...What’s a science writer?” Learning about SciComm by doing SciComm Writing had always been a strength of ...right place at the right time when she started her scicomm career: “ I think living in Washington D.C. helped... scicomm...

... Cuneate tract, dCST= dorsal cortico spinal tract, vCST= ventral cortico spinal tract, dc= decussation... first segments of the spinal cord showing the cortico spinal tract, CST, spinal cord and cranial nerve...device (EMCCD) or complementary metal-oxide semiconductor (CMOS) sensors, it takes only a few milliseconds...

...flanking the editing site on either side of the amplicon. Based on the frequency of out-of-frame edits...deleted accordingly. Pro tip: make sure your PCR amplicon isn’t too large so that you can easily visualize...expect off target editing to occur. Luckily, in silico prediction analyses like CRISPRitz or CRISPOR can...CRISPRitz: rapid, high-throughput and variant-aware in silico off-target site identification for CRISPR genome...

... targeted, specific modification of monocot and dicot genomes using genome engineering; validated in tomato...backbones, and promoters for expression in monocots and dicots - Vladimir Nekrasov. The MoClo-compatible zCas9i...high-efficiency multiplex genome editing in monocot and dicot plants - Yao-Guang Liu. Plasmids for prime editing...

...ribosome and if the anticodon on the tRNA can bind to the mRNA (hence, the anticodon is complementary to... variant inactivated by the truncation of the anticodon loop). Expresses archaeal MjTyrRS/tRNA pair instead...

... written jointly by Addgenies Brook Pyhtila and Nicole Waxmonsky Resource sharing shortens the time needed...This post was written jointly by Brook Pyhtila and Nicole Waxmonsky. References McDade J, Waxmonsky NC. Emerging... Nicole Waxmonsky...

...Massachusetts Medical School developed a dCas9-based multicolor labeling system to visualize chromosomal loci... U2OS cells.   Figure 2: CRISPR-Sirius tricolor labeling. (A) Diagram of sgRNA-Sirius-8XMS2, sgRNA-Sirius...plasmids at Addgene. References Ma, Hanhui et al. “Multicolor CRISPR labeling of chromosomal loci in human ...

...little more this October. Science favorites Musicophilia: Tales of Music and the Brain by Oliver Sacks...properties it has. As with other works of Dr. Sacks, Musicophilia includes case studies of relevant neurological...book! Far from a work of glib pop psychology, Musicophilia is accessible and fascinating, all without compromising...

...BioRender.com.   SLIC also opened up a new world of multicomponent assembly, as fragments with overlapping sequence...efficiency (~20%). Figure 2: Schematic of multicomponent assembly with SLIC. Multiple fragments are ...issue is sequence similarity. If fragments in a multicomponent assembly have 5′ or 3′ sequence homology to...

...cell have the same replicon and/or partitioning system. Let’s start with the replicon- the part of the ...The replication problem Plasmids with the same replicon are incompatible because they compete for the ...

...natural Cas orthologs include Hsp1Cas9 (from Helicobacter sp. MIT 11-5569 ) and Nme2Cas9 (from Neisseria...paralogs or lncRNAs . Guide RNAs are designed in silico and synthesized (Figure 8A), then cloned in a pooled...fluorescently-tagged RNA-binding proteins (RBPs). Multicolor CRISPR imaging can be achieved by using orthogonal...Streptococcus thermophilus (ST) 3' NNAGAAW Treponema denticola (TD) 3' NAAAAC Additional Cas9s from various species...Wolfe, S. A., Zhang, S., & Pederson, T. (2015). Multicolor CRISPR labeling of chromosomal loci in human ...

...These probes can be combined in two-color and multicolor NIR imaging.  Small miRFPnano A separate class...monomeric near-infrared fluorescent proteins for multicolor imaging across scales. Nat Commun 11, 239 (2020..., V.V. Near-infrared fluorescent proteins for multicolor in vivo imaging. Nat Methods 10, 751-754 (2013...

... better in one configuration than the other (Sandomenico et al., 2020). Figure 1: Comparison between...that help scFvs fold properly in the cytoplasm (Sandomenico et al., 2020). conversion to a cysteine free ...253 . https://doi.org/10.1006/jmbi.1997.1457  Sandomenico A, Sivaccumar JP, Ruvo M (2020) Evolution of ...

...exchange vector. This vector contains sacB, an chloramphenicol resistance cassette, and the R6K origin of ...Valentovich 2014). We routinely clone the purified amplicons upstream and downstream of the desired deletion...products. To allow manipulation of naturally chloramphenicol-resistant strains, we have also included versions...

...NB, Brown M, Talbot D, Berg DE. pBR322-derived multicopy plasmids harboring large inserts are often dimers...self-control and a sense of direction are the secrets of multicopy plasmid stability. Mol Microbiol. 1998 Sep;29(...PMID: 9767582. Summers DK, Beton CW, Withers HL. Multicopy plasmid instability: the dimer catastrophe hypothesis...

...T-lymphocyte-associated protein 4 CD, CD152, CELIAC3, CTLA-4, GSE, ICOS, IDDM12 FOS v-fos FBJ murine osteosarcoma viral ...CTLO, H-RASIDX, HAMSV, HRAS1, K-RAS, N-RAS, RASH1 ICOS inducible T-cell co-stimulator AILIM, CD278, MGC39850...14, CST-17, CST-29 CRH corticotropin releasing hormone CRF CRHR1 corticotropin releasing hormone receptor...CRF-R, CRF1, CRFR1, CRH-R1h, CRHR, CRHR1f CRHR2 corticotropin releasing hormone receptor 2 CRFR2 CRIM1 cysteine...MSH-R, SHEP2 MC2R melanocortin 2 receptor (adrenocorticotropic hormone) ACTHR, MGC125798 MC3R melanocortin...MTNR1B melatonin receptor 1B MEL-1B-R, MT2 NAMPT nicotinamide phosphoribosyltransferase 1110035O14Rik, DKFZp666B131...nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor) GCCR, GCR, GR, GRL NR3C2 nuclear ...

... The system is compatible with both monocot and dicot plants. Liu Lab Golden Gate/Gibson Assembly Multiplexing...and plasmids are available for both monocot and dicot plants, with a choice of either hygromycin or Basta...high-efficiency multiplex genome editing in monocot and dicot plants.” Molecular Plant 8(8) (2015): 1274-1284....

... effective brightness and allow for single or multicolor imaging. Photo-activatable iRFPs can be ‘turned...empty backbone plasmids are ideal for single or multicolor in vivo imaging. Plasmids for In Vivo Imaging...

...Lentivectors Toolbox Optimized shRNA design in pSico with pSicoligomaker (Andrea Ventura lab) References Carthew...

...¡La primera edición de nuestro libro electrónico CRISPR 101 ya está disponible en español! Esta traducción...parte oficial de nuestra colección de libros electrónicos. Descargar CRISPR 101: Una herramienta de edición...