We narrowed to 206 results for: icos

...What’s a science writer?” Learning about SciComm by doing SciComm Writing had always been a strength of ...right place at the right time when she started her scicomm career: “ I think living in Washington D.C. helped... scicomm...

... Cuneate tract, dCST= dorsal cortico spinal tract, vCST= ventral cortico spinal tract, dc= decussation... first segments of the spinal cord showing the cortico spinal tract, CST, spinal cord and cranial nerve...device (EMCCD) or complementary metal-oxide semiconductor (CMOS) sensors, it takes only a few milliseconds...

...flanking the editing site on either side of the amplicon. Based on the frequency of out-of-frame edits...deleted accordingly. Pro tip: make sure your PCR amplicon isn’t too large so that you can easily visualize...expect off target editing to occur. Luckily, in silico prediction analyses like CRISPRitz or CRISPOR can...CRISPRitz: rapid, high-throughput and variant-aware in silico off-target site identification for CRISPR genome...

... you'll need Sanger sequencing reads from PCR amplicons that cover your locus of interest and correspond...intuitive interpretation of results produced by amplicon sequencing. The updated CRISPresso2 can analyze...

...little more this October. Science favorites Musicophilia: Tales of Music and the Brain by Oliver Sacks...properties it has. As with other works of Dr. Sacks, Musicophilia includes case studies of relevant neurological...book! Far from a work of glib pop psychology, Musicophilia is accessible and fascinating, all without compromising...

...Massachusetts Medical School developed a dCas9-based multicolor labeling system to visualize chromosomal loci... U2OS cells.   Figure 2: CRISPR-Sirius tricolor labeling. (A) Diagram of sgRNA-Sirius-8XMS2, sgRNA-Sirius...plasmids at Addgene. References Ma, Hanhui et al. “Multicolor CRISPR labeling of chromosomal loci in human ...

...Koen Venken 133315 pMKV060 Firefly AtUbi10 BeYDV replicon on T-DNA backbone expressing firefly luc for monitoring...Daniel Voytas 133312 pMKV057 Firefly AtUbi10 BeYDV replicon on T-DNA backbone expressing WUS2 and IPT, with...

...BioRender.com.   SLIC also opened up a new world of multicomponent assembly, as fragments with overlapping sequence...efficiency (~20%). Figure 2: Schematic of multicomponent assembly with SLIC. Multiple fragments are ...issue is sequence similarity. If fragments in a multicomponent assembly have 5′ or 3′ sequence homology to...

...Streptomyces , and others) Drosophila Plants (monocots and dicots) C. elegans Yeast ( S. cerevisiae and S. pombe ...Reyes-Gutierrez P, Wolfe SA, Zhang S, Pederson T. 2015. Multicolor CRISPR labeling of chromosomal loci in human ...

...codon-optimized SpCas9; sgRNA can be co-transfected as PCR amplicons (Ran et al., Nature Protocols 2013). Individual...SaCas9; SaCas9 sgRNA can be co-transfected as PCR amplicons using a protocol similar to SpCas9 sgRNA PCR (...

...Escherichia coli , Mycobacterium tuberculosis Vinay Nandicoori 78577 pL99 PnitA-NitR ε-caprolactam Escherichia...Escherichia coli , Mycobacterium tuberculosis Vinay Nandicoori 188974 pREDusk-AmpR-MCS FixK2 Red light (660 ...

...cell have the same replicon and/or partitioning system. Let’s start with the replicon- the part of the ...The replication problem Plasmids with the same replicon are incompatible because they compete for the ...

.... Use in silico cloning tools When available, always perform your MoClo assemblies in silico before you...Laboratory Golden Gate Cloning videos made by the Nicola Patron group, one of the developers of the MoClo...

...exchange vector. This vector contains sacB, an chloramphenicol resistance cassette, and the R6K origin of ...Valentovich 2014). We routinely clone the purified amplicons upstream and downstream of the desired deletion...products. To allow manipulation of naturally chloramphenicol-resistant strains, we have also included versions...

...These probes can be combined in two-color and multicolor NIR imaging.  Small miRFPnano A separate class...monomeric near-infrared fluorescent proteins for multicolor imaging across scales. Nat Commun 11, 239 (2020..., V.V. Near-infrared fluorescent proteins for multicolor in vivo imaging. Nat Methods 10, 751-754 (2013...

... better in one configuration than the other (Sandomenico et al., 2020). Figure 1: Comparison between...that help scFvs fold properly in the cytoplasm (Sandomenico et al., 2020). conversion to a cysteine free ...253 . https://doi.org/10.1006/jmbi.1997.1457  Sandomenico A, Sivaccumar JP, Ruvo M (2020) Evolution of ...

...NB, Brown M, Talbot D, Berg DE. pBR322-derived multicopy plasmids harboring large inserts are often dimers...self-control and a sense of direction are the secrets of multicopy plasmid stability. Mol Microbiol. 1998 Sep;29(...PMID: 9767582. Summers DK, Beton CW, Withers HL. Multicopy plasmid instability: the dimer catastrophe hypothesis...

... The system is compatible with both monocot and dicot plants. Liu Lab Golden Gate/Gibson Assembly Multiplexing...and plasmids are available for both monocot and dicot plants, with a choice of either hygromycin or Basta...high-efficiency multiplex genome editing in monocot and dicot plants.” Molecular Plant 8(8) (2015): 1274-1284....

...¡La primera edición de nuestro libro electrónico CRISPR 101 ya está disponible en español! Esta traducción...parte oficial de nuestra colección de libros electrónicos. Descargar CRISPR 101: Una herramienta de edición...

...teach students around the globe and is pushing SciComm further with his new company, Science with Tom.... scicomm...