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Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 58 results for: ef1;

Showing: 41 - 58 of 58 results

  1. Genetic Code Expansion

    Type
    Collection
    ...4xMmaPylT(UCA)_EF1_MmaPylRS PylRS M. mazei Mammalian TGA Simon Elsaesser 174901 pAS_MmaPylT_EF1_NES-MmaPylRS...pCNP) Bacterial TAG Thomas Huber 174890 pAS_8xMmaPylT_EF1_FLAG-MmaPylRS PylRS M. mazei Mammalian TAG Simon... Simon Elsaesser 174896 pAS_4xMmaPylT(UUA)_EF1_MmaPylRS PylRS M. mazei Mammalian TAA Simon Elsaesser 174899...mazei Mammalian TAG Simon Elsaesser 174902 pAS_MmaPylT_EF1_NES-MmaPylRS AF PylRS (Y306A, Y384F) M. mazei...E. coli Bacterial TAG Abhishek Chatterjee 220800 EF1a-L274GMmMetRS-T2A-mCherry MetRS M. musculus azidonorleucine...

  2. Structural Genomics Consortium Plasmids

    Type
    Collection
    ...vector 26117 pNIC-CH EF199843 Hexahistidine tag, AmpR 26103 pNIC28-Bsa4 EF198106 Hexahistidine tag with...with TEV cleavage, KanR 26105 pNIC-CTHF EF199844 Hexahistidine tag, FLAG tag with TEV cleavage, KanR 26106... Z-basic, TEV cleavage, AmpR 26108 pFB-LIC-Bse EF199842 Hexahistidine tag with TEV cleavage, AmpR 26095...

  3. AAV Molecular Tools

    Type
    Collection
    ...axon terminals. 1 Luo 60658 pAAV-EF1α-F-FLEX-mNaChBac-T2A-tdTomato EF1a-driven, Flp-dependent Flp-dependent...System Activity Serotype PI 98747 pAAV-FLEX-EGFPL10a EF1a-driven and Cre-dependent EGFP-tagged ribosomal L10a...ablation. 2 Jessell , Azim 45580 pAAV-flex-taCasp3-TEVp EF1a-driven, Cre-dependent Cre-dependent, bicistronic...

  4. Tetracycline Inducible Expression

    Type
    Collection
    ...Wei Xu 26803 pEnt L1L3 EF1a-tTA-2 Gateway entry vector to express tTA from EF1α promoter, for Tet-Off....transactivator Tet-On 3G rtTA Elena Cattaneo 104543 PB-EF1a-TetOn3G PiggyBac vector expressing Tet-On 3G transactivator...transactivator constitutively expressed by EF1a promoter Tet-On 3G rtTA David Vereide 120309 pAAV-FAH-rtTA3G.... Adam Karpf 167935 pLenti-tetON-KRAB-dCas9-DHFR-EF1a-TagRFP-2A-tet3G Tet-inducible expression of KRAB-dCas9...

  5. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ...Representative Empty Backbones Mammalian CMV, SV40, EF1a, CAG, Ubc Transient expression - Gradia Lab plasmids...Resources collection page Zebrafish CMV, h2afv, XlEef1a1 See our dedicated Zebrafish Plasmids and Resources...backbone with MS2 loops at tetraloop and stemloop 2 and EF1a-zeo resistance marker pLenti CMV/TO GFP-Zeo DEST...

  6. Botman-Teusink Yeast FP Collection

    Type
    Collection
    ...p415 plasmid (Loqué et al., 2007) containing the TEF1 promoter and the LEU2 marker, or the pCEV-G2-Km ...Km plasmid (Vickers et al., 2013) containing the TEF1 or PGK1 promoters and the KanMX marker. ID Plasmid...

  7. Viral Production

    Type
    Collection
    ...: AAV Pro cells were transduced with either pAAV-Ef1a-mCherry-IRES-Cre (55634-AAVrg) alone at 1.7E6 viral...Cre. mCherry expression alone was detected. pAAV-Ef1a-mCherry-IRES-Cre was a gift from Karl Deisseroth...

  8. TALEN Plasmids and Kits

    Type
    Collection
    ...galactose-inducible expression. pTAL6-BB contains the TEF1 promoter, giving constitutive expression of TALORs...gene expression. The vectors listed below have the EF1α promoter for efficient mammalian cell expression...

  9. CRISPR Plasmids - Empty gRNA Vectors

    Type
    Collection
    ...Mammalian Gibson none S. pyogenes Zack pHL-H1-ccdB-mEF1a-RiH 60601 Mammalian BamHI/EcoRI none S. pyogenes...Lentiviral BsmBI yes, cut S. pyogenes Jacks pU6-sgRNA EF1Alpha-puro-T2A-BFP 60955 Mammalian/Lentiviral none S...

  10. Brzezinski Lab CRISPR Collection

    Type
    Collection
    ...modifications: replacement of the Cbh promoter with EF1alpha addition of an mCherry reporter variant nuclear...

  11. Lentiviral Prep Service

    Type
    Collection
    ...encoding dCAS9-VP64 with 2A Blast resistance marker (EF1a-NLS-dCas9(N863)-VP64-2A-Blast-WPRE) Zhang Pooled...

  12. Arf GTPase Family

    Type
    Collection
    ...Bacterial (pGEX4T), Mammalian (pEGFP-N3), Gateway GEF Arfgef1 (BIG1, ARFGEP1) 10565 1849 Mammalian (pcDNA4c, ...

  13. Ras Pathway

    Type
    Collection
    ...guanine nucleotide dissociation stimulator RAPGEF RAPGEF1 RAPGEF2 Rap guanine nucleotide exchange factor ...

  14. Immunology Research Plasmids and Resources

    Type
    Collection
    ...Dfy, FY, GPD, GpFy, WBCQ1 DEFA1 defensin, alpha 1 DEF1, DEFA2, HNP-1, HP-1, MGC138393, MRS DEFA3 defensin...phosphoribosyltransferase 1110035O14Rik, DKFZp666B131, MGC117256, PBEF, PBEF1, VF, VISFATIN NDP Norrie disease (pseudoglioma) ...

  15. Zhang Lab CRISPR Page

    Type
    Collection
    ...backbone with MS2 loops at tetraloop and stemloop 2 and EF1a-zeo resistance marker. Contains BsmBI sites for ...

  16. Promoters

    Type
    Guide
    ...Strong mammalian promoter from human cytomegalovirus EF1a Constituitve Strong mammalian promoter from human...

  17. Chemogenetics Guide

    Type
    Guide
    ...Neurons GFAP Glia CD68 Microglia Dlx Interneurons EF1a, CAG General expression Targeted expression. Depending...

  18. Validated gRNA Sequences

    Type
    Collection
    ...TTGATATTTAAGTTAATAAA 62320 scaffold S. pyogenes 25533786 Qi & Lim TEF1 S. cerevisiae TTGATATTTAAGTTAATAAA 46922 interfere...
Showing: 41 - 58 of 58 results