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Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 1,030 results for: URE

Showing: 41 - 60 of 1030 results

  1. p53 Pathway

    Type
    Collection
    ...These mutations can be inherited or arise through exposure to mutagens like radiation or viruses (e.g., HPV...Thompson T, Karsenty G, Bradley A, Donehower LA. Nature. 2002 Jan 3;415(6867):45-53. PubMed PMID: 11780111...Ogryzko V, Gardner K, Giordano A, Levine AS, Kelly K. Nature. 1997 Jun 27;89(7):1175-84. PubMed PMID: 9215639...

  2. Fluorescent Protein Guide: FRET

    Type
    Collection
    ...collection of plasmids suitable for FRET experiments to measure protein interactions or conformation changes, as...experiments. The efficiency of the energy transfer is measured using fluorescence microscopy by exciting the ...calibrate the numerous and disparate methods used to measure FRET. Plasmid FRET Pair C5V Cerulean attached to...

  3. Viral Vectors

    Type
    Collection
    ...genetic material to specific cells in an organism. Figure 1. Using viral plasmids to generate virus. A) Viral...host and giving rise to more virus (as happens in nature), researchers can introduce plasmids to a host ...required for viral replication. Thus, as a safety feature, viruses used in research typically do not have...

  4. CRISPR Plasmids - Tagging

    Type
    Collection
    ...gene knock-in. The detailed procedure can be found in Sakuma et al., Nature Protocols, 2016 . Yamamoto ...very rapidly after the addition of auxin to the culture medium. Plasmids can be found associated with the...

  5. Luciferase Plasmid Collection

    Type
    Collection
    ...plasmid and the effects on gene expression can be measured by the luminescence output. The most popular types...substrate must be delivered intracellularly to measure luminescence. Gaussia luciferase is secreted and...altered emission, substrate use, stability, or other features. AkaLuc : Engineered to use the substrate Akalumine...

  6. Institute for Protein Innovation

    Type
    Collection
    ...and accessible toolset for scientists worldwide. Figure courtesy of IPI Browse Glypican Antibodies ID Recombinant..., blocking ligand binding and receptor function Figure courtesy of IPI Browse Integrin Antibodies ID Recombinant...

  7. CRISPR Plasmids - Cascade-Cas3

    Type
    Collection
    ...found in bacteria and archaea. While abundant in nature, Class 1 systems are largely underutilized compared...many still separate the individual Cas components. Figure 1: Overview of Cascade-Cas3 mechanism. Created ...

  8. Bikard Lab - CRISPR Repression Collection

    Type
    Collection
    ... Biology, 2(9), 537–541. DOI: 10.1021/sb400021j Figure 1: Multiplexing CRISPR knockdown to modulate expression...concentration given relatively to the non‐targeting spacer measured by high‐throughput microscopy for a collection...

  9. Brzezinski Lab CRISPR Collection

    Type
    Collection
    ...regulation and developmental studies. Key plasmid features and pX458 modifications: replacement of the Cbh...for gene activation CRISPR/Cas9 Plasmid Variants Figure 1: Schematic of the CRISPR Collection. Plasmids...

  11. Resolute Plasmid Collection

    Type
    Collection
    ...2020 research and innovation programme and EFPIA. Figure 1: Representation of RESOLUTE mission. The combination...is embedded in an integrated knowledgebase that features, among others, genetic variants and disease associations...

  12. Mammalian RNAi Tools

    Type
    Collection
    ...shRNA design and delivery, see resources below. Figure 1: Overview of shRNA-mediated RNA interference....silence: conditional systems for RNA interference . Nature Methods, 3 (9), 682–688. PubMed (Link opens in ...

  13. New England Biolabs Cell-Imaging Plasmid Collection

    Type
    Collection
    ...for distribution to the research community. Key features: Allows for visualization of multiple proteins...these tags. Plasmids Addgene ID Plasmid Substrate Feature 101137 pSNAP-tag (T7) Vector SNAP-tag Empty backbone...

  14. Distribution to Industry

    Type
    Collection
    ... Distribution to Industry Featured Collections Search Plasmids Plasmids Kits Pooled...Libraries Antibodies Ordering and MTA Information Featured Collections NEW NanoLuc and HaloTag Fusions from...

  15. Arf GTPase Family

    Type
    Collection
    ...GTPase signaling activity. Figure 1: Locations of ARF superfamily members. Figure from Szutl, et al., 2019...

  16. Zebrafish Plasmid Collection

    Type
    Collection
    ...transparency of the embryo and larvae, it is easy to culture large populations that are amenable to genetics...combinatorial and cumulative genome editing - Jay Shendure Lab Simultaneous single-cell profiling of lineages...

  17. CRISPR Plasmids - Mammalian Expression

    Type
    Collection
    ... to immunoprecipitate FLAG-tagged Cas9. In the CAPTURE system, Cas9 is tagged with a biotin acceptor site...streptavidin affinity purification. For both enCHIP and CAPTURE, design your gRNA sequence to direct dCas9 to a...

  18. Lentivirus Plasmids

    Type
    Collection
    ...1, which may require additional lab biosafety procedures. Additionally, because this virus integrates ...Lentiviral Envelope and Packaging Plasmids One safety feature of lentivirus used in research is that the components...

  19. Caltech Systemic Capsids

    Type
    Collection
    ...Center at Caltech and can help guide your decision. Figure 1: Decision tree for choosing your AAV capsid. ...Information When using the Caltech serotypes in future publications, please acknowledge Viviana Gradinaru...

  20. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ...interest to create a luciferase reporter Promoter Measure promoter strength pBV-Luc - Luciferase ... vectors) pCAP03-acc(3)IV - Facilitate the capture and expression of bacterial gene clusters S. cerevisiae...
Showing: 41 - 60 of 1030 results