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Showing: 41 - 60 of 980 results

  1. Trimmer Lab NeuroMab Collection

    Type
    Collection
    ...joining fragment comprising a κ light chain constant domain (C L ) and the κ light chain polyA tail sequences... for light chain expression, and an ER signal/leader sequence (L) for translocation of the light chain...in a new window) Durocher Y, et al. 2002. High-level and high-throughput recombinant protein production... R-mAbs are secreted into the culture media. For high level expression we recommend using the HEK-2936E...Link opens in a new window) . The schematic to the right shows the separate elements of the R-mAb expression...expression plasmid involved in coexpression of light (green) and heavy (blue) chains as driven by two CMV promoters...

  2. Antibody Production

    Type
    Collection
    ... for each specific antibody in order to generate high quality preparations. Following production, all ...transfected with the plasmid(s) encoding the heavy and light chains. Antibodies are expressed for 7 days and ...amplified with primers targeting the heavy chain and/or light chain variable regions. The PCR product is purified...

  3. Penn Vector Core Partnership with Addgene

    Type
    Collection
    ...Vector Core) and Addgene have partnered to provide high-quality AAV vectors to the academic research community...Deisseroth AV-1-36857M 100038-AAV1 pENN.AAV.hSynap.Flex.ArcLightDco.WPRE.SV40 Vincent Pieribone AV-1-36857P...36857P 100037-AAV1 pAAV.hSynap.ArcLightD.WPRE.SV40 Vincent Pieribone AV-1-51473 51473-AAV1 pAAV-hSyn1-mRuby2...James M. Wilson AV-1-PV2699 100030-AAV1 pAAV.CBA.loxP.ArcLightD.2A.nlsmCherry.loxP.WPRE.SV40 Lawrence Cohen...

  4. TALEN Guide

    Type
    Collection
    ... are able to recognize their targets with fairly high specificity. Fifteen years of research has shown...TALe research has been swift. The incentives are high. Since ZF targets are confined to sequences composed...on specificity is on par with ZF arrays, if not slightly lower. Figure 2: Simplified representation of ...

  5. Neurodegeneration Research Collection

    Type
    Collection
    ...and scFvs for neuroscience research created with high-volume hybridoma sequencing on the NeuroMabSeq platform...dissemination to the research community. This collection highlights plasmids created with support from the BRAIN ...

  6. Caltech Systemic Capsids

    Type
    Collection
    ...pAAV-EF1a-iCreV EF1a light-inducible recombinase iCreV Zeng 140136 pAAV-EF1a-iDreV EF1a light-inducible recombinase...recombinase iDreV Zeng 140137 pAAV-EF1a-iFlpV EF1a light-inducible recombinase iFlpV Zeng 164450 CN1851-rAAV-hI56i-minBglobin-iCre...

  7. Genomic Deletions in Mammalian Cell Lines

    Type
    Collection
    ... clones for loss of function. This is due to the high frequency of indels formed on non-deleted alleles...cells in order to enrich for cells that received high levels of the CRISPR/Cas9 constructs. Plate sorted...the CRISPR-Cas9 system. Ran FA, Hsu PD, Wright J, Agarwala V, Scott DA, Zhang F. Nature Protocols...

  9. TREAT-AD Plasmid Collection

    Type
    Collection
    ...resources for emerging targets in AD and creating high-quality, well-characterized lead molecules for prioritized...further translational investment. All resources and high-quality research tools are shared openly. Plasmids...

  10. CRISPR Plasmids - Empty gRNA Vectors

    Type
    Collection
    ... plasmid with or without expression of Cas9. Highlighted below are some multiplex gRNA vectors from our...Yang A Robust CRISPR/Cas9 System for Convenient, High-Efficiency Multiplex Genome Editing in Monocot and...

  11. DNA Service - Cloning Grade DNA

    Type
    Collection
    ...a variety of popular plasmids and backbones with high cloning potential. By making these plasmids available...spectrophotometer. Other methods of quantification may yield slightly different DNA concentrations. Can I directly transfect...

  12. Resolute Plasmid Collection

    Type
    Collection
    ... the results, techniques and reagents with the highest-possible quality of research output, RESOLUTE expects...production is used for assays and to generate antibody/ high-affinity ligands. Data is embedded in an integrated...

  13. Addgene Packaged on Request: Scope of Service

    Type
    Collection
    ...Addgene determines that our current queue is too high and we do not expect to meet the general turnaround...complete any additional customs documentation that might be required by your country for the import of biological...

  14. Synthetic Biology - Overview

    Type
    Collection
    ...pre-assembled genetic circuits such as logic gates and higher level gene networks. Sensing and Signaling Browse...Efflux Pumps Voigt Lab Repressors , Terminators , Light Signaling , Sigma Factors , Resource Allocator and...

  15. Adenovirus Plasmids

    Type
    Collection
    ...their large packaging capacity (>8 kb), high titers, and high levels of transgene expression. Additionally...

  16. Retrovirus Plasmids

    Type
    Collection
    ...γ-retroviruses. γ-Retroviruses can package relatively high amounts of DNA (up to ∼8 kb), and infection results...pRXTN CMV/MSV pRXTN is a modified version of pRX-tight Puro encoding NAT instead of PAC Iglehart 20672 ...

  17. Zinc Finger Consortium: Zinc Finger Arrays

    Type
    Collection
    ...Method Full Target Site (5' to 3') Left half-site Right half-site CACNA1F OZ507 and OZ508 OPEN OPEN gCGTGACCTCgctgatAAGAAGAGg...engineering of customized zinc-finger nucleases for highly efficient gene modification. Maeder ML et al. Mol...

  18. CRISPR Plasmids - Prime Edit

    Type
    Collection
    ... gRNA vectors suitable for prime editing are highlighted in the table below. Use the search bar to find...

  19. AAVED

    Type
    Collection
    ...consider when planning experiments). What resources might be useful to help improve this technology or its...are some current obstacles that researchers face right now (e.g., not enough protocols, cost, not enough...
Showing: 41 - 60 of 980 results