAV Packaged on Request

    Type
    Collection
    ...experiments. Shipping 2–7 days We will email you with tracking information as soon as your order ships. Shipping...

  19. Arf GTPase Family

    Type
    Collection
    ...Spang, A., Dacks, J. B., & Ettema, T. J. (2016). Tracing the archaeal origins of Eukaryotic Membrane-Trafficking...
Showing: 41 - 60 of 501 results