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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 642 results for: ato

Showing: 41 - 60 of 642 results

  1. TALEN Engineering

    Type
    Collection
    ...Reagents from the Keith Joung laboratory for engineering TAL effectors, including designed TALENs, and..., Nat Biotechnol. 2011 ) TALE transcriptional activators in human cells ( Maeder et al., Nat Methods 2013...dimeric TALEN and monomeric TALE transcriptional activator target sites and to simplify the construction ...interest for potential TALEN or TALE transcriptional activator target sites and then generates individual user-friendly...REAL, REAL-Fast and FLASH TALE Transcriptional Activator Expression Vectors for REAL, REAL-Fast and FLASH...

  2. Deisseroth INTRSECT Collection

    Type
    Collection
    ...fluorophores, excitatory and inhibitory opsins , genetically-encoded calcium indicators, and rabies targeting...INTRSECT (intronic recombinase sites enabling combinatorial targeting) is a synthetic molecular targeting...doubly-specified combination of genetic and/or anatomical-defined parameters, by placing two orthogonal...Flp AND NOT Cre Yes Dual recombinase-dependent: Excitatory Opsins Addgene ID Plasmid Logic Sites and Mutations...window) Cummings KA, Clem RL. 2020. Prefrontal somatostatin interneurons encode fear memory. Nat Neurosci...

  3. Synthetic Biology - Overview

    Type
    Collection
    ...and synthetic regulatory elements, including promoters, terminators, repressors, activators, and more. Also... Lab Repressors , Terminators , Light Signaling , Sigma Factors , Resource Allocator and Orthogonal Switches...

  4. Luciferase Plasmid Collection

    Type
    Collection
    ...made luciferase a popular tool for scientists. Regulatory elements such as promoters, enhancers and untranslated...backbone plasmids into which you can clone your regulatory element or gene of interest into to create a ...Lentiviral expression of firefly luciferase and dTomato. A gene of interest can also be inserted into the...Reporter Constructs Already know what gene or regulatory element that you need a luciferase reporter for...search box to find a construct that contains your regulatory element of interest. ID Plasmid Description Gene...Optimized STARR-seq (Self-Transcribing Active Regulatory Region) : Genome-wide screening of enhancers ...

  5. p53 Pathway

    Type
    Collection
    ..., which disrupt its interaction with negative regulators, increase its stability and DNA binding activity...activity, and allow it to bind transcriptional co-activators and modulate transcription. The list of p53 transcriptional...proto-oncogene, E3 ubiquitin protein ligase MDMX p53 regulator; also known as MDM4 Noxa Phorbol-12-myristate-...peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1 PERP TP53 apoptosis...binding component 3 Reprimo TP53 dependent G2 arrest mediator candidate Scotin Shisa family member 5 Sestrins...

  6. Fluorescent Protein Guide: Empty Backbones

    Type
    Collection
    ... pFA6a-link-tdTomato-SpHis5 - Yeast Expression tdTomato-N1 - Mammalian Expression tdTomato-C1 - Mammalian...- Bacterial Expression tdTomato 554 581 95 4.7 1 h Tandem-dimer pCSCMV:tdTomato - Mammalian Expression...Mammalian Expression tdTomato-pBAD - Bacterial Expression Return to top Red Protein Excitation (nm) Emission...

  7. NETRF

    Type
    Collection
    ...from the NETRF-funded investigators listed below. Depositing Principal Investigators (PIs) PI Institution...Shivdasani Dana-Farber Cancer Institute Epigenetic Regulators of Intestinal Endocrine Cells and Carcinoid Tumors...Tumors Qiao Zhou Harvard University Epigenetic Regulators of Intestinal Endocrine Cells and Carcinoid Tumors...

  8. CRISPR Plasmids - Activate Gene Expression

    Type
    Collection
    ...gRNA sequence to direct the dCas9-activator to promoter or regulatory regions of your gene of interest....Catalytically dead dCas9 fused to a transcriptional activator peptide can increase transcription of a specific...separate gRNA expression plasmid to target the dCas9-activator to your specific locus. Figure 1: Overview of ...

  9. Zhang Lab CRISPR Page

    Type
    Collection
    ...Expresses dCAS9-VP64 activator with 2A GFP 61423 : Expresses the MS2-P65-HSF1 activator helper complex with...screening library CRISPR/Cas9 Synergistic Activation Mediator (SAM) is an engineered protein complex for the...: lentiviral vector encoding the MS2-P65-HSF1 activator helper complex with a 2A Hygro resistance marker...JS, Konermann S, Trevino AE, Scott DA, Inoue A, Matoba S, Zhang Y, Zhang F. Cell . 2013 Sep 12;154(6):...

  10. Synthetic Biology - Networks and Gene Regulation

    Type
    Collection
    ...Examples Include: promoters and terminators repressors and activators logic gates Networks and Gene Regulation...includes both naturally ocurring and synthetic regulatory elements, pre-assembled genetic circuits, and...

  11. Malate Dehydrogenase CUREs Community Collection

    Type
    Collection
    ...support educators interested in integrating MDH-related research into their teaching laboratories. They ...professional development, membership to a local hub of educators teaching MDH CUREs, and opportunities to mentor...

  12. Recombinases AAV Preps

    Type
    Collection
    ...mCherry 1 Hui Yang 107738 pAAV-hSyn-Cre-P2A-dTomato Syn dTomato 1, 2, 5, 8, 9, rg*, PHPeB Rylan Larsen 107787... none 1 Connie Cepko 69916 pAAV.cTNT.iCre cTnT tdTomato 9 William Pu 203842 pAAV-Ef1a-fDIO-eGFP-2A-Cre-WPRE...

  13. Fluorescent Protein Guide: Subcellular Localization

    Type
    Collection
    ...Harrison 37351 pQC membrane TdTomato IX Membranes Palmitoylation tdTomato Connie Cepko 22479 FUmGW Membranes...118737 pBOB-CARMIL2 BH domain-tdTomato Plasma membrane CARMIL2 BH domain tdTomato John Cooper 189771 pLCK-mScarlet3...

  14. Plant Plasmids and Resources

    Type
    Collection
    ...systems such as Arabidopsis, wheat, maize, rice, tomato, and a variety of other species. In addition to... genomes using genome engineering; validated in tomato, tobacco, M. truncatula , wheat, and barley - Daniel...metabolic engineering in plants (including tobacco and potato) - Scott Lenaghan. Joint Modular Cloning (JMC) ...

  15. Arf GTPase Family

    Type
    Collection
    ...ARF family plasmids. ARF family members include regulatory GTPases, GEFs, and GAPs....References The ARF (ADP-ribosylation factor) family of regulatory GTPases have long been characterized as members..., 2016). Though best known for their roles as regulators of membrane traffic, they also play critical ...

  17. Lentivirus Plasmids

    Type
    Collection
    ...LTRs Packaging plasmid — contains packaging and regulatory genes; one plasmid in second-generation, two ... and EGFP co-expression. See plasmid 21374 for dTomato version. Bryan Welm 21916 Tet-pLKO-neo 3rd Inducible...FUW-M2rtTA 3rd Contains reverse tetracycline transactivator. Rudolf Jaenisch 131626 pHAGE-mt-mKeima 3rd...

  18. Neurodegeneration Plasmid Collection

    Type
    Collection
    ... 58112 tdTomato-MAPTau-C-10 MAPT tdTomato CMV Parkinson's, FTD Michael Davidson 58113 tdTomato-MAPTau-...TARDBP GFP CMV ALS Zuoshang Xu 28205 wtTDP43tdTOMATOHA TARDBP HA, tdTomato CAG ALS Zuoshang Xu 28206 TDP43 ...ataxia Richard Possemato 102971 pLKO.1P shFXN_2 FXN Friedreich ataxia Richard Possemato 102975 pLKO.1P...pSLIK-NFLAG-hFUS FUS Flag, His TRE ALS Zissimos Mourelatos 51146 DsRed-p150 217-548 DCTN1 CMV ALS, motor...tdTomato-MAPTau-N-10 MAPT tdTomato CMV Parkinson's, FTD Michael Davidson 58259 pBabe-Neuroserpin SERPINI1 Dementia Joan....1P shSOD1 SOD1 ALS Richard Possemato 103913 HTRA2_PDZ_1 HTRA2 GST tac Parkinson's Sachdev Sidhu 104448...James Trimmer 182922 hSOD1-FLAG SOD1 CMV ALS Philip Eaton 183984 huntingtin:TOC031-A07:C246751 HTT Flag CMV...

  19. Plan Your Experiment

    Type
    Collection
    ...fusion with transcriptional activator (such as dCas9-VP64) Many different activator systems exist, including...requires dCas9 to be fused to transcriptional activators that need to be within a given range of the transcription...

  20. Cell Migration Consortium Plasmids

    Type
    Collection
    ...Cell Migration Consortium and Addgene. Member laboratories of the Cell Migration Consortium have deposited...for cell migration research at Addgene. CMC investigators who have deposited plasmids include: Joan Brugge...
Showing: 41 - 60 of 642 results